Glellaemic effect of Pellaea ternifolia “cuti - cuti” on Mus musculus (mouse) strain Balb / c with hyperglycemia induced with streptozotocin Peru

En el presente estudio se trabajó con 20 ejemplares machos de Mus musculus (ratón) cepa Balb/c, con peso mayor de 25 gramos, con alimentación estándar y agua ad libitum, procedentes del Bioterio del Instituto Nacional de Salud (INS) – Lima; distribuidos en grupo control (I) y experimental (II), de 10 ratones cada uno. La inducción de hiperglicemia de ambos grupos fue realizada con Estreptozotocina a dosis de 50, 60 y 70 mg/Kg (vía intraperitoneal). El grupo I recibió solución salina fisiológica y al grupo II se le administró el infuso de Cuti – cuti, por vía orogástrica. La determinación de la glicemia fue realizada en ayunas en los días 1, 4 y 7 a los 0, 60, 120, 180 y 240 minutos (durante una semana de tratamiento) con el Glucómetro marca BIONIME RightestTM GM300. Los resultados demostraron mediante un análisis de varianza (ANOVA) que la hiperglicemia fue mejor controlada a los 180 minutos y durante el cuarto día, evidenciándose en los ratones cepa Balb/c una disminución significativa de glucosa de 185 y 194.22 mg/dL respectivamente.In the present study we worked with 20 male specimens of Mus musculus (mouse) strain BALB / c, with greater weight of 25 grams, with standard food and water ad libitum, from the Bioterio the National Institute of Health (NIH) - Lima; distributed control group (I) and experimental (II) of 10 mice each. Induction of hyperglycemia of both groups was performed with streptozotocin at doses of 50, 60 and 70 mg / kg (intraperitoneally). Group I received physiological saline and the group II was given the infuso cuti - cuti, via orogastric. The determination of glycemia was performed on fasting on days 1, 4 and 7 at 0, 60, 120, 180 and 240 minutes (for a week of treatment) with glucometer brand BIONIME RightestTM GM300. The results demonstrated by analysis of variance (ANOVA) that hyperglycemia was best controlled at 180 minutes and on the fourth day, showing in mice Balb / c significantly decreased glucose of 185 and 194.22 mg / dL respectively

Use of open mobile mapping tool to assess human mobility traceability in rural offline populations with contrasting malaria dynamics

Infectious disease dynamics are affected by human mobility more powerfully than previously thought, and thus reliable traceability data are essential. In rural riverine settings, lack of infrastructure and dense tree coverage deter the implementation of cutting-edge technology to collect human mobility data. To overcome this challenge, this study proposed the use of a novel open mobile mapping tool, GeoODK. This study consists of a purposive sampling of 33 participants in six villages with contrasting patterns of malaria transmission that demonstrates a feasible approach to map human mobility. The self-reported traceability data allowed the construction of the first human mobility framework in rural riverine villages in the Peruvian Amazon. The mobility spectrum in these areas resulted in travel profiles ranging from 2 hours to 19 days; and distances between 10 to 167 km. Most Importantly, occupational-related mobility profiles with the highest displacements (in terms of time and distance) were observed in commercial, logging, and hunting activities. These data are consistent with malaria transmission studies in the area that show villages in watersheds with higher human movement are concurrently those with greater malaria risk. The approach we describe represents a potential tool to gather critical information that can facilitate malaria control activities

Unbiased Functional Proteomics Strategy for Protein Kinase Inhibitor Validation and Identification of bona fide Protein Kinase Substrates: Application to Identification of EEF1D as a Substrate for CK2

bS Supporting Informatio

Open-Source 3D Printable GPS Tracker to Characterize the Role of Human Population Movement on Malaria Epidemiology in River Networks: A Proof-of-Concept Study in the Peruvian Amazon.

