The problem of interpretation in vignette methodology in research with young people

In this paper we explore how interpretation is dealt with by researchers using a vignette methodology. Researchers using vignette methodology often struggle with interpretation: how to interpret the responses when participants shift between discussing the vignettes as themselves, taking the perspective of the character in the vignette and commenting on what ‘ought’ to happen. We argue that by foregrounding a consideration of the method with an explicitly articulated theoretical position of dialogicality, issues inherent in interpretation become a valuable addition to the research rather than an obstacle to be overcome. In the paper we discuss ‘Louise’ a young carer, detailing the various positions she takes in her talk about the vignette of Mary, a fictitious young carer, to illustrate how a perspective based in dialogical theory contributed to the analysis of her various moves through different identity positions

AFLP/SSR mapping of resistance genes to Alectra vogelii in cowpea (Vigna unguiculata l. Walp)

The parasite weed Alectra vogelii (Benth) causes significant yield reduction of cowpea in Africa. To find and map the resistance gene to A. vogelii in cowpea, a F2 population from a cross involving a resistant parent IT81D-994 and a susceptible TVX3236 was screened. Amplified fragment length polymorphism (AFLP) in combination with Single Sequence Repeat (SSR) analysis was used to identify markers that may be linked to the gene Rav3 conferring resistance to A. vogelii in the cowpea cultivar IT81D-994. The analysis of F2 individuals scored for resistance showedthat a single dominant gene conditioned A. vogelii resistance in IT81D-994 with a probability of 30 to 50%. Sixty-six AFLP/SSR markers were identified. Using MAPMAKER, four linkage groups were found. The first group showed 33 markers linked to the susceptible gene. The closest identified marker was 21.4 centimorgans away from the susceptible gene. The primer combination that showed the closest linkage was 809ACG8 (E-ACG 8 + UBC-809). The second group indicated 4 markers linked together while the third and the fourth groups showed 2 markers linkedtogether respectively. No markers were found linked to the resistance gene Rav3 conferring the resistance in the cultivated cowpea cultivar IT81D-994.La plante parasite Alectra vogelii (Benth) provoque une réduction significative du rendement de niébé en Afrique. Pour repérer et cartographier le gène de résistance à A. vogelii, une population F2 issue d’un croisement impliquant le cultivar résistant IT81D-994 et le cultivar sensible TVX3236 a été criblée. La technique Amplified fragment length polymorphism (AFLP) en combinaison avec des Séquences Simples Répétés (SSR) a été utilisée pour identifier les marqueurs qui seraient liés au gène Rav3 qui confère la résistance à A. vogelii chez le cultivar de niébé IT81D- 994. L’analyse des individus F2 montrant une résistance indique qu’un seul gène dominant conditionne la résistance à A. vogelii chez IT81D-994 avec une probabilité de 30 à 50 %. Soixante six marqueurs AFLP/SSR ont été identifiés. A l’aide de MAPMAKER, 4 groupes de liaison ont été trouvés. Le premier groupe montre 33 marqueurs liés au gène sensible. Le marqueur le plus proche identifié est à 21.4 centimorgans du gène sensible. La combinaison d’amorces ayant des liaisons les plus proches était 809ACG8 (E-ACG 8 + UBC-809). Le secondgroupe montre 4 marqueurs liés ensemble tandis que le troisième et le quatrième groupe montrent 2 marqueurs liés entre eux respectivement. Aucun marqueur n’a été trouvé lié au gène Rav3 qui confère la résistance au cultivar de niébé cultivé IT81D-994.Key words: Parasite, weeds, legumes, molecular marker

The Journey to R4D: An institutional history of an Australian Initiative on Food Security in Africa

Internal Revie

Identifying experiences of supportive care of children and young people affected by kidney failure: a qualitative systematic review.

