Novel, Real-Time Cell Analysis for Measuring Viral Cytopathogenesis and the Efficacy of Neutralizing Antibodies to the 2009 Influenza A (H1N1) Virus

A novel electronic cell sensor array technology, the real-time cell analysis (RTCA) system, was developed to monitor cell events. Unlike the conventional methods labeling the target cells with fluorescence, luminescence, or light absorption, the RTCA system allows for label-free detection of cell processes directly without the incorporation of labels. Here, we used this new format to measure the cytopathic effect (CPE) of the 2009 influenza A (H1N1) virus and the efficacy of neutralizing antibodies in human sera to this virus. The real-time dynamic monitoring of CPE was performed on MDCK cell cultures infected with the H1N1 virus, ranging from 5.50×102 to 5.50×107 copies/mL. The resulting CPE kinetic curves were automatically recorded and were both time and viral load dependent. The CPE kinetics were also distinguishable between different H1N1 stains, as the onset of CPE induced by the A/Shanghai/37T/2009 H1N1 virus was earlier than that of the A/Shanghai/143T/2009 H1N1 virus. Furthermore, inhibition of H1N1 virus-induced CPE in the presence of human specific anti-sera was detected and quantified using the RTCA system. Antibody titers determined using this new neutralization test correlated well with those obtained independently via the standard hemagglutination inhibition test. Taken together, this new CPE assay format provided label-free and high-throughput measurement of viral growth and the effect of neutralizing antibodies, illustrating its potential in influenza vaccine studies

SN 2020udy: A new piece of the homogeneous bright group in the diverse Iax subclass

We present optical observations and analysis of a bright type Iax SN~2020udy hosted by NGC 0812. The light curve evolution of SN~2020udy is similar to other bright Iax SNe. Analytical modeling of the quasi bolometric light curves of SN 2020udy suggests that 0.08$\pm$0.01 M$_{\odot}$ of $^{56}$Ni would have been synthesized during the explosion. Spectral features of SN 2020udy are similar to the bright members of type Iax class showing weak Si {\sc II} line. The late-time spectral sequence is mostly dominated by Iron Group Elements (IGEs) with broad emission lines. Abundance tomography modeling of the spectral time series of SN~2020udy using TARDIS indicates stratification in the outer ejecta, however, to confirm this, spectral modeling at a very early phase is required. After maximum light, uniform mixing of chemical elements is sufficient to explain the spectral evolution. Unlike the case of normal type Ia SNe, the photospheric approximation remains robust until +100 days, requiring an additional continuum source. Overall, the observational features of SN 2020udy are consistent with the deflagration of a Carbon-Oxygen white dwarf.Comment: 18 pages, 17 figures, 3 tables, Accepted for publication in Ap

Antibiotic prescribing for upper respiratory infections among children in rural China: a cross-sectional study of outpatient prescriptions

Background: Overuse of antibiotics contributes to the development of antimicrobial resistance. Objective: This study aims to assess the condition of antibiotic use at health facilities at county, township and village levels in rural Guangxi, China. Methods: We conducted a cross-sectional study of outpatient antibiotic prescriptions in 2014 for children aged 2–14 years with upper respiratory infections (URI). Twenty health facilities were randomly selected, including four county hospitals, eight township hospitals and eight village clinics. Prescriptions were extracted from the electronic records in the county hospitals and paper copies in the township hospitals and village clinics. Results: The antibiotic prescription rate was higher in township hospitals (593/877, 68%) compared to county hospitals (2736/8166, 34%) and village clinics (96/297, 32%) (p < 0.001). Among prescriptions containing antibiotics, county hospitals were found to have the highest use rate of broad-spectrum antibiotics (82 vs 57% [township], vs 54% [village], p < 0.001), injectable antibiotics (65 vs 43% [township], vs 33% [village], p < 0.001) and multiple antibiotics (47 vs 15% [township], vs 0% [village], p < 0.001). Logistic regression showed that the likelihood of prescribing an antibiotic was significantly associated with patients being 6–14 years old compared with being 2–5 years old (adjusted odds ratio [aOR] = 1.3, 95% CI 1.2–1.5), and receiving care at township hospitals compared with county hospitals (aOR = 5.0, 95% CI 4.1–6.0). Prescriptions with insurance copayment appeared to lower the risk of prescribing antibiotics compared with those without (aOR = 0.8, 95% CI 0.7–0.9). Conclusions: Inappropriate use of antibiotics was high for outpatient childhood URI in the four counties of Guangxi, China, with the highest rate found in township hospitals. A significant high proportion of prescriptions containing antibiotics were broad-spectrum, by intravenous infusion or with multiple antibiotics, especially at county hospitals. Urgent attention is needed to address this challenge

Energetic eruptions leading to a peculiar hydrogen-rich explosion of a massive star

