Computation of steady and unsteady quasi-one-dimensional viscous/inviscid interacting internal flows at subsonic, transonic, and supersonic Mach numbers

Computations of viscous-inviscid interacting internal flowfields are presented for steady and unsteady quasi-one-dimensional (Q1D) test cases. The unsteady Q1D Euler equations are coupled with integral boundary-layer equations for unsteady, two-dimensional (planar or axisymmetric), turbulent flow over impermeable, adiabatic walls. The coupling methodology differs from that used in most techniques reported previously in that the above mentioned equation sets are written as a complete system and solved simultaneously; that is, the coupling is carried out directly through the equations as opposed to coupling the solutions of the different equation sets. Solutions to the coupled system of equations are obtained using both explicit and implicit numerical schemes for steady subsonic, steady transonic, and both steady and unsteady supersonic internal flowfields. Computed solutions are compared with measurements as well as Navier-Stokes and inverse boundary-layer methods. An analysis of the eigenvalues of the coefficient matrix associated with the quasi-linear form of the coupled system of equations indicates the presence of complex eigenvalues for certain flow conditions. It is concluded that although reasonable solutions can be obtained numerically, these complex eigenvalues contribute to the overall difficulty in obtaining numerical solutions to the coupled system of equations

An insulin-like growth factor (IGF) binding protein enhances the biologic response to IGF-I.

The insulin-like growth factors IGF-I and IGF-II circulate in blood bound to carrier proteins. The higher molecular mass IGF-binding protein complex (150 kDa) is composed of subunits, and one subunit that forms this complex is growth hormone dependent. In addition, many cell types and tissues secrete another form of IGF binding protein that is not growth hormone dependent. Both forms of the IGF binding protein are believed to inactivate the IGFs and to function as delivery systems to tissues. This conclusion was based on studies that determined the effects of impure preparations of these binding proteins or that examined the effect of these proteins only on the insulin-like actions of the IGFs. We report here that a pure preparation of the extracellular form of the IGF binding protein (purified from human amniotic fluid) markedly potentiated replication of several cell types in response to human IGF-I. Secondary cultures of human, mouse, and chicken embryo fibroblasts as well as porcine aortic smooth muscle cells showed marked enhancement of their DNA synthesis response (2.8- to 4.4-fold increases) to IGF-I in the presence of this protein. These responses were synergistic since the sum of the responses to either IGF-I or to the binding protein alone was between 8 and 17% of the increase obtained in cultures exposed to both peptides. The binding protein not only potentiated the DNA synthesis response but also enhanced the increase in cell number in response to IGF-I. This stimulation is specific for growth factors that bind to the binding protein since incubation with insulin, which binds to the type I IGF receptor but not to the binding protein, did not result in potentiation of this response. We conclude that a form of IGF binding protein that is present in extracellular fluids and is secreted by many types of cells can markedly potentiate the cellular response to IGF-I

Disturbance and Predictability of Flowering Patterns in Bird-Pollinated Cloud Forest Plants

The distribution and flowering patterns of hummingbird—pollinated plants were compared from July 1981 to June 1983 in three patch types in cloud forest at Monteverde, Costa Rica. Study plots were: (1) four recent, large (1100—2500 m2) disturbances ("cutovers") produced by cutting vegetation, (2) six recent, smaller (200—600 m2) disturbances caused by treefalls, and (3) four plots (1600—1800 m2) of canopied forest. Based on published literature dealing with communities that characterize different regimes of disturbance, we tested one assumption and two hypotheses. Assumption: Plant species composition differs among the three patch types. Hypothesis 1: Phenotypic specialization by plants for co—evolved interactions with hummingbirds will be lowest in large gaps, highest in forest, and intermediate in treefalls. Hypothesis 2: Predictability of flowering phenologies and nectar production will be lowest in large gaps, highest in forest, intermediate in treefalls. Neither the assumption nor the hypotheses were supported by the results. The patch mosaic in this cloud forest was not associated with major differences in species composition of bird—pollinated plants. Most species studied were self—compatible. Most abundant in cutovers were species with long corollas, relatively specialized for attracting long—billed hummingbirds. Species with short corollas, which can be visited by many hummingbird species and some insects, were most abundant in treefalls and forest. Variation in phenological patterns showed no consistent trends among patch types. Predictability of flower and nectar production tended to be greatest in treefalls, which are foci of concentrated flowering activity by all species. Discrepancies between our results and previous studies can be ascribed to two facts. (1) Much of the literature dealing with ecological consequences of disturbance has dealt with large—scale anthropogenic disturbances such as old fields of the eastern USA, whereas we studied small, natural, or quasi—natural disturbances. (2) Studies of forest disturbance have focused on the tree layer, whereas we studied the understory herbs, shrubs, and epiphytes. Natural disturbance usually involves death and replacement of one or more trees, whereas individuals of other life forms may persist through the disturbance

