ASSESSMENT OF NEW TECHNOLOGIES IN A MULTI-DISCIPLINARY DESIGN ANALYSIS AND OPTIMIZATION ENVIRONMENT INCLUDING RAMS AND COST DISCIPLINES

The aim of the present paper is to assess the effect of new technologies on the whole aircraft product including its costs, reliability and maintainability characteristics. Several studies have been conducted dealing with the preliminary evaluation of Reliability, Availability, Maintainability and Safety (RAMS) of conventional aircraft. They provide a very effective method to preliminary estimate RAMS characteristics but their employment is not completely suitable for the analysis of unconventional configurations adopting new technologies. This paper aims at evaluating how the aircraft costs and RAMS characteristics are affected by new structures material, natural laminar flow wing technology and unconventional actuator system (electro-hydrostatic actuators), hence an update of the state of the art models is needed. This evaluation is performed by means of a setup and execution of a Multidisciplinary Design Analysis and Optimization (MDAO) workflow. The MDAO environment includes the aircraft conceptual design, aircraft performance, structure design, engine design, on-board systems design, RAMS and maintenance cost modules. The RAMS module is used to obtain the failure rates and maintenance effort (in terms of maintenance man hour per flight hour) at subsystem level. The cost module is based on a new maintenance cost model able to estimate the operating cost of the different aircraft variants. The selected new technologies are applied to a regional jet developed within the framework of AGILE research project. For each technology, a different variant of this aircraft is analyzed. Results show that some important saves are reached both in terms of maintenance and fuel cost when new technologies are applied

Environmental & flight control system architecture optimization from a family concept design perspective

One method an Original Equipment Manufacturer (OEM) can apply to reduce development and manufacturing costs is family concept design: each product family member is designed for a different design point, but a significant amount of components is shared among the family members. In this case, a trade-off exists between member performance and commonality. In the design of complex systems, often many different architectures are possible, and the design space is too large to explore exhaustively. In this work, we present an application of a new architecture optimization method to the design of a family of passenger transport jets, with a focus on the sizing of the Environmental Control System (ECS) and Flight Control System (FCS). The architecture design space is modeled using the Architecture Design Space Graph (ADSG), a novel method for constructing model-based system architecture optimization problems. Decisions are extracted and the multi-objective optimization problem is automatically formulated. Objectives used are commonality, representing acquisition costs, and fuel burn, representing a part of operation costs. These metrics are evaluated using a cross-organizational collaborative multidisciplinary analysis toolchain, and the resulting Multidisciplinary Design Optimization (MDO) problem is solved using a multi-objective evolutionary optimization algorithm. The results show that the trade-off between commonality and fuel burn is only present above a certain commonality level

Advanced Video-Based Processing for Low-Cost Damage Assessment of Buildings under Seismic Loading in Shaking Table Tests

This paper explores the potential of a low-cost, advanced video-based technique for the assessment of structural damage to buildings caused by seismic loading. A low-cost, high-speed video camera was utilized for the motion magnification processing of footage of a two-story reinforced-concrete frame building subjected to shaking table tests. The damage after seismic loading was estimated by analyzing the dynamic behavior (i.e., modal parameters) and the structural deformations of the building in magnified videos. The results using the motion magnification procedure were compared for validation of the method of the damage assessment obtained through analyses of conventional accelerometric sensors and high-precision optical markers tracked using a passive 3D motion capture system. In addition, 3D laser scanning to obtain an accurate survey of the building geometry before and after the seismic tests was carried out. In particular, accelerometric recordings were also processed and analyzed using several stationary and nonstationary signal processing techniques with the aim of analyzing the linear behavior of the undamaged structure and the nonlinear structural behavior during damaging shaking table tests. The proposed procedure based on the analysis of magnified videos provided an accurate estimate of the main modal frequency and the damage location through the analysis of the modal shapes, which were confirmed using advanced analyses of the accelerometric data. Consequently, the main novelty of the study was the highlighting of a simple procedure with high potential for the extraction and analysis of modal parameters, with a special focus on the analysis of the modal shape's curvature, which provides accurate information on the location of the damage in a structure, while using a noncontact and low-cost method

