Thermal duality and gravitational collapse

Thermal duality is a relationship between the behaviour of het-erotic string models of the E(8)xE(8) or SO(32) types at inversely related temperatures, a variant of T duality in the Euclidean regime. This duality would have consequences for the nature of the Hagedorn transition in these string models. We propose that the vacuum admits a family of deformations in situations where there are closed surfaces of constant area but high radial acceleration (a string regularized ver-sion of a Penrose trapped surface), such as would be formed in situ-ations of extreme gravitational collapse. This would allow a radical resolution of the firewall paradox by allowing quantum effects to sig-nificantly modify the spacetime geometry around a collapsed object. A string bremsstrahlung process would convert the kinetic energy of infalling matter in extreme gravitational collapse to form a region of the deformed vacuum, which would be equivalent to forming a high temperature string phase. A heuristic criterion for the conversion pro-cess is presented, relating Newtonian gravity to the string tension, suggesting an upper limit to the strength of the gravitational interac-tion. This conversion process might have observable consequences for charged particles falling into a rotating collapsed object by producing high energy particles via a variant of the Penrose process

Retinoic acid accelerates the specification of enteric neural progenitors from in-vitro-derived neural crest

The enteric nervous system (ENS) is derived primarily from the vagal neural crest, a migratory multipotent cell population emerging from the dorsal neural tube between somites 1 and 7. Defects in the development and function of the ENS cause a range of enteric neuropathies, including Hirschsprung disease. Little is known about the signals that specify early ENS progenitors, limiting progress in the generation of enteric neurons from human pluripotent stem cells (hPSCs) to provide tools for disease modeling and regenerative medicine for enteric neuropathies. We describe the efficient and accelerated generation of ENS progenitors from hPSCs, revealing that retinoic acid is critical for the acquisition of vagal axial identity and early ENS progenitor specification. These ENS progenitors generate enteric neurons in vitro and, following in vivo transplantation, achieved long-term colonization of the ENS in adult mice. Thus, hPSC-derived ENS progenitors may provide the basis for cell therapy for defects in the ENS

Mixing theory for culture and harvest in bioreactors of human mesenchymal stem cells on microcarriers

The use of human mesenchymal stem cells (hMSCs) in regenerative medicine is a potential major advance for the treatment of many medical conditions, especially with the use of allogeneic therapies where the cells from a single donor can be used to treat ailments in many patients. Such cells must be grown attached to surfaces and for large scale production, it is shown that stirred bioreactors containing ~200 μm particles (microcarriers) can provide such a surface. It is also shown that the just suspended condition, agitator speed NJS, provides a satisfactory condition for cell growth by minimizing the specific energy dissipation rate, εT, in the bioreactor whilst still meeting the oxygen demand of the cells. For the cells to be used for therapeutic purposes, they must be detached from the microcarriers before being cryopreserved. A strategy based on a short period (~7 min) of very high εT, based on theories of secondary nucleation, is effective at removing >99% cells. Once removed, the cells are smaller than the Kolmogorov scale of turbulence and hence not damaged. This approach is shown to be successful for culture and detachment in 4 types of stirred bioreactors from 15 mL to 5 L

Nucleosides rescue replication-mediated genome instability of human pluripotent stem cells

Human pluripotent stem cells (PSCs) are subject to the appearance of recurrent genetic variants on prolonged culture. We have now found that, compared with isogenic differentiated cells, PSCs exhibit evidence of considerably more DNA damage during the S phase of the cell cycle, apparently as a consequence of DNA replication stress marked by slower progression of DNA replication, activation of latent origins of replication, and collapse of replication forks. As in many cancers, which, like PSCs, exhibit a shortened G1 phase and DNA replication stress, the resulting DNA damage may underlie the higher incidence of abnormal and abortive mitoses in PSCs, resulting in chromosomal non-dysjunction or cell death. However, we have found that the extent of DNA replication stress, DNA damage, and consequent aberrant mitoses can be substantially reduced by culturing PSCs in the presence of exogenous nucleosides, resulting in improved survival, clonogenicity, and population growth

Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest

The enteric nervous system (ENS) is derived primarily from the vagal neural crest, a migratory multipotent cell population emerging from the dorsal neural tube between somites 1 and 7. Defects in the development and function of the ENS cause a range of enteric neuropathies, including Hirschsprung disease. Little is known about the signals that specify early ENS progenitors, limiting progress in the generation of enteric neurons from human pluripotent stem cells (hPSCs) to provide tools for disease modeling and regenerative medicine for enteric neuropathies. We describe the efficient and accelerated generation of ENS progenitors from hPSCs, revealing that retinoic acid is critical for the acquisition of vagal axial identity and early ENS progenitor specification. These ENS progenitors generate enteric neurons in vitro and, following in vivo transplantation, achieved long-term colonization of the ENS in adult mice. Thus, hPSC-derived ENS progenitors may provide the basis for cell therapy for defects in the ENS. In this article, Frith and colleagues show that retinoic acid (RA) signaling alters the axial identity of hPSC-derived neural crest cells in a time- and dose-dependent manner. They utilized this to derive enteric nervous system (ENS) proge

