A novel framework employing deep multi-attention channels network for the autonomous detection of metastasizing cells through fluorescence microscopy

We developed a transparent computational large-scale imaging-based framework that can distinguish between normal and metastasizing human cells. The method relies on fluorescence microscopy images showing the spatial organization of actin and vimentin filaments in normal and metastasizing single cells, using a combination of multi-attention channels network and global explainable techniques. We test a classification between normal cells (Bj primary fibroblast), and their isogenically matched, transformed and invasive counterpart (BjTertSV40TRasV12). Manual annotation is not trivial to automate due to the intricacy of the biologically relevant features. In this research, we utilized established deep learning networks and our new multi-attention channel architecture. To increase the interpretability of the network - crucial for this application area - we developed an interpretable global explainable approach correlating the weighted geometric mean of the total cell images and their local GradCam scores. The significant results from our analysis unprecedently allowed a more detailed, and biologically relevant understanding of the cytoskeletal changes that accompany oncogenic transformation of normal to invasive and metastasizing cells. We also paved the way for a possible spatial micrometre-level biomarker for future development of diagnostic tools against metastasis (spatial distribution of vimentin)

A Multilayer Interval Type-2 Fuzzy Extreme Learning Machine for the recognition of walking activities and gait events using wearable sensors

In this paper, a novel Multilayer Interval Type-2 Fuzzy Extreme Learning Machine (ML-IT2-FELM) for the recognition of walking activities and Gait events is presented. The ML-IT2-FELM uses a hierarchical learning scheme that consists of multiple layers of IT2 Fuzzy Autoencoders (FAEs), followed by a final classification layer based on an IT2-FELM architecture. The core building block in the ML-IT2-FELM is the IT2-FELM, which is a generalised model of the Interval Type-2 Radial Basis Function Neural Network (IT2-RBFNN) and that is functionally equivalent to a class of simplified IT2 Fuzzy Logic Systems (FLSs). Each FAE in the ML-IT2-FELM employs an output layer with a direct-defuzzification process based on the Nie-Tan algorithm, while the IT2-FELM classifier includes a Karnik-Mendel type-reduction method (KM). Real data was collected using three inertial measurements units attached to the thigh, shank and foot of twelve healthy participants. The validation of the ML-IT2-FELM method is performed with two different experiments. The first experiment involves the recognition of three different walking activities: Level-Ground Walking (LGW), Ramp Ascent (RA) and Ramp Descent (RD). The second experiment consists of the recognition of stance and swing phases during the gait cycle. In addition, to compare the efficiency of the ML-IT2-FELM with other ML fuzzy methodologies, a kernel-based ML-IT2-FELM that is inspired by kernel learning and called KML-IT2-FELM is also implemented. The results from the recognition of walking activities and gait events achieved an average accuracy of 99.98% and 99.84% with a decision time of 290.4ms and 105ms, respectively, by the ML-IT2-FELM, while the KML-IT2-FELM achieved an average accuracy of 99.98% and 99.93% with a decision time of 191.9ms and 94ms. The experiments demonstrate that the ML-IT2-FELM is not only an effective Fuzzy Logic-based approach in the presence of sensor noise, but also a fast extreme learning machine for the recognition of different walking activities

Using multiple feature spaces-based deep learning for tool condition monitoring in ultra-precision manufacturing

Tool condition monitoring is critical in ultra-precision manufacturing in order to optimize the performance of the overall process, while maintaining the desired part quality. Recently, Deep Learning has been successfully applied in numerous classification tasks in manufacturing, often to forecast part quality. In this paper, a novel Deep Learning data-driven modeling framework is presented, which includes fusion of multiple stacked sparse autoencoders for tool condition monitoring in ultra-precision machining. The proposed computational framework consists of two main structures. A training model that is designed with the ability to process multiple parallel feature spaces to learn the lower-level features; and a feature fusion structure that is used to learn the higher-level features and associations to tool wear. To achieve this learning structure, a modified loss function is utilized that enhances the feature extraction and classification tasks. A dataset from a real manufacturing process is used to demonstrate the performance of the proposed framework. Experimental results and simulations show that the proposed method successfully classifies the ultra-precision machining case study with over 96% accuracy, while also outperforms comparable methodologies

Methods for Rapid Pore Classification in Metal Additive Manufacturing

The additive manufacturing of metals requires optimisation to find the melting conditions that give the desired material properties. A key aspect of the optimisation is minimising the porosity that forms during the melting process. A corresponding analysis of pores of different types (e.g. lack of fusion or keyholes) is therefore desirable. Knowing that pores form under different thermal conditions allows greater insight into the optimisation process. In this work, two pore classification methods were trialled: unsupervised machine learning and defined limits. These methods were applied to 3D pore data from X-ray computed tomography and 2D pore data from micrographs. Data were collected from multiple alloys (Ti-6Al-4V, Inconel 718, Ti-5553 and Haynes 282). Machine learning was found to be the most useful for 3D pore data and defined limits for the 2D pore data; the latter worked by optimising the limits using energy densities

Experimental and numerical study of chemiluminescent species in low-pressure flames

Kathrotia T, Riedel U, Seipel A, Moshammer K, Brockhinke A. Experimental and numerical study of chemiluminescent species in low-pressure flames. APPLIED PHYSICS B. 2012;107(3):571-584