134 research outputs found
Effect of ruminal ammonia supply on lysine utilization by growing steers
Citation: Hussein, A. H., Batista, E. D., Miesner, M. D., & Titgemeyer, E. C. (2016). Effect of ruminal ammonia supply on lysine utilization by growing steers. Journal of Animal Science, 94(2), 656-664. doi:10.2527/jas2015-9717Six ruminally cannulated Holstein steers (202 +/- 15 kg) were used to study the effects of ruminal ammonia loading on whole-body lysine (Lys) utilization. Steers were housed in metabolism crates and used in a 6 x 6 Latin square design. All steers received 2.52 kg DM/d of a diet (10.1% CP) containing 82% soybean hulls, 8% wheat straw, 5% cane molasses, and 5% vitamins and minerals, and 10 g/d of urea (considered to be part of the basal diet) was ruminally infused continuously to ensure adequate ruminal ammonia concentrations. All steers were ruminally infused continuously with 200 g/d of acetic acid, 200 g/d of propionic acid, and 50 g/d of butyric acid and abomasally infused with 300 g/d of glucose continuously to increase energy supply without increasing microbial protein supply. Steers were also abomasally infused continuously with an excess of all essential AA except Lys to ensure that Lys was the only limiting AA. Treatments were arranged as a 3 x 2 factorial with 3 levels of urea (0, 40, or 80 g/d) continuously infused ruminally to induce ammonia loading and 2 levels of Lys (0 or 6 g/d) continuously infused abomasally. Treatments did not affect fecal N output (P = 0.37). Lysine supplementation decreased (P < 0.01) urinary N excretion from 51.9 g/d to 44.3 g/d, increased (P < 0.01) retained N from 24.8 to 33.8 g/d, increased (P < 0.01) plasma Lys, and decreased (P <= 0.05) plasma serine, tyrosine, valine, leucine, and phenylalanine. Lysine supplementation also tended (P = 0.09) to reduce plasma urea-N. Urea infusions linearly increased (P = 0.05) retained N (27.1, 29.3, and 31.5 g/d) and also linearly increased (P < 0.01) urinary N excretion (31.8, 48.1, and 64.4 g/d), urinary urea (21.9, 37.7, and 54.3 g/d), urinary ammonia (1.1, 1.4, and 1.9 g/d), and plasma urea (2.7, 4.0, and 5.1 mM), and linearly decreased plasma alanine (P = 0.04) and plasma glycine (P < 0.01). Assuming that retained protein is 6.25 x retained N and contains 6.4% Lys, the incremental efficiencies of infused Lys utilization were 51%, 59%, and 69% for steers receiving 0, 40, and 80 g/d of urea, respectively, indicating that ruminal ammonia loads may improve the efficiency of Lys utilization. This is supported by observed increases in whole body-protein deposition in response to ammonia loading of our steers that were, by design, Lys deficient
Exact Eigenstates and Magnetic Response of Spin-1 and Spin-2 Vectorial Bose-Einstein Condensates
The exact eigenspectra and eigenstates of spin-1 and spin-2 vectorial
Bose-Einstein condensates (BECs) are found, and their response to a weak
magnetic field is studied and compared with their mean-field counterparts.
Whereas mean-field theory predicts the vanishing population of the zero
magnetic-quantum-number component of a spin-1 antiferromagnetic BEC, the
component is found to become populated as the magnetic field decreases. The
spin-2 BEC exhibits an even richer magnetic response due to quantum correlation
between 3 bosons.Comment: 5 pages, no figures. LaTeX20
Reconstructing bottom water temperatures from measurements of temperature and thermal diffusivity in marine sediments
Glacial isostatic adjustment reduces past and future Arctic subsea permafrost
Sea-level rise submerges terrestrial permafrost in the Arctic, turning it into subsea permafrost. Subsea permafrost underlies ~ 1.8 million km2 of Arctic continental shelf, with thicknesses in places exceeding 700 m. Sea-level variations over glacial-interglacial cycles control subsea permafrost distribution and thickness, yet no permafrost model has accounted for glacial isostatic adjustment (GIA), which deviates local sea level from the global mean due to changes in ice and ocean loading. Here we incorporate GIA into a pan-Arctic model of subsea permafrost over the last 400,000 years. Including GIA significantly reduces present-day subsea permafrost thickness, chiefly because of hydro-isostatic effects as well as deformation related to Northern Hemisphere ice sheets. Additionally, we extend the simulation 1000 years into the future for emissions scenarios outlined in the Intergovernmental Panel on Climate Change’s sixth assessment report. We find that subsea permafrost is preserved under a low emissions scenario but mostly disappears under a high emissions scenario
Effects of Guanidinoacetic Acid on Lean Growth and Methionine Flux in Cattle
Objective: To evaluate the effect of supplementing guanidinoacetic acid in the presence or absence of L-methionine on nitrogen retention (lean tissue growth) when cattle were purposefully maintained under conditions of a methionine deficiency.
