Evaluation of multidrug resistance-1 gene C>;T polymorphism frequency in patients with asthma

OBJECTIVES:Asthma is a chronic inflammatory lung disease characterized by bronchial hyperresponsiveness and airflow obstruction. Genetic and oxidative stress factors, in addition to pulmonary and systemic inflammatory processes, play a pivotal role in the pathogenesis of asthma. The products of the multidrug resistance-1 gene protect lung tissue from oxidative stress. Here, we aimed to evaluate the association between the multidrug resistance-1 gene C>;T polymorphism and asthma with regard to oxidative stress-related parameters of asthmatic patients.METHODS:Forty-five patients with asthma and 27 healthy age-matched controls were included in this study. Blood samples were collected in tubes with ethylenediaminetetraacetic acid. DNA was extracted from the blood samples. The multidrug resistance-1 gene polymorphism was detected by polymerase chain reaction and a subsequent enzyme digestion technique. The serum levels of total oxidant status and total antioxidant status were determined by the colorimetric measurement method.RESULTS:The heterozygous polymorphic genotype was the most frequent in both groups. A significant difference in the multidrug resistance-1 genotype frequencies between groups indicated an association of asthma with the TT genotype. A significant difference between groups was found for wild type homozygous participants and carriers of polymorphic allele participants. The frequency of the T allele was significantly higher in asthmatic patients. The increase in the oxidative stress index parameter was significant in the asthma group compared with the control group.CONCLUSIONS:The multidrug resistance-1 gene C/T polymorphism may be an underlying genetic risk factor for the development of asthma via oxidant-antioxidant imbalance, leading to increased oxidative stress

Shifts in appraisal dimensions as mediators of efficiency of reappraisal in emotion regulation

Üks viis oma emotsiooni reguleerida on kasutada ümberhindamist ehk muuta oma tõlgendust emotsiooni tekitanud olukorrast. Kuigi tõlgendusdimensioonide rolli ümberhindamises on ammu teadvustatud, pole seda ideed ümberhindamise uurimisel seni kasutatud. Käesoleva uurimistöö eesmärk on uurida, kas ja kuivõrd võivad muutused tõlgendusdimensioonidel vahendada ümberhindamise mõju emotsiooni regulatsioonile. Igapäevaelus ettetulevate emotsioonide reguleerimise mõõtmiseks kasutati 7 päeva jooksul kogemuse väljavõtte meetodit. Tekstisõnumiga teavitades paluti uuritavatel kella 11, 13 ja 15 ajal täita küsimustiku päevane osa ning kell 19 küsimustiku õhtune kokkuvõtlik osa. Regressioonanalüüsidega uuriti tõlgendusdimensioonide muutuse ja emotsiooni muutuse vahelisi seoseid ning medieerimisanalüüsiga uuriti, kas ja kuivõrd tõlgendusdimensioonide muutused vahendavad ümberhindamise kasutamise mõju emotsioonide muutusele. Uuringus osales 181 inimest vanuses 18-52 aastat ( M =28.25; SD =7.75), kellest 85% olid naised. Saadud tulemused kinnitasid, et kolme analüüsitud emotsiooni - viha, ärevuse, lõõgastuse - muutused on arvestatavas osas seletatavad muutustega kindluse, olulisuse, eesmärgipärasuse, vastutuse ja kontrolli tõlgendusdimensioonidel. Tulemused näitavad ka, et eesmärgipärasus on universaalne tõlgendusdimensioon, mille hinnang tõuseb nii negatiivse emotsiooni vähendamisel kui ka positiivse emotsiooni suurendamisel. Medieerimisanalüüsid näitasid ainult lõõgastuse puhul, et tõlgendusdimensioonid vahendavad ümberhindamise kasutamise ja emotsiooni muutuse vahelist seost ning seda läbi muutuste eesmärgipärasuse, vastutuse ja kontrolli tõlgendusdimensioonidel. Käesolev uuring demonstreerib tõlgendusdimensioonide väärtust ümberhindamise käigus toimuvate kognitiivsete protsesside mõistmisel. Kuna tulemused on eripalgelised, vajab küsimus täiendavat uurimist

A comparative ID migraine screener study in ophthalmology, ENT and neurology out-patient clinics

