6,618 research outputs found
Depth differential colonization and biodiversity of mycorrhizal fungi in four prairie grass species
Non-Peer ReviewedThe biodiversity of AMF at different soil depths was studied in pure stands of the grasses crested
wheatgrass (Agropyron cristatum (L.) Gaertn.), switchgrass (Panicum virgatum L.), green
needlegrass (Nassella viridula Trin.) and western wheatgrass (Pascopyrum smithii (Rydb.) A. Löve),
growing in southwest Saskatchewan. The biodiversity of AMF was described in roots from 3 to 15,
and 30 to 45 cm depth sampled in 2006 using phylogenetic and molecular tools. Soil depth reduced
root colonization and influenced AMF community composition, which was dominated by six AMF
phylotypes of the genus Glomus. Three AMF phylotypes were common colonizers and three were
preferentially associated with some grasses. AMF communities at different depths differed from each
other in all plant stands, and diversity and richness of AMF phylotypes was higher at shallow depth,
except in N. viridula which showed higher richness of AMF in deeper root samples. We conclude
that although some AMF are general colonizers, some AMF have a strong host preference. Our
results also indicate that soil depth is a important driver of AMF phylotype distribution, and suggest
the existence of niche specialization in AMF along the soil profile, which is influenced by the host
plant
Habitats as surrogates of taxonomic and functional fish assemblages in coral reef ecosystems: a critical analysis of factors driving effectiveness
Species check-lists are helpful to establish Marine Protected Areas (MPAs) and protect local richness, endemicity, rarity, and biodiversity in general. However, such exhaustive taxonomic lists (i.e., true surrogate of biodiversity) require extensive and expensive censuses, and the use of estimator surrogates (e.g., habitats) is an appealing alternative. In truth, surrogate effectiveness appears from the literature highly variable both in marine and terrestrial ecosystems, making it difficult to provide practical recommendations for managers. Here, we evaluate how the biodiversity reference data set and its inherent bias can influence effectiveness. Specifically, we defined habitats by geomorphology, rugosity, and benthic cover and architecture criteria, and mapped them with satellite images for a New-Caledonian site. Fish taxonomic and functional lists were elaborated from Underwater Visual Censuses, stratified according to geomorphology and exposure. We then tested if MPA networks designed to maximize habitat richness, diversity and rarity could also effectively maximize fish richness, diversity, and rarity. Effectiveness appeared highly sensitive to the fish census design itself, in relation to the type of habitat map used and the scale of analysis. Spatial distribution of habitats (estimator surrogate’s distribution), quantity and location of fish census stations (target surrogate’s sampling), and random processes in the MPA design all affected effectiveness to the point that one small change in the data set could lead to opposite conclusions. We suggest that previous conclusions on surrogacy effectiveness, either positive or negative, marine or terrestrial, should be considered with caution, except in instances where very dense data sets were used without pseudo-replication. Although this does not rule out the validity of using surrogates of species lists for conservation planning, the critical joint examination of both target and estimator surrogates is needed for every case study
Human precision-cut cystic duct and gallbladder slices:a novel method for studying cholangiopathies
Background and aims: Precision-cut tissue slices (PCTS) are widely used as an ex vivo culture tissue culture technique to study pathogeneses of diseases and drug activities in organs in vitro. A novel application of the PCTS model may be in the field of translational research into cholangiopathies such as biliary atresia. Therefore, the aim of this study was to apply the precision-cut slice technique to human bile duct and gallbladder tissue.Methods: Cystic duct and gallbladder tissue derived from patients undergoing a cholecystectomy were collected, preserved and used for preparation of precision-cut cystic duct slices (PCCDS) and precision-cut gallbladder slices (PCGS). The PCCDS and PCGS were prepared using a mechanical tissue slicer and subsequently incubated for 24 and 48 h respectively in William's Medium E (WME) culture medium. Viability was assessed based on ATP/protein content and tissue morphology [hematoxylin and eosin (H&E) staining].Results: It was shown that viability, assessed by the ATP/protein content and morphology, of the PCCDS (n = 8) and PCGS (n = 8) could be maintained over the 24 and 48 h incubation period respectively. ATP/protein content of the PCCDS increased significantly from 0.58 ± 0.13 pmol/µg at 0 h to 2.4 ± 0.29 pmol/µg after 24 h incubation (P =.0003). A similar significant increase from 0.94 ± 0.22 pmol/µg at 0 h to 3.7 ± 0.41 pmol/µg after 24 h (P =.0005) and 4.2 ± 0.47 pmol/µg after 48 h (P =.0002) was observed in the PCGS. Morphological assessment of the PCCDS and PCGS showed viable tissue at 0 h and after 24 and 48 h incubation respectively.Conclusion: This study is the first to report on the use of the PCTS model for human gallbladder and cystic duct tissue. PCCDS and PCGS remain viable for an incubation period of at least 24 h, which makes them suitable for research purposes in the field of cholangiopathies, including biliary atresia.</p
Human precision-cut cystic duct and gallbladder slices:a novel method for studying cholangiopathies
Background and aims: Precision-cut tissue slices (PCTS) are widely used as an ex vivo culture tissue culture technique to study pathogeneses of diseases and drug activities in organs in vitro. A novel application of the PCTS model may be in the field of translational research into cholangiopathies such as biliary atresia. Therefore, the aim of this study was to apply the precision-cut slice technique to human bile duct and gallbladder tissue.Methods: Cystic duct and gallbladder tissue derived from patients undergoing a cholecystectomy were collected, preserved and used for preparation of precision-cut cystic duct slices (PCCDS) and precision-cut gallbladder slices (PCGS). The PCCDS and PCGS were prepared using a mechanical tissue slicer and subsequently incubated for 24 and 48 h respectively in William's Medium E (WME) culture medium. Viability was assessed based on ATP/protein content and tissue morphology [hematoxylin and eosin (H&E) staining].Results: It was shown that viability, assessed by the ATP/protein content and morphology, of the PCCDS (n = 8) and PCGS (n = 8) could be maintained over the 24 and 48 h incubation period respectively. ATP/protein content of the PCCDS increased significantly from 0.58 ± 0.13 pmol/µg at 0 h to 2.4 ± 0.29 pmol/µg after 24 h incubation (P =.0003). A similar significant increase from 0.94 ± 0.22 pmol/µg at 0 h to 3.7 ± 0.41 pmol/µg after 24 h (P =.0005) and 4.2 ± 0.47 pmol/µg after 48 h (P =.0002) was observed in the PCGS. Morphological assessment of the PCCDS and PCGS showed viable tissue at 0 h and after 24 and 48 h incubation respectively.Conclusion: This study is the first to report on the use of the PCTS model for human gallbladder and cystic duct tissue. PCCDS and PCGS remain viable for an incubation period of at least 24 h, which makes them suitable for research purposes in the field of cholangiopathies, including biliary atresia.</p
Quantum Langevin theory of excess noise
In an earlier work [P. J. Bardroff and S. Stenholm], we have derived a fully
quantum mechanical description of excess noise in strongly damped lasers. This
theory is used here to derive the corresponding quantum Langevin equations.
