Temporal dynamics in an immunological synapse:role of thermal fluctuations in signaling

The article analyzes the contribution of stochastic thermal fluctuations in the attachment times of the immature T-cell receptor TCR: peptide-major-histocompatibility-complex pMHC immunological synapse bond. The key question addressed here is the following: how does a synapse bond remain stabilized in the presence of high-frequency thermal noise that potentially equates to a strong detaching force? Focusing on the average time persistence of an immature synapse, we show that the high-frequency nodes accompanying large fluctuations are counterbalanced by low-frequency nodes that evolve over longer time periods, eventually leading to signaling of the immunological synapse bond primarily decided by nodes of the latter type. Our analysis shows that such a counterintuitive behavior could be easily explained from the fact that the survival probability distribution is governed by two distinct phases, corresponding to two separate time exponents, for the two different time regimes. The relatively shorter timescales correspond to the cohesion:adhesion induced immature bond formation whereas the larger time reciprocates the association:dissociation regime leading to TCR:pMHC signaling. From an estimate of the bond survival probability, we show that, at shorter timescales, this probability PΔ(τ) scales with time τ as a universal function of a rescaled noise amplitude DΔ2, such that PΔ(τ)∼τ-(ΔD+12),Δ being the distance from the mean intermembrane (T cell:Antigen Presenting Cell) separation distance. The crossover from this shorter to a longer time regime leads to a universality in the dynamics, at which point the survival probability shows a different power-law scaling compared to the one at shorter timescales. In biological terms, such a crossover indicates that the TCR:pMHC bond has a survival probability with a slower decay rate than the longer LFA-1:ICAM-1 bond justifying its stability

Contact time periods in immunological synapse

This paper resolves the long standing debate as to the proper time scale τ of the onset of the immunological synapse bond, the noncovalent chemical bond defining the immune pathways involving T cells and antigen presenting cells. Results from our model calculations show τ to be of the order of seconds instead of minutes. Close to the linearly stable regime, we show that in between the two critical spatial thresholds defined by the integrin:ligand pair (Δ2∼ 40-45 nm) and the T-cell receptor TCR:peptide-major-histocompatibility-complex pMHC bond (Δ1∼ 14-15 nm), τ grows monotonically with increasing coreceptor bond length separation δ (= Δ2-Δ1∼ 26-30 nm) while τ decays with Δ1 for fixed Δ2. The nonuniversal δ-dependent power-law structure of the probability density function further explains why only the TCR:pMHC bond is a likely candidate to form a stable synapse

Evaluation of Mobile Advanced Road Weather Information Sensor (MARWIS) by Lufft for Indiana Winter Road Operations

The students of the Field Infrastructure Assessment course evaluated the Mobile Advanced Road Weather Information Sensor by Lufft on behalf of the Indiana Department of Transportation. The device is mounted on a vehicle, takes roadway condition measurements 100 times per second, and aggregates the measurements into one-second intervals. The data is transmitted real-time to the cloud. The class specifically evaluated the following measurements: road condition, surface temperature, and friction. It was found that the MARWIS temperature reading was systematically 4˚F lower than the probe readings. Skid test empirical results were consistent with the MARWIS friction readings. It was also found, using two devices on the back of the test vehicle, that the friction in the wheel track was greater than the friction in the center of the lane, as expected. Lastly, the “chemically wet” road condition was not consistently observed by MARWIS where expected

Plans for Crash-Tested Bridge Railings for Longitudinal Wood Decks on Low-Volume Roads

The plans for crashworthy bridge railings for low-volume roads were developed through a cooperative research program involving the USDA Forest Service, Forest Products Laboratory (FPL); the Midwest Roadside Safety Facility, University of Nebraska-Lincoln (MwRSF); and the Forest Service, National Forest System, Engineering. Three railings were developed and successfully tested in accordance with National Cooperative Highway Research Program (NCHRP) Report 350 Test Level-1 requirements. The fourth system was developed for a lower test level based on criteria developed by the Forest Service for single-lane bridges on very low-volume roads. For the convenience of the user, full drawing sets are provided in customary U.S. and S.I. units

Estimation of the stress related to conservative scoliosis therapy: an analysis based on BSSQ questionnaires

