A dynamic hydro-mechanical and biochemical model of stomatal conductance for C4 photosynthesis

C4 plants are major grain (maize, sorghum), sugar (sugarcane) and biofuel (Miscanthus) producers, and contribute ~20% to global productivity. Plants lose water through stomatal pores in order to acquire CO2 (assimilation, A), and control their carbon-for-water balance by regulating stomatal conductance (gS). The ability to mechanistically predict gS and A in response to atmospheric CO2, water availability and time is critical for simulating stomatal control of plant-atmospheric carbon and water exchange under current, past or future environmental conditions. Yet, dynamic mechanistic models for gS are lacking, especially for C4 photosynthesis. We developed and coupled a hydro-mechanical model of stomatal behaviour with a biochemical model of C4 photosynthesis, calibrated using gas exchange measurements in maize, and extended the coupled model with time- explicit functions to predict dynamic responses. We demonstrated the wider applicability of the model with three additional C4 grass species in which interspecific differences in stomatal behaviour could be accounted for by fitting a single parameter. The model accurately predicted steady-state responses of gS to light, atmospheric CO2 and O2, soil drying and evaporative demand, as well as dynamic responses to light intensity. Further analyses suggest the effect of variable leaf hydraulic conductance is negligible. Based on the model, we derived a set of equations suitable for incorporation in land surface models. Our model illuminates the processes underpinning stomatal control in C4 plants and suggests the hydraulic benefits associated with fast stomatal responses of C4 grasses may have supported the evolution of C4 photosynthesis

Variation in the quality of meat from Irish steers at the time of slaughter.

End of Project ReportThere is no information on the variation in quality, in particular tenderness, that exists in Irish Beef nor is there information on the variation that would remain if optimum practices were imposed at all stages of the beef production chain. Evaluation of the success of measures to improve beef consistency requires information on existing variation and the minimum variation achievable.The objectives of this project were (i) to establish the variation that exists in the quality of meat from Irish cattle, (ii) to quantify the minimum variation in meat quality that can be achieved in a practical beef production system, (iii) to determine the effects and mechanisms of additional sources of variation. The conclusions from this project are: • The M. longissimus dorsi (loin) was found to be more variable than the M. semimembranosus (topside) for most quality attributes examined (tenderness, sarcomere length and pH). The scale of variation within the loin was similar to that reported by the other research groups within the EU and US. Heifers were more variable than steers for most attributes, while there was no consistent classification effect on the variability of meat quality attributes. • Tenderness was equally variable in meat from genetically similar steers, managed similarly, compared to commercial steers randomly selected from a factory lairage but matched for weight and grade.This was likely a result of both groups being crossbred beef cattle of similar age, fat score, carcass weight and managed identically post-mortem. However, variation in tenderness of both groups was less than that observed in a survey of commercial throughput (experiment 1). This decrease is attributed to better pre-and-post-slaughter handling practices. • The data suggest that selection of sires (within a breed) with better than average conformation has no deleterious effect on the eating quality of beef of their progeny.A more comprehensive comparison of sires within a breed and between breeds is required to confirm the generality of this conclusion. • In a comparison of genotypes, gender and slaughter weights, there was no evidence that variation around the mean value for tenderness differed between breeds or liveweights after 14 days ageing. Bulls were more variable than steers for some quality traits but the variation in tenderness was similar for bulls and steers after 14 days ageing. • While optimising the management of animals during the pre and post-slaughter period reduced variation in tenderness, some residual variation remained. A large percentage of the residual variation in tenderness (Warner Bratzler shear force) after 2 and 7 days post-mortem was explained by proteolysis (breakdown of myofibrillar proteins).Variation in tenderness (Warner Bratzler shear force) after 2 days post-mortem was largely explained by phosphates (energy) and proteolysis, while sensory tenderness was largely explained by phosphates and glycolytic potential. • Further work is required to reduce residual variation in Irish beef and to determine the causes of this variation

