Large-scale analysis of peptide sequence variants : the case for high-field asymmetric waveform ion mobility spectrometry

[Image: see text] Large scale analysis of proteins by mass spectrometry is becoming increasingly routine; however, the presence of peptide isomers remains a significant challenge for both identification and quantitation in proteomics. Classes of isomers include sequence inversions, structural isomers, and localization variants. In many cases, liquid chromatography is inadequate for separation of peptide isomers. The resulting tandem mass spectra are composite, containing fragments from multiple precursor ions. The benefits of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for proteomics have been demonstrated by a number of groups, but previously work has focused on extending proteome coverage generally. Here, we present a systematic study of the benefits of FAIMS for a key challenge in proteomics, that of peptide isomers. We have applied FAIMS to the analysis of a phosphopeptide library comprising the sequences GPSGXVpSXAQLX(K/R) and SXPFKXpSPLXFG(K/R), where X = ADEFGLSTVY. The library has defined limits enabling us to make valid conclusions regarding FAIMS performance. The library contains numerous sequence inversions and structural isomers. In addition, there are large numbers of theoretical localization variants, allowing false localization rates to be determined. The FAIMS approach is compared with reversed-phase liquid chromatography and strong cation exchange chromatography. The FAIMS approach identified 35% of the peptide library, whereas LC–MS/MS alone identified 8% and LC–MS/MS with strong cation exchange chromatography prefractionation identified 17.3% of the library

Measurements of π±\pi ^\pm , K±K^\pm , p and pˉ\bar{p} spectra in 7^7Be+9^9Be collisions at beam momenta from 19A to 150A GeV ⁣/ ⁣c{\mathrm{Ge} \mathrm{V}}\!/\!c with the NA61/SHINE spectrometer at the CERN SPS

The NA61/SHINE experiment at the CERN Super Proton Synchrotron (SPS) studies the onset of deconfinement in hadron matter by a scan of particle production in collisions of nuclei with various sizes at a set of energies covering the SPS energy range. This paper presents results on inclusive double-differential spectra, transverse momentum and rapidity distributions and mean multiplicities of π ± π± , K ± K± , p and p ¯ p¯ produced in the 20% most central 7 7 Be+ 9 9 Be collisions at beam momenta of 19A, 30A, 40A, 75A and 150A GeV/c GeV/c . The energy dependence of the K ± K± /π ± π± ratios as well as of inverse slope parameters of the K ± K± transverse mass distributions are close to those found in inelastic p+p reactions. The new results are compared to the world data on p+p and Pb+Pb collisions as well as to predictions of the Epos, Urqmd, Ampt, Phsd and Smash models

Measurements of π±\pi ^\pm , K±K^\pm , p and pˉ\bar{p} spectra in 7^7Be+9^9Be collisions at beam momenta from 19A to 150A GeV ⁣/ ⁣c{\mathrm{Ge} \mathrm{V}}\!/\!c with the NA61/SHINE spectrometer at the CERN SPS

The NA61/SHINE experiment at the CERN Super Proton Synchrotron (SPS) studies the onset of deconfinement in hadron matter by a scan of particle production in collisions of nuclei with various sizes at a set of energies covering the SPS energy range. This paper presents results on inclusive double-differential spectra, transverse momentum and rapidity distributions and mean multiplicities of π ± π± , K ± K± , p and p ¯ p¯ produced in the 20% most central 7 7 Be+ 9 9 Be collisions at beam momenta of 19A, 30A, 40A, 75A and 150A GeV/c GeV/c . The energy dependence of the K ± K± /π ± π± ratios as well as of inverse slope parameters of the K ± K± transverse mass distributions are close to those found in inelastic p+p reactions. The new results are compared to the world data on p+p and Pb+Pb collisions as well as to predictions of the Epos, Urqmd, Ampt, Phsd and Smash models

Measurements of Ξ(1530)0{\Xi \left( 1530\right) ^{0}} and Ξ‾(1530)0{\overline{\Xi }\left( 1530\right) ^{0}} production in proton–proton interactions at sNN\sqrt{s_{NN}} = 17.3 = 17.3 GeV \text{ GeV } in the NA61/SHINE experiment

Double-differential yields of $\Xi\left(1530\right)^{0}$ and $\overline{\Xi}\left(1530\right)^{0}$ resonances produced in \pp interactions were measured at a laboratory beam momentum of 158~\GeVc. This measurement is the first of its kind in \pp interactions below LHC energies. It was performed at the CERN SPS by the \NASixtyOne collaboration. Double-differential distributions in rapidity and transverse momentum were obtained from a sample of 26$\cdot$10$^6$ inelastic events. The spectra are extrapolated to full phase space resulting in mean multiplicity of $\Xi\left(1530\right)^{0}$ (6.73 $\pm$ 0.25 $\pm$ 0.67)$\times10^{-4}$ and $\overline{\Xi}\left(1530\right)^{0}$ (2.71 $\pm$ 0.18 $\pm$ 0.18)$\times10^{-4}$. The rapidity and transverse momentum spectra and mean multiplicities were compared to predictions of string-hadronic and statistical model calculations