BACKGROUND: Adolescent girls treated with a brace for scoliosis are submitted to prolonged stress related to both the disease and the therapy. Currently proposed quality of life questionnaires are focused on the outcome of therapy. Bad Sobernheim Stress Questionnaire (BSSQ) enables monitoring of patients being under treatment with a brace or exercises. The aim of the study was to assess the stress level in conservatively managed scoliotic girls using BSSQ. MATERIALS AND METHODS: 111 girls, aged 14,2 ± 2,2 years, mean Cobb angle of the primary curve 42,8° ± 17,0° and mean Bunnell angle of 11,4° ± 4,5° were examined with two versions of BSSQ (Deformity and Brace). The analysis considered the type of treatment, curve location, correlation of the total score with age, Cobb angle and Bunnell rotation angle. RESULTS: The BSSQ Deformity revealed the median of 17 points in patients managed with exercises (from 4 to 24 points), 18 in patients managed with a brace (from 8 to 24 points) and 12 in patients before surgery (from 3 to 21 points). Braced patients who completed both questionnaires (n = 50) revealed significantly higher score with BSSQ Deformity (median = 18) comparing to BSSQ Brace (median = 9). There was a correlation between the total score of BSSQ Deformity and the Cobb angle (r = -0,34), Bunnell primary curve rotation (r = -0,34) and Bunnell sum of rotation (r = -0,33) but not with the age of patients. CONCLUSION: Scoliotic adolescents managed with exercises and brace suffered little stress from the deformity. The brace increased the level of stress over the stress induced by the deformity. The stress level correlated with clinical deformity (Bunnell angle), radiological deformity (Cobb angle) and the type of treatment (exercises, bracing, surgery). Bad Sobernheim Stress Questionnaires are simple and helpful in the management of girls treated conservatively for idiopathic scoliosis

Linking Adult Reproduction and Larval Density of Invasive Carp in a Large River

Eeotogists increasingly recognize the need to understand how landscapes ami food webs interact. Reservoir ecosystems are heavily subsidized by nutrients and detritus from surrounding watersheds, and ofren contain abundant populations of gizzard shad, an omnivorous ftsh that consumes plankton and detritus. Gizzard shad link terrestrial landscapes ami pelagic reservoir food webs by consuming detritus, translocating nutrients from sedimctn detritus to the water column, and consuming zooplaukton. The abundance of gizzard shad increases with watershed agricuhuralization, most likely through n variety oj mechanisms npeniting on ttuvat and adult life stages. Gizzard shad have myriad effects on reservoirs, including impacts on nutrients, phytoplankton, zooplankton, and fish, and many of their effects vary with ecosystem productivity (i.e., watershed land use). Interactive feedbacks among watersheds, gizzard shad populations, and reservoir food webs operate to maintain dominance of gizzard shad in highly productive systems. Thus, effective stewardship of reservoir ecosystems must incorporate both watershed and food-web perspective

Evaluating consumptive and nonconsumptive predator effects on prey density using field time‐series data

Determining the degree to which predation affects prey abundance in natural communities constitutes a key goal of ecological research. Predators can affect prey through both consumptive effects (CEs) and nonconsumptive effects (NCEs), although the contributions of each mechanism to the density of prey populations remain largely hypothetical in most systems. Common statistical methods applied to time‐series data cannot elucidate the mechanisms responsible for hypothesized predator effects on prey density (e.g., differentiate CEs from NCEs), nor can they provide parameters for predictive models. State‐space models (SSMs) applied to time‐series data offer a way to meet these goals. Here, we employ SSMs to assess effects of an invasive predatory zooplankter, Bythotrephes longimanus, on an important prey species, Daphnia mendotae, in Lake Michigan. We fit mechanistic models in an SSM framework to seasonal time series (1994–2012) using a recently developed, maximum‐likelihood–based optimization method, iterated filtering, which can overcome challenges in ecological data (e.g., nonlinearities, measurement error, and irregular sampling intervals). Our results indicate that B. longimanus strongly influences D. mendotae dynamics, with mean annual peak densities of B. longimanus observed in Lake Michigan estimated to cause a 61% reduction in D. mendotae population growth rate and a 59% reduction in peak biomass density. Further, the observed B. longimanus effect is most consistent with an NCE via reduced birth rates. The SSM approach also provided estimates for key biological parameters (e.g., demographic rates) and the contribution of dynamic stochasticity and measurement error. Our study therefore provides evidence derived directly from survey data that the invasive zooplankter B. longimanus is affecting zooplankton demographics and offer parameter estimates needed to inform predictive models that explore the effect of B. longimanus under different scenarios, such as climate change.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/148243/1/ecy2583-sup-0001-AppendixS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148243/2/ecy2583_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148243/3/ecy2583.pd