Effects of Photoperiod, Low Temperature and N Nutrition on VSP Accumulation in Taproot of Alfalfa

In Medicago sativa L., vegetative storage protein (VSP), specifically accumulated in taproot, are strongly involved in nitrogen storage. How the accumulation of such VSPs is regulated remains largely unknown. Experiments were designed with non-nodulated alfalfa to determine if length of the photoperiod, a decrease of temperature, or high availability of mineral nitrogen may induce the accumulation of VSPs. 15N labelling was used to quantify nitrogen uptake and its further relative translocation within the plant while VSPs accumulation was analysed by ELISA quantification. Results showed that environmental factors such as shortening daylength or low temperature changed biomass allocation within the plant by reducing shoot growth. As a consequence, short days promoted the relative N allocation to taproot whereas VSP accumulation showed a higher trend. On the other hand, low temperature, changes in N source or availability in the nutrient solution, may lead to a higher influx of nitrogen and a higher soluble protein relative concentration in taproot while VSP abundance remained low

Is the remobilization of S and N reserves for seed filling of winter oilseed rape modulated by sulphate restrictions occurring at different growth stages?

How the remobilization of S and N reserves can meet the needs of seeds of oilseed rape subject to limitation of S fertilization remains largely unclear. Thus, this survey aims to determine the incidence of sulphate restriction [low S (LS)] applied at bolting [growth stage (GS) 32], visible bud (GS 53), and start of pod filling (GS 70) on source–sink relationships for S and N, and on the dynamics of endogenous/exogenous S and N contributing to seed yield and quality. Sulphate restrictions applied at GS 32, GS 53, and GS 70 were annotated LS32, LS53, and LS70. Long-term 34SO42− and 15NO3− labelling was used to explore S and N partitioning at the whole-plant level. In LS53, the sulphur remobilization efficiency (SRE) to seeds increased, but not enough to maintain seed quality. In LS32, an early S remobilization from leaves provided S for root, stem, and pod growth, but the subsequent demand for seed development was not met adequately and the N utilization efficiency (NUtE) was reduced when compared with high S (HS). The highest SRE (65±1.2% of the remobilized S) associated with an efficient foliar S mobilization (with minimal residual S concentrations of 0.1–0.2% dry matter) was observed under LS70 treatment, which did not affect yield components

Remobilization of leaf S compounds and senescence in response to restricted sulphate supply during the vegetative stage of oilseed rape are affected by mineral N availability

The impact of sulphur limitation on the remobilization of endogenous S compounds during the rosette stage of oilseed rape, and the interactions with N availability on these processes, were examined using a long-term 34SO42− labelling method combined with a study of leaf senescence progression (using SAG12/Cab as a molecular indicator) and gene expression of the transporters, BnSultr4;1 and BnSultr4;2, involved in vacuolar sulphate efflux. After 51 d on hydroponic culture at 0.3 mM 34SO42− (1 atom% excess), the labelling was stopped and plants were subject for 28 d to High S-High N (HS-HN, control), Low S-High N (LS-HN) or Low S-Low N (LS-LN) conditions. Compared with the control, LS-HN plants showed delayed leaf senescence and, whilst the shoot growth and the foliar soluble protein amounts were not affected, S, 34S, and SO42− amounts in the old leaves declined rapidly and were associated with the up-regulation of BnSultr4;1. In LS-LN plants, shoot growth was reduced, leaf senescence was accelerated, and the rapid S mobilization in old leaves was accompanied by decreased 34S and SO42−, higher protein mobilization, and up-regulation of BnSultr4;2, but without any change of expression of BnSultr4;1. The data suggest that to sustain the S demand for growth under S restriction (i) vacuolar SO42− is specifically remobilized in LS-HN conditions without any acceleration of leaf senescence, (ii) SO42− mobilization is related to an up-regulation of BnSultr4;1 and/or BnSultr4;2 expression, and (iii) the relationship between sulphate mobilization and up-regulation of expression of BnSultr4 genes is specifically dependent on the N availability

Effect of mineral sulphur availability on nitrogen and sulphur uptake and remobilization during the vegetative growth of Brassica napus L.

Because it has a high demand for sulphur (S), oilseed rape is particularly sensitive to S limitation. However, the physiological effects of S limitation remain unclear, especially during the rosette stage. For this reason a study was conducted to determine the effects of mineral S limitation on nitrogen (N) and S uptake and remobilization during vegetative growth of oilseed rape at both the whole-plant and leaf rank level for plants grown during 35 d with 300 μM 34SO42– (control plants; +S) or with 15 μM 34SO42– (S-limited plants; –S). The results highlight that S-limited plants showed no significant differences either in whole-plant and leaf biomas or in N uptake, when compared with control plants. However, total S and 34S (i.e. deriving from S uptake) contents were greatly reduced for the whole plant and leaf after 35 d, and a greater redistribution of endogenous S from leaves to the benefit of roots was observed. The relative expression of tonoplast and plasmalemma sulphate transporters was also strongly induced in the roots. In conclusion, although S-limited plants had 20 times less mineral S than control plants, their development remained surprisingly unchanged. During S limitation, oilseed rape is able to recycle endogenous S compounds (mostly sulphate) from leaves to roots. However, this physiological adaptation may be effective only over a short time scale (i.e. vegetative growth)

Mise en réserve d'azote sous forme de protéines de réserve des organes végétatifs (VSP)chez Medicago sativa L.et Brassica napus L. : principaux facteurs de régulation

*INRA UMR UCBN 950 EVA Ecophysiologie végétale, agronomie et nutritions NCS Caen (FRA) Diffusion du document : INRA UMR UCBN 950 EVA Ecophysiologie végétale, agronomie et nutritions NCS Caen (FRA) Diplôme : Dr. d'Universit

Nitrogen storage and remobilization in Brassica napus L. during the growth cycle

Oilseed rape (Brassica napus L.) is commonly grown for oil or bio-fuel production, while the seed residues can be used for animal feed. It can also be grown as a catch crop because of its efficiency in extracting mineral N from the soil profile. However, the N harvest index is usually low, due in part to a low ability to remobilize N from leaves and to the fall of N-rich leaves which allows a significant amount of N to return to the environment. In order to understand how N filling of pods occurs, experiments were undertaken to quantify N flows within the plant by 15N labelling. N uptake capacity decreased at flowering to a non significant level during pod filling. However, large amounts of endogenous N were transferred from the leaves to the stems and to taproots which acted as a buffering storage compartment later used to supply the reproductive tissues. About 15% of the total N cycling through the plant was lost through leaf fall and 48%, nearly all of which had been remobilized from vegetative tissues, was finally recovered in the mature pods. A 23 k Da polypeptide, accumulated in the taproots during flowering and later fully hydrolyzed to sustain grain filling, has been characterized as a vegetative storage protein (VSP). A mechanistic N uptake model based on the functioning of NO3- transport system has also been proposed. The overall results are discussed in relation to plant strategies which optimize N cycling to reproductive sinks by means of buffering vegetative tissues such as stems and taproots

Effects of environmental factors and endogenous signals on N uptake partitioning and taproot vegetative storage protein accumulation in Medicago sativa

International audienc

Short-day photoperiod induces changes in N uptake, N partitioning and accumulation of vegetative storage proteins in two Medicago sativa cultivars

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