Study Description: Seven ruminally-cannulated Holstein steers (355 lb) were used in an experiment where each steer received each of six treatments. Treatments were abomasal infusion of 0 or 6 g/day methionine, and 0, 7.5, or 15 g/day guanidinoacetic acid, with all combinations represented. Energy was supplied by ruminal infusion of volatile fatty acids and abomasal infusion of glucose. All essential amino acids except methionine were infused abomasally to make methionine the most limiting amino acid.
The Bottom Line: Supplementation with 15 g/day of guanidinoacetic acid tended to increase lean tissue growth when steers received supplemental methionine, probably by increasing creatine synthesis by the steers
Efficiency of lysine utilization by growing steers
Citation: Batista, E. D., Hussein, A. H., Detmann, E., Miesner, M. D., & Titgemeyer, E. C. (2016). Efficiency of lysine utilization by growing steers. Journal of Animal Science, 94(2), 648-655. doi:10.2527/jas2015-9716This study evaluated the efficiency of Lys utilization by growing steers. Five ruminally cannulated Holstein steers (165 +/- 8 kg) housed in metabolism crates were used in a 6 x 6 Latin square design; data from a sixth steer was excluded due to erratic feed intake. All steers were limit fed (2.46 kg DM/d), twice daily, diets low in RUP (81% soybean hulls, 8% wheat straw, 6% cane molasses, and 5% vitamins and minerals). Treatments were 0, 3, 6, 9, 12, and 15 g/d of L-Lys continuously abomasally infused. To prevent AA other than Lys from limiting performance, a mixture providing all essential AA to excess was continuously abomasally infused. Additional continuous infusions included 10 g urea/d, 200 g acetic acid/d, 200 g propionic acid/d, and 50 g butyric acid/d to the rumen and 300 g glucose/d to the abomasum. These infusions provided adequate ruminal ammonia and increased energy supply without increasing microbial protein supply. Each 6-d period included 2 d for adaptation and 4 d for total fecal and urinary collections for measuring N balance. Blood was collected on d 6 (10 h after feeding). Diet OM digestibility was not altered (P >= 0.66) by treatment and averaged 73.7%. Urinary N excretion was decreased from 32.3 to 24.3 g/d by increasing Lys supplementation to 9 g/d, with no further reduction when more than 9 g/d of Lys was supplied (linear and quadratic, P < 0.01). Changes in total urinary N excretion predominantly were due to changes in urinary urea N. Increasing Lys supply from 0 to 9 g/d increased N retention from 21.4 to 30.7 g/d, with no further increase beyond 9 g/d of Lys (linear and quadratic, P < 0.01). Break-point analysis estimated maximal N retention at 9 g/d supplemental Lys. Over the linear response surface of 0 to 9 g/d Lys, the efficiency of Lys utilization for protein deposition was 40%. Plasma urea N tended to be linearly decreased (P = 0.06) by Lys supplementation in agreement with the reduction in urinary urea N excretion. Plasma concentrations of Lys linearly increased (P < 0.001), but Leu, Ser, Val, and Tyr (P <= 0.02) were linearly reduced by Lys supplementation, likely reflecting increased uptake for protein deposition. In our model, Lys supplementation promoted significant increases in N retention and was maximized at 9 g/d supplemental Lys with an efficiency of utilization of 40%
Quantum Dynamics of Atomic Coherence in a Spin-1 Condensate: Mean-Field versus Many-Body Simulation
We analyse and numerically simulate the full many-body quantum dynamics of a
spin-1 condensate in the single spatial mode approximation. Initially, the
condensate is in a ``ferromagnetic'' state with all spins aligned along the
axis and the magnetic field pointing along the z axis. In the course of
evolution the spinor condensate undergoes a characteristic change of symmetry,
which in a real experiment could be a signature of spin-mixing many-body
interactions. The results of our simulations are conveniently visualised within
