Lipid Bodies in Eremosphaera viridis De Bary (Chlorophyceae)

Under conditions of stress, e.g., nitrogen deficiency, Eremosphaera viridis De Bary (Chlorophyceae, Chlorococcales) synthesized secondary carotenoids and large amounts of triacylglycerols forming orange-red, cytosolic lipid bodies. Additionally, four polypeptides (28, 26, 25 and 23 kDa) as well as traces of chlorophyll a and b, of violaxanthin, neoxanthin and zeaxanthin, and of membrane lipids could be demonstrated in isolated lipid bodies. No membrane could be shown around the lipid bodies by the use of electron microscopy. The formation of lipid bodies in Eremosphaera is discussed as a bulging of the chloroplast envelope membrane

Bibliothek Dr. Georg Kloss : und Beiträge aus anderem Besitz

Katalog Buchhandlung und Antiquariat St. Goar. Teil I: Katalog 119: Bibliographie nebst Kunstwesen / Selten Drucke ; Anhang: Verlags- und Partieartikel Teil II: Katalog 120: Geschichte nebst Hilfswissenschafte

Vergleich einer Düngung mit Gülle und Schwefel bei Weizen

Da der atmosphärische Schwefeleintrag in den letzten Jahrzehnten deutlich verringert wurde, stellt sich auch im ökologischen Pflanzenbau die Frage, ob S ein limitierender Nährstoff ist. Um diese für das wichtige Backgetreide Winterweizen zu beantworten, wurden Feldversuche an drei Standorten über vier Jahre durchgeführt. Geprüft wurde eine Sulfatdüngung mit einer im ökologischen Landbau verbreiteten Gülledüngung jeweils einzeln oder kombiniert. Ausgebracht wurden 40 kg S bzw. NGesamt/ha im zeitigen Frühjahr mit Ausnahme der Güllegabe auf einem der drei Standorte, welche erst zu BBCH 32 erfolgte. Durch die Güllegabe wurde der Kornertrag des Weizens um neun Prozent erhöht und die Backqualität (Rohproteingehalt, Sedimentationswert, Feuchtkleber und Backvolumen) verbessert. Dagegen beeinflusste die Schwefeldüngung weder den Kornertrag noch Rohproteingehalt, Sedimentationswert und Feuchtkleber. Nur das Backvolumen war höher. Demnach kann Ertrag und Qualität von Weizen v. a. über eine Güllegabe beeinflusst werden

Towards Complete Sets of Farnesylated and Geranylgeranylated Proteins

Three different prenyltransferases attach isoprenyl anchors to C-terminal motifs in substrate proteins. These lipid anchors serve for membrane attachment or protein–protein interactions in many pathways. Although well-tolerated selective prenyltransferase inhibitors are clinically available, their mode of action remains unclear since the known substrate sets of the various prenyltransferases are incomplete. The Prenylation Prediction Suite (PrePS) has been applied for large-scale predictions of prenylated proteins. To prioritize targets for experimental verification, we rank the predictions by their functional importance estimated by evolutionary conservation of the prenylation motifs within protein families. The ranked lists of predictions are accessible as PRENbase (http://mendel.imp.univie.ac.at/sat/PrePS/PRENbase) and can be queried for verification status, type of modifying enzymes (anchor type), and taxonomic distribution. Our results highlight a large group of plant metal-binding chaperones as well as several newly predicted proteins involved in ubiquitin-mediated protein degradation, enriching the known functional repertoire of prenylated proteins. Furthermore, we identify two possibly prenylated proteins in Mimivirus. The section HumanPRENbase provides complete lists of predicted prenylated human proteins—for example, the list of farnesyltransferase targets that cannot become substrates of geranylgeranyltransferase 1 and, therefore, are especially affected by farnesyltransferase inhibitors (FTIs) used in cancer and anti-parasite therapy. We report direct experimental evidence verifying the prediction of the human proteins Prickle1, Prickle2, the BRO1 domain–containing FLJ32421 (termed BROFTI), and Rab28 (short isoform) as exclusive farnesyltransferase targets. We introduce PRENbase, a database of large-scale predictions of protein prenylation substrates ranked by evolutionary conservation of the motif. Experimental evidence is presented for the selective farnesylation of targets with an evolutionary conserved modification site

