374 research outputs found
X-ray-provoked non-Mendelian transgenerational oncodeterminants
Cancer is the most important risk of radiation exposure. There is a definite lack of suitable test systems, human epidemiological data are only available for certain radiation types, especially not for charged particles. We use the Xiphophorus model which is genetically well characterized. As a prelude to experiments with heavy ions we report here on results obtained with x-rays to establish the necessary baseline for future studies. Apart from this direct aim we hope to obtain also a better insight in the genetical determination of cancer formation
Mitochondrial respiration and cytochrome c inhibition by sulfide in peritoneal macrophages in vitro: effects of temperature and pH
Time-dependent effects of intravenous H2S during long-term, resuscitated porcine hemorrhagic shock
Effect of intravenous H2S on porcine aortic occlusion-induced systemic inflammation and kidney ischemia/reperfusion injury
A large sample study of spin relaxation and magnetometric sensitivity of paraffin-coated Cs vapor cells
We have manufactured more than 250 nominally identical paraffin-coated Cs
vapor cells (30 mm diameter bulbs) for multi-channel atomic magnetometer
applications. We describe our dedicated cell characterization apparatus. For
each cell we have determined the intrinsic longitudinal, \sGamma{01}, and
transverse, \sGamma{02}, relaxation rates. Our best cell shows
\sGamma{01}/2\pi\approx 0.5 Hz, and \sGamma{02}/2\pi\approx 2 Hz. We find a
strong correlation of both relaxation rates which we explain in terms of
reservoir and spin exchange relaxation. For each cell we have determined the
optimal combination of rf and laser powers which yield the highest sensitivity
to magnetic field changes. Out of all produced cells, 90% are found to have
magnetometric sensitivities in the range of 9 to 30 fTHz. Noise analysis shows
that the magnetometers operated with such cells have a sensitivity close to the
fundamental photon shot noise limit
The specificity and patterns of staining in human cells and tissues of p16INK4a antibodies demonstrate variant antigen binding
The validity of the identification and classification of human cancer using antibodies to detect biomarker proteins depends upon antibody specificity. Antibodies that bind to the tumour-suppressor protein p16INK4a are widely used for cancer diagnosis and research. In this study we examined the specificity of four commercially available anti-p16INK4a antibodies in four immunological applications. The antibodies H-156 and JC8 detected the same 16 kDa protein in western blot and immunoprecipitation tests, whereas the antibody F-12 did not detect any protein in western blot analysis or capture a protein that could be recognised by the H-156 antibody. In immunocytochemistry tests, the antibodies JC8 and H-156 detected a predominately cytoplasmic localised antigen, whose signal was depleted in p16INK4a siRNA experiments. F-12, in contrast, detected a predominately nuclear located antigen and there was no noticeable reduction in this signal after siRNA knockdown. Furthermore in immunohistochemistry tests, F-12 generated a different pattern of staining compared to the JC8 and E6H4 antibodies. These results demonstrate that three out of four commercially available p16INK4a antibodies are specific to, and indicate a mainly cytoplasmic localisation for, the p16INK4a protein. The F-12 antibody, which has been widely used in previous studies, gave different results to the other antibodies and did not demonstrate specificity to human p16INK4a. This work emphasizes the importance of the validation of commercial antibodies, aside to the previously reported use, for the full verification of immunoreaction specificity
Anticipating the content and circumstances of skill transfer: Unrealistic expectations of driver training and graduated licensing?
Contrasting Effects of Sleep Restriction, Total Sleep Deprivation, and Sleep Timing on Positive and Negative Affect
Laboratory-based sleep manipulations show asymmetries between positive and negative affect, but say little about how more specific moods might change. We report extensive analyzes of items from the Positive and Negative Affect Scale (PANAS) during days following nights of chronic sleep restriction (6 h sleep opportunity), during 40 h of acute sleep deprivation under constant routine conditions, and during a week-long forced desynchrony protocol in which participants lived on a 28-h day. Living in the laboratory resulted in medium effects sizes on all positive moods (Attentiveness, General Positive Affect, Joviality, Assuredness), with a general deterioration as the days wore on. These effects were not found with negative moods. Sleep restriction reduced some positive moods, particularly Attentiveness (also General Positive), and increased Hostility. A burden of chronic sleep loss also led to lower positive moods when participants confronted the acute sleep loss challenge, and all positive moods, as well as Fearfulness, General Negative Affect and Hostility were affected. Sleeping at atypical circadian phases resulted in mood changes: all positive moods reduced, Hostility and General Negative Affect increased. Deteriorations increased the further participants slept from their typical nocturnal sleep. In most cases the changes induced by chronic or acute sleep loss or mistimed sleep waxed or waned across the waking day, with linear or various non-linear trends best fitting these time-awake-based changes. While extended laboratory stays do not emulate the fluctuating emotional demands of everyday living, these findings demonstrate that even in controlled settings mood changes systematically as sleep is shortened or mistimed
Optical Magnetometry
Some of the most sensitive methods of measuring magnetic fields utilize
interactions of resonant light with atomic vapor. Recent developments in this
vibrant field are improving magnetometers in many traditional areas such as
measurement of geomagnetic anomalies and magnetic fields in space, and are
opening the door to new ones, including, dynamical measurements of bio-magnetic
fields, detection of nuclear magnetic resonance (NMR), magnetic-resonance
imaging (MRI), inertial-rotation sensing, magnetic microscopy with cold atoms,
and tests of fundamental symmetries of Nature.Comment: 11 pages; 4 figures; submitted to Nature Physic
Adrenomedullin blockade improves catecholamine responsiveness and kidney function in resuscitated murine septic shock
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