Migraine is more likely to be misdiagnosed in patients with comorbid diseases. Not only primary care physicians, but also specialists might misdiagnose it due to the lack of diagnostic criteria awareness. The ID migraine test is a reliable screening instrument that may facilitate and accelerate migraine recognition. This study aimed to compare the prevalence and characteristics of migraine in a large sample of patients admitted to clinics of ophthalmology (OC), ear, nose and throat diseases (ENTC) and neurology (NC), as well as to validate the use of the ID migraine test in OC and ENTC settings. This was a multicentre (11 cites) study of out-patients admitting either to NC, ENTC or OC of the study sites during five consecutive working days within 1 week. From each of the clinics, 100 patients were planned to be recruited. All recruited patients were interviewed and those having a headache complaint received an ID migraine test and were examined for headache diagnosis by a neurologist, blinded to the ID migraine test result. A total of 2625 subjects were recruited. Only 1.3% of OC patients and 5.4% of ENTC patients have been admitted with a primary complaint of headache, whereas the percentage of NC patients suffering from headache was 37.6%. Whereas 138 patients (19.3%) in OC, 154 (17.3%) in ENTC and 347 (34%) in NC were found to be ID migraine test positive, 149 patients (20.8%) in OC, 142 (16%) in ENTC and 338 (33.1%) in NC were diagnosed with migraine. The sensitivity, specificity, and positive and negative predictive ratios of the ID migraine test were found to be similar in all clinics. An important fraction of the patients admitted to NC, as well as to OC and ENTC, for headache and/or other complaints were found out to have migraine by means of a simple screening test. This study validated the ID migraine test as a sensitive and specific tool in OC and ENTC, encouraging its use as a screening instrument.Pfizer-Türkiy

SAĞLIK PERSONELİNİN ELDİVEN KULLANIMINA İLİŞKİN HASTA GÖRÜŞLERİNİN İNCELENMESİ

DergiPark: 73078ejovocObjective: There is no study related to patients’ opinions about using gloves of health personnel. In this study aimed to investigate the views of patient opinions related to health personnel use of gloves. Methods: The population of this descriptive and cross sectional study was consisted of 340 patients who hospitalized in internal medicine and general surgery clinics, of two different status hospitals in the province of Aydın at least 3 days and sample was consisted of 168 patients who accepted to attended to study. The data were collected using a data collection form which consisted of multiple-choice and open ended 28 questions prepared by the researchers. Distributions of results were been given as percentage and numerical. Results: Approximately all (97.0%) of the patients were stated that health care workers should use gloves during invasive procedures, 74.4% of them were stated that the most disturbed administration is to implemented very much application with the same glove. Overall, the 98.8% of the patients pointed out that during giving an injection, 97.6 % during inserting a intravenous catheter and drawing blood should be use gloves, the 89.9% of the patients' were states that they felt using gloves is protected them against germs, 28.6% reported that they felt they were worthless in case of not using gloves. Conclusion: Based on this study, most of patients have state that health personnel should use gloves , they felt themselves valueless when health personnel don’t used gloves, they feel disturbed when they given care to different patients using the same glove, and nurses should used gloves more than physiciansAmaç: Hastaların sağlık çalışanlarının eldiven kullanımına ilişkin görüşleri ile ilgili çalışma bulunmamaktadır. Bu araştırmada hastaların sağlık personelinin eldiven kullanımına ilişkin görüşlerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Tanımlayıcı ve kesitsel türdeki bu araştırmanın evrenini Aydın ilindeki iki farklı statüdeki hastanenin dahiliye ve cerrahi kliniklerinde yatan tüm hastalar (340), örneklemini ise araştırma sınırlılıklarına uyan 168 hasta oluşturmuştur. Veriler araştırmacılar tarafından hazırlanan çoktan seçmeli ve açık uçlu 28 sorudan oluşan veri toplama formu ile toplanmıştır. Sonuçların sayısal ve yüzdelik dağılımları verilmiştir. Bulgular: Hastaların tamamına yakını (97.0%) invaziv işlemler sırasında hemşirelerin eldiven kullanması gerektiğini, % 74.4’ü en çok rahatsız oldukları uygulamanın aynı eldivenle pek çok iş yapılması olduğunu belirtmişlerdir. Hastaların %98.8’i enjeksiyon uygulamak, %97.6’sı kan alma ve intravenöz kateter uygulama işlemi sırasında eldiven kullanımının gerekli olduğunu, %89.9’u eldiven kullanılması durumunda mikroplara karşı korunduğunu hissettiğini, % 28.6’sı eldiven kullanılmaması durumunda değersiz olduklarını hissettiklerini belirtmişlerdir. Sonuç: Araştırmanın sonucunda hastaların çoğunluğu sağlık çalışanlarının eldiven kullanması gerektiğini, kullanmadıkları zaman kendilerini değersiz hissettiklerini, aynı eldivenle farklı hastalara bakım verilmesinden rahatsız olduklarını ve hemşirelerin doktorlara oranla daha çok eldiven kullanması gerektiğini belirtmişlerdi