Taking the semi-classical limit of these we are able to regain the starting
point of Siegman's treatment of excess noise [Phys. Rev. A 39, 1253 (1989)].
Our results essentially constitute a quantum derivation of his theory and allow
some generalizations.Comment: 9 pages, 0 figures, revte
Familial X-linked mental retardation and isolated growth hormone deficiency: Clinical and molecular findings
A gene for nonspecific X-linked mental retardation (MRX41) is located in the distal segment of Xq28
Mass-radius relationships for exoplanets
For planets other than Earth, interpretation of the composition and structure
depends largely on comparing the mass and radius with the composition expected
given their distance from the parent star. The composition implies a
mass-radius relation which relies heavily on equations of state calculated from
electronic structure theory and measured experimentally on Earth. We lay out a
method for deriving and testing equations of state, and deduce mass-radius and
mass-pressure relations for key materials whose equation of state is reasonably
well established, and for differentiated Fe/rock. We find that variations in
the equation of state, such as may arise when extrapolating from low pressure
data, can have significant effects on predicted mass- radius relations, and on
planetary pressure profiles. The relations are compared with the observed
masses and radii of planets and exoplanets. Kepler-10b is apparently 'Earth-
like,' likely with a proportionately larger core than Earth's, nominally 2/3 of
the mass of the planet. CoRoT-7b is consistent with a rocky mantle over an
Fe-based core which is likely to be proportionately smaller than Earth's. GJ
1214b lies between the mass-radius curves for H2O and CH4, suggesting an 'icy'
composition with a relatively large core or a relatively large proportion of
H2O. CoRoT-2b is less dense than the hydrogen relation, which could be
explained by an anomalously high degree of heating or by higher than assumed
atmospheric opacity. HAT-P-2b is slightly denser than the mass-radius relation
for hydrogen, suggesting the presence of a significant amount of matter of
higher atomic number. CoRoT-3b lies close to the hydrogen relation. The
pressure at the center of Kepler-10b is 1.5+1.2-1.0 TPa. The central pressure
in CoRoT-7b is probably close to 0.8TPa, though may be up to 2TPa.Comment: Added more recent exoplanets. Tidied text and references. Added extra
"rock" compositions. Responded to referee comment
WiseEye: next generation expandable and programmable camera trap platform for wildlife research
Funding: The work was supported by the RCUK Digital Economy programme to the dot.rural Digital Economy Hub; award reference: EP/G066051/1. The work of S. Newey and RJI was part funded by the Scottish Government's Rural and Environment Science and Analytical Services (RESAS). Details published as an Open Source Toolkit, PLOS Journals at: http://dx.doi.org/10.1371/journal.pone.0169758Peer reviewedPublisher PD
Foot kinematics in patients with two patterns of pathological plantar hyperkeratosis
Background: The Root paradigm of foot function continues to underpin the majority of clinical foot biomechanics practice and foot orthotic therapy. There are great number of assumptions in this popular paradigm, most of which have not been thoroughly tested. One component supposes that patterns of plantar pressure and associated hyperkeratosis lesions should be associated with distinct rearfoot, mid foot, first metatarsal and hallux kinematic patterns. Our aim was to investigate the extent to which this was true.
Methods: Twenty-seven subjects with planter pathological hyperkeratosis were recruited into one of two groups.
Group 1 displayed pathological plantar hyperkeratosis only under metatarsal heads 2, 3 and 4 (n = 14). Group 2
displayed pathological plantar hyperkeratosis only under the 1st and 5th metatarsal heads (n = 13). Foot kinematics
were measured using reflective markers on the leg, heel, midfoot, first metatarsal and hallux.
Results: The kinematic data failed to identify distinct differences between these two groups of subjects, however
there were several subtle (generally <3°) differences in kinematic data between these groups. Group 1 displayed a
less everted heel, a less abducted heel and a more plantarflexed heel compared to group 2, which is contrary to
the Root paradigm.
Conclusions: There was some evidence of small differences between planter pathological hyperkeratosis groups.
Nevertheless, there was too much similarity between the kinematic data displayed in each group to classify them
as distinct foot types as the current clinical paradigm proposes
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