Human movement affects malaria epidemiology at multiple geographical levels; however, few studies measure the role of human movement in the Amazon Region due to the challenging conditions and cost of movement tracking technologies. We developed an open-source low-cost 3D printable GPS-tracker and used this technology in a cohort study to characterize the role of human population movement in malaria epidemiology in a rural riverine village in the Peruvian Amazon. In this pilot study of 20 participants (mean age = 40 years old), 45,980 GPS coordinates were recorded over 1 month. Characteristic movement patterns were observed relative to the infection status and occupation of the participants. Applying two analytical animal movement ecology methods, utilization distributions (UDs) and integrated step selection functions (iSSF), we showed contrasting environmental selection and space use patterns according to infection status. These data suggested an important role of human movement in the epidemiology of malaria in the Peruvian Amazon due to high connectivity between villages of the same riverine network, suggesting limitations of current community-based control strategies. We additionally demonstrate the utility of this low-cost technology with movement ecology analysis to characterize human movement in resource-poor environments

A global research priority agenda to advance public health responses to fatty liver disease

Background & aims An estimated 38% of adults worldwide have non-alcoholic fatty liver disease (NAFLD). From individual impacts to widespread public health and economic consequences, the implications of this disease are profound. This study aimed to develop an aligned, prioritised fatty liver disease research agenda for the global health community. Methods Nine co-chairs drafted initial research priorities, subsequently reviewed by 40 core authors and debated during a three-day in-person meeting. Following a Delphi methodology, over two rounds, a large panel (R1 n = 344, R2 n = 288) reviewed the priorities, via Qualtrics XM, indicating agreement using a four-point Likert-scale and providing written feedback. The core group revised the draft priorities between rounds. In R2, panellists also ranked the priorities within six domains: epidemiology, models of care, treatment and care, education and awareness, patient and community perspectives, and leadership and public health policy. Results The consensus-built fatty liver disease research agenda encompasses 28 priorities. The mean percentage of ‘agree’ responses increased from 78.3 in R1 to 81.1 in R2. Five priorities received unanimous combined agreement (‘agree’ + ‘somewhat agree’); the remaining 23 priorities had >90% combined agreement. While all but one of the priorities exhibited at least a super-majority of agreement (>66.7% ‘agree’), 13 priorities had 90% combined agreement. Conclusions Adopting this multidisciplinary consensus-built research priorities agenda can deliver a step-change in addressing fatty liver disease, mitigating against its individual and societal harms and proactively altering its natural history through prevention, identification, treatment, and care. This agenda should catalyse the global health community’s efforts to advance and accelerate responses to this widespread and fast-growing public health threat. Impact and implications An estimated 38% of adults and 13% of children and adolescents worldwide have fatty liver disease, making it the most prevalent liver disease in history. Despite substantial scientific progress in the past three decades, the burden continues to grow, with an urgent need to advance understanding of how to prevent, manage, and treat the disease. Through a global consensus process, a multidisciplinary group agreed on 28 research priorities covering a broad range of themes, from disease burden, treatment, and health system responses to awareness and policy. The findings have relevance for clinical and non-clinical researchers as well as funders working on fatty liver disease and non-communicable diseases more broadly, setting out a prioritised, ranked research agenda for turning the tide on this fast-growing public health threat

Intento de suicidio en niños y adolescentes, sus características biopsicosociales y diagnósticos psiquiátricos

El presente estudio, describe las características biopsicosociales y los diagnósticos psiquiátricos en los pacientes menores de 18 años, que ingresaron al Servicio de Emergencia del Hospital Nacional Cayetano Heredia y recibieron el Diagnóstico de Intento de Suicidio. Es un estudio descriptivo y prospectivo en el que se evaluó un total de 24 pacientes, atendidos entre marzo y julio del 2002. Se utiliza el FEIA como instrumento, y las clasificaciones diagnósticas DSM-IV y CIE-10. En los resultados se encontró que el 83.3% era de sexo femenino (p < 0.01), y la media de edad fue de 15.54 años. El 79% presentaba problemas académicos; el 62.5% de los intentos ocurrió en los fines de semana y el 50% de ellos durante las mañanas (p<0.05). Los “daños reales o amenazadores” desencadenaron el 70.8% de los casos, siendo 87.5% intoxicaciones. Los síntomas más frecuentes fueron: tristeza (100%) e ideación suicida (95.8%) siendo depresión mayor (91.7%) y distimia (79.2%) los diagnósticos más frecuentes. Resalta que ocurrieron con mayor frecuencia entre pacientes de sexo femenino, los fines de semana y los “daños reales o amenazadores” estuvieron asociados a abuso físico predominando los síndromes depresivos

Intento de suicidio en niños y adolescentes: sus características biopsicosociales y diagnósticos psiquiátricos

re