Analysis of local seismic response in the historical city centre of Nafplio (Greece)

Local seismic response analysis represents a fundamental tool for assessing the seismic risk of urban areas and Cultural Heritage (CH) sites, nevertheless several open questions remain when complex geological contexts are considered. This study has been carried out in the framework of the research project STABLE (H2020 RISE-Marie Curie Action) which has the aim of evaluating the seismic action on CH sites in the Mediterranean basin. One of them being the city of Nafplio (Greece), a site characterised by a peculiar geological setting and a medium-high regional seismic hazard. Geological and geophysical data have been collected to reconstruct both the subsoil model and the seismotectonic conditions. Several seismic ambient noise measurements were carried out to establish the site resonance frequency and to validate engineering-geological cross-sections for numerical modelling of the seismic wave propagation. Combined engineering-geological, geophysical and numerical modelling allowed evaluating the local seismic response of the urban area of Nafplio, providing a zonation map with homogeneous seismic response zones for which elastic response spectra will be obtained

Novel Echocardiographic Biomarkers in the Management of Atrial Fibrillation

Purpose of Review: Atrial fibrillation (AF) is the most common arrhythmia in adults. The number of patients with AF is anticipated to increase annually, mainly due to the aging population alongside improved arrhythmia detection. AF is associated with a significantly elevated risk of hospitalization, stroke, thromboembolism, heart failure, and all-cause mortality. Echocardiography is one of the key components of routine assessment and management of AF. Therefore, the aim of this review is to briefly summarize current knowledge on “novel” echocardiographic parameters that may be of value in the management of AF patients. Recent Findings: Novel echocardiographic biomarkers and their clinical application related to the management of AF have been taken into consideration. Both standard parameters such as atrial size and volume but also novels like atrial strain and tissue Doppler techniques have been analyzed. Summary: A number of novel echocardiographic parameters have been proven to enable early detection of left atrial dysfunction along with increased diagnosis accuracy. This concerns particularly experienced echocardiographers. Hence, these techniques might improve the prediction of stroke and thromboembolic events among AF patients and need to be further developed and disseminated. Nonetheless, even the standard imaging parameters could be of significant value and should not be discontinued in everyday clinical practice. © 2019, The Author(s)

Progression From Paroxysmal to Persistent Atrial Fibrillation. Clinical Correlates and Prognosis

Objectives: We investigated clinical correlates of atrial fibrillation (AF) progression and evaluated the prognosis of patients demonstrating AF progression in a large population. Background: Progression of paroxysmal AF to more sustained forms is frequently seen. However, not all patients will progress to persistent AF. Methods: We included 1,219 patients with paroxysmal AF who participated in the Euro Heart Survey on AF and had a known rhythm status at follow-up. Patients who experienced AF progression after 1 year of follow-up were identified. Results: Progression of AF occurred in 178 (15%) patients. Multivariate analysis showed that heart failure, age, previous transient ischemic attack or stroke, chronic obstructive pulmonary disease, and hypertension were the only independent predictors of AF progression. Using the regression coefficient as a benchmark, we calculated the HATCH score. Nearly 50% of the patients with a HATCH score >5 progressed to persistent AF compared with only 6% of the patients with a HATCH score of 0. During follow-up, patients with AF progression were more often admitted to the hospital and had more major adverse cardiovascular events. Conclusions: A substantial number of patients progress to sustained AF within 1 year. The clinical outcome of these patients regarding hospital admissions and major adverse cardiovascular events was worse compared with patients demonstrating no AF progression. Factors known to cause atrial structural remodeling (age and underlying heart disease) were independent predictors of AF progression. The HATCH score may help to identify patients who are likely to progress to sustained forms of AF in the near future. \ua9 2010 American College of Cardiology Foundation

Effect of Systemic Hypertension With Versus Without Left Ventricular Hypertrophy on the Progression of Atrial Fibrillation (from the Euro Heart Survey).

