1,087 research outputs found
Network formation of tissue cells via preferential attraction to elongated structures
Vascular and non-vascular cells often form an interconnected network in
vitro, similar to the early vascular bed of warm blooded embryos. Our
time-lapse recordings show that the network forms by extending sprouts, i.e.,
multicellular linear segments. To explain the emergence of such structures, we
propose a simple model of preferential attraction to stretched cells. Numerical
simulations reveal that the model evolves into a quasi-stationary pattern
containing linear segments, which interconnect above the critical volume
fraction of 0.2. In the quasi-stationary state the generation of new branches
offset the coarsening driven by surface tension. In agreement with empirical
data, the characteristic size of the resulting polygonal pattern is
density-independent within a wide range of volume fractions
Reactive oxygen species induce virus-independent MAVS-oligomerization in systemic lupus erythematosus
The increased expression of genes induced by type I interferon (IFN) is characteristic of viral infections and systemic lupus erythematosus (SLE). We showed that mitochondrial antiviral signaling (MAVS) protein, which normally forms a complex with retinoic acid gene I (RIG-I)–like helicases during viral infection, was activated by oxidative stress independently of RIG-I helicases. We found that chemically generated oxidative stress stimulated the formation of MAVS oligomers, which led to mitochondrial hyperpolarization and decreased adenosine triphosphate production and spare respiratory capacity, responses that were not observed in similarly treated cells lacking MAVS. Peripheral blood lymphocytes of SLE patients also showed spontaneous MAVS oligomerization that correlated with the increased secretion of type I IFN and mitochondrial oxidative stress. Furthermore, inhibition of mitochondrial reactive oxygen species (ROS) by the mitochondria-targeted antioxidant MitoQ prevented MAVS oligomerization and type I IFN production. ROS-dependent MAVS oligomerization and type I IFN production were reduced in cells expressing the MAVS-C79F variant, which occurs in 30% of sub-Saharan Africans and is linked with reduced type I IFN secretion and milder disease in SLE patients. Patients expressing the MAVS-C79F variant also had reduced amounts of oligomerized MAVS in their plasma compared to healthy controls. Together, our findings suggest that oxidative stress–induced MAVS oligomerization in SLE patients may contribute to the type I IFN signature that is characteristic of this syndrome
Long-Term Evolution of Massive Black Hole Binaries. III. Binary Evolution in Collisional Nuclei
[Abridged] In galactic nuclei with sufficiently short relaxation times,
binary supermassive black holes can evolve beyond their stalling radii via
continued interaction with stars. We study this "collisional" evolutionary
regime using both fully self-consistent N-body integrations and approximate
Fokker-Planck models. The N-body integrations employ particle numbers up to
0.26M and a direct-summation potential solver; close interactions involving the
binary are treated using a new implementation of the Mikkola-Aarseth chain
regularization algorithm. Even at these large values of N, two-body scattering
occurs at high enough rates in the simulations that they can not be simply
scaled to the large-N regime of real galaxies. The Fokker-Planck model is used
to bridge this gap; it includes, for the first time, binary-induced changes in
the stellar density and potential. The Fokker-Planck model is shown to
accurately reproduce the results of the N-body integrations, and is then
extended to the much larger N regime of real galaxies. Analytic expressions are
derived that accurately reproduce the time dependence of the binary semi-major
axis as predicted by the Fokker-Planck model. Gravitational wave coalescence is
shown to occur in <10 Gyr in nuclei with velocity dispersions below about 80
km/s. Formation of a core results from a competition between ejection of stars
by the binary and re-supply of depleted orbits via two-body scattering. Mass
deficits as large as ~4 times the binary mass are produced before coalescence.
After the two black holes coalesce, a Bahcall-Wolf cusp appears around the
single hole in one relaxation time, resulting in a nuclear density profile
consisting of a flat core with an inner, compact cluster, similar to what is
observed at the centers of low-luminosity spheroids.Comment: 21 page
The HR 4796A Debris System: Discovery of Extensive Exo-Ring Dust Material
The optically and IR bright, and starlight-scattering, HR 4796A ring-like
debris disk is one of the most (and best) studied exoplanetary debris systems.
The presence of a yet-undetected planet has been inferred (or suggested) from
the narrow width and inner/outer truncation radii of its r = 1.05" (77 au)
debris ring. We present new, highly sensitive, Hubble Space Telescope (HST)
visible-light images of the HR 4796A circumstellar debris system and its
environment over a very wide range of stellocentric angles from 0.32" (23 au)
to ~ 15" (1100 au). These very high contrast images were obtained with the
Space Telescope Imaging Spectrograph (STIS) using 6-roll PSF-template
subtracted coronagraphy suppressing the primary light of HR 4796A and using
three image plane occulters and simultaneously subtracting the background light
from its close angular proximity M2.5V companion. The resulting images
unambiguously reveal the debris ring embedded within a much larger,
morphologically complex, and bi-axially asymmetric exoring scattering
structure. These images at visible wavelengths are sensitive to, and map, the
spatial distribution, brightness, and radial surface density of micron size
particles over 5 dex in surface brightness. These particles in the exo-ring
environment may be unbound from the system and interacting with the local ISM.