Purification of a 31,000-dalton insulin-like growth factor binding protein from human amniotic fluid. Isolation of two forms with different biologic actions.

Human amniotic fluid has been shown to contain a protein that binds insulin-like growth factor I and II (IGF-I and IGF-II). Partially purified preparations of this protein have been reported to inhibit the biologic actions of the IGFs. In these studies our laboratory has used a modified purification procedure to obtain a homogeneous preparation of this protein as determined by polyacrylamide gel electrophoresis and amino acid sequence analysis. During purification the ion exchange chromatography step resulted in two peaks of material with IGF binding activity termed peaks B and C. Each peak was purified separately to homogeneity. Both peaks were estimated to be 31,000 daltons by polyacrylamide gel electrophoresis and their amino acid compositions were nearly identical. Amino acid sequence analysis showed that both peaks had identical N-terminal sequences through the first 28 residues. Neither protein had detectable carbohydrate side chains and each had a similar affinity for radiolabeled IGF-I (1.7-2.2 x 10(10) liters/mol). In contrast, these two forms had marked differences in bioactivity. Concentrations of peak C material between 2 and 20 ng/ml inhibited IGF-I stimulation of [3H]thymidine incorporation into smooth muscle cell DNA. In contrast, when peak B (100 ng/ml) was incubated with IGF-I there was a 4.4-fold enhancement of stimulation of DNA synthesis. Additionally, pure peak B was shown to adhere to cell surfaces, whereas peak C was not adherent. The non-adherent peak C inhibited IGF-I binding to its receptor and to adherent peak B. We conclude that human amniotic fluid contains two forms of IGF binding protein that have very similar physiochemical characteristics but markedly different biologic actions. Since both have similar if not identical amino acid compositions, N-terminal sequences, and do not contain carbohydrate, we conclude that they differ in some other as yet undefined post-translational modification

Performance and Carcass Traits of Market Beef Cattle Supplemented Self-Fed Byproducts on Pasture: Final Report

Over a two year period (2007 and 2008), 162 head of beef steers were finished with self-fed byproducts on cool season grass pastures. Yearling steers were continuously grazed at the Neely-Kinyon Farm in southwest Iowa on cool season grasses that were predominantly fescue at a stocking density of 2.25 head/acre. Half of the cattle were implanted (with Synovex®-S) or half were not. Cattle received a diet of either soyhulls-dried distillers grain with solubles (DDGS) or ground corn-dried distillers grains with solubles that was offered through self-feeders. The rations were mixed at a 1:1 ratio with a mineral balancer added which included Rumensin®. Live cattle performance and carcass traits were not affected by diet. Implanted cattle outgained non-implanted over the entire finishing period (3.52 lbs/d vs. 3.17 lbs/d). This led to implanted cattle coming off test heavier (1324 lbs vs. 1277 lbs) and railing with heavier carcasses (826 lbs vs. 800 lbs). Ribeye areas were greater (13.1 in2 vs. 12.7 in2) for implanted cattle; which was probably due to the heavier carcass weights. Non-implanted cattle had superior quality grades (55% vs. 40%) of low choice or better. Fatty acid profiles from the first year were analyzed and showed that raw beef samples from cattle on the soyhulls diet had significantly higher C18:2 In conclusion, pasture rearing cattle, when given access to self-fed by-products, provides for excellent performance on both live performance and carcass traits. Some considerations should be made by the feeder in regards to time of year when marketing cattle and the cattle’s genetics. This system is an alternative to high-grain conventional beef finishing production in feedlots