Hypertension is a risk factor for both progression of atrial fibrillation (AF) and development of AF-related complications, that is major adverse cardiac and cerebrovascular events (MACCE). It is unknown whether left ventricular hypertrophy (LVH) as a consequence of hypertension is also a risk factor for both these end points. We aimed to assess this in low-risk AF patients, also assessing gender-related differences. We included 799 patients from the Euro Heart Survey with nonvalvular AF and a baseline echocardiogram. Patients with and without hypertension were included. End points after 1 year were occurrence of AF progression, that is paroxysmal AF becoming persistent and/or permanent AF, and MACCE. Echocardiographic LVH was present in 33% of 379 hypertensive patients. AF progression after 1 year occurred in 10.2% of 373 patients with rhythm follow-up. In hypertensive patients with LVH, AF progression occurred more frequently as compared with hypertensive patients without LVH (23.3% vs 8.8%, p = 0.011). In hypertensive AF patients, LVH was the most important multivariably adjusted determinant of AF progression on multivariable logistic regression (odds ratio 4.84, 95% confidence interval 1.70 to 13.78, p = 0.003). This effect was only seen in male patients (27.5% vs 5.8%, p = 0.002), while in female hypertensive patients, no differences were found in AF progression rates regarding the presence or absence of LVH (15.2% vs 15.0%, p = 0.999). No differences were seen in MACCE for hypertensive patients with and without LVH. In conclusion, in men with hypertension, LVH is associated with AF progression. This association seems to be absent in hypertensive women

International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - the quality controlled standard tool for routine identification of human and animal pathogenic fungi

Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on "DNA barcoding of human and animal pathogenic fungi." A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.This study was supported by an National Health and Medical Research Council of Australia (NH&MRC) grant [#APP1031952] to W Meyer, S Chen, V Robert, and D Ellis; CNPq [350338/2000-0] and FAPERJ [E-26/103.157/2011] grants to RM Zancope-Oliveira; CNPq [308011/2010-4] and FAPESP [2007/08575-1] Fundacao de Amparo Pesquisa do Estado de So Paulo (FAPESP) grants to AL Colombo; PEst-OE/BIA/UI4050/2014 from Fundacao para a Ciencia e Tecnologia (FCT) to C Pais; the Belgian Science Policy Office (Belspo) to BCCM/IHEM; the MEXBOL program of CONACyT-Mexico, [ref. number: 1228961 to ML Taylor and [122481] to C Toriello; the Institut Pasteur and Institut de Veil le Sanitaire to F Dromer and D Garcia-Hermoso; and the grants from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) and the Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG) to CM de Almeida Soares and JA Parente Rocha. I Arthur would like to thank G Cherian, A Higgins and the staff of the Molecular Diagnostics Laboratory, Division of Microbiology and Infectious Diseases, Path West, QEII Medial Centre. Dromer would like to thank for the technical help of the sequencing facility and specifically that of I, Diancourt, A-S Delannoy-Vieillard, J-M Thiberge (Genotyping of Pathogens and Public Health, Institut Pasteur). RM Zancope-Oliveira would like to thank the Genomic/DNA Sequencing Platform at Fundacao Oswaldo Cruz-PDTIS/FIOCRUZ [RPT01A], Brazil for the sequencing. B Robbertse and CL Schoch acknowledge support from the Intramural Research Program of the NIH, National Library of Medicine. T Sorrell's work is funded by the NH&MRC of Australia; she is a Sydney Medical School Foundation Fellow.info:eu-repo/semantics/publishedVersio

Distinctive expansion of gene families associated with plant cell wall degradation, secondary metabolism, and nutrient uptake in the genomes of grapevine trunk pathogens

BackgroundTrunk diseases threaten the longevity and productivity of grapevines in all viticulture production systems. They are caused by distantly-related fungi that form chronic wood infections. Variation in wood-decay abilities and production of phytotoxic compounds are thought to contribute to their unique disease symptoms. We recently released the draft sequences of Eutypa lata, Neofusicoccum parvum and Togninia minima, causal agents of Eutypa dieback, Botryosphaeria dieback and Esca, respectively. In this work, we first expanded genomic resources to three important trunk pathogens, Diaporthe ampelina, Diplodia seriata, and Phaeomoniella chlamydospora, causal agents of Phomopsis dieback, Botryosphaeria dieback, and Esca, respectively. Then we integrated all currently-available information into a genome-wide comparative study to identify gene families potentially associated with host colonization and disease development.ResultsThe integration of RNA-seq, comparative and ab initio approaches improved the protein-coding gene prediction in T. minima, whereas shotgun sequencing yielded nearly complete genome drafts of Dia. ampelina, Dip. seriata, and P. chlamydospora. The predicted proteomes of all sequenced trunk pathogens were annotated with a focus on functions likely associated with pathogenesis and virulence, namely (i) wood degradation, (ii) nutrient uptake, and (iii) toxin production. Specific patterns of gene family expansion were described using Computational Analysis of gene Family Evolution, which revealed lineage-specific evolution of distinct mechanisms of virulence, such as specific cell wall oxidative functions and secondary metabolic pathways in N. parvum, Dia. ampelina, and E. lata. Phylogenetically-informed principal component analysis revealed more similar repertoires of expanded functions among species that cause similar symptoms, which in some cases did not reflect phylogenetic relationships, thereby suggesting patterns of convergent evolution.ConclusionsThis study describes the repertoires of putative virulence functions in the genomes of ubiquitous grapevine trunk pathogens. Gene families with significantly faster rates of gene gain can now provide a basis for further studies of in planta gene expression, diversity by genome re-sequencing, and targeted reverse genetic approaches. The functional validation of potential virulence factors will lead to a more comprehensive understanding of the mechanisms of pathogenesis and virulence, which ultimately will enable the development of accurate diagnostic tools and effective disease management

