The new ALEPH Silicon Vertex Detector

The ALEPH collaboration, in view of the importance of effective vertex detection for the Higgs boson search at LEP 2, decided to upgrade the previous vertex detector. Main changes were an increased length (±20 cm), a higher granularity for rφ view (50 µm), a new preamplifier (MX7 rad hard chip), a polymide (upilex) fan-out on z side to carry the signals from the strips to the front-end electronics outside the fiducial region reducing consequently the passive material in the central region by a factor of two. The detector, the running experience and its performance will be described

The new ALEPH Silicon Vertex Detector

The ALEPH collaboration, in view of the importance of effective vertex detection for the Higgs boson search at LEP 2, decided to upgrade the previous vertex detector. Main changes were an increased length (±20 cm), a higher granularity for rφ view (50 µm), a new preamplifier (MX7 rad hard chip), a polymide (upilex) fan-out on z side to carry the signals from the strips to the front-end electronics outside the fiducial region reducing consequently the passive material in the central region by a factor of two. The detector, the running experience and its performance will be described

Heterologous expression of a thermophilic diacylglycerol acyltransferase triggers triglyceride accumulation in Escherichia coli

Triglycerides (TAGs), the major storage molecules of metabolic energy and source of fatty acids, are produced as single cell oil by some oleogenic microorganisms. However, these microorganisms require strict culture conditions, show low carbon source flexibilities, lack efficient genetic modification tools and in some cases pose safety concerns. TAGs have essential applications such as behaving as a source for added-value fatty acids or giving rise to the production of biodiesel. Hence, new alternative methods are urgently required for obtaining these oils. In this work we describe TAG accumulation in the industrially appropriate microorganism Escherichia coli expressing the heterologous enzyme tDGAT, a wax ester synthase/triacylglycerol:acylCoA acyltranferase (WS/DGAT). With this purpose, we introduce a codon-optimized gene from the thermophilic actinomycete Thermomonospora curvata coding for a WS/DGAT into different E. coli strains, describe the metabolic effects associated to the expression of this protein and evaluate neutral lipid accumulation. We observe a direct relation between the expression of this WS/DGAT and TAG production within a wide range of culture conditions. More than 30% TAGs were detected within the bacterial neutral lipids in 90 minutes after induction. TAGs were observed to be associated with the hydrophobic enzyme while forming round intracytoplasmic bodies, which could represent a bottleneck for lipid accumulation in E. coli. We detected an increase of almost 3- fold in the monounsaturated fatty acids (MUFA) occurring in the recombinant strains. These MUFA were predominant in the accumulated TAGs achieving 46% of the TAG fatty acids. These results set the basis for further research on the achievement of a suitable method towards the sustainable production of these neutral lipids

Lipid droplets: a classic organelle with new outfits

Lipid droplets are depots of neutral lipids that exist virtually in any kind of cell. Recent studies have revealed that the lipid droplet is not a mere lipid blob, but a major contributor not only to lipid homeostasis but also to diverse cellular functions. Because of the unique structure as well as the functional importance in relation to obesity, steatosis, and other prevailing diseases, the lipid droplet is now reborn as a brand new organelle, attracting interests from researchers of many disciplines

Femtosecond laser written superimposed fiber Bragg gratings for strain independent 3D shape sensing

Fiber Bragg gratings are inscribed around the edge of a standard single mode fiber core by femtosecond laser technology. By analyzing the back reflected light intensities, a novel and precise 3D shape sensor is realized

Direct inscription and evaluation of fiber Bragg gratings in carbon-coated optical sensor glass fibers for harsh environment oil and gas applications

In this research work, we show the successful inscription of fiber Bragg gratings into carbon-coated pure silica as well as germanium-doped glass fibers by applying the pulsed laser point-by-point manufacturing technique. First, the parameters used for the Ti:sapphire femtosecond laser process are demonstrated. Without removing the polymeric carbon coating, destruction-free formation of highly reflective Bragg gratings is performed with selected types of hermetically enclosed fibers. We demonstrate the advantage of the carbon coating by long-term exposure to a pure hydrogen atmosphere at an elevated temperature. Such harsh conditions exist in the oil and gas industry, which means there is high application potential for technologically advanced optical sensors. Compared to the also examined standard glass fibers with a distinct signal attenuation, carbon-coated fibers show no significant degradation. Finally, we analyze the mechanical stability of the processed fibers via standardized tensile tests. No substantial decrease in strength occurs among the sensor-integrated samples

Fiber-optical 3D shape sensing

Fiber Bragg grating (FBG) technology is well known since more than three decades. It started in 1978 with the discovery of photosensitivity in optical fibers by Ken Hill et al. [1] when illuminating germanium-doped silica fibers with visible argon ion laser radiation. In this context, first periodic refractive index variation was introduced into the core of such special optical fibers. However, for nearly one decade, there was found no real application of these fundamental observations. The major breakthrough for Bragg gratings came in 1988 with the report on holographic writing applying single-photon absorption in the ultraviolet by Metz et al. [2]. They demonstrated reflection gratings using two interfering laser beams imaged into the fiber core. This was the starting point for several applications of FBGs ranging from reflection gratings used in telecommunication, high reflectivity end reflectors in fiber lasers, or sensor applications for monitoring mechanical strain and temperature

Femtosecond laser written cladding waveguides and gratings for 3D shape sensing applications

Femtosecond laser pulses are used to inscribe multiple evanescent coupling waveguides with Fiber Bragg gratings in the cladding of single mode fibers to create a fiberoptical 3D shape sensor in one production step. An evaluation and a sample application will be presented

Integration of fiber optical shape sensing with medical visualization for minimal-invasive interventions

We present a fiber optical shape sensing system that allows to track the shape of a standard telecom fiber with fiber Bragg grating. The shape sensing information is combined with a medical visualization platform to visualize the shape sensing information together with medical images and post-processing results like 3D models, vessel graphs, or segmentation results. The framework has a modular nature to use it for various medical applications like catheter or needle based interventions. The technology has potential in the medical area as it is MR-compatible and can easily be integrated in catheters and needles due to its small size