16 research outputs found
Non-perturbative electron dynamics in crossed fields
Intense AC electric fields on semiconductor structures have been studied in
photon-assisted tunneling experiments with magnetic field applied either
parallel (B_par) or perpendicular (B_per) to the interfaces. We examine here
the electron dynamics in a double quantum well when intense AC electric fields
F, and tilted magnetic fields are applied simultaneously. The problem is
treated non-perturbatively by a time-dependent Hamiltonian in the effective
mass approximation, and using a Floquet-Fourier formalism. For B_par=0, the
quasi-energy spectra show two types of crossings: those related to different
Landau levels, and those associated to dynamic localization (DL), where the
electron is confined to one of the wells, despite the non-negligible tunneling
between wells. B_par couples parallel and in-plane motions producing
anti-crossings in the spectrum. However, since our approach is
non-perturbative, we are able to explore the entire frequency range. For high
frequencies, we reproduce the well known results of perfect DL given by zeroes
of a Bessel function. We find also that the system exhibits DL at the same
values of the field F, even as B_par non-zero, suggesting a hidden dynamical
symmetry in the system which we identify with different parity operations. The
return times for the electron at various values of field exhibit interesting
and complex behavior which is also studied in detail. We find that smaller
frequencies shifts the DL points to lower field F, and more importantly, yields
poorer localization by the field. We analyze the explicit time evolution of the
system, monitoring the elapsed time to return to a given well for each Landau
level, and find non-monotonic behavior for decreasing frequencies.Comment: REVTEX4 + 11 eps figs, submitted to Phys. Rev.
Mild reductions in cytosolic NADP-dependent isocitrate dehydrogenase activity result in lower amino acid contents and pigmentation without impacting growth
Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach. The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves. However, biochemical analyses revealed that the transgenic lines displayed a considerable shift in metabolism, being characterized by decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H. The plants showed little change in photosynthesis with the exception of a minor decrease in maximum photosynthetic efficiency (Fv/Fm), and a small decrease in growth compared to the wild type. These results reveal that even small changes in cytosolic NADP-dependent isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acids. These data are discussed within the context of current models for the role of the various isoforms of isocitrate dehydrogenase within plant amino acid metabolism
Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism
The MSC16 cucumber (Cucumis sativus L.) mitochondrial mutant was used to study the effect of mitochondrial dysfunction and disturbed subcellular redox state on leaf day/night carbon and nitrogen metabolism. We have shown that the mitochondrial dysfunction in MSC16 plants had no effect on photosynthetic CO2 assimilation, but the concentration of soluble carbohydrates and starch was higher in leaves of MSC16 plants. Impaired mitochondrial respiratory chain activity was associated with the perturbation of mitochondrial TCA cycle manifested, e.g., by lowered decarboxylation rate. Mitochondrial dysfunction in MSC16 plants had different influence on leaf cell metabolism under dark or light conditions. In the dark, when the main mitochondrial function is the energy production, the altered activity of TCA cycle in mutated plants was connected with the accumulation of pyruvate and TCA cycle intermediates (citrate and 2-OG). In the light, when TCA activity is needed for synthesis of carbon skeletons required as the acceptors for NH4+ assimilation, the concentration of pyruvate and TCA intermediates was tightly coupled with nitrate metabolism. Enhanced incorporation of ammonium group into amino acids structures in mutated plants has resulted in decreased concentration of organic acids and accumulation of Glu
Evaluation of heterologous in transgenic Populus tremula x P. alba plants.
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Previous issue date: 2007-01-1
Evaluation of heterologous in transgenic Populus tremula x P. alba plants.
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Previous issue date: 2007-01-10bitstream/item/178096/1/ID-27837-1.pd