Studies on Pre-slaughter Handling of Pigs and its Relationship to Meat Quality

End of Project ReportsTwo quality defects of pork which are affected by preslaughter handling are PSE (Pale Soft Exudative) and DFD (Dark Firm Dry) meat. The incidence of PSE pork is mainly a function of the breed of pig but short-term stressful handling before slaughter and feeding too close to slaughter are also involved. DFD meat is a result of prolonged stressful handling. PSE meat is pale and uneven in colour and exudes fluid making it unattractive in the retail display while dark meat appears stale and is prone to bacterial spoilage. After slaughter muscle metabolism continues and muscle glycogen is converted to lactic acid reducing meat pH. Prolonged stress results in glycogen depletion, pre-slaughter feeding results in elevated levels. Colour may be assessed subjectively by eye or objectively by a meter colour but pH of the meat is closely related to colour and measurement of pH at 45 minutes post-slaughter is frequently used to predict ultimate colour and pH. The objective of this study was to examine pre-slaughter handling practices and their relationship with meat quality (pH, colour). In the first trial, a survey of the amounts of stomach contents in pigs at slaughter in two factories found similar amounts to comparable surveys in France and the UK. It was concluded that most pigs had been fasted for an adequate time before delivery. The relationship between the amount of stomach contents and meat quality in this survey was poor. In the second trial, pigs from the Moorepark herd fed by either a computerised wet feeding system or an ad libitum dry feed system were slaughtered after overnight fasting or with feed available up to loading for transport to the factory, two to three hours before slaughter. There was no difference between feeding systems in meat colour or pH but fasted pigs, on both feeding systems, had darker meat and meat of a higher pH. In the third and fourth trials a survey of transport vehicles was carried out and meat quality of pigs delivered in modern and old-type vehicles was compared. Most trucks examined (78%) were four years old or more. Few had modern hydraulic lifting gear for the top decks. Space allowances during transportation were generally adequate but delays in unloading could, in warm weather, cause stress on pigs. There was little evidence for an effect of vehicle on meat quality parameters but day to day variation in carcass temperature and pH suggested a need for further research on factory influences on meat quality. Feeding of Magneium Aspartate to pigs for the last 5 days prior to slaughter has been shown, in Australia, to have a beneficial effect on meat colour and drip loss. In the final trial in this study Mg Asp had no effect on meat quality parameters.European Union Structural Funds (EAGGF

A geological model of the chalk of East Kent

This report describes the geological modelling of the Chalk in the North Downs of East Kent, within the catchment of River Great Stour and eastwards to the coast, including the Isle of Thanet. This work was funded by the Environment Agency to support investigations of the local hydrogeology and thereby to enhance catchment management. The whole area is underlain by the Upper Cretaceous Chalk Group, with the Palaeogene succession of the Thanet Sand Formation, the Lambeth Group and the Thames Group overlying it in the northern and central eastern parts. The project included a desk study revision of the Chalk of the North Downs, using the new Chalk lithostratigraphy. The revisions to the geology are shown on the 1:50 000 scale geological map which accompanies this report. Together with evidence from boreholes and from seismic surveys, the new outcrop patterns have been incorporated into a geological model, using both computer software (EarthVision) and manual methods. The introduction describes the background to the project. The second chapter describes the sources for the data used in the model: published and unpublished geological maps, borehole records (both lithological and geophysical), seismic surveys, biostratigraphic records, digital topographic information, and the published literature. Each Chalk formation present in the area is then briefly described in the third chapter, noting its relationship to the older lithostratigraphic divisions, and to biostratigraphic zones. The local Chalk succession extends from the base of the Chalk Group to the Newhaven Chalk Formation, here represented by the Margate Chalk Member. Evidence for the thickness of each formation is reviewed. The early Palaeogene formations (the Thanet Sand, Upnor, Harwich and London Clay formations) are also briefly described (Chapter 4) and the local superficial deposits mentioned, with references to detailed descriptions (Chapter 5). Apart from minor adjustments to the outcrop of the basal Palaeogene surface, no revision of these formations was done for this study

Correlated variability of Mkn 421 at X-ray and TeV wavelengths on timescales of hours

Mkn 421 was observed for about two days with BeppoSAX, prior to and partly overlapping the start of a 1 week continuous exposure with ASCA in April 1998, as part of a world-wide multiwavelength campaign. A pronounced, well defined, flare observed in X-rays was also observed simultaneously at TeV energies by the Whipple Observatory's 10 m gamma-ray telescope. These data provide the first evidence that the X-ray and TeV intensities are well correlated on time-scales of hours.Comment: 4 pages, 1 figure, presented at the VERITAS Workshop on the TeV Astrophysics of Extragalactic Object

Quantum field dynamics of the slow rollover in the linear delta expansion

We show how the linear delta expansion, as applied to the slow-roll transition in quantum mechanics, can be recast in the closed time-path formalism. This results in simpler, explicit expressions than were obtained in the Schr\"odinger formulation and allows for a straightforward generalization to higher dimensions. Motivated by the success of the method in the quantum-mechanical problem, where it has been shown to give more accurate results for longer than existing alternatives, we apply the linear delta expansion to four-dimensional field theory. At small times all methods agree. At later times, the first-order linear delta expansion is consistently higher that Hartree-Fock, but does not show any sign of a turnover. A turnover emerges in second-order of the method, but the value of $$at the turnover is larger that that given by the Hartree-Fock approximation. Based on this calculation, and our experience in the corresponding quantum-mechanical problem, we believe that the Hartree-Fock approximation does indeed underestimate the value of$$ at the turnover. In subsequent applications of the method we hope to implement the calculation in the context of an expanding universe, following the line of earlier calculations by Boyanovsky {\sl et al.}, who used the Hartree-Fock and large-N methods. It seems clear, however, that the method will become unreliable as the system enters the reheating stage.Comment: 17 pages, 9 figures, revised version with extra section 4.2 including second order calculatio

Are markers of inflammation more strongly associated with risk for fatal than for nonfatal vascular events?

