Micronucleus test and comet assay in erythrocytes of the Amazonian electric fish Apteronotus bonapartii exposed to benzene

In this study we address the genotoxicity and putative mutagenic effects of benzene (BZN) in the erythrocytes of the electric fish Apteronotus bonapartii (Gymnotiformes, Apteronotidae) using the micronucleus test (MN) and comet assay, under controlled laboratory conditions. Electric fish were collected in the Solimões River, Manaus-AM, Brazil, and the specimens were exposed to 10 and 25 ppm concentrations of BZN, in 150L tanks. Blood samples were collected at 0 (T0), 24, 48, 72 and 96 hours of exposure. For the concentration of 10 ppm BZN, the number of comets was significantly higher than T0 levels after exposure of 48 hours, whereas the nuclear abnormalities (including MN) did not show any increase in relation to the controls (T0) up to 96 hours. For the 25 ppm BZN, MN rates presented a significant increase after 72 hours, whereas other types of nuclear abnormalities increased in frequency after various exposure times, ranging from 24 to 72 hours. The number of comets increased significantly from 24 hours onwards for 25 ppm BZN. Both assays also showed a gradual increase in the number of damaged cells after longer exposure periods, indicating a time-dependent effect, especially at the highest BZN concentrations tested. This investigation reinforces the potential use of the endemic South American electric fish as a suitable genotoxicity biological model for biomonitoring purposes in the Amazon. Keywords: Comet assay, Micronucleus test, Apteronotus bonapartii, Benzene, Biomonitoring, Electric fish, Amazo

Broadband matched-field processing: coherent and incoherent approaches

Matched-field based methods always involve the comparison of the output of a physical model and the actual data. The method of comparison and the nature of the data varies according to the problem at hand, but the result becomes always largely conditioned by the accurateness of the physical model and the amount of data available. The usage of broadband methods has become a widely used approach to increase the amount of data and to stabilize the estimation process. Due to the difficulties to accurately predict the phase of the acoustic field the problem whether the information should be coherently or incoherently combined across frequency has been an open debate in the last years. This paper provides a data consistent model for the observed signal, formed by a deterministic channel structure multiplied by a perturbation random factor plus noise. The cross-frequency channel structure and the decorrelation of the perturbation random factor are shown to be the main causes of processor performance degradation. Different Bartlett processors, such as the incoherent processor [Baggeroer et al., J. Acoust. Soc. Am. 80, 571-587 (1988)], the coherent normalized processor [Z.-H. Michalopoulou, IEEE J. Ocean Eng. 21, 384-392 (1996)] and the matched-phase processor [Orris et al., J. Acoust. Soc. Am. 107, 2563-2375 (2000)], are reviewed and compared to the proposed cross-frequency incoherent processor. It is analytically shown that the proposed processor has the same performance as the matched-phase processor at the maximum of the ambiguity surface, without the need for estimating the phase terms and thus having an extremely low computational cost. (C) 2003 Acoustical Society of America

A Robust Adaptive Solution Strategy for High-Order Implicit CFD Solvers

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90694/1/AIAA-2011-3696-676.pd

Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith

Introduction: In this study, we used dichloromethane (DCM) and methanol (MeOH) extracts of the Zingiber zerumbet rhizome to evaluate brine shrimp lethality and larvicidal activity on Aedes aegypti and Anopheles nuneztovari mosquitoes. Methods: Bioassays were performed by exposing third-instar larvae of each mosquito species to the DCM or MeOH extracts. Results: Probit analysis with DCM and MeOH extracts demonstrated efficient larvicidal activity against A. aegypti and A. nuneztovari larvae. Conclusions: The DCM and MeOH extracts showed higher activity against A. nuneztovari larvae than against A. aegypti larvae, suggesting that the extracts have species-specific activity