Juvenile tench ( Tinca tinca L.) response to practical diets with different replacement levels of fish meal by pea protein concentrate supplemented with methionine

.The effects of methionine supplementation in diets with different replacement levels of fish meal (FM) by pea protein concentrate (PPC) on survival, growth performance and body composition of juvenile tench (0.39 g of initial weight) were studied in a 90-day experiment. Six practical diets (50% crude protein) differing in replacement level of FM by PPC were tested: 0% (control diet), 35%,45%, 60%, 75% or 85%, corresponding to 0, 285, 366, 487, 608 and 685.4 g PPC kg−1 diet respectively. To provide the same amount as in control diet, methionine was included from the 45% substitution level. Survival rates were high, between 93.7% and 100%, without differences among treatments. Juveniles fed 75% and 85% of replacement diets showed lower (p < 0.05) weight and specific growth rate, which cannot be attributed to methionine deficiency. In all treatments, percentages of fish with externally visible deformities were low (under 0.1%). The increase in PPC diet content resulted in a significant reduction of lipid content in whole-body juvenile. Minimum methionine requirements for juvenile tench could be estimated in 10 g kg−1 diet and supplementation over this amount would be not necessary. Juvenile tench exhibited a high tolerance to PPC dietary content, up to 487 g kg−1, without affecting growth performance, which make possible to consider this vegetal source of protein as a suitable substitute to FM.S

Revista de Vertebrados de la Estación Biológica de Doñaña

Comportamiento reproductor del camaleón común (Chamaeleo chamaeleon L.) en el sur de EspañaDistribución de los reptiles en la provincia de Granada (SE. Península Ibérica)Datos sobre la reproducción y el crecimientode Psammodromus hispanicus Fitzinger, 1826 en un medio adehesado de la España CentralVariación en la colocación y orientación del nido del Alzacola (Cercotrichas galactotes) en dos especies de árbolesOrganización de la comunidad de aves reproductora en las landas montanas del País Vasco AtlánticoEcología de una población ibérica de lobos (Canis Lupus)Etude biométrique des Crosidures (Soricidae, Insectivora) de la región de Massa (Souss, Maroe).Variación geográfica del género Eliomys en la Península IbéricaTendencias gregarias del Ciervo (Cervus elaphus) en Doñana.Data on the autumn diet of the red deer (Cervus elaphus L. 1758) in the Montes de Toledo (Central Spain)Nota sobre la coexistencia de Hyla arborea (L. 1758E Hyla meridionalis (Boettger 1874) rn rl Valle del TiétarCalendario reproductivo y tamaño de las puesta en el galápago leproso, Mauremys leprosa (Shweigger, 1812), en Doñana, HuelvaPelícola (Felicola) inaqualis Piager, 1880 (MALLOPHAGA:TRICHODECTIDAE) parásito deE Herpestes ichneumon L (CARNIVORA: HERPESTIDAE)Abundancia y amplitud de los desplazamientos de Apodemus sylvaticus en cuatro biotopos de Doñana que difieren en cobertura vegetalPeer reviewe

High efficacy and tolerability of hepatitis C genotype 2 treatment with direct-acting antivirals in real world: analysis of the Hepa-C Registry

Poster presentatio

Efficacy and safety of HCV-treatment with direct-acting antiviral agents interferon-free, in patients with severe renal impairment in clinical practice

Abstrac

Doñana. Acta vertebrata. vol 17 (2)

Tres ejemplos de aplicación de métodos indirectos para la estimación de parametros poblacionales en cérvidoAlimentación de la garza real (Ardea cinerea) en la cuenca del Duero (España) durante el periodo reproductoDistribución y tamaño de colonias de buitre leonado (Gyps fulvus) en el alto Ebro y zona Cantábrica próximaDistribución y hábitat del visón americano (Mustela vison Schreber) en el sistema CentraRégimen alimentario del erizo (Erinaceus europaeus L.) durante verano y otoño en setos de la provincia de León (NO de España)Señales sociales de la espátula (Platalea leucorodia) durante el periodo reproductivoVariación del peso corporal en Arvicola terrestris (Rodentia, Arvicolidae) del nordeste ibéricoSobre el dimorfismo sexual en el cráneo del gato montés Felix silvestris Schreber, 1777 del sudoeste ibéricoUn nuevo núcleo poblacional de Apodemus flavicollis (Melchior, 1834) en la Península Ibérica.Selección de la lechuza común (Tyto alba) sobre el topillo común (Microtus duodecimcostatus)Importancia de la vegetación emergente en el comportamiento alimenticio de la fochaObservaciones sobre el papel del lagarto ocelado (Lacerta lepida Daudin), el erizo (Erinaceus europaeus L.) y el tejón (Meles meles L.) en la dispersión de semillasComentarios sobre los estimadores empleados en el método de la batida.El uso de la batida como método de censo: una replicaPeer reviewe

Deep-sequencing reveals broad subtype-specific HCV resistance mutations associated with treatment failure

A percentage of hepatitis C virus (HCV)-infected patients fail direct acting antiviral (DAA)-based treatment regimens, often because of drug resistance-associated substitutions (RAS). The aim of this study was to characterize the resistance profile of a large cohort of patients failing DAA-based treatments, and investigate the relationship between HCV subtype and failure, as an aid to optimizing management of these patients. A new, standardized HCV-RAS testing protocol based on deep sequencing was designed and applied to 220 previously subtyped samples from patients failing DAA treatment, collected in 39 Spanish hospitals. The majority had received DAA-based interferon (IFN) a-free regimens; 79% had failed sofosbuvir-containing therapy. Genomic regions encoding the nonstructural protein (NS) 3, NS5A, and NS5B (DAA target regions) were analyzed using subtype-specific primers. Viral subtype distribution was as follows: genotype (G) 1, 62.7%; G3a, 21.4%; G4d, 12.3%; G2, 1.8%; and mixed infections 1.8%. Overall, 88.6% of patients carried at least 1 RAS, and 19% carried RAS at frequencies below 20% in the mutant spectrum. There were no differences in RAS selection between treatments with and without ribavirin. Regardless of the treatment received, each HCV subtype showed specific types of RAS. Of note, no RAS were detected in the target proteins of 18.6% of patients failing treatment, and 30.4% of patients had RAS in proteins that were not targets of the inhibitors they received. HCV patients failing DAA therapy showed a high diversity of RAS. Ribavirin use did not influence the type or number of RAS at failure. The subtype-specific pattern of RAS emergence underscores the importance of accurate HCV subtyping. The frequency of “extra-target” RAS suggests the need for RAS screening in all three DAA target regions