1,002 research outputs found
A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the <em>Saccharomyces cerevisiae</em> Elongator complex
Elongator is a conserved, multi-protein complex discovered in Saccharomyces cerevisiae, loss of which confers a range of pleiotropic phenotypes. Elongator in higher eukaryotes is required for normal growth and development and a mutation in the largest subunit of human Elongator (Elp1) causes familial dysautonomia, a severe recessive neuropathy. Elongator promotes addition of mcm(5) and ncm(5) modifications to uridine in the tRNA anticodon ‘wobble’ position in both yeast and higher eukaryotes. Since these modifications are required for the tRNAs to function efficiently, a translation defect caused by hypomodified tRNAs may therefore underlie the variety of phenotypes associated with Elongator dysfunction. The Elp1 carboxy-terminal domain contains a highly conserved arginine/lysine-rich region that resembles a nuclear localization sequence (NLS). Using alanine substitution mutagenesis, we show that this region is essential for Elongator's function in tRNA wobble uridine modification. However, rather than acting to determine the nucleo-cytoplasmic distribution of Elongator, we find that the basic region plays a critical role in a novel interaction between tRNA and the Elp1 carboxy-terminal domain. Thus the conserved basic region in Elp1 may be essential for tRNA wobble uridine modification by acting as tRNA binding motif
The validity and reliability of the exposure index as a metric for estimating the radiation dose to the patient
Introduction
With the introduction of digital radiography, the feedback between image quality and over-exposure has been partly lost which in some cases has led to a steady increase in dose. Over the years the introduction of exposure index (EI) has been used to resolve this phenomenon referred to as ‘dose creep’. Even though EI is often vendor specific it is always a related of the radiation exposure to the detector. Due to the nature of this relationship EI can also be used as a patient dose indicator, however this is not widely investigated in literature.
Methods
A total of 420 dose-area-product (DAP) and EI measurements were taken whilst varying kVp, mAs and body habitus on two different anthropomorphic phantoms (pelvis and chest). Using linear regression, the correlation between EI and DAP were examined. Additionally, two separate region of interest (ROI) placements/per phantom where examined in order to research any effect on EI.
Results
When dividing the data into subsets, a strong correlation between EI and DAP was shown with all R-squared values > 0.987. Comparison between the ROI placements showed a significant difference between EIs for both placements.
Conclusion
This research shows a clear relationship between EI and radiation dose which is dependent on a wide variety of factors such as ROI placement, body habitus. In addition, pathology and manufacturer specific EI’s are likely to be of influence as well.
Implications for practice
The combination of DAP and EI might be used as a patient dose indicator. However, the influencing factors as mentioned in the conclusion should be considered and examined before implementation
Theortetical Models of Extrasolar Giant Planets
The recent discoveries of giant planets around nearby stars have galvanized
the planetary science community, astronomers, and the public at large. Since
{\it direct} detection is now feasible, and is suggested by the recent
acquisition of Gl229 B, it is crucial for the future of extrasolar planet
searches that the fluxes, evolution, and physical structure of objects from
Saturn's mass to 15 Juipter masses be theoretically investigated. We discuss
our first attempts to explore the characteristics of extrasolar giant planets
(EGPs), in aid of both NASA's and ESA's recent plans to search for such planets
around nearby stars.Comment: LaTeX, using espcrc2.sty style files from Elsevier, 10 pages, 4
figures, to be published in the Proceedings of the International Conference
on "Sources and Detection of Dark Matter in the Universe," ed. by D. Sanders
et al. (Nuclear Physics B Supplement), 199
Recommended Medical and Non-Medical Factors to Assess Military Preventable Deaths: Subject Matter Experts Provide Valuable Insights
INTRODUCTION: Historically, there has been variability in the methods for determining preventable death within the US Department of Defense. Differences in methodologies partially explain variable preventable death rates ranging from 3% to 51%. The lack of standard review process likely misses opportunities for improvement in combat casualty care. This project identified recommended medical and non-medical factors necessary to (1) establish a comprehensive preventable death review process and (2) identify opportunities for improvement throughout the entire continuum of care.
METHODS: This qualitative study used a modified rapid assessment process that includes the following steps: (1) identification and recruitment of US government subject matter experts (SMEs); (2) multiple cycles of data collection via key informant interviews and focus groups; (3) consolidation of information collected in these interviews; and (4) iterative analysis of data collected from interviews into common themes. Common themes identified from SME feedback were grouped into the following subject areas: (1) prehospital, (2) in-hospital and (3) forensic pathology.
RESULTS: Medical recommendations for military preventable death reviews included the development, training, documentation, collection, analysis and reporting of the implementation of the Tactical Combat Casualty Care Guidelines, Joint Trauma System Clinical Practice Guidelines and National Association of Medical Examiners autopsy standards. Non-medical recommendations included training, improved documentation, data collection and analysis of non-medical factors needed to understand how these factors impact optimal medical care.
