Research on the meaning of the enzymatic systems (GPI and PGM) as parameters for the definition of varieties (Vitis sp.): The Italian case of Cabernet franc

Several studies carried out at Davis and Conegliano showed that isozyme analysis of the GPI and PGM enzymatic systems agrees with the conventional definition of the variety in ampelography. Differences were reported among varieties but not among biotypes of the same varieties. The only exception recorded was in the population of Cabernet franc in which GPI and PGM reveal two different types (A - the traditional type encountered in France and B - type encountered in the Italian region of Veneto). Further ampelographic, ampelometric, phenological and chemical studies on the polyphenolic and aromatic substances in fruits have shown considerable differences between the two types. Such differences demonstrate that the type B is a different variety and not a clone of Cabernet franc. Preliminary ampelographic analysis and the equality in GPI and PGM patterns lead to the conclusion that the type B very probably is Carmenère.Therefore, the hypothesis of variety discrimination based on the analysis of GPI and PGM is valid and this method is useful to help to characterize the varieties

Dispersal of larval and juvenile seabream: Implications for Mediterranean marine protected areas

In the marine context, information about dispersal is essential for the design of networks of marine protected areas (MPAs). Generally, most of the dispersal of demersal fishes is thought to be driven by the transport of eggs and larvae in currents, with the potential contribution of dispersal in later life stages relatively minimal.Using otolith chemistry analyses, we estimate dispersal patterns across a spatial scale of approximately 180. km at both propagule (i.e. eggs and larvae) and juvenile (i.e. between settlement and recruitment) stages of a Mediterranean coastal fishery species, the two-banded seabream Diplodus vulgaris. We detected three major natal sources of propagules replenishing local populations in the entire study area, suggesting that propagule dispersal distance extends to at least 90. km. For the juvenile stage, we detected dispersal of up to 165. km. Our work highlights the surprising and significant role of dispersal during the juvenile life stages as an important mechanism connecting populations. Such new insights are crucial for creating effective management strategies (e.g. MPAs and MPA networks) and to gain support from policymakers and stakeholders, highlighting that MPA benefits can extend well beyond MPA borders, and not only via dispersal of eggs and larvae, but also through movement by juveniles

Identification alone versus intraoperative neuromonitoring of the recurrent laryngeal nerve during thyroid surgery: experience of 2034 consecutive patients

Background: The aim of this study was to evaluate the ability of intraoperative neuromonitoring in reducing the postoperative recurrent laryngeal nerve palsy rate by a comparison between patients submitted to thyroidectomy with intraoperative neuromonitoring and with routine identification alone. Methods: Between June 2007 and December 2012, 2034 consecutive patients underwent thyroidectomy by a single surgical team. We compared patients who have had neuromonitoring and patients who have undergone surgery with nerve visualization alone. Patients in which neuromonitoring was not utilized (Group A) were 993, patients in which was utilized (group B) were 1041. Results: In group A 28 recurrent laryngeal nerve injuries were observed (2.82%), 21 (2.11%) transient and 7 (0.7%) permanent. In group B 23 recurrent laryngeal nerve injuries were observed (2.21%), in 17 cases (1.63%) transient and in 6 (0.58%) permanent. Differences were not statistically significative. Conclusions: Visual nerve identification remains the gold standard of recurrent laryngeal nerve management in thyroid surgery. Neuromonitoring helps to identify the nerve, in particular in difficult cases, but it did not decrease nerve injuries compared with visualization alone. Future studies are warranted to evaluate the benefit of intraoperative neuromonitoring in thyroidectomy, especially in conditions in which the recurrent nerve is at high risk of injury. Keywords: Neuromonitoring, Recurrent laryngeal nerve, Thyroidectom

The uraemic hypertensive patient. a therapeutic challenge-right you are (if you think so)

High blood pressure (BP) is a leading cause of chronic kidney disease (CKD) and at the same time represents its most frequent complication. High BP is an independent risk factor for advanced CKD; on the other hand, at least 40% of patients with normal glomerular filtration rate (GFR) and virtually all patients with GFR <30 mL/min are hypertensive. CKD and microalbuminuria are powerful risk factors for cardiovascular morbidity and mortality. Consequently, in uraemic hypertension, it is of utmost importance to carefully manage both high BP and microalbuminuria, in order to slow down the progression of kidney damage and to reduce the incidence of cardiovascular events. The first purpose of the medical treatment in hypertensive patients is to normalize BP, regardless of the drug used. Nevertheless, some drugs have an 'additional' nephroprotective effect at the same BP target achieved. In this regard, first-line drugs are definitely renin-angiotensin-aldosterone inhibitors, mainly for their proved efficacy in reducing hypertension-related kidney damage and proteinuria. Anyway, a combined approach (two or more drugs) is usually needed to achieve the optimal BP target and reduce the worsening of CKD

Follicular nodules (Thy3) of the thyroid: is total thyroidectomy the best option?