Performance and Carcass Traits of Market Beef Cattle Supplemented Self-Fed Byproducts on Pasture: A Progress Report

Over a two year period (2007 and 2008), 162 head of beef steers were finished with self-fed byproducts on cool season grass pastures. Yearling steers were continuously grazed at the Neely-Kinyon Farm in southwest Iowa on cool season grasses that were predominantly fescue at a stocking density of 2.25 head/acre. Half of the cattle were implanted (with Synovex®-S) or and half were not. Cattle received a diet of either soyhulls-dried distillers grain with solubles or corn-dried distillers grains with solubles (DDGS) that was offered as a meal through self-feeders. The rations were mixed in at 1:1 with a mineral balancer that included Rumensin®. Live cattle performance and carcass traits were not affected by diet. Implanted cattle outgained non-implanted over the entire finishing period (3.52 lbs/d vs. 3.17 lbs/d). This led to implanted cattle coming off test heavier (1324 lbs vs. 1277 lbs) and railing with heavier carcasses (826 lbs vs. 800 lbs). Ribeye areas were greater (13.1 in 2 vs. 12.7 in 2 ) for implanted cattle; which was probably due to the heavier carcass weights. Non-implanted cattle had superior quality grades (55% vs. 40%) of low choice or better. Year differences in quality grade (1023 vs. 985 in 2007 and 2008, respectively) were observed. This difference was attributed to factors that include genetic makeup of cattle, initial weights of cattle, time of year when cattle were harvested and grading technology. In conclusion, pasture rearing cattle, when given access to self-fed by-products, provides for excellent performance on both live performance and carcass traits. Some considerations should be made by the feeder in regards to time of year when marketing cattle and the cattle’s genetics. This system is an alternative to high-grain conventional beef finishing production in feedlots

Rationale for a Permanent Seismic Network in the U.S. Central Plains Utilizing USArray

The eastern two thirds of the coterminous United States (from the Rocky Mountain Front to the east coast) are sparsely equipped with seismic monitoring instruments, with the number of permanent broadband seismic stations per unit area of the order of 5–10% of that in the western U.S. orogenic zone. In this Forum, we use the Central Plains area (CP)—defined here as the fourstate area including Nebraska, Kansas, Iowa, and Missouri—as an example to argue that a greatly densified permanent seismic network in the stable part of the United States could significantly improve our understanding of the processes that led to the formation and four-dimensional structure of the continental lithosphere. The network would also serve as an excellent facility for longterm earthquake monitoring and for public education and outreach. This issue is timely because a state-of-the-art, uniform network could be established by simply converting a small portion of the portable stations in the ongoing USArray project into permanent ones without affecting the overall progress of the USArray

Iron Age and Anglo-Saxon genomes from East England reveal British migration history

British population history has been shaped by a series of immigrations, including the early Anglo-Saxon migrations after 400 CE. It remains an open question how these events affected the genetic composition of the current British population. Here, we present whole-genome sequences from 10 individuals excavated close to Cambridge in the East of England, ranging from the late Iron Age to the middle Anglo-Saxon period. By analysing shared rare variants with hundreds of modern samples from Britain and Europe, we estimate that on average the contemporary East English population derives 38% of its ancestry from Anglo-Saxon migrations. We gain further insight with a new method, rarecoal, which infers population history and identifies fine-scale genetic ancestry from rare variants. Using rarecoal we find that the Anglo-Saxon samples are closely related to modern Dutch and Danish populations, while the Iron Age samples share ancestors with multiple Northern European populations including Britain