CONCLUSIONS: In the operational environment, medical care must be considered in the context of non-medical factors. For a comprehensive preventable death review process to be sustainable in the military health system, the process must be based on an appropriate conceptual framework implemented consistently across all military services
Tevatron Beam Halo Collimation System: Design, Operational Experience and New Methods
Collimation of proton and antiproton beams in the Tevatron collider is
required to protect CDF and D0 detectors and minimize their background rates,
to keep irradiation of superconducting magnets under control, to maintain
long-term operational reliability, and to reduce the impact of beam-induced
radiation on the environment. In this article we briefly describe the design,
practical implementation and performance of the collider collimation system,
methods to control transverse and longitudinal beam halo and two novel
collimation techniques tested in the Tevatron.Comment: 25 p
Patchiness and Demographic Noise in Three Ecological Examples
Understanding the causes and effects of spatial aggregation is one of the
most fundamental problems in ecology. Aggregation is an emergent phenomenon
arising from the interactions between the individuals of the population, able
to sense only -at most- local densities of their cohorts. Thus, taking into
account the individual-level interactions and fluctuations is essential to
reach a correct description of the population. Classic deterministic equations
are suitable to describe some aspects of the population, but leave out features
related to the stochasticity inherent to the discreteness of the individuals.
Stochastic equations for the population do account for these
fluctuation-generated effects by means of demographic noise terms but, owing to
their complexity, they can be difficult (or, at times, impossible) to deal
with. Even when they can be written in a simple form, they are still difficult
to numerically integrate due to the presence of the "square-root" intrinsic
noise. In this paper, we discuss a simple way to add the effect of demographic
stochasticity to three classic, deterministic ecological examples where
aggregation plays an important role. We study the resulting equations using a
recently-introduced integration scheme especially devised to integrate
numerically stochastic equations with demographic noise. Aimed at scrutinizing
the ability of these stochastic examples to show aggregation, we find that the
three systems not only show patchy configurations, but also undergo a phase
transition belonging to the directed percolation universality class.Comment: 20 pages, 5 figures. To appear in J. Stat. Phy
Antihydrogen formation dynamics in a multipolar neutral anti-atom trap
Antihydrogen production in a neutral atom trap formed by an octupole-based
magnetic field minimum is demonstrated using field-ionization of weakly bound
anti-atoms. Using our unique annihilation imaging detector, we correlate
antihydrogen detection by imaging and by field-ionization for the first time.
We further establish how field-ionization causes radial redistribution of the
antiprotons during antihydrogen formation and use this effect for the first
simultaneous measurements of strongly and weakly bound antihydrogen atoms.
Distinguishing between these provides critical information needed in the
process of optimizing for trappable antihydrogen. These observations are of
crucial importance to the ultimate goal of performing CPT tests involving
antihydrogen, which likely depends upon trapping the anti-atom
In search of hair damage using metabolomics?
YesHair fibres are extraordinary materials, not least because they are exquisitely formed by each of the 5 million or so hair follicles on our bodies and have functions that cross from physiology to psychology, but also because they have well known resistance to degradation as seen in hair surviving from archaeological and historical samples [1]. Hair fibres on the head grow at around 1cm each month, together totalling approximately 12km of growth per person per year. Each fibre is incredibly strong for its small diameter; with one fibre typically holding 100g and together a well-formed ponytail [allegedly] has the collective strength to support the weight of a small elephant! Hair – and from here I mean scalp hair – is under constant scrutiny by each of us; whether it be style, split ends, the first few grey hairs or the collection of hairs in the shower that should be firmly attached - leading to the fear that is hair loss
Search For Trapped Antihydrogen
We present the results of an experiment to search for trapped antihydrogen
atoms with the ALPHA antihydrogen trap at the CERN Antiproton Decelerator.
Sensitive diagnostics of the temperatures, sizes, and densities of the trapped
antiproton and positron plasmas have been developed, which in turn permitted
development of techniques to precisely and reproducibly control the initial
experimental parameters. The use of a position-sensitive annihilation vertex
detector, together with the capability of controllably quenching the
superconducting magnetic minimum trap, enabled us to carry out a
high-sensitivity and low-background search for trapped synthesised antihydrogen
atoms. We aim to identify the annihilations of antihydrogen atoms held for at
least 130 ms in the trap before being released over ~30 ms. After a three-week
experimental run in 2009 involving mixing of 10^7 antiprotons with 1.3 10^9
positrons to produce 6 10^5 antihydrogen atoms, we have identified six
antiproton annihilation events that are consistent with the release of trapped
antihydrogen. The cosmic ray background, estimated to contribute 0.14 counts,
is incompatible with this observation at a significance of 5.6 sigma. Extensive
simulations predict that an alternative source of annihilations, the escape of
mirror-trapped antiprotons, is highly unlikely, though this possibility has not
yet been ruled out experimentally.Comment: 12 pages, 7 figure
Spin fluctuations in nearly magnetic metals from ab-initio dynamical spin susceptibility calculations:application to Pd and Cr95V5
We describe our theoretical formalism and computational scheme for making
ab-initio calculations of the dynamic paramagnetic spin susceptibilities of
metals and alloys at finite temperatures. Its basis is Time-Dependent Density
Functional Theory within an electronic multiple scattering, imaginary time
Green function formalism. Results receive a natural interpretation in terms of
overdamped oscillator systems making them suitable for incorporation into spin
fluctuation theories. For illustration we apply our method to the nearly
ferromagnetic metal Pd and the nearly antiferromagnetic chromium alloy Cr95V5.
We compare and contrast the spin dynamics of these two metals and in each case
identify those fluctuations with relaxation times much longer than typical
electronic `hopping times'Comment: 21 pages, 9 figures. To appear in Physical Review B (July 2000
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