The importance of InSAR data post-processing for the interpretation of geomorphological processes

A-DInSAR (Advanced Differential Synthetic Aperture Radar Interferometry) is widely acknowledged as one of the most powerful remote sensing tools for measuring Earth’s surface displacements over large areas, and in particular landslides. The Persistent Scatterer Interferometry (PS-InSAR or PSI) is a common A-DInSAR multitemporal technique, which allows retrieving displacement measurements with sub-centimetric precision. Characterization and interpretation of landslides can greatly benefit from the application of A-DInSAR post-processing tools, especially when extremely slow-moving phenomena are not detectable by classical geomorphological investigations, or when complex displacement patterns need to be highlighted. Detailed representations of the spatial and temporal evolution of the processes provide useful constraints during the planning stages of reconstructions and for land use purposes. The present study is part of a broader national project, focused on updating and monitoring landslide-prone slopes interacting with urban centres in the Central Apennines (Italy), by using both geomorphological and A-DInSAR analysis. Therefore, although field surveys permitted the systematic updating of the available landslide inventories, in most cases, clear indications of displacement were outlined only by the SAR interferometry results. In this regard, the preliminary results of the ongoing research focus on specific post-processing analyses of interferometric data performed in the study area. A specific PS-toolbox software, developed by NHAZCA S.r.l. as a set of post-processing plugins for the open-source software QGIS, was specifically designed to enhance spatial and temporal deformation trends of the PSI results, as well as for visualizing the differences between multi-satellite datasets. Moreover, the PS-toolbox allowed depicting subtle surface patterns within the landslide area, shedding light on kinematics and style of activity of slope instabilities. In complex morphological conditions, as the Apennines mountainous regions, the geometric distortions and the site coverage percentage can lead to a lack of information. Therefore, we compared the coverage of PSs and the accuracy of the surface velocity maps produced using different InSAR tool packages on both Sentinel-1 and COSMO-SkyMed scenes. Thus, the comparison of the resulting datasets allowed their validation in terms of measured displacements and reliability for further processing

Landslides triggered after the 16 August 2018 Mw 5.1 Molise earthquake (Italy) by a combination of intense rainfalls and seismic shaking

On 16 August 2018, a Mw 5.1 earthquake occurred in the Molise region (Central Italy) during an intense rainfall event which cumulated up to 140 mm in 3 days. Within 5 days after the seismic event, 88 landslides were surveyed and classified in disrupted and coherent as well as in first-time failures and reactivation. As it resulted by the inventorying, most of the surveyed ground effects were represented by coherent landslides involving clays, marly clays, and cover deposits on low dipping slopes. A spatial distribution analysis of landslides in relation to seismic action and rainfall intensity was carried out to evaluate the possible contribution of both the rainfall-induced saturation and the earthquake shaking to landslide triggering. Based on this analysis, in the epicentral area, it is not possible to clearly split the role of seismic and hydraulic destabilising actions, while, at greater distances, the joined contribution of rainfall and earthquake shaking could have promoted slope failures. Comparisons among collected data and existing worldwide catalogues allow to highlight, through the analysis of the spatial distribution of the surveyed landslides, the possible and not negligible effect of soil saturation during the seismic shaking both in epicentral distance vs. magnitude distribution and in number and spatial concentration of triggered ground effects

Profiling of the recent deposits of Nafplio coastal plain (Greece) from engineering geological modelling and geophysical surveys

In the current paper, some preliminary results from the research performed in the EU funded project “STABLE: Structural Stability risk assessment”, which aims to develop a methodology and tools to determine the seismic vulnerability of buildings in historical centres, are presented. The area of the historical centre of Nafplio was selected as case-study since it is one of the most important historical centers in Greece and a not negligible local seismic response may be expected based on the geological and geomorphological setting. The geological - geotechnical conditions in the study area were collected and evaluated and a geological model was identified. For this study, seismic noise measurements have been performed in the coastal plain where the town is built and processed with output resonance varying from 1 Hz up to 5 Hz, moving from the coastline towards the Acronafplia ridge. The results will be used to perform a structural stability analysis of the historical buildings using a simplified approach for specific earthquake scenarios in the future