Herein we present a new morphological and photometric view of the larger than
prior seen HR 4796A exoplanetary debris system with sensitivity to small
particles at stellocentric distances an order of magnitude greater than has
previously been observed.Comment: 28 pages, 17 figures, accepted for publication in the Astronomical
Journal 21 December 201
Excitons in InGaAs Quantum Dots without Electron Wetting Layer States
The Stranski-Krastanov (SK) growth-mode facilitates the self-assembly of
quantum dots (QDs) using lattice-mismatched semiconductors, for instance InAs
and GaAs. SK QDs are defect-free and can be embedded in heterostructures and
nano-engineered devices. InAs QDs are excellent photon emitters: QD-excitons,
electron-hole bound pairs, are exploited as emitters of high quality single
photons for quantum communication. One significant drawback of the SK-mode is
the wetting layer (WL). The WL results in a continuum rather close in energy to
the QD-confined-states. The WL-states lead to unwanted scattering and dephasing
processes of QD-excitons. Here, we report that a slight modification to the
SK-growth-protocol of InAs on GaAs -- we add a monolayer of AlAs following InAs
QD formation -- results in a radical change to the QD-excitons. Extensive
characterisation demonstrates that this additional layer eliminates the
WL-continuum for electrons enabling the creation of highly charged excitons
where up to six electrons occupy the same QD. Single QDs grown with this
protocol exhibit optical linewidths matching those of the very best SK QDs
making them an attractive alternative to standard InGaAs QDs
Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material
peer-reviewedBackground:
We aimed to determine the effect of feeding transgenic maize to sows during gestation and lactation on maternal and offspring immunity and to assess the fate of transgenic material.
Methodology/Principal Findings:
On the day of insemination, sows were assigned to one of two treatments (n = 12/treatment); 1) non-Bt control maize diet or 2) Bt-MON810 maize diet, which were fed for ~143 days throughout gestation and lactation. Immune function was assessed by leukocyte phenotyping, haematology and Cry1Ab-specific antibody presence in blood on days 0, 28 and 110 of gestation and at the end of lactation. Peripheral-blood mononuclear cell cytokine production was investigated on days 28 and 110 of gestation. Haematological analysis was performed on offspring at birth (n = 12/treatment). Presence of the cry1Ab transgene was assessed in sows' blood and faeces on day 110 of gestation and in blood and tissues of offspring at birth. Cry1Ab protein presence was assessed in sows' blood during gestation and lactation and in tissues of offspring at birth. Blood monocyte count and percentage were higher (P<0.05), while granulocyte percentage was lower (P<0.05) in Bt maize-fed sows on day 110 of gestation. Leukocyte count and granulocyte count and percentage were lower (P<0.05), while lymphocyte percentage was higher (P<0.05) in offspring of Bt maize-fed sows. Bt maize-fed sows had a lower percentage of monocytes on day 28 of lactation and of CD4+CD8+ lymphocytes on day 110 of gestation, day 28 of lactation and overall (P<0.05). Cytokine production was similar between treatments. Transgenic material or Cry1Ab-specific antibodies were not detected in sows or offspring.
Conclusions/Significance:
Treatment differences observed following feeding of Bt maize to sows did not indicate inflammation or allergy and are unlikely to be of major importance. These results provide additional data for Bt maize safety assessment.The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007–2013) under grant agreement number 211820 and the Teagasc Walsh Fellowship Programme
Mutations in the Canine CNGB3 Channel Subunit in Two Achromatopsia Models Leads to Mis-Localization of CNGA3 Expression in Retinal Outer Segments
Functional Classification of Immune Regulatory Proteins
SummaryThe members of the immunoglobulin superfamily (IgSF) control innate and adaptive immunity and are prime targets for the treatment of autoimmune diseases, infectious diseases, and malignancies. We describe a computational method, termed the Brotherhood algorithm, which utilizes intermediate sequence information to classify proteins into functionally related families. This approach identifies functional relationships within the IgSF and predicts additional receptor-ligand interactions. As a specific example, we examine the nectin/nectin-like family of cell adhesion and signaling proteins and propose receptor-ligand interactions within this family. Guided by the Brotherhood approach, we present the high-resolution structural characterization of a homophilic interaction involving the class-I MHC-restricted T-cell-associated molecule, which we now classify as a nectin-like family member. The Brotherhood algorithm is likely to have a significant impact on structural immunology by identifying those proteins and complexes for which structural characterization will be particularly informative
Polymeric alkali fullerides are stable in air
Infrared transmission, electron spin resonance, and X-ray diffraction
measurements show unambiguously that RbC and KC are stable in
air, in contrast to RbC which decomposes rapidly upon exposure.
The specimens studied transform into pure C and other byproducts when
heated above 100\dd C, approximately the temperature of the orthorhombic-fcc
phase transition. The stability of these compounds raises the possibility of
applying them as protective layers for the superconducting fullerides.Comment: Scheduled for publication in Appl. Phys. Lett. 66, 20 Feb. 1995,
typeset in REVTEX v3.0 in LaTeX. Postscript file including all figures is
available on WWW http://insti.physics.sunysb.edu/~mmartin/ under my list of
publications, or will be e-mailed by request
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