Impaired endothelial function of the retinal vasculature in hypertensive patients

<p><b>Background and Purpose:</b> Arterial hypertension constitutes a central factor in the pathogenesis of stroke. We examined endothelial function of the retinal vasculature as a model of the cerebral circulation.</p> <p><b>Methods:</b> Thirty-eight young subjects (19 hypertensive and 19 normotensive) were treated with the AT1-receptor blocker candesartan cilexetil and placebo, each over 7 days. Retinal capillary flow and blood flow velocity in the central retinal artery were assessed with scanning laser Doppler flowmetry and pulsed Doppler ultrasound, respectively. NG-monomethyl-L-arginine (L-NMMA) was infused to inhibit nitric oxide (NO) synthesis. Diffuse luminance flicker was applied to stimulate NO release.</p> <p><b>Results:</b> In normotensive subjects, L-NMMA decreased retinal capillary flow by 8.2%±13% (P<0.05) and flickering light increased mean blood flow velocity in the central retinal artery by 19%±29% (P<0.01). In contrast, no significant change to these provocative tests was seen in hypertensive subjects. Treatment with candesartan cilexetil restored a normal pattern of reactivity in retinal capillaries (L-NMMA: decrease in perfusion by 10%±17%, P<0.05) and the central retinal artery (flicker: increase in mean blood flow velocity by 42%±31%, P<0.001) in hypertensive patients.</p> <p><b>Conclusions:</b> Endothelial function of the retinal vasculature is impaired in early essential hypertension but can be improved by AT1-receptor blockade.</p&gt

Compaction control on diagenesis and reservoir quality development in red bed sandstones: a case study of Permian Rotliegend sandstones

Authigenic minerals formed during diagenesis in conjunction with compaction by burial have long been known to lead to porosity-loss of sandstones, and a subsequent deterioration in reservoir quality. The diagenetic impact on reservoir quality and permeability heterogeneity measured horizontal and vertical to bedding was characterized in three fluvio-eolian Lower Permian Rotliegend outcrops from the Flechtingen High, the northern Hesse Basin (both Germany) and the Vale of Eden (UK) using point-counting, polarized light-microscopy, helium pycnometry and permeability measurements. Results show significant porosity (10 to 35%) and permeability (0.01 to 10,000 mD) ranges largely independent of depositional environ-ment. The major control on reservoir quality in Cornberg Sandstones are dolomite and siderite cementation in conjunction with illitization and illite and kaolinite cementation, leading together with quartz cementation to a mostly cemented IGV and poorest reservoir quality (avg. horizontal permeability: 0.96 mD). Flechtingen Sandstones are most intensely compacted due to the lack of significant early diagenetic cement phases and continuous illitic grain-to-grain coatings, which inhibited intense quartz cementation but enhanced chemical compaction at quartz grain contacts, resulting in intermediate reservoir quality (avg. horizontal permeability: 34.9 mD). Penrith Sandstones lack significant authigenic phases besides quartz due to carbonate dissolution during uplift. They show the least amount of detrital feldspars and clay minerals, leading to no major reservoir quality reduction by burial diagenetic clay mineral alterations, resulting in the highest reservoir quality (avg. horizontal permeability: 5900 mD). Additional results highlight higher horizontal to vertical permeability ratios kh/kv in less homogeneous sandstones of 1000 mD of 1. Although detrital and authigenic sample compositions vary throughout the studied areas, the general effect of grain coatings coverages on syntaxial cement inhibition and chemical compaction can be delineated. This study increases the understanding of porosity reduction in sandstones, as it confirms the necessity to differentiate between the illitic grain-to-grain coatings and illitic grain-to-IGV coatings. As a result, the enhancing effect of illite on chemical compaction on quartz grain-grain boundaries can be better constrained, as well as the effect of grain coatings on quartz cementation. This is relevant for res-ervoir quality and risk assessment in hydrocarbon and geothermal plays as well as in storage

Rock typing of diagenetically induced heterogeneities – A case study from a deeply-buried clastic Rotliegend reservoir of the Northern German Basin