Optical symmetries and anisotropic transport in high-Tc superconductors

A simple symmetry analysis of in-plane and out-of-plane transport in a family of high temperature superconductors is presented. It is shown that generalized scaling relations exist between the low frequency electronic Raman response and the low frequency in-plane and out-of-plane conductivities in both the normal and superconducting states of the cuprates. Specifically, for both the normal and superconducting state, the temperature dependence of the low frequency $B_{1g}$ Raman slope scales with the $c-$axis conductivity, while the $B_{2g}$ Raman slope scales with the in-plane conductivity. Comparison with experiments in the normal state of Bi-2212 and Y-123 imply that the nodal transport is largely doping independent and metallic, while transport near the BZ axes is governed by a quantum critical point near doping $p\sim 0.22$ holes per CuO$_{2}$ plaquette. Important differences for La-214 are discussed. It is also shown that the $c-$ axis conductivity rise for $T\ll T_{c}$ is a consequence of partial conservation of in-plane momentum for out-of-plane transport.Comment: 16 pages, 8 Figures (3 pages added, new discussion on pseudogap and charge ordering in La214

Triangulation network of 1929-1944 of the first 1:500 urban map of València

[EN] Triangulation is a surveying method on which earlier maps made were based. Although the origins of the method can be traced back to the 16th century, it is still used today, with minor changes, to adjust networks observed with modern geodetic techniques. In this paper we present the geodetic survey work that was carried out for the primary triangulation network of the first 1:500 urban map of the city of València (Spain). It spanned from 1929 to 1944 and resulted in 421 maps covering about 174 square kilometres. We focus on four key elements to define the geometric framework of a map: (1) the geodetic network, (2) the cartographic projection, (3) the baseline measurements, and (4) the primary triangulation. The paper is based on the interpretation of original documents and field books recovered from the archives of the València City Council. In order to check the accuracy and consistency of the survey work, we recomputed all calculations directly from the field data, following the mathematical procedures of the time. We obtained a set of transformation parameters to convert the coordinates of 1929 to current coordinates based on the European Terrestrial Reference System of 1989 (ETRS89). Results showed that the 1929 primary triangulation angles and coordinates are accurate to 8 s of arc and 35 cm respectively, and that the coordinates transform well into the current reference system with average residuals of 26 cm across nine control points, demonstrating the high quality of the 1929 work.Villar-Cano, M.; Marqués-Mateu, Á.; Jiménez-Martínez, MJ. (2020). Triangulation network of 1929-1944 of the first 1:500 urban map of València. Survey Review (Online). 52(373):317-329. https://doi.org/10.1080/00396265.2018.1564599S31732952373Bitelli, G., Cremonini, S., & Gatta, G. (2014). Cartographic heritage: Toward unconventional methods for quantitative analysis of pre-geodetic maps. Journal of Cultural Heritage, 15(2), 183-195. doi:10.1016/j.culher.2013.04.003Blachut, T. J., Chrzanowski, A., & Saastamoinen, J. H. (1979). Urban Surveying and Mapping. doi:10.1007/978-1-4612-6145-2Brinker, R. C., & Minnick, R. (Eds.). (1987). The Surveying Handbook. doi:10.1007/978-1-4757-1188-2Gatta, G. 2010. Valorizzazione di cartografia storica attraverso moderne tecniche geomatiche: recupero metrico, elaborazione e consultazione in ambiente digitale [Valuation of historic cartography using modern geomatics techniques: metric recovering, making and use in digital environment]. Doctoral thesis. Bologna: Universitá di Bologna. 295 pages. (In Italian).Gorse, C., Johnston, D., & Pritchard, M. (2012). A Dictionary of Construction, Surveying and Civil Engineering. doi:10.1093/acref/9780199534463.001.0001Hotine, M. (1939). THE RE-TRIANGULATION OF GREAT BRITAIN IV—BASE MEASUREMENT. Empire Survey Review, 5(34), 211-225. doi:10.1179/sre.1939.5.34.211Kahmen, H., & Faig, W. (1988). Surveying. doi:10.1515/9783110845716Leick, A., Rapoport, L., & Tatarnikov, D. (2015). GPS Satellite Surveying. doi:10.1002/9781119018612Murdin, P. (2009). Full Meridian of Glory. doi:10.1007/978-0-387-75534-2Schofield, W., & Breach, M. (2007). Engineering Surveying. doi:10.1201/b12847Seeber, G. (2003). Satellite Geodesy. doi:10.1515/9783110200089Snyder, J. P. (1987). Map projections: A working manual. Professional Paper. doi:10.3133/pp139

The semiology of changing brand image

This article considers the attempted change to the image of an established brand by studying the semiotics within the brand’s historical advertising campaigns. The use of semiotics to study the interpretation of messages is discussed, and the link between interpretation of messages and advertising effectiveness in changing brand image is explored. The authors deconstruct advertisements of a brand to provide a model containing opposing dialectics that may aid managers by highlighting alternative symbolic messages contained in advertisements. Oncwe identified, these alternative symbolic messages may be used to help change brand image and influence advertising effectiveness. Although the study focuses upon a major brand of beer, this is an industry in which there are numerous small firms, and many of those have constrained marketing budgets, and thus need to make sure that their advertising is effective. Equally, entrepreneurial marketing is not to found only in the small firm, and the case study discusses a radical and imaginative brand repositioning of a well established product