Compellingly high SARS-CoV-2 susceptibility of Golden Syrian hamsters suggests multiple zoonotic infections of pet hamsters during the COVID-19 pandemic

Golden Syrian hamsters (Mesocricetus auratus) are used as a research model for severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Millions of Golden Syrian hamsters are also kept as pets in close contact to humans. To determine the minimum infective dose (MID) for assessing the zoonotic transmission risk, and to define the optimal infection dose for experimental studies, we orotracheally inoculated hamsters with SARS-CoV-2 doses from 1 * 105 to 1 * 10-4 tissue culture infectious dose 50 (TCID50). Body weight and virus shedding were monitored daily. 1 * 10-3 TCID50 was defined as the MID, and this was still sufficient to induce virus shedding at levels up to 102.75 TCID50/ml, equaling the estimated MID for humans. Virological and histological data revealed 1 * 102 TCID50 as the optimal dose for experimental infections. This compelling high susceptibility leading to productive infections in Golden Syrian hamsters must be considered as a potential source of SARS-CoV-2 infection for humans that come into close contact with pet hamsters

Stability and binding affinity of DNA/chitosan complexes by polyanion competition

The stability of DNA/chitosan complexes upon exposure to hyaluronic acid, chondroitin sulfate, and heparin, was assessed by fluorescence spectroscopy to quantify DNA release. Only the highly charged heparin was found to release DNA from the complexes. Complex stability upon exposure to heparin increased with the degree of deacetylation and molecular weight of chitosan and with the ratio of chitosan amino groups to DNA phosphate groups (N/P ratio) in the complexes. Isothermal titration microcalorimetry revealed that among polyanions tested, only heparin has a binding affinity to chitosan approaching that of DNA and can therefore release DNA from the complexes. These results also indicate that anionic components with sufficiently high charge density can induce extracellular or intracellular release of DNA, the former negatively affecting delivery efficiency while the latter is required for gene transfer to occur. Our findings also suggest that increased N/P ratio of the complexes can play an important role in preventing premature dissociation of DNA/polycation complexes upon interaction with anionic components in extracellular milieu. (C) 2017 Elsevier Ltd. All rights reserved.Peer reviewe

Understanding the Random Displacement Model: From Ground-State Properties to Localization

We give a detailed survey of results obtained in the most recent half decade which led to a deeper understanding of the random displacement model, a model of a random Schr\"odinger operator which describes the quantum mechanics of an electron in a structurally disordered medium. These results started by identifying configurations which characterize minimal energy, then led to Lifshitz tail bounds on the integrated density of states as well as a Wegner estimate near the spectral minimum, which ultimately resulted in a proof of spectral and dynamical localization at low energy for the multi-dimensional random displacement model.Comment: 31 pages, 7 figures, final version, to appear in Proceedings of "Spectral Days 2010", Santiago, Chile, September 20-24, 201

Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain

The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast is attracted during cytokinesis. Proteins have been discovered that are part of the molecular memory but little is known about how they contribute to phragmoplast guidance. Previously, we found that the microtubule-associated protein AIR9 is found in the cortical division zone at preprophase and returns during cell plate insertion but is absent from the cortex during the intervening mitosis. To identify new components of the preprophase memory, we searched for proteins that interact with AIR9. We detected the kinesin-like calmodulin-binding protein, KCBP, which can be visualized at the predicted cortical site throughout division. A truncation study of KCBP indicates that its MyTH4-FERM domain is required for linking the motor domain to the cortex. These results suggest a mechanism by which minus-end-directed KCBP helps guide the centrifugally expanding phragmoplast to the cortical division site.Instituto de Biotecnologia y Biologia Molecula

Ground-based remote sensing of aerosol properties using high-resolution infrared emission and lidar observations in the High Arctic

