Characterization of plant growth promoting bacteria isolated from red fruits. Studies on growth promotion and fruit quality in strawberry plants

Microorganisms associated to the rizosphere of plants cultivated plants for human consumption are scarcely analyzed. However,nowadays, organic farming  where the use of microbial inoculants is essential, has arisenas emergent alternative with a great commercial interest.In this study, the characterization of the bacterial microbiotic presents in the rhizosphere of strawberry and blueberry plants has been carried out. In particular, three relevant characteristics have been determined order  to classify he isolated strains as plant growth promoting bacteria (PGPB). These characteristics are: 1) the ability to produce auxins, 2) siderophores and 3)  the solubilization of phosphates presents in the medium.On the other hand, the behavior of bacterial strains previously identified as PGPB has been studied in strawberry plants  both in greenhouse and field trials. The plants located in the greenhouse, were inoculated with the endophytic strawberries isolated strains: LTE1, LTE2, LTE3, LTE4, LTE6, EF12a, EF35 and EF113, as well as with three blueberries insolated bacteria which possess a high leves of auxin production, named AC8, ACH2 and ACH7. The strawberries plants located in the field, were inoculated with the strains Hv16, Hvs8 and Rec3.At the greenhouse trials, production parameters (precocity in flowering, number and weight of fruits) as well as biometrical parameters at the end of the trial, the fresh and dry weigth of root and shoots will be analyzed. At the field trial, the following production parameters will be evaluated: number and weight of extra-early fruit (February), number and weight of early fruit (March) and number and weight of total fruit. Quality fruit parameters as color, shape, sugar content (ºBrix), acidity, pH, vitamin C and anthocyanin content will be determined twice, once in February and again at the end of the experiment. By the time, the micro and macro-nutrient will also be analyzaed

Comprehensive transient-state study for CARMENES-NIR high thermal stability

CARMENES has been proposed as a next-generation instrument for the 3.5m Calar Alto Telescope. Its objective is finding habitable exoplanets around M dwarfs through radial velocity measurements (m/s level) in the near-infrared. Consequently, the NIR spectrograph is highly constraint regarding thermal/mechanical requirements. As a first approach, the thermal stability has been limited to \pm 0.01K (within year period) over a working temperature of 243K. This can be achieved by means of several temperature-controlled rooms. The options considered to minimise the complexity of the thermal design are here presented, as well as the transient-state thermal analyses realised to make the best choice

Body dissatisfaction in university women: new psychometric evidence from the 14-item Body Shape Questionnaire (BSQ-14)

In recent years, young university students have experienced a greater concern for the body aspect that is related to having a slim figure. In this sense, it is important to have tools to measure the dissatisfaction they may have with their body appearance. The objective of the study was to evaluate the psychometric properties of the Body Shape Questionnaire (BSQ-14). Specifically, to evaluate the internal structure of the BSQ-14, the internal consistency of the model, the evidence of discriminant validity based on the correlations of the average score of the BSQ-14 with a measure of mindfulness and the invariance of the BSQ-14 model, considering the variable of performance of physical activities. For hundred two young university students from Metropolitan Lima participated, with an average age of 20.05 years (SD = 18.18). A test battery including the BSQ-14 and Mindful Attention Awareness Scale (MASS-5) was administered. The results indicate that the one-dimensional structure of the BSQ-14 presented a good fit (x2 = 571,420; CFI = 0.962; SRMR = 0.051) and adequate reliability by the internal consistency method (ω = 0.962). Likewise, the BSQ-14 showed negative correlations with Mindfulness and body satisfaction. The BSQ-14 was shown to be invariant among university women who do or do not exercise. It is concluded that the study provides results that support the use of the BSQ-14 to measure body dissatisfaction with evidence of validity and reliability in a sample of young university students. of Metropolitan Lima

The p.(Cys150Tyr) variant in CSRP3 is associated with late-onset hypertrophic cardiomyopathy in heterozygous individuals

INTRODUCTION AND OBJECTIVES: Up to 50% of patients with hypertrophic cardiomyopathy (HCM) show no disease-causing variants in genetic studies. Mutations in CSRP3 have been associated with HCM, but evidence supporting pathogenicity is inconclusive. In this study, we describe an HCM cohort with a missense variant in CSRP3 (p.Cys150Tyr) with supporting evidence for pathogenicity and a description of the associated phenotype. METHODS: CSRP3 was sequenced in 6456 index cases with a diagnosis of HCM and in 5012 probands with other cardiomyopathies. In addition, 3372 index cases with hereditary cardiovascular disorders other than cardiomyopathies (mainly channelopathies and aortopathies) were used as controls. RESULTS: The p.(Cys150Tyr) variant was identified in 11 unrelated individuals of the 6456 HCM probands, and it was not identified in patients with other cardiomyopathies (p < 0.0001) or in our control population (p < 0.0001). Ten of the index cases were heterozygous and one was homozygous. Homozygous had a more severe phenotype. Family screening identified 17 other carriers. Wild-type individuals showed no signs of disease. The mean age at diagnosis of affected individuals was 55 ± 13 years, and the mean left ventricular wall thickness was 18 ± 3 mm. The variant showed highly age-dependent penetrance. After a mean follow-up of 11 (±8) years, no adverse events were reported in any of the HCM patients. CONCLUSIONS: The p.(Cys150Tyr) variant in CSRP3 causes late-onset and low risk form of hypertrophic cardiomyopathy in heterozygous carriers