Enchondromas and atypical cartilaginous tumors at the proximal humerus treated with intralesional resection and bone cement filling with or without osteosynthesis: retrospective analysis of 42 cases with 6 years mean follow-up

Background: Enchondromas and atypical cartilaginous tumors (ACT) are often located at the proximal humerus. Most lesions can be followed conservatively, but surgical resection may alleviate pain, avoid pathological fractures, and prevent transformation into higher grade chondrosarcomas. Rigorous intralesional resection and filling with polymethylmethacrylate bone cement has been proposed for enchondromas but also for ACT, as an alternative for extralesional resection. We intended to analyze radiological, clinical, and functional outcome of this strategy and compare bone cement without osteosynthesis to bone cement compound osteosynthesis, which has not been analyzed so far. Methods: We retrospectively analyzed 42 consecutive patients (mean follow-up 73 months; range 8–224) after curettage and bone cement filling with or without osteosynthesis. Exclusion criteria were Ollier’s disease and cancellous bone filling. Twenty-five patients only received bone cement. Seventeen patients received additional proximal humerus plate for compound osteosynthesis to increase stability after curettage. Demographics and radiological and clinical outcome were analyzed including surgery time, blood loss, hospitalization, recurrences, and complications. An additional telephone interview at the final follow-up assessed postoperative satisfaction, pain, and function in the quick disabilities of the arm, shoulder, and hand (DASH) score and the Musculoskeletal Tumor Society (MSTS) score. Statistics included the Student T tests, Mann-Whitney U tests, and chi-square tests. Results: No osteosynthesis compared to compound osteosynthesis showed smaller tumors (4.2 (± 1.5) cm versus 6.6 (± 3.0) cm; p = 0.005) and smaller bone cement fillings after curettage (5.7 (± 2.1) cm versus 9.6 (± 3.2) cm; p = 0.0001). A score evaluating preoperative scalloping and soft-tissue extension did not significantly differ (1.9 (± 0.9) versus 2.0 (± 1.0); rating scale 0–4; p = 0.7). Both groups showed high satisfaction (9.2 (± 1.5) versus 9.2 (± 0.9); p = 0.5) and low pain (1.0(±1.7) versus 1.9(±1.8); p = 0.1) in a rating scale from 0 to 10. Clinical and functional outcome was excellent for both groups in the DASH score (6.0 (± 11.8) versus 11.0 (± 13.2); rating scale 0–100; p = 0.2) and the MSTS score (29.0 (± 1.7) versus 28.7 (± 1.1); rating scale 0–30; p = 0.3). One enchondroma recurrence was found in the group without osteosynthesis. Complications (one fracture and one intra-articular screw) were only detected after osteosynthesis. Osteosynthesis had longer surgery time (70 (± 21) min versus 127 (± 22) min; p < 0.0001), more blood loss (220 (± 130) ml versus 460 (± 210) ml; p < 0.0001), and longer stay in the hospital (6 (± 2) days versus 8 (± 2) days; p = 0.004). Conclusions: Intralesional tumor resection was oncologically safe and clinically successful with or without osteosynthesis. Osteosynthesis did not reduce the risk for fracture but was more invasive

