A study of dead-end filtration enhanced by electric and acoustic fields

This thesis describes the experimental procedure and results of an investigation into the effect of electric and acoustic fields on dead end vacuum filtration. The test suspension used was low concentration titanium dioxide. Ultrasound energy was applied tangentially and electrical energy parallel to the filter medium. Varying electric field gradients were applied to the filter cell, either alone or together with the constant frequency acoustic field. The filter cell was based on a Nutsche filter, and allowed samples of cake to be taken at the end of the filtration experiment. Electric and acoustic field strengths, suspension characteristics and process parameters could all be varied independently. [Continues.

Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor mu 2 kinase

Endocytic cargo such as the transferrin receptor is incorporated into clathrin-coated pits by associating, via tyrosine-based motifs, with the AP2 complex. Cargo-AP2 interactions occur via the mu2 subunit of AP2, which needs to be phosphorylated for endocytosis to occur. The most likely role for mu2 phosphorylation is in cargo recruitment because mu2 phosphorylation enhances its binding to internalization motifs. Here, we investigate the control of mu2 phosphorylation. We identify clathrin as a specific activator of the mu2 kinase and, in permeabilized cells, we show that ligand sequestration, driven by exogenous clathrin, results in elevated levels of mu2 phosphorylation. Furthermore, we show that AP2 containing phospho-mu2 is mainly associated with assembled clathrin in vivo, and that the level of phospho-mu2 is strongly reduced in a chicken B cell line depleted of clathrin heavy chain. Our results imply a central role for clathrin in the regulation of cargo selection via the modulation of phospho-mu2 levels

Nanomechanical displacement detection using coherent transport in ordered and disordered graphene nanoribbon resonators

Graphene nanoribbons provide an opportunity to integrate phase-coherent transport phenomena with nanoelectromechanical systems (NEMS). Due to the strain induced by a deflection in a graphene nanoribbon resonator, coherent electron transport and mechanical deformations couple. As the electrons in graphene have a Fermi wavelength \lambda ~ a_0 = 1.4 {\AA}, this coupling can be used for sensitive displacement detection in both armchair and zigzag graphene nanoribbon NEMS. Here it is shown that for ordered as well as disordered ribbon systems of length L, a strain \epsilon ~ (w/L)^2 due to a deflection w leads to a relative change in conductance \delta G/G ~ (w^2/a_0L).Comment: 4 Pages, 4 figure

A phenomenological study of occupational engagement in recovery from mental illness

This study aimed to uncover the meaning of occupation for 13 people who self identified as being in recovery from mental illness. Recovery narratives were collected from the participants in a series of open ended conversational interviews that were recorded and transcribed. The interview transcripts were analysed using phenomenological and hermeneutic approaches, with a focus on participants’ descriptions of engagement in everyday occupations. A range of experiences were evident, from complete disengagement to complete absorption in occupations. Participants described significant shifts in their experience of time, space, body and other people while in different modes of occupation and these were captured under the themes of ‘non-doing’, ‘half-doing’, ‘engaged doing’ and ‘absorbed doing’. Each mode had the potential to support recovery by creating opportunities for participants to reconnect with aspects of their being-in-the-world. The findings highlight the dynamics at play in different modes of occupation and suggest that all forms of engagement, including disengagement, can be significant in the recovery process. A greater understanding of the dynamics of occupation for people experiencing mental illness will enable carers and mental health services to more effectively support people in their recovery

Casimir-Polder interaction between an atom and a dielectric slab

We present an explicit analytic calculation of the energy-level shift of an atom in front of a non-dispersive and non-dissipative dielectric slab. We work with the fully quantized electromagnetic field, taking retardation into account. We give the shift as a two-dimensional integral and use asymptotic analysis to find expressions for it in various retarded and non-retarded limiting cases. The results can be used to estimate the energy shift of an atom close to layered microstructures.Comment: 10 pages, incl 7 figure

Fully Analyzing an Algebraic Polya Urn Model

This paper introduces and analyzes a particular class of Polya urns: balls are of two colors, can only be added (the urns are said to be additive) and at every step the same constant number of balls is added, thus only the color compositions varies (the urns are said to be balanced). These properties make this class of urns ideally suited for analysis from an "analytic combinatorics" point-of-view, following in the footsteps of Flajolet-Dumas-Puyhaubert, 2006. Through an algebraic generating function to which we apply a multiple coalescing saddle-point method, we are able to give precise asymptotic results for the probability distribution of the composition of the urn, as well as local limit law and large deviation bounds.Comment: LATIN 2012, Arequipa : Peru (2012

The impact of the home musical environment on infants' language development

There is strong evidence that musical engagement influences children’s language development but little research has been carried out on the relationship between the home musical environment and language development in infancy. The current study assessed musical exposure at home (including parental singing) and language development in 64 infants (8.5 - 18 months). Results showed that the home musical environment significantly predicted gesture development. For a subgroup of infants’ below 12 months, both parental singing and overall home musical environment score significantly predicted word comprehension. These findings represent the first demonstration that an enriched musical environment in infancy can promote development of communication skill

Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells

After permeabilization with the pore-forming toxin streptolysin-O mast cells can be triggered to secrete by addition of both calcium and a GTP analogue. If stimulation is delayed after permeabilization, there is a progressive decrease in the extent of secretion upon stimulation, eventually leading to a complete loss of the secretory response. This loss of secretory response can be retarded by the addition of cytosol from other secretory tissues, demonstrating that the response is dependent on a number of cytosolic proteins. We have used this as the basis of a bioassay to purify Secernin 1, a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin 1 increases both the extent of secretion and increases the sensitivity of mast cells to stimulation with calcium