the picture of irreducible tensor operators.Comment: Accepted for publication for the special issue of "Optics
Communications" on Quantum Control of Light and Matte
Effects of limonene on ruminal fusobacterium necrophorum concentrations, fermentation, and lysine degradation in cattle
Citation: Samii, S. S., Wallace, N., Nagaraja, T. G., Engstrom, M. A., Miesner, M. D., Armendariz, C. K., & Titgemeyer, E. C. (2016). Effects of limonene on ruminal fusobacterium necrophorum concentrations, fermentation, and lysine degradation in cattle. Journal of Animal Science, 94(8), 3420-3430. doi:10.2527/jas2016-0455Previous in vitro data showed that Fusobacterium necrophorum was inhibited by limonene. We further evaluated effects of limonene on growth of F. necrophorum in vitro as well as on ruminal concentrations of F. necrophorum in vivo. With in vitro cultivation in anaerobic brain-heart infusion broth, limonene decreased growth of F. necrophorum. Thymol also reduced growth of F. necrophorum, but it was less effective than limonene. Tylosin effectively reduced growth of F. necrophorum in vitro. Although the response over fermentation times and concentrations of antimicrobials differed somewhat between tylosin and limonene, the 2 antimicrobial agents yielded similar inhibitory effects on growth of F. necrophorum at concentrations ranging from 6 to 24 mg/L. The effects of limonene on ruminal F. necrophorum concentration in vivo were tested in 7 ruminally cannulated heifers (225 kg initial BW) used in a 7 × 4 Youden square design. Treatments included: 1) control, 2) limonene at 10 mg/kg diet DM, 3) limonene at 20 mg/kg diet DM, 4) limonene at 40 mg/kg diet DM, 5) limonene at 80 mg/kg diet DM, 6) CRINA-L (a blend of essential oil components) at 180 mg/kg diet DM, and 7) tylosin at 12 mg/kg diet DM. Each period included 11 d with 10 d washouts between periods. Samples of ruminal contents were collected before treatment initiation and after 4, 7, and 10 d of treatment for measuring F. necrophorum by the most probable number method using selective culture medium. Limonene linearly decreased (P = 0.03) ruminal F. necrophorum concentration, with the lowest concentration achieved with 40 mg of limonene/kg dietary DM. Limonene tended (P ? 0.07) to linearly reduce ruminal molar proportions of propionate and valerate while tending to linearly increase (P ? 0.10) those of butyrate and 2-methyl butyrate. Limonene did not affect ruminal NH3 concentrations or degradation rates of lysine. Neither CRINA-L (P = 0.52) nor tylosin (P = 0.19) affected ruminal F. necrophorum concentrations. CRINA-L significantly decreased ruminal concentrations of NH3 and molar proportions of 3-methyl butyrate, whereas tylosin significantly decreased molar proportions of propionate while increasing those of butyrate and tending to increase those of acetate. Limonene supplementation reduced ruminal concentrations of F. necrophorum suggesting that it may have the potential to reduce the prevalence of liver abscesses, although further research is needed to assess the effect of limonene in feedlot cattle. © 2016 American Society of Animal Science. All rights reserved
Spin domain formation in spinor Bose-Einstein condensation
The spatial structure of the spinor Bose-Einstein condensates with the spin
degrees of freedom is analyzed based on the generalized Gross-Pitaevskii
equation (GP) in the light of the present spin domain experiment on m_F=\pm 1,
and 0 of the hyperfine state F=1 of ^{23}Na atom gases. The GP solutions in
three- and one-spatial dimensional cases reproduce the observed spin domain
structures, revealing the length scale associated with the existence of the
weak interaction of the spin-spin channel, other than the ordinary coherence
length related to the density-density channel. The obtained domain structure in
GP is compared with the result in Thomas-Fermi approximation. The former
solution is found to better describe the observed features than the latter.Comment: 9 pages, 14 figure
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