<p><b>Background:</b> Circulating inflammatory markers may more strongly relate to risk of fatal versus nonfatal cardiovascular disease (CVD) events, but robust prospective evidence is lacking. We tested whether interleukin (IL)-6, C-reactive protein (CRP), and fibrinogen more strongly associate with fatal compared to nonfatal myocardial infarction (MI) and stroke.</p> <p><b>Methods and Findings:</b> In the Prospective Study of Pravastatin in the Elderly at Risk (PROSPER), baseline inflammatory markers in up to 5,680 men and women aged 70-82 y were related to risk for endpoints; nonfatal CVD (i.e., nonfatal MI and nonfatal stroke [n = 672]), fatal CVD (n = 190), death from other CV causes (n = 38), and non-CVD mortality (n = 300), over 3.2-y follow-up. Elevations in baseline IL-6 levels were significantly (p = 0.0009; competing risks model analysis) more strongly associated with fatal CVD (hazard ratio [HR] for 1 log unit increase in IL-6 1.75, 95% confidence interval [CI] 1.44-2.12) than with risk of nonfatal CVD (1.17, 95% CI 1.04-1.31), in analyses adjusted for treatment allocation. The findings were consistent in a fully adjusted model. These broad trends were similar for CRP and, to a lesser extent, for fibrinogen. The results were also similar in placebo and statin recipients (i.e., no interaction). The C-statistic for fatal CVD using traditional risk factors was significantly (+0.017; p<0.0001) improved by inclusion of IL-6 but not so for nonfatal CVD events (p = 0.20).</p> <p><b>Conclusions:</b> In PROSPER, inflammatory markers, in particular IL-6 and CRP, are more strongly associated with risk of fatal vascular events than nonfatal vascular events. These novel observations may have important implications for better understanding aetiology of CVD mortality, and have potential clinical relevance.</p&gt

Association of visit-to-visit variability in blood pressure with cognitive function in old age: prospective cohort study

<p>Objective To investigate the association between visit-to-visit variability in blood pressure and cognitive function in old age (>70 years).</p> <p>Design Prospective cohort study.</p> <p>etting PROSPER (PROspective Study of Pravastatin in the Elderly at Risk) study, a collaboration between centres in Ireland, Scotland, and the Netherlands.</p> <p>Participants 5461 participants, mean age 75.3 years, who were at risk of cardiovascular disease. Blood pressure was measured every three months during an average of 3.2 years. Visit-to-visit variability in blood pressure was defined as the standard deviation of blood pressure measurements between visits.</p> <p>Main outcome measures Four domains of cognitive function, testing selective attention, processing speed, and immediate and delayed memory. In a magnetic resonance imaging substudy of 553 participants, structural brain volumes, cerebral microbleeds, infarcts, and white matter hyperintensities were measured.</p> <p>Results Participants with higher visit-to-visit variability in systolic blood pressure had worse performance on all cognitive tests: attention (mean difference high versus low thirds) 3.08 seconds (95% confidence interval 0.85 to 5.31), processing speed −1.16 digits coded (95% confidence interval −1.69 to −0.63), immediate memory −0.27 pictures remembered (95% confidence interval −0.41 to −0.13), and delayed memory −0.30 pictures remembered (95% confidence interval −0.49 to −0.11). Furthermore, higher variability in both systolic and diastolic blood pressure was associated with lower hippocampal volume and cortical infarcts, and higher variability in diastolic blood pressure was associated with cerebral microbleeds (all P<0.05). All associations were adjusted for average blood pressure and cardiovascular risk factors.</p> Conclusion Higher visit-to-visit variability in blood pressure independent of average blood pressure was associated with impaired cognitive function in old age

Higher Order Evaluation of the Critical Temperature for Interacting Homogeneous Dilute Bose Gases

We use the nonperturbative linear \delta expansion method to evaluate analytically the coefficients c_1 and c_2^{\prime \prime} which appear in the expansion for the transition temperature for a dilute, homogeneous, three dimensional Bose gas given by T_c= T_0 \{1 + c_1 a n^{1/3} + [ c_2^{\prime} \ln(a n^{1/3}) +c_2^{\prime \prime} ] a^2 n^{2/3} + {\cal O} (a^3 n)\}, where T_0 is the result for an ideal gas, a is the s-wave scattering length and n is the number density. In a previous work the same method has been used to evaluate c_1 to order-\delta^2 with the result c_1= 3.06. Here, we push the calculation to the next two orders obtaining c_1=2.45 at order-\delta^3 and c_1=1.48 at order-\delta^4. Analysing the topology of the graphs involved we discuss how our results relate to other nonperturbative analytical methods such as the self-consistent resummation and the 1/N approximations. At the same orders we obtain c_2^{\prime\prime}=101.4, c_2^{\prime \prime}=98.2 and c_2^{\prime \prime}=82.9. Our analytical results seem to support the recent Monte Carlo estimates c_1=1.32 \pm 0.02 and c_2^{\prime \prime}= 75.7 \pm 0.4.Comment: 29 pages, 3 eps figures. Minor changes, one reference added. Version in press Physical Review A (2002