Differential leukocyte expression of IFITM1 and IFITM3 in patients with severe pandemic influenza A(H1N1) and COVID-19

Interferon-induced transmembrane (IFITM) proteins mediate protection against enveloped viruses by blocking membrane fusion at endosomes. IFITM1 and IFITM3 are crucial for protection against influenza, and various single nucleotide polymorphisms altering their function have been linked to disease susceptibility. However, bulk IFITM1 and IFITM3 mRNA expression dynamics and their correlation with clinical outcomes have not been extensively addressed in patients with respiratory infections. In this study, we evaluated the expression of IFITM1 and IFITM3 in peripheral leukocytes from healthy controls and individuals with severe pandemic influenza A(H1N1) or coronavirus disease 2019 (COVID-19). Comparisons between participants grouped according to their clinical characteristics, underlying disease, and outcomes showed that the downregulation of IFITM1 was a distinctive characteristic of severe pandemic influenza A(H1N1) that correlated with outcomes, including mortality. Conversely, increased IFITM3 expression was a common feature of severe pandemic influenza A(H1N1) and COVID-19. Using a high-dose murine model of infection, we confirmed not only the downregulation of IFITM1 but also of IFITM3 in the lungs of mice with severe influenza, as opposed to humans. Analyses in the comparative cohort also indicate the possible participation of IFITM3 in COVID-19. Our results add to the evidence supporting a protective function of IFITM proteins against viral respiratory infections in humans.Introduction Methods - Human samples - IFITM expression in humans - Influenza infection in mice - IFITM expression in mice - Cytokine levels in mouse lungs - Study approval - Statistical analysis Results - Participant characteristics - IFITM1 and IFITM3 in patients with severe pandemic influenza A(H1N1) - High-dose influenza A (H1N1) virus infection downregulates IFITM expression in mice - IFITM1 and IFITM3 in severe COVID-19 Discussio

Hypocholesterolemia in patients with an amebic liver abscess

Background/Aims: Many parasites induce changes in the lipid profiles of the host. Cholesterol increases the virulence of Entamoeba histolytica in animal models and in vitro culture. This study aimed to determine, in patients with an amebic liver abscess, the correlation between cholesterol and other features, such as the size and number of abscesses, standard hematological and serum chemistry profiles, liver tests, and duration of hospital stay. Methods: A total of 108 patients with an amebic liver abscess and 140 clinically healthy volunteers were investigated. Cholesterol and triglycerides were measured in the sera. The data from medical observations and laboratory tests were obtained from the clinical records. Results: A total of 93% of patients with an amebic liver abscess showed hypocholesterolemia not related to any of the studied parameters. Liver function tests correlated with the size of the abscess. The most severe cases of amebic liver disease or death were found in patients whose cholesterol levels continued to decrease despite receiving antiamebic treatment and hospital care. Conclusions: Our results show that the hypocholesterolemia observed in patients with an amebic liver abscess is not related to any of the clinical and laboratory features analyzed. This is the first study relating hypocholesterolemia to severity of hepatic amebiasis

Development and evaluation of the use of a virtual health filter survey in a private primary education system in Baja California Mexico during the SARS-CoV-2 contingency (COVID-19)

Este trabajo reporta sobre el diseño, implementación y evaluación de una encuesta virtual, utilizada como filtro sanitario remoto, aplicado en el Sistema Educativo Adventista de Baja California, durante la contingencia sanitaria generada por el virus SARS-CoV-2. Esto a fin de generar información en tiempo real y de forma segura, para salvaguardar la integridad de la comunidad educativa. Donde para cumplir con los requisitos de uso, distribución, adquisición y manejo de información, se utilizaron recursos digitales gratuitos, de fácil acceso y distribución. Los cuales se adaptaron a la dinámica de comunicación del sistema educativo en cuestión. El filtro permitió adquirir información de salud de la comunidad estudiantil y facilitó la toma de decisiones respecto a la asistencia a clases por parte de los tutores, considerando las condiciones establecidas por las autoridades. A pesar de la premura en la implementación, la comunicación del sistema educativo permitió la efectiva distribución, aplicación y seguimiento del instrumento. Estrategias como estas pueden ser utilizadas para seguimiento durante la reactivación posterior a confinamientos y también analizar componentes sociales que puedan estar implicadas en estos escenarios.This work reports on the design, implementation and evaluation of a survey used as a remote sanitary filter. Which was applied in the Adventist Educational System of Baja California at the beginning of the SARS-CoV-2 sanitary contingency with the intention of acquiring information safely and in real time. Allowing to safeguard the integrity of the educational community as a whole. Accessible and easily distributed digital resources were used to meet the necessary requirements of distribution, acquisition and management of the information. These were adapted to the previously established communication dynamic of the educational system. The use of the survey as a sanitary filter allowed to acquire relevant health information from the student community and facilitated decision-making regarding class attendance by their tutors. These in consideration to the conditions established by educational and health authorities. Despite the short time during the design and implementation, the communication of the educational system allowed the effective distribution, application and monitoring of the digital survey. Similar strategies can be used in the follow-up during the reactivation after lockdown and to analyze social components that may be involved in these scenarios.Facultad de Informátic