Multidisciplinary design of a more electric regional aircraft including certification constraints

The use of electrified on-board systems is increasingly more required to reduce aircraft complexity, polluting emissions, and its life cycle cost. However, the more and all-electric aircraft configurations are still uncommon in the civil aviation context and their certifiability has yet to be proven in some aircraft segments. The aim of the present paper is to define a multidisciplinary design problem which includes some disciplines pertaining to the certification domain. In particular, the study is focused on the preliminary design of a 19 passengers small regional turboprop aircraft. Different on-board systems architectures with increasing electrification levels are considered. These architectures imply the use of bleedless technologies including electrified ice protection and environmental control systems. The use of electric actuators for secondary surfaces and landing gear are also considered. The aircraft design, which includes aerodynamic, structural, systems and propulsion domains, is then assessed by some certification disciplines. In particular, minimum performance, external noise and safety assessments are included in the workflow giving some insights on the aircraft certifiability. The results show a reduction of 3% of MTOM and 3% of fuel mass depending on the systems architecture selected. From the certification side, the design has proven to be certifiable and the margins with the certification constraint can be controlled to improve the overall design

Encrusted cystitis in an immunocompromised patient: possible coinfection by Corynebacterium urealyticum and E. coli.

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Collaborative Design of a Business Jet Family Using the AGILE 4.0 MBSE Environment

This paper presents the collaborative model-based design of a business jet family. In family design, a trade-off is made between aircraft performance, reducing fuel burn, and commonality, reducing manufacturing costs. The family is designed using Model-Based Systems Engineering (MBSE) methods developed in the AGILE 4.0 project. The EC-funded AGILE 4.0 project extends the scope of the preliminary aircraft design process to also include systems engineering phases and new design domains like manufacturing, maintenance, and certification. Stakeholders, needs, requirements, and architecture models of the business jet family are presented. Then, the collaborative Multidisciplinary Design Analysis and Optimization (MDAO) capabilities are used to integrate various aircraft design disciplines, including overall aircraft design, onboard systems design, wing structural sizing, tailplane sizing, mission analysis, and cost estimation. Decisions regarding the degree of commonality are implemented by optionally fixing the design of a shared component when sizing an aircraft

Antimicrobial activity of aztreonam in combination with old and new β-lactamase inhibitors against mbl and esbl co-producing gram-negative clinical isolates: Possible options for the treatment of complicated infections

none14noMetallo-β-lactamases (MBLs) are among the most challenging bacterial enzymes to over-come. Aztreonam (ATM) is the only β-lactam not hydrolyzed by MBLs but is often inactivated by co-produced extended-spectrum β-lactamases (ESBL). We assessed the activity of the combination of ATM with old and new β-lactamases inhibitors (BLIs) against MBL and ESBL co-producing Gram-negative clinical isolates. Six Enterobacterales and three non-fermenting bacilli co-producing MBL and ESBL determinants were selected as difficult-to-treat pathogens. ESBLs and MBLs genes were characterized by PCR and sequencing. The activity of ATM in combination with seven different BLIs (clavulanate, sulbactam, tazobactam, vaborbactam, avibactam, relebactam, zidebactam) was assessed by microdilution assay and time–kill curve. ATM plus avibactam was the most effective combination, able to restore ATM susceptibility in four out of nine tested isolates, reaching in some cases a 128-fold reduction of the MIC of ATM. In addition, relebactam and zidebactam showed to be effective, but with lesser reduction of the MIC of ATM. E. meningoseptica and C. indologenes were not inhibited by any ATM–BLI combination. ATM–BLI combinations demonstrated to be promising against MBL and ESBL co-producers, hence providing multiple options for treatment of related infections. However, no effective combination was found for some non-fermentative bacilli, suggesting the presence of additional resistance mechanisms that complicate the choice of an active therapy.openMorroni G.; Bressan R.; Fioriti S.; D'Achille G.; Mingoia M.; Cirioni O.; Di Bella S.; Piazza A.; Comandatore F.; Mauri C.; Migliavacca R.; Luzzaro F.; Principe L.; Lagatolla C.Morroni, G.; Bressan, R.; Fioriti, S.; D'Achille, G.; Mingoia, M.; Cirioni, O.; Di Bella, S.; Piazza, A.; Comandatore, F.; Mauri, C.; Migliavacca, R.; Luzzaro, F.; Principe, L.; Lagatolla, C

