The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes

The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore–microtubule attachment in oocytes

Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing

The tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutations that underlie particular phenotypes has been a very lengthy task that many laboratories could not afford because of spatial and technical limitations. Here, we describe a simple protocol for identifying causal mutations in tomato using a mapping-by-sequencing strategy. Plants displaying phenotypes of interest are first isolated by screening an EMS mutant collection generated in the miniature cultivar Micro-Tom. A recombinant F2 population is then produced by crossing the mutant with a wild-type (WT; non-mutagenized) genotype, and F2 segregants displaying the same phenotype are subsequently pooled. Finally, whole-genome sequencing and analysis of allele distributions in the pools allow for the identification of the causal mutation. The whole process, from the isolation of the tomato mutant to the identification of the causal mutation, takes 6-12 months. This strategy overcomes many previous limitations, is simple to use and can be applied in most laboratories with limited facilities for plant culture and genotyping

Tethyan-to-boreal correlation in the Kimmeridgian using high-resolution sequence stratigraphy (Vocontian Basin, Swiss Jura, Boulonnais, Dorset)

Ammonite biostratigraphy plays a central role in the definition of Jurassic stratigraphy. Nevertheless, the strong provincialism of European ammonite species during the Kimmeridgian is a long-standing problem in correlation attempts between the boreal and Tethyan faunal realms. Moreover, the sequence-stratigraphic interpretations for northern and southern Europe given in the Jurassic chronostratigraphic chart of Hardenbol et al. in SEPM Publ. 60 (chart) (1998) are different. The present study aims to resolve this correlation problem in order to better understand the connections between the boreal and the Tethyan realms during the Kimmeridgian. A sedimentological and high-resolution sequence-stratigraphic interpretation is presented for two unpublished sections (Cras d’Hermont and Roche de Mars) in the northern Swiss Jura, where recently discovered ammonites display both boreal and Tethyan influences. Then, these sections are correlated with the same time interval in the central Swiss Jura and Vocontian Basin, which belong to the Tethyan realm. Lastly, a long-distance transect is constructed between the Vocontian Basin, Swiss Jura, northern France, and southern England, the last two areas being part of the sub-boreal realm. The main results of this work are that: (1) third-order depositional sequences, and also higher-frequency sequences, can be correlated from the Tethyan to the boreal realm; (2) the sequence-stratigraphic interpretation given by Hardenbol et al. in SEPM Publ 60 (chart) (1998) for northern Europe seems to be accurate and agrees with the sequence-stratigraphic framework established in the Swiss Jura; (3) the Late Kimmeridgian of the Swiss Jura displays boreal influences; (4) integrated high-resolution sequence-stratigraphic and cyclostratigraphic studies are a valuable approach for bridging the correlation gap between northern and southern Europe

Excursion du GFEJ 2017 dans les Charentes.

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Human c-Myc Isoforms Differentially Regulate Cell Growth and Apoptosis in Drosophila melanogaster

The human c-myc proto-oncogene, implicated in the control of many cellular processes including cell growth and apoptosis, encodes three isoforms which differ in their N-terminal region. The functions of these isoforms have never been addressed in vivo. Here, we used Drosophila melanogaster to examine their functions in a fully integrated system. First, we established that the human c-Myc protein can rescue lethal mutations of the Drosophila myc ortholog, dmyc, demonstrating the biological relevance of this model. Then, we characterized a new lethal dmyc insertion allele, which permits expression of human c-Myc in place of dMyc and used it to compare physiological activities of these isoforms in whole-organism rescue, transcription, cell growth, and apoptosis. These isoforms differ both quantitatively and qualitatively. Most remarkably, while the small c-MycS form truncated for much of its N-terminal trans-activation domain efficiently rescued viability and cell growth, it did not induce detectable programmed cell death. Our data indicate that the main functional difference between c-Myc isoforms resides in their apoptotic properties and that the N-terminal region, containing the conserved MbI motif, is decisive in governing the choice between growth and death