Arctic amplification, the phenomenon that the Arctic is warming faster than the global mean, is still not fully understood. The Transregional Collaborative Research Centre “TRR 172: ArctiC Amplification: Climate Relevant Atmospheric and SurfaCe Processes, and Feedback Mechanisms (AC)3” program, funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), contributes towards this research topic. For the purpose of measuring aerosol components, a Fourier transform infrared spectrometer (FTIR), for measuring downwelling emission (in operation since 2019), and a Raman lidar are operated at the joint Alfred Wegener Institute for Polar and Marine Research and Paul Emile Victor Institute (AWIPEV) research base in Ny-Ålesund, Spitsbergen (79∘ N, 12∘ E). To carry out aerosol retrieval using measurements from the FTS, the LBLDIS retrieval algorithm, based on a combination of the Line-by-Line Radiative Transfer Model (LBLRTM) and the DIScrete Ordinate Radiative Transfer (DISORT) algorithm, is modified for different aerosol types (dust, sea salt, black carbon, and sulfate), aerosol optical depth (AOD), and effective radius (Reff). Using lidar measurement, an aerosol and cloud classification method is developed to provide basic information about the distribution of aerosols or clouds in the atmosphere and is used as an indicator to perform aerosol or cloud retrievals with the FTS. Therefore, a two-instrument joint-observation scheme is designed and subsequently used on the data measured from 2019 to the present. In order to introduce this measurement technique in detail, an aerosol-only case study is presented using data from 10 June 2020. In the aerosol-only case, the retrieval results show that sulfate is the dominant aerosol throughout the day (τ900cm-1 = 0.007 ± 0.0027), followed by dust (τ900cm-1 = 0.0039 ± 0.0029) and black carbon (τ900cm-1 = 0.0017 ± 0.0007). Sea salt (τ900cm-1 = 0.0012 ± 0.0002), which has the weakest emission ability in the infrared wave band, shows the lowest AOD value. Such proportions of sulfate, dust, and BC also show good agreement with Modern-Era Retrospective analysis for Research and Applications version 2 (MERRA-2) reanalysis data. Additionally, comparison with a Sun photometer (AErosol RObotic NETwork – AERONET) shows the daily variation in the AOD retrieved from FTS to be similar to that retrieved by Sun photometer. Using this method, long-term observations (from April to August 2020) are retrieved and presented. We find that sulfate is often present in the Arctic; it is higher in spring and lower in summer. Similarly, BC is also frequently observed in the Arctic, with less obvious seasonal variation than sulfate. A BC outburst event is observed each spring and summer. In spring, sulfate and BC are dominant, whereas sea salt and dust are relatively low. In addition, a sea salt enhancement event is observed in summertime, which might be due to the melting of sea ice and emissions from nearby open water. From the retrieved results over a long time period, no clear correlations are found; thus, the aforementioned species can be retrieved independently of one another.</p

Measurement of the W-pair Production Cross-section and W Branching Ratios at s\sqrt{s}=205 and 207 GeV

The cross-section for the process e+e-->W+W- was measured with the data sample collected by DELPHI at centre-of-mass energies up to 209 GeV and corresponding to a total integrated luminosity of about 209 pb^-1. The branching ratios of the W decay were also measured; from them the value of |Vcs| was extracted. The results are compared with the most recent calculations in the frame of the Standard Model

Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism

The majority of bovine spongiform encephalopathy (BSE) cases have been ascribed to the classical form of the disease. H-type and L-type BSE cases have atypical molecular profiles compared to classical BSE and are thought to arise spontaneously. However, one case of H-type BSE was associated with a heritable E211K mutation in the prion protein gene. The purpose of this study was to describe transmission of this unique isolate of H-type BSE when inoculated into a calf of the same genotype by the intracranial route. Electroretinograms were used to demonstrate preclinical deficits in retinal function, and optical coherence tomography was used to demonstrate an antemortem decrease in retinal thickness. The calf rapidly progressed to clinical disease (9.4 months) and was necropsied. Widespread distribution of abnormal prion protein was demonstrated within neural tissues by western blot and immunohistochemistry. While this isolate is categorized as BSE-H due to a higher molecular mass of the unglycosylated PrPSc isoform, a strong labeling of all 3 PrPSc bands with monoclonal antibodies 6H4 and P4, and a second unglycosylated band at approximately 14 kDa when developed with antibodies that bind in the C-terminal region, it is unique from other described cases of BSE-H because of an additional band 23 kDa demonstrated on western blots of the cerebellum. This work demonstrates that this isolate is transmissible, has a BSE-H phenotype when transmitted to cattle with the K211 polymorphism, and has molecular features that distinguish it from other cases of BSE-H described in the literature

3D morphometric analysis of calcified cartilage properties using micro-computed tomography

Objective: Our aim is to establish methods for quantifying morphometric properties of calcified cartilage (CC) from micro-computed tomography (mu CT). Furthermore, we evaluated the feasibility of these methods in investigating relationships between osteoarthritis (OA), tidemark surface morphology and open subchondral channels (OSCCs). Method: Samples (n = 15) used in this study were harvested from human lateral tibial plateau (n = 8). Conventional roughness and parameters assessing local 3-dimensional (3D) surface variations were used to quantify the surface morphology of the CC. Subchondral channel properties (percentage, density, size) were also calculated. As a reference, histological sections were evaluated using Histopathological osteoarthritis grading (OARSI) and thickness of CC and subchondral bone (SCB) was quantified. Results: OARSI grade correlated with a decrease in local 3D variations of the tidemark surface (amount of different surface patterns (r(s) = -0.600, P = 0.018), entropy of patterns (EP) (r(s) = -0.648, P = 0.018), homogeneity index (HI) (r(s) = 0.555, P = 0.032)) and tidemark roughness (TMR) (r(s) = -0.579, P = 0.024). Amount of different patterns (ADP) and EP associated with channel area fraction (CAF) (r(p) = 0.876, P <0.0001; r(p) = 0.665, P = 0.007, respectively) and channel density (CD) (r(p) = 0.680, P = 0.011; r(p) = 0.582, P = 0.023, respectively). TMR was associated with CAF (r(p) = 0.926, P <0.0001) and average channel size (r(p) = 0.574, P = 0.025). CC topography differed statistically significantly in early OA vs healthy samples. Conclusion: We introduced a mu-CT image method to quantify 3D CC topography and perforations through CC. CC topography was associated with OARSI grade and OSCC properties; this suggests that the established methods can detect topographical changes in tidemark and CC perforations associated with OA. (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe