920 research outputs found
Effects of Exercise Training on Neuromuscular Junction Morphology and Pre- to Post-Synaptic Coupling in Young and Aged Rats
The objective of this study was to determine whether pre- to post-synaptic coupling of the neuromuscular junction (NMJ) could be maintained in the face of significant morphological remodeling brought about by exercise training, and whether aging altered this capacity. Eighteen young adult (8 mo) and eighteen aged (24 mo) Fischer 344 rats were randomly assigned to either endurance trained (treadmill running) or untrained control conditions resulting in four groups (N = 9 / group). After the 10-week intervention rats were euthanized and hindlimb muscles were surgically removed, quickly frozen at approximate resting length and stored at - 85 degrees C. The plantaris and EDL muscles were selected for study as they have different functions (ankle extensor and ankle flexor, respectively) but both are similarly and overwhelmingly comprised of fast-twitch myofibers. NMJs were stained with immunofluorescent procedures and images were collected with confocal microscopy. Each variable of interest was analyzed with a 2-way ANOVA with main effects of age and endurance training; in all cases significance was set at P \u3c = 0.05. Results showed that no main effects of aging were detected in NMJs of either the plantaris or the EDL. Similarly, endurance training failed to alter any synaptic parameters of EDL muscles. The same exercise stimulus in the plantaris however, resulted in significant pre- and post-synaptic remodeling, but without altering pre- to post-synaptic coupling of the NMJs. Myofiber profiles of the same plantaris and EDL muscles were also analyzed. Unlike NMJs, myofibers displayed significant age-related atrophy in both the plantaris and EDL muscles. Overall, these results confirm that despite significant training-induced reconfiguration of NMJs, pre- to post-synaptic coupling remains intact underscoring the importance of maintaining proper apposition of neurotransmitter release and binding sites so that effective nerve to muscle communication is assured. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved
Explanatory Supplement of the ISOGAL-DENIS Point Source Catalogue
We present version 1.0 of the ISOGAL-DENIS Point Source Catalogue (PSC),
containing more than 100,000 point sources detected at 7 and/or 15 micron in
the ISOGAL survey of the inner Galaxy with the ISOCAM instrument on board the
Infrared Space Observatory (ISO). These sources are cross-identified, wherever
possible, with near-infrared (0.8-2.2 micron) data from the DENIS survey. The
overall surface covered by the ISOGAL survey is about 16 square degrees, mostly
(95%) distributed near the Galactic plane (|b| < 1 deg), where the source
extraction can become confusion limited and perturbed by the high background
emission. Therefore, special care has been taken aimed at limiting the
photometric error to ~0.2 magnitude down to a sensitivity limit of typically 10
mJy. The present paper gives a complete description of the entries and the
information which can be found in this catalogue, as well as a detailed
discussion of the data processing and the quality checks which have been
completed. The catalogue is available via the VizieR Service at the Centre de
Donn\'ees Astronomiques de Strasbourg (CDS,
http://vizier.u-strasbg.fr/viz-bin/VizieR/) and also via the server at the
Institut d'Astrophysique de Paris (http://www-isogal.iap.fr/). A more complete
version of this paper, including a detailed description of the data processing,
is available in electronic form through the ADS service.Comment: 21 pages, 7 figures. A&A in press. Full length version with 32
figures and detailed description of the data processing is available here:
http://www-isogal.iap.fr/Publications/ExplSupplFull.ps.g
Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching
Binding, lateral diffusion and exchange are fundamental dynamic processes
involved in protein association with cellular membranes. In this study, we
developed numerical simulations of lateral diffusion and exchange of
fluorophores in membranes with arbitrary bleach geometry and exchange of the
membrane localized fluorophore with the cytosol during Fluorescence Recovery
after Photobleaching (FRAP) experiments. The model simulations were used to
design FRAP experiments with varying bleach region sizes on plasma-membrane
localized wild type GFP-Ras2 with a dual lipid anchor and mutant GFP-Ras2C318S
with a single lipid anchor in live yeast cells to investigate diffusional
mobility and the presence of any exchange processes operating in the time scale
of our experiments. Model parameters estimated using data from FRAP experiments
with a 1 micron x 1 micron bleach region-of-interest (ROI) and a 0.5 micron x
0.5 micron bleach ROI showed that GFP-Ras2, single or dual lipid modified,
diffuses as single species with no evidence of exchange with a cytoplasmic
pool. This is the first report of Ras2 mobility in yeast plasma membrane. The
methods developed in this study are generally applicable for studying diffusion
and exchange of membrane associated fluorophores using FRAP on commercial
confocal laser scanning microscopes.Comment: Accepted for publication in Physical Biology (2010). 28 pages, 7
figures, 3 table
Rotational Correlation Functions of Single Molecules
Single molecule rotational correlation functions are analyzed for several
reorientation geometries. Even for the simplest model of isotropic rotational
diffusion our findings predict non-exponential correlation functions to be
observed by polarization sensitive single molecule fluorescence microscopy.