Children and young people affected by kidney failure experience complexities in their care. Little is known about the unique needs of this young patient population group living with a long-term condition. A meta-aggregation of all qualitative studies was conducted to identify experiences of supportive care among children and young people living with kidney failure. A systematic review of qualitative studies was conducted following the Joanna Briggs Institute meta-aggregation method. This review has been reported according to the PRISMA statement guidelines. Six electronic databases (CINAHL, Cochrane Library, MEDLINE, Proquest, PsycINFO, and Scopus) were comprehensively searched by an expert systematic review librarian using keywords and subject headings, from inception to September 2022. All studies were accessed using a predetermined inclusion and exclusion criteria. Methodological quality assessment and data extraction performed. Qualitative findings accompanied by illustrative quotes from included studies were extracted and grouped into categories which created the overall synthesised findings. A total of 34 studies were included in this review representing a total of 613 children and young people affected by kidney failure. There was a total of 190 findings which created 13 categories representing experiences of supportive care. The meta-aggregation developed five synthesised findings namely: 'physical needs', 'information and technology', 'treatment and healthcare', 'social needs' and 'psychological impacts'. This systematic review identified that children and young people affected by kidney failure can experience a range of unmet supportive care needs in routine clinical services. Kidney failure impacted children and young people's self-identify, social and peer networks, introduced daily practical needs because of inherent physical and psychological burden due to the failure and associated treatments. Despite improvements in the medical management of kidney failure in children and young people, further attention is needed to optimise supported self-management in this young patient group

On the cyclically fully commutative elements of Coxeter groups

Let W be an arbitrary Coxeter group. If two elements have expressions that are cyclic shifts of each other (as words), then they are conjugate (as group elements) in W. We say that w is cyclically fully commutative (CFC) if every cyclic shift of any reduced expression for w is fully commutative (i.e., avoids long braid relations). These generalize Coxeter elements in that their reduced expressions can be described combinatorially by acyclic directed graphs, and cyclically shifting corresponds to source-to-sink conversions. In this paper, we explore the combinatorics of the CFC elements and enumerate them in all Coxeter groups. Additionally, we characterize precisely which CFC elements have the property that powers of them remain fully commutative, via the presence of a simple combinatorial feature called a band. This allows us to give necessary and sufficient conditions for a CFC element w to be logarithmic, that is, ℓ(wk)=k⋅ℓ(w) for all k≥1, for a large class of Coxeter groups that includes all affine Weyl groups and simply laced Coxeter groups. Finally, we give a simple non-CFC element that fails to be logarithmic under these conditions

Preliminary Survey of Chemical Contaminants in Water, Sediment, and Aquatic Biota at Selected Sites in Northeastern Florida Bay and Canal C-111

Several actions are under way to alter water management capabilities and practices in south Florida in order to restore a more natural hydroperiod for the Everglades. Because relatively little research has been conducted on contaminants entering Florida Bay, we undertook a preliminary study in June 1995 to determine contaminant concentrations in surface water, sediment, and biota prior to major changes in water management. The areas studied were the C-111 canal (five sites) beginning just above water control structure S-197 and extending to Manatee Bay (part of the Biscayne Bay system), Shell Creek (three sites), the mouth of Taylor River, Trout Creek (two sites), and a site near the Key Largo Ranger Station. Hydrographic observations were made at each site, and samples of water and sediment were collected at each of the five areas except the Key Largo site. Bioresidues were determined for indigenous oysters collected from the C-111 canal and Shell Creek and for transplanted oysters exposed at the Key Largo site, the C-111 canal, Shell Creek, Taylor River, and Trout Creek for 5-29 days. Water samples were analyzed for selected organochlorine pesticides and metals; sediments were analyzed for the same compounds and for polycyclic aromatic hydrocarbons (PAHs). Fish filets and shucked oysters were analyzed for selected organochlorine pesticides, polychlorinated biphenyl (PCB) congeners, and metals. Other than low dissolved oxygen at some C-111 canal sites, water quality was generally good. Most contaminants were below water and sediment quality guidelines designed to protect aquatic life, and contaminant concentrations in oysters were low. Threshold effect levels were exceeded for some organochlorine pesticides and PAHs in some sediment samples, but all values measured were below probable effect levels. Low concentrations of organochlorine pesticides were detected in sediments from the C-111 canal, Shell Creek, Taylor River, and Trout Creek; except for Taylor River, low concentrations of PCB congeners and PAHs were detected from the same areas. The only organochlorine pesticide detected in filets from seven fish was 3.2 v-g DDE/kg (wet weight) in a sea catfish. Total mercury concentrations in filets of four species of fishes ranged from 0.53 to 1.3 μg/g, wet weight, which falls within a limited consumption advisory by the State of Florida

Siglec-F is a novel intestinal M cell marker

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M-cells, demonstrating the potential to target M cells via Siglec-F