Misbehaviour Prediction for Autonomous Driving Systems

Deep Neural Networks (DNNs) are the core component of modern autonomous driving systems. To date, it is still unrealistic that a DNN will generalize correctly in all driving conditions. Current testing techniques consist of offline solutions that identify adversarial or corner cases for improving the training phase, and little has been done for enabling online healing of DNN-based vehicles. In this paper, we address the problem of estimating the confidence of DNNs in response to unexpected execution contexts with the purpose of predicting potential safety-critical misbehaviours such as out of bound episodes or collisions. Our approach SelfOracle is based on a novel concept of self-assessment oracle, which monitors the DNN confidence at runtime, to predict unsupported driving scenarios in advance. SelfOracle uses autoencoder and time-series-based anomaly detection to reconstruct the driving scenarios seen by the car, and determine the confidence boundary of normal/unsupported conditions. In our empirical assessment, we evaluated the effectiveness of different variants of SelfOracle at predicting injected anomalous driving contexts, using DNN models and simulation environment from Udacity. Results show that, overall, SelfOracle can predict 77% misbehaviours, up to 6 seconds in advance, outperforming the online input validation approach of DeepRoad by a factor almost equal to 3.Comment: 11 page

CFTR mutations of patients with cystic fibrosis from Republic of Moldova

Departamentul de pediatrie, clinica de pneumologie, Universitatea de Stat de Medicină și Farmacie „Nicolae Testemiţanu”, Chișinău, Republica Moldova, Universitäts-Kinderklinik, Johann-Wolfgang-Goethe Universität, Frankfurt am Main, Germania, Service de génétique médicale, CHU de Bordeaux, Groupe Hospitalier Pellegrin, Bordeaux, Franţa, Klinische Forschergruppe‚ Molekulare Pathologie der Mukoviszidose, Klinik für Pädiatrische Pneumologie, Allergologie und Neonatologie, Medizinische Hochschule Hannover, Germania, ViennaLab Diagnostics GmbH, Wien, AustriaIntroducere. Spectrul de mutaţii ce cauzează fibroză chistică (FC) în Republica Moldova, deocamdată, nu a fost determinat. Material și metode. Analiza mutaţiei genei CFTR a fost efectuată pe mostre de ADN, colectate de la 61 de pacienţi cu FC, examinaţi în Departamentul de pediatrie, clinica de pneumologie, Universitatea de Stat de Medicină și Farmacie „Nicolae Testemiţanu”, în perioada anilor 2006-2011.Rezultate. Mutaţiile cu o frecvenţă mai mare de 3% au fost următoarele: F508del (57%), 2789+5 G-A (4%), 2184insA (4%) și G542X (3%). Concluzii. Distribuţia mutaţiei genei CFTR în Moldova este similară cu cea din România.Introduction. The spectrum of cystic fibrosis (CF) – cau-sing mutations in Republic of Moldova has yet not been determined. Material and methods. CFTR gene mutation analysis was performed on DNA samples from 61 patients with CF seen 2006-2011 at the Department of pediatrics, Division of pneumology, Nicolae Testemitanu State Medical and Pharmaceutical University. Results. Mutations with a frequency of more than 3% were F508del (57%), 2789+5 G-A (4%), 2184insA (4 %) and G542X (3%). Conclusions. The distribution of CFTR gene mutations in Moldova is similar to that in Romania.https://stiinta.usmf.md/sites/default/files/2018-09/MJHS%20nr.%203%20_2015.pd

Effects of cardiovascular single pill combinations compared with identical multi-pill therapies on healthcare cost and utilization in Germany

Aim: This study assessed whether a single pill combination (SPC) is associated with lower direct healthcare costs. Materials & methods: Anonymized claims data of patients ≥18 years treated with drugs for cardiovascular (CV)-related diseases either as a single pill combination or multi-pill combination (followup to 1 year) were evaluated. After propensity score matching, 59,336 out of 1,369,840 patients were analyzed. Results: In all cohorts, patients receiving a single pill combination had a lower frequency of general practitioner and specialist visits. The patients also had a significantly lower ratio of all-cause hospitalization days and number of CV-related prescriptions as well as all-cause prescriptions (with one exception) compared with those receiving a multi-pill combination. Conclusion: Direct CV-related costs were significantly lower in four out of seven comparisons, with a trend toward lower costs in the other three comparisons