BACKGROUND: Identification of the best management strategy for nodules with Thy3 cytology presents particular problems for clinicians. This study investigates the ability of clinical, cytological and sonographic data to predict malignancy in indeterminate nodules with the scope of determining the need for total thyroidectomy in these patients. METHODS: The study population consisted of 249 cases presenting indeterminate nodules (Thy3): 198 females (79.5%) and 51 males (20.5%) with a mean age of 52.43 ± 13.68 years. All patients underwent total thyroidectomy. RESULTS: Malignancy was diagnosed in 87/249 patients (34.9%); thyroiditis co-existed in 119/249 cases (47.79%) and was associated with cancer in 40 cases (40/87; 45.98%). Of the sonographic characteristics, only echogenicity and the presence of irregular margins were identified as being statistically significant predictors of malignancy. 52/162 benign lesions (32.1%) and 54/87 malignant were hypoechoic (62.07%); irregular margins were present in 13/162 benign lesions (8.02%), and in 60/87 malignant lesions (68.97%). None of the clinical or cytological features, on the other hand, including age, gender, nodule size, the presence of microcalcifications or type 3 vascularization, were significantly associated with malignancy. CONCLUSIONS: The rate of malignancy in cytologically indeterminate lesions was high in the present study sample compared to other reported rates, and in a significant number of cases Hashimoto’s thyroiditis was also detected. Thus, considering the fact that clinical and cytological features were found to be inaccurate predictors of malignancy, it is our opinion that surgery should always be recommended. Moreover, total thyroidectomy is advisable, being the most suitable procedure in cases of multiple lesions, hyperplastic nodular goiter, or thyroiditis; the high incidence of malignancy and the unreliability of intraoperative frozen section examination also support this preference for total over hemi-thyroidectomy

Correlation of vulnerability and damage between artistic assets and structural elements: The DataBAES archive for the conservation planning of CH masonry buildings in seismic areas

Historical buildings in seismic hazard-prone regions need specific measures in safety protection, largely due to the presence of artistic assets and/or decorations, both movable (e.g., statues, pinnacles, etc.) and unmovable (e.g., frescoes, valuable plasters or wall paintings, mosaics, and stuccoes). A correlation of damage between structural systems and artworks is fundamental for defining limit states, which can identify the proper conditions for interventions. Nevertheless, several vulnerability aspects can be identified before a seismic event occurs, the study of which can provide the basic dataset for setting up preventive measures in conservation programs. In this paper, the vulnerability and damage conditions related to structural elements (SE) and unmovable artistic assets (AA) belonging to historical masonry buildings are analysed. Optimized survey forms for the onsite detection of either intrinsic (e.g., compositional) defects or deterioration phenomena for both materials and structure are proposed, and results are provided in a web data system (called DataBAES). This enables us to compare the current levels of vulnerability and damage of AA and SE on a scale of five increasing grades. This procedure has been validated on a series of buildings struck by earthquakes in Italy and can be used for correlations of the seismic behaviour of SE and AA in predictive analyses

Cell-element simulations to optimize the performance of osmotic processes in porous membranes

We present a new module of the software tool PoreChem for 3D simulations of osmotic processes at the cell-element scale. We consider the most general fully coupled model (see e.g., Sagiv and Semiat (2011)) in 3D to evaluate the impact on the membrane performance of both internal and external concentration polarization, which occurs in a cell-element for different operational conditions. The model consists of the Navier–Stokes–Brinkman system to describe the free fluid flow and the flow within the membrane with selective and support layers, a convection–diffusion equation to describe the solute transport, and nonlinear interface conditions to fully couple these equations. First, we briefly describe the mathematical model and discuss the discretization of the continuous model, the iterative solution, and the software implementation. Then, we present the analytical and numerical validation of the simulation tool. Next, we perform and discuss numerical simulations for a case study. The case study concerns the design of a cell element for the forward osmosis experiments. Using the developed software tool we qualitatively and quantitatively investigate the performance of a cell element that we designed for laboratory experiments of forward osmosis, and discuss the differences between the numerical solutions obtained with the full 3D and reduced 2D models. Finally, we demonstrate how the software enables investigating membrane heterogeneities

Object detection in images using extended set of Haar-like features and histogram-based method

Tato práce je zaměřená na detekci objektů v obraze s využitím rozšířené sady hárových příznaků a histogramu orientovaných gradientů. Nejprve je uveden základní princip extrakce a klasifikace obrazových dat. V další části je představen vlastní koncept příznaku založený na metodě Diffusion distance. Výstupem této práce je implementace těchto metod jako operátory pro aplikaci Rapiminer.This diploma thesis is focused on detection in images using extended set of Haar-like features and histogram-based method. At first is introduced a basic concept of extraction and classification image features. The next part bring own concept of image features based on Diffusion distance. Result of this work is implementation this methods in Rapidminer.