Every supernova so far observed has been considered to be the terminal explosion of a star. Moreover, all supernovae with absorption lines in their spectra show those lines decreasing in velocity over time, as the ejecta expand and thin, revealing slower-moving material that was previously hidden. In addition, every supernova that exhibits the absorption lines of hydrogen has one main light-curve peak, or a plateau in luminosity, lasting approximately 100 days before declining1. Here we report observations of iPTF14hls, an event that has spectra identical to a hydrogen-rich core-collapse supernova, but characteristics that differ extensively from those of known supernovae. The light curve has at least five peaks and remains bright for more than 600 days; the absorption lines show little to no decrease in velocity; and the radius of the line-forming region is more than an order of magnitude bigger than the radius of the photosphere derived from the continuum emission. These characteristics are consistent with a shell of several tens of solar masses ejected by the progenitor star at supernova-level energies a few hundred days before a terminal explosion. Another possible eruption was recorded at the same position in 1954. Multiple energetic pre-supernova eruptions are expected to occur in stars of 95 to 130 solar masses, which experience the pulsational pair instability2,3,4,5. That model, however, does not account for the continued presence of hydrogen, or the energetics observed here. Another mechanism for the violent ejection of mass in massive stars may be required

Sustainable nutrient substrates for enhanced seedling development in hydroponics

Sustainable agriculture can be achieved by upcycling and repurposing organic wastes for high-value applications. Keratin and cellulose are two natural biopolymers which are plentiful in biowastes such as hair, poultry feathers, wood shavings, and vegetable trimmings. In this study, these waste-derived biopolymers are converted into bioactive nutrient substrates that can support crop development in hydroponic culture systems. Keratin extracted from human hair (HHK) and cellulose nanofibers (CNFs) obtained from wood pulp were fabricated into composite substrates by freeze-drying. The substrates exhibited highly microporous structures, superior hydrophilicity, and excellent mechanical resilience. The obtained substrates not only serve as a physical carrier to support seed germination and seedling development but also function as advanced nutrient delivery platforms by the incorporation and controlled release of micronutrient-doped carbon dots, in addition to keratin degradation. Functional experiments using the model plant Arabidopsis and crops including Bok Choy (Brassica rapa) and Arugula (Eruca vesicaria) indicated that these substrates have the potential to be customized for enhanced seedling development in comparison to conventional substrates. This study demonstrates the feasibility and potential of upcycling and repurposing keratinous and cellulosic wastes to provide a sustainable solution for targeted nutrient delivery to crops.Ministry of Education (MOE)Nanyang Technological UniversityNational Research Foundation (NRF)Published versionThis research is supported by Nanyang Technological University-Harvard T. H. Chan School of Public Health Initiative for Sustainable Nanotechnology (NTU-HSPH18004) the Ministry of Education, Singapore (AcRF Tier 1 RT10/20), and the National Research Foundation, Prime Minister’s Office, Singapore, under its Campus for Research Excellence And Technological Enterprise (CREATE) and SHARE (Singapore HUJ Alliance for Research and Enterprise) program [REQ0246254 (NRF2020-THE003-0008)]

Urban and Rural Differences of Acute Cardiovascular Disease Events: A Study from the Population-Based Real-Time Surveillance System in Zhejiang, China in 2012.

Zhejiang province, China, has implemented a population based, real-time surveillance system that tracks acute cardiovascular diseases (CVDs) events since 2001. This study aimed to describe the system and report CVD incidence, mortality and case-fatality between urban and rural areas in Zhejiang in 2012. The surveillance system employs a stratified random sampling method covering all permanent residents of 30 counties/districts in Zhejiang. Acute CVD events such as coronary heart disease (CHD) and stroke were defined, registered and reviewed based on the adapted MONICA (Monitoring Trends and Determinants in Cardiovascular Disease) definitions. Data were collected from health facilities, vital registries, supplementary surveys, and additional investigations, and were checked for data quality before input in the system. We calculated the rates and compared them by gender, age and region. In 2012, the incidence, mortality and case-fatality of total acute CVD events were 367.0 (CHD 59.1, stroke 307.9), 127.1 (CHD 43.3, stroke 83.8) per 100,000 and 34.6% (CHD 73.2%, stroke 27.2%), respectively. Compared with rural areas, urban areas reported higher incidence and mortality but lower case-fatality rates for CHD (P<0.001), while lower incidence but higher mortality and case-fatality rates for stroke (P<0.001). We found significant differences on CHD and stroke epidemics between urban and rural areas in Zhejiang. Special attentions need to be given to stroke control, especially in rural areas

Populations (in millions) and percentage distribution by demographic characteristics in the surveillance sites, compared with two reference populations Zhejiang province and China, 2012.

<p>Populations (in millions) and percentage distribution by demographic characteristics in the surveillance sites, compared with two reference populations Zhejiang province and China, 2012.</p

Geographical distribution of the 30 surveillance sites in Zhejiang province, China.

<p>Geographical distribution of the 30 surveillance sites in Zhejiang province, China.</p