Immunoreactivity for a calmodulin-dependent protein kinase is selectively increased in macaque striate cortex after monocular deprivation

Immunocytochemical methods were used to localize type II Ca2+/calmodulin-dependent protein kinase in the macaque primary visual cortex. Neurons that stain for the kinase include both pyramidal and nonpyramidal cells and they appear to form a subset of cortical neurons. They are densely packed in layers II and IVB, somewhat more sparse in layers III, IVCß, and VI, and nearly absent in layer V. In normal animals the distribution of kinase-positive cells within each layer is relatively uniform. However, in animals in which one eye is removed 7-14 days before sacrifice or sutured shut for 9 or 11 weeks, the cells in layer IVCß are divided into alternating lightly and darkly stained bands. Comparison of immunocytochemically stained sections with adjacent sections stained for the mitochondrial enzyme, cytochrome oxidase, reveals that the kinase staining increases in ocular dominance columns originally driven by the removed or closed eye. These findings suggest that either the concentration of type II Ca2+/calmodulin-dependent protein kinase or its accessibility to the antibody probe increases dramatically and selectively in neurons of macaque primary visual cortex that have been deprived of their normal visual input. This may indicate that changing levels of activity in cortical neurons can alter their regulatory machinery

Gamma Ray Bursts: Cosmic Rulers for the High-Redshift Universe?

The desire to extend the Hubble Diagram to higher redshifts than the range of current Type Ia Supernovae observations has prompted investigation into spectral correlations in Gamma Ray Bursts, in the hope that standard candle-like properties can be identified. In this paper we discuss the potential of these new `cosmic rulers' and highlight their limitations by investigating the constraints that current data can place on an alternative Cosmological model in the form of Conformal Gravity. By fitting current Type 1a Supernovae and Gamma Ray Burst (GRB) data to the predicted luminosity distance redshift relation of both the standard Concordance Model and Conformal Gravity, we show that currently \emph{neither} model is strongly favoured at high redshift. The scatter in the current GRB data testifies to the further work required if GRBs are to cement their place as effective probes of the cosmological distance scale.Comment: 2 pages, 1 figure (black & white, colour available). To be published in "Phil. Trans. of the Royal Society" as proceedings from Discussion Meeting on Gamma Ray Burst

MObile Technology for Improved Family Planning: update to randomised controlled trial protocol.

BACKGROUND: This update outlines changes to the MObile Technology for Improved Family Planning study statistical analysis plan and plans for long-term follow-up. These changes result from obtaining additional funding and the decision to restrict the primary analysis to participants with available follow-up data. The changes were agreed prior to finalising the statistical analysis plan and sealing the dataset. METHODS/DESIGN: The primary analysis will now be restricted to subjects with data on the primary outcome at 4-month follow-up. The extreme-case scenario, where all those lost to follow-up are counted as non-adherent, will be used in a sensitivity analysis. In addition to the secondary outcomes outlined in the protocol, we will assess the effect of the intervention on long-acting contraception (implant, intra-uterine device and permanent methods).To assess the long-term effect of the intervention, we plan to conduct additional 12-month follow-up by telephone self-report for all the primary and secondary outcomes used at 4 months. All participants provided informed consent for this additional follow-up when recruited to the trial. Outcome measures and analysis at 12 months will be similar to those at the 4-month follow-up. The primary outcomes of the trial will be the use of an effective modern contraceptive method at 4 months and at 12 months post-abortion. Secondary outcomes will include long-acting contraception use, self-reported pregnancy, repeat abortion and contraception use over the 12-month post-abortion period. DISCUSSION: Restricting the primary analysis to those with follow-up data is the standard approach for trial analysis and will facilitate comparison with other trials of interventions designed to increase contraception uptake or use. Undertaking 12-month trial follow-up will allow us to evaluate the long-term effect of the intervention. TRIAL REGISTRATION: ClinicalTrials.gov NCT01823861

Evaluasi Penyimpangan Pondasi Tiang Dari Rencana Awal

Penelitian ini bertujuan untuk mencari ketepatan dalam pemancangan pondasi tiang dan membandingkan penyimpangan titik tiang pancang yang terjadi di lapangan dengan batasan standar-standar yang ada. Standar-standar yang digunakan untuk batasan toleransi penyimpangan pada tiang pancang adalah British Standard, Canadian Standard, dan Indian Standard. Ke-3 standar di atas menggunakan batas penyimpangan tiang pancang maksimum sebesar 75mm dan 150mm. Data yang menunjukan besarnya penyimpangan tiang pancang ditentukan dari hasil perpindahan tiang secara lateral dari posisi awal rencana tiang pancang tersebut. Hasil penelitian awal terhadap 19 proyek yang menggunakan pondasi tiang dengan dimensi dari 250mm sampai 600mm menunjukan bahwa sekitar 40% tiang dengan diameter 600mm berada dalam batas penyimpangan kurang dari 75mm, sedangkan sekitar 60% tiang dengan diameter 500mm, dan sekitar 65% tiang berdiameter 250mm sampai dengan 450mm. Dengan kata lain dapat disimpulkan bahwa semakin besar dimensi tiang semakin menurun tingkat ketepatan posisi tiang

Socio-Legal Studies in Germany and the UK: Theory and Methods

This Special Issue considers the situated and contextualized development of socio legal, or law and society, scholarship within two materially different legal and academic cultures, namely Germany and the United Kingdom, with a view to achieving a better understanding of why and how such differences in understanding and practice have arisen. The contributions are grouped into three themes. The first reflects upon the influence of institutional contexts and scholarly traditions in terms of the development of those approaches that come under the banner of socio legal studies. The second features contributions that adopt a comparative perspective in terms of selected areas of law, pointing to notably different approaches taken in Germany and the UK, and considering the development of these respective situations. The third looks at the key contemporary trends, theoretical applications, and methodological approaches taken within both countries’ socio legal academic contexts

Development of the Lymphoedema Genito-Urinary Cancer Questionnaire

The aim of this study was to develop a patient self-report tool to detect symptoms of genital and lower limb lymphoedema in male survivors of genitourinary cancer. The study incorporated the views of patients and subject specialists (lymphoedema and urology) in the design of a patient questionnaire based on the literature. Views on comprehensiveness, relevance of content, ease of understanding and perceived acceptability to patients were collated. The findings informed the development of the next iteration of the questionnaire. The overall view of participants was that the development and application of such a tool was of great clinical value and the Lymphoedema Genito- Urinary Cancer Questionnaire (LGUCQ) has significant potential for further development as a research tool to inform prevalence of this under-reported condition

Finite element analysis applied to redesign of submerged entry nozzles for steelmaking

The production of steel by continuous casting is facilitated by the use of refractory hollow-ware components. A critical component in this process is the submerged entry nozzle (SEN). The normal operating conditions of the SEN are arduous, involving large temperature gradients and exposure to mechanical forces arising from the flow of molten steel; experimental development of the components is challenging in so hazardous an environment. The effects of the thermal stress conditions in relation to a well-tried design were therefore simulated using a finite element analysis approach. It was concluded from analyses that failures of the type being experienced are caused by the large temperature gradient within the nozzle. The analyses pointed towards a supported shoulder area of the nozzle being most vulnerable to failure and practical in-service experience confirmed this. As a direct consequence of the investigation, design modifications, incorporating changes to both the internal geometry and to the nature of the intermediate support material, were implemented, thereby substantially reducing the stresses within the Al2O3/graphite ceramic liner. Industrial trials of this modified design established that the component reliability would be significantly improved and the design has now been implemented in series production