This may have a deep impact on interpreting the results of molecular
reorientation measurements in heterogeneous environments.Comment: 5 pages, 4 figure
A method for reconstructing the variance of a 3D physical field from 2D observations: Application to turbulence in the ISM
We introduce and test an expression for calculating the variance of a
physical field in three dimensions using only information contained in the
two-dimensional projection of the field. The method is general but assumes
statistical isotropy. To test the method we apply it to numerical simulations
of hydrodynamic and magnetohydrodynamic turbulence in molecular clouds, and
demonstrate that it can recover the 3D normalised density variance with ~10%
accuracy if the assumption of isotropy is valid. We show that the assumption of
isotropy breaks down at low sonic Mach number if the turbulence is
sub-Alfvenic. Theoretical predictions suggest that the 3D density variance
should increase proportionally to the square of the Mach number of the
turbulence. Application of our method will allow this prediction to be tested
observationally and therefore constrain a large body of analytic models of star
formation that rely on it.Comment: 8 pages, 9 figures, accepted for publication in MNRA
Quantum-limited optical time transfer for future geosynchronous links
The combination of optical time transfer and optical clocks opens up the
possibility of large-scale free-space networks that connect both ground-based
optical clocks and future space-based optical clocks. Such networks promise
better tests of general relativity, dark matter searches, and gravitational
wave detection. The ability to connect optical clocks to a distant satellite
could enable space-based very long baseline interferometry (VLBI), advanced
satellite navigation, clock-based geodesy, and thousand-fold improvements in
intercontinental time dissemination. Thus far, only optical clocks have pushed
towards quantum-limited performance. In contrast, optical time transfer has not
operated at the analogous quantum limit set by the number of received photons.
Here, we demonstrate time transfer with near quantum-limited acquisition and
timing at 10,000 times lower received power than previous approaches. Over 300
km between mountaintops in Hawaii with launched powers as low as 40 W,
distant timescales are synchronized to 320 attoseconds. This nearly
quantum-limited operation is critical for long-distance free-space links where
photons are few and amplification costly -- at 4.0 mW transmit power, this
approach can support 102 dB link loss, more than sufficient for future time
transfer to geosynchronous orbits
Anisotropic Local Stress and Particle Hopping in a Deeply Supercooled Liquid
The origin of the microscopic motions that lead to stress relaxation in
deeply supercooled liquid remains unclear. We show that in such a liquid the
stress relaxation is locally anisotropic which can serve as the driving force
for the hopping of the system on its free energy surface. However, not all
hopping are equally effective in relaxing the local stress, suggesting that
diffusion can decouple from viscosity even at local level. On the other hand,
orientational relaxation is found to be always coupled to stress relaxation.Comment: 4 pages, 3 figure
Synchronization of Distant Optical Clocks at the Femtosecond Level
The use of optical clocks/oscillators in future ultra-precise navigation,
gravitational sensing, coherent arrays, and relativity experiments will require
time comparison and synchronization over terrestrial or satellite free-space
links. Here we demonstrate full unambiguous synchronization of two optical
timescales across a free-space link. The time deviation between synchronized
timescales is below 1 fs over durations from 0.1 s to 6500 s, despite
atmospheric turbulence and kilometer-scale path length variations. Over several
days, the time wander is 40 fs peak-to-peak. Our approach relies on the two-way
reciprocity of a single-spatial-mode optical link, valid to below 225
attoseconds across a turbulent 4-km path. This femtosecond level of
time-frequency transfer should enable optical networks using state-of-the-art
optical clocks/oscillators.Comment: 19 pages, 9 figure
Autonomous Bursting in a Homoclinic System
A continuous train of irregularly spaced spikes, peculiar of homoclinic
chaos, transforms into clusters of regularly spaced spikes, with quiescent
periods in between (bursting regime), by feeding back a low frequency portion
of the dynamical output. Such autonomous bursting results to be extremely
robust against noise; we provide experimental evidence of it in a CO2 laser
with feedback. The phenomen here presented display qualitative analogies with
bursting phenomena in neurons.Comment: Submitted to Phys. Rev. Lett., 14 pages, 5 figure
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