Black-pigmented anaerobic bacteria associated with ovine periodontitis

Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures

Chromosome evolution in Cophomantini (Amphibia, Anura, Hylinae)

The hylid tribe Cophomantini is a diverse clade of Neotropical treefrogs composed of the genera Aplastodiscus, Boana, Bokermannohyla, Hyloscirtus, and Myersiohyla. The phylogenetic relationships of Cophomantini have been comprehensively reviewed in the literature, providing a suitable framework for the study of chromosome evolution. Employing different banding techniques, we studied the chromosomes of 25 species of Boana and 3 of Hyloscirtus; thus providing, for the first time, data for Hyloscirtus and for 15 species of Boana. Most species showed karyotypes with 2n = 2x = 24 chromosomes; some species of the B. albopunctata group have 2n = 2x = 22, and H. alytolylax has 2n = 2x = 20. Karyotypes are all bi-armed in most species presented, with the exception of H. larinopygion (FN = 46) and H. alytolylax (FN = 38), with karyotypes that have a single pair of small telocentric chromosomes. In most species of Boana, NORs are observed in a single pair of chromosomes, mostly in the small chromosomes, although in some species of the B. albopunctata, B. pulchella, and B. semilineata groups, this marker occurs on the larger pairs 8, 1, and 7, respectively. In Hyloscirtus, NOR position differs in the three studied species: H. alytolylax (4p), H. palmeri (4q), and H. larinopygion (1p). Heterochromatin is a variable marker that could provide valuable evidence, but it would be necesserary to understand the molecular composition of the C-bands that are observed in different species in order to test its putative homology. In H. alytolylax, a centromeric DAPI+ band was observed on one homologue of chromosome pair 2. The band was present in males but absent in females, providing evidence for an XX/XY sex determining system in this species. We review and discuss the importance of the different chromosome markers (NOR position, C-bands, and DAPI/CMA3 patterns) for their impact on the taxonomy and karyotype evolution in Cophomantini

Effect of Fibrin Glue on the Biomechanical Properties of Human Descemet's Membrane

10.1371/journal.pone.0037456PLoS ONE75

Management of Type 2 Bubble Formed During Big-Bubble Deep Anterior Lamellar Keratoplasty

Corrispondig letter related to Management of Type 2 Bubble Formed During Big-Bubble Deep Anterior Lamellar Keratoplast

Deep suturing technique for penetrating keratoplasty

Purpose. To evaluate the effect of a new suturing technique on postkeratoplasty visual rehabilitation time and refractive error. Methods. Penetrating keratoplasty was performed on 17 eyes with keratoconus using a modified suturing technique. A donor button 8.0 mm in diameter was sutured into a 7.75-mm recipient bed with both deep and superficial sutures. The deep sutures consisted of either a single 16-bite 10-0 nylon running suture (n = 7) or eight interrupted 10-0 nylon cross-stitches (n = 10). These sutures were passed into the mid-stroma of the donor cornea and exited through the donor endothelium, then passed through the endothelium of the recipient cornea and exited from its mid-stroma. Thereby all parts of the deep sutures remained below the corneal surface. To further secure the surgical wound, in each case a running 16-bite 10-0 nylon superficial suture was also placed. Care was taken to maintain the bites of the superficial suture above the level of the deep sutures. The superficial suture was removed 3 months after surgery. Vision and refraction were recorded 1 day and 1 month postoperatively and 1 and 3 months after suture removal. A paired Student t test was used to verify the significance of changes in visual acuity and refraction recorded at different examination times. Results. As early as 1 month after surgery, spectacle best-corrected visual acuity 20/40 or more and refractive astigmatism less than 4 diopters (D) were recorded in each eye and maintained with two exceptions at the later examination times. In two patients, postoperative astigmatism increased from 4.5 to 5 D after suture removal. Conclusions. Deep suturing allowed quick visual rehabilitation while minimizing postkeratoplasty astigmatism in the patients with keratoconus operated on in this series

Amphibia, Anura, Cycloramphidae, Proceratophrys bigibbosa: distribution extension for Rio Grande do Sul, Brazil

Non

Microkeratome-assisted mushroom keratoplasty with minimal endothelial replacement

PURPOSE: To report the outcome of a new keratoplasty technique aimed at treating full-thickness opacities with minimal removal of recipient endothelium. DESIGN: Interventional case report. METHODS: A deep central scar was removed using microkeratome-assisted mushroom-shaped keratoplasty, consisting of a large anterior stromal lamella (9.0-mm in diameter) and a small posterior button (5.0-mm in diameter) including deep stroma and endothelium. Complete suture removal was performed 3 months after surgery. Visual acuity, refraction, and computerized corneal topography were evaluated pre- and postoperatively. RESULTS: Best spectacle-corrected visual acuity improved from 20/60 to 20/20 at 6 months postoperatively. Postoperative refraction was -2.50-1.00 à 20 degrees. CONCLUSIONS: Microkeratome-assisted mushroom keratoplasty may offer visual and refractive advantages over conventional keratoplasty surgery. Because most of the recipient endothelium is preserved and may spread onto the posterior surface of the small donor button, graft decompensation secondary to immunologic rejection may be avoided with this technique. © 2005 by Elsevier Inc. All rights reserved

ORBITAIMPLANTATE AUS KORALLINEM HYDROXYLAPATIT ALS BULBUSERSATZ NACH ENUKLEATION

Background: During the last two decades, hydroxyapatite has become popular as alloplastic material for bone replacement in orthopedic and dental surgery. We investigated prospectively the feasibility of hydroxyapatite spheres as replacement of enucleated eyes. Patients and Methods: Thirty-three consecutive patients entered a prospective study designed to evaluate the results obtained after either primary (n = 26) or secondary (n = 7) implantation of a hydroxyapatite sphere as replacement of the enucleated eyeball. Each patient was examined preoperatively, 1, 2, and 4 months after surgery, as well as every 6 months thereafter. At each examination time the motility of both coralline sphere and overlying cosmetic prothesis was evaluated and the conjunctival sac was carefully examined at the slit-lamp. Results: The postoperative follow-up averaged 10.5 months for the primary implantations and 7.2 months for the secondary implantations. Two implants had to be removed because of infection unresponsive to antibiotic treatment. Defects in the conjunctiva overlying the implant necessitated wound revision in two cases. A buccal mucosal graft was performed in two additional ones. A temporary cosmetic prothesis could be fitted in all but two of the remaining patients as early as 3 weeks after surgery. Motility of at least 3 mm in all directions could be obtained in 31 cases. Conclusion: Our study demonstrated that satisfactory motility and good biocompatibility can be achieved, at least in the medium term, with implantation of coralline hydroxyapatite spheres