URBAN MICROCLIMATE IMPROVEMENT USING ENVI-MET CLIMATE MODEL

The aim of this paper is the modelling of urban microclimate, based on the limits imposed by the complexity of the three-dimensional space of cities. To this purpose, different Bioclimatic Scenarios were investigated through the microclimatic simulations using the micro-scale numerical model, ENVI-met 4v, applied in a case study of a Block in a highly residential neighbourhood of Athens. The study compares the bioclimatic scenarios of the roof top and road side vegetation plan in the current conditions, in order to evaluate how the existence of vegetation can affect the local air temperature and the thermal comfort condition of urban environment. This study also highlights the need to manage those microclimate data, through a geodatabase and provides a GIS approach of data organization and visualization. Creating building facades of the distributed temperature has showed that urban morphology parameters have an obvious impact on temperature distribution in the 3D space. On the other hand, the proposed roadside vegetation scenario has proved to be the most suitable way to improve the thermal comfort conditions of urban environment, as it can eliminate the Urban Heat Island (UHI) effects

A transcriptomic approach for evaluating the relative potency and mechanism of action of azoles in the rat Whole Embryo Culture.

We evaluated the effect of six azoles on embryonic development in the rat whole embryo culture (WEC). Using the total morphological scoring system (TMS), we calculated the ID10concentration (effective dose for 10% decrease in TMS). For evaluating gene specific responses, we combined previously and newly collected transcriptomics data of rat WEC exposed to a total of twelve azoles at their ID10for 4h. Results revealed shared expressions responses in genes involved in the retinoic acid (RA) and sterol biosynthesis pathways, which are respectively representatives of developmental toxicity and targeted fungicidal action of the azoles. Azoles with more pronounced effects on the regulation of RA-associated genes were generally characterized as more potent embryotoxicants. Overall, compounds with strong sterol biosynthesis related responses and low RA related responses were considered as more favourable candidates, as they specifically regulated genes related to a desired target response. Among the identified sterol associated genes, we detected that methylsterol monooxygenase 1 (Msmo1) was more sensitively induced compared to Cyp51, a classical biomarker of this pathway. Therefore, we suggest that Msmo1 could be a better biomarker for screening the fungicidal value of azoles. In summary, we conclude that the embryonic regulation of RA and sterol metabolic pathways could be indicators for ranking azoles as embryotoxicants and determining their drug efficacy