Reservoir quality of sandstones is mainly derived from their permeability and porosity. As a result, porosity-reducing processes need to be understood in order to evaluate and model reservoir quality in sandstones. This case study from a Rotliegend gas reservoir in the Northern German Basin utilizes petrophysical measurements in conjunction with petrography in order to assess reservoir qualities and define rock types. The most significant diagenetic factors influencing the development of the IGV (intergranular volume) are quartz cementation due to low illite grain coating coverages on grain to IGV interfaces and chemical compaction due to pronounced illite grain coating coverages on grain to grain interfaces. Where large proportions of the interface between adjacent grains are coated by illite, stronger chemical compaction (pressure dissolution) was observed to occur. This chemical compaction reduces the IGV, and thus open pore space. Permeabilities measured under decreasing confining pressures from 50 to 2 MPa were used to determine the pressure sensitivities of permeability (David et al., 1994), which ranged from 0.005 to 0.22 MPa−1. The pressure sensitivity of permeability, porosity and permeability were linked to the petrographic texture, implying three different major rock types: Type A is characterized by an uncemented petrographic texture with high porosities (avg.: 9.8%), high permeabilities (avg.: 126 mD), and low pressure sensitivities of permeability (avg.: 0.019 MPa−1). Type B is intensely cemented with reduced porosities (avg.: 4.0%), reduced permeabilities (avg.: 0.59 mD), and increased pressure sensitivities of permeability (avg.: 0.073 MPa−1). Type C is characterized by intense chemical compaction leading to the lowest porosities (avg.: 1.8%) and permeabilities (avg.: 0.037 mD) in concert with the highest pressure sensitivity of permeability (avg.: 0.12 MPa−1). The heterogeneity induced by diagenesis will have an impact on recoverable resources and flow rates in both hydrocarbon and geothermal projects in similar siliciclastic reservoirs

Inhibition of large conductance calcium-dependent potassium channel by Rho-kinase contributes to agonist-induced vasoconstriction

We tested the hypothesis that Rho-kinase inhibits the large-conductance, calcium and voltage dependent potassium (BKCa) channels thereby promoting vasoconstriction. Our results show that the Rho-kinase inhibitor, Y-27632, induced concentration-dependent relaxation in rat mesenteric artery. The selective BKCa channel inhibitors, iberiotoxin (0.1 mM) and tetraethylammonium (10 mM) increased the EC50 of Y-27632 more than 2-fold and decreased Y-27632-induced maximum relaxation (P<0.05). In the inside-out patch clamp configuration, constitutively active Rho-kinase (1mg/ml) attenuated BKCa channel activity induced by protein kinase G (PKG) (P<0.05). Y-27632 (10 mM) reversed the inhibitory effect of active Rhokinase (P<0.01). Furthermore, in the presence of Y-27632, addition of active Rho-kinase had no effect on PKG-stimulated BKCa channel activity. Taken together, our data suggest that Rho-kinase negatively regulates BKCa channels, thus providing a novel mechanism though which Rho-kinase increases smooth muscle contraction.Keywords: Rho, smooth muscle, hyperpolarization, vascular reactivity, mesenteric artery, patch clam

Modeling fracture cementation processes in calcite limestone: a phase-field study

The present work investigates the influence of crack opening rates on the development of four important calcite vein morphologies, namely fibrous, elongate-blocky, partially open, and euhedral, as a result of bitaxial growth in syntaxial veins using a multiphase-field model. The continued fracturing that occurs during synkinematic cementation in these veins is simulated using the geometric shift algorithm. The stark resemblance of the numerically sealed vein microstructures with the natural samples in terms of structural characteristics as well as remaining pore space signifies a dominant role of crack opening rates in the resulting morphological patterns. Further, simulation results of slow crack opening rates reveal that non-uniform fibers of variable lengths are formed when initial crack aperture is small, due to suppression of growth competition and vice versa

Calibrating micro-computed tomography data to permeability experiments and petrography – insights from Digital Rocks

Petrophysical measurements on core plugs integrated with petrographic information from thin-sections are established methods in reservoir quality assessment. X-ray micro-computed tomography (μCT) presents an opportunity to derive the internal structure of reservoir sandstones for digital fluid flow simulations, while si-multaneously assessing mineral distribution in 3D based on mineral densities. We compare the sin-gle-phase permeabilities obtained with fluid flow sim-ulations and experiments and discuss the anisotropic nature of the permeability tensor in both single- and two-phase flow. The results demonstrate a closer match for μCT porosity to petrophysical porosity com-pared to optical porosity, and an acceptable first order fit of the main mineralogical constituents. One-phase fluid flow simulations deliver results within 10–20 % of the laboratory measurements. Two-phase flow sim-ulations enable the assessment of relative permeabili-ties in rocks with water-sensitive minerals. However, μCT-based fluid flow simulations are computationally very demanding and time consuming due to the heter-ogeneous nature of natural sandstone samples, and require a tradeoff between resolution, representative volume, and cost. Rock composition reconstructed from μCT images can be used as a first-order approxi-mation for the composition of a sample, but is unable to confidently identify minerals that occur in minor quantities due to constraints of the chosen resolution. Thus, sandstone analyses by μCT cannot completely replace established methods

Measuring non-extensitivity parameters in a turbulent Couette-Taylor flow

We investigate probability density functions of velocity differences at different distances r measured in a Couette-Taylor flow for a range of Reynolds numbers Re. There is good agreement with the predictions of a theoretical model based on non-extensive statistical mechanics (where the entropies are non-additive for independent subsystems). We extract the scale-dependent non-extensitivity parameter q(r, Re) from the laboratory data.Comment: 8 pages, 5 figure