Multidisciplinary Design and Optimization of Regional Jet Retrofitting Activity

A retrofit analysis on a 90 passengers regional jet aircraft is performed through a multidisciplinary collaborative aircraft design and optimization highlighting the impact on costs and performance. Two different activities are accounted for selecting the best aircraft retrofit solution: a re-engining operation that allows to substitute a conventional power-plant platform with advanced geared turbofan and an on-board-systems architecture modernization, considering different levels of electrification. Besides the variables that are directly dependent from these activities, also scenario variables are considered during the optimization such as the fuel price, the fleet size and the years of utilization of the upgraded systems. The optimization is led by impacts of the retrofitting process on emissions, capital costs and saving costs, computed at industrial level. Overall aircraft design competences (aerodynamics, masses, performance, noise, and emissions) have been computed increasing the level of fidelity and reliability. The whole process is implemented in the framework of the AGILE 4.0 research project in a collaborative remote multidisciplinary approach. Results show that the engine retrofitting can be a profitable solution for both manufacturers and airliners. Conversely, the on-board-system electrification seems to be not convenient in a retrofitting process due to the high capital costs. Depending on the operative scenario, involved stakeholders can properly orient their decision on a retrofitting strategy

The role of host PrP in Transmissible Spongiform Encephalopathies

AbstractPrP has a central role in the Transmissible Spongiform Encephalopathies (TSEs), and mutations and polymorphisms in host PrP can profoundly alter the host's susceptibility to a TSE agent. However, precisely how host PrP influences the outcome of disease has not been established. To investigate this we have produced by gene targeting a series of inbred lines of transgenic mice expressing different PrP genes. This allows us to study directly the influence of the host PrP gene in TSEs. We have examined the role of glycosylation, point mutations, polymorphisms and PrP from different species on host susceptibility and the disease process both within the murine species and across species barriers

Cell Type-Specific Neuroprotective Activity of Untranslocated Prion Protein

Background: A key pathogenic role in prion diseases was proposed for a cytosolic form of the prion protein (PrP). However, it is not clear how cytosolic PrP localization influences neuronal viability, with either cytotoxic or anti-apoptotic effects reported in different studies. The cellular mechanism by which PrP is delivered to the cytosol of neurons is also debated, and either retrograde transport from the endoplasmic reticulum or inefficient translocation during biosynthesis has been proposed. We investigated cytosolic PrP biogenesis and effect on cell viability in primary neuronal cultures from different mouse brain regions. Principal Findings: Mild proteasome inhibition induced accumulation of an untranslocated form of cytosolic PrP in cortical and hippocampal cells, but not in cerebellar granules. A cyclopeptolide that interferes with the correct insertion of the PrP signal sequence into the translocon increased the amount of untranslocated PrP in cortical and hippocampal cells, and induced its synthesis in cerebellar neurons. Untranslocated PrP boosted the resistance of cortical and hippocampal neurons to apoptotic insults but had no effect on cerebellar cells. Significance: These results indicate cell type-dependent differences in the efficiency of PrP translocation, and argue that cytosolic PrP targeting might serve a physiological neuroprotective function