Relative abundance of pelagic sharks in the western North Atlantic Ocean, including the Gulf of Mexico and Caribbean Sea

Little information exists on the status of pelagic shark populations in the Atlantic Ocean, especially in the Gulf of Mexico and Caribbean Sea. We derived indices of relative abundance for pelagic sharks based on mandatory logbooks and observer reports from a scientific observer program of the United States (US) pelagic longline fleet. Time series data from the pelagic longline logbook program (1986-2005) and the pelagic longline observer program (1992-2005) were standardized with Generalized Linear Model (GLM) procedures. Declines in relative abundance for the 6 pelagic shark species or genera examined in the logbook data analysis ranged from 43% for mako sharks, Isurus spp., to 88% for blue sharks, Prionace glauca, whereas declines in relative abundance obtained from the observer data analysis were less accentuated than those in the logbook data analysis, with the trend being positive for night sharks, Carcharhinus signatus, and thresher sharks, Alopias spp. There was no significant change in the fork length at capture over the time period considered for blue sharks, shortfin makos Isurus oxyrinchus, or night sharks. The trends obtained must be viewed cautiously given recognized shortcomings, especially of the logbook dataset, and the highly migratory nature of pelagic sharks, which requires a more comprehensive evaluation of trends throughout their range

On chromatic roots of large subdivisions of graphs

AbstractGiven a graph G, we derive an expression for the chromatic polynomials of the graphs resulting from subdividing some (or all) of its edges. For special subfamilies of these, we are able to describe the limits of their chromatic roots. We also prove that for any ε>0, all sufficiently large subdivisions of G have their chromatic roots in |z−1|<1+ε. A consequence of our work will be a characterization of the graphs having a subdivision whose chromatic polynomial has a root with negative real part

Thermoregulatory capabilities of the woodland dormouse, Graphiurus murinus

The woodland dormouse, Graphiurus murinus, in common with many other small rodents, enters torpor under conditions of food deprivation and low temperatures. Its thermoregulatory capabilities under more favourable conditions, however, have not been investigated. We measured metabolism and thermoregulation in woodland dormice acclimated to long-day length, moderate temperature and abundant food over a temperature range (Ta) of approximately 5–37°C. The thermal neutral zone for this species lay between 29 and 35°C. Estimated resting metabolic rate (RMR) within this range averaged 21.10 ± 3.28 J g-1 h-1. Below 29°C energy expenditure increased with a decrease in Ta, with a maximum value of 90.76 J g-1 h-1 measured at 6°C. Both evaporative water loss (EWL) and thermal conductance (C) were minimal and independent of Tsub&gt;a between 5 and 32°C but increased above the thermal neutral zone, maximum EWL and C being 6.7 mg g-1 h-1 (79% of metabolic heat production) and 19.74 J g-1 h-1 °C-1 respectively, at 37.3°C. Thermal conductance and RMR of G. murinus were approximately 48% and 30% below predicted for rodents of equivalent mass, respectively. This pattern is consistent with that suggested for warm-temperate arboreal rodents. Although body temperature (Tb) was labile and was significantly related to Ta over the temperature range investigated, dormice maintained Tb s between 34 and 38°C using typical thermoregulatory responses that included increased activity at low Ta and postural adjustments and salivating at high Ta. While not as impressive as some rodent species, under favourable conditions G. murinus is a competent thermoregulator and torpor in the species is facultative

Complications in the study of ancient tuberculosis: Presence of environmental bacteria in human archaeological remains

There are many reports of ancient DNA from bacteria of the Mycobacterium tuberculosis complex (MTBC) being present in skeletons with and without osteological indications of tuberculosis. A possible complication in these studies is that extracts might also contain DNA from the microbiome of the individual whose remains are being analysed and/or from environmental bacteria that have colonised the skeleton after death. These contaminants might include ‘mycobacteria other than tuberculosis’ (MOTT), which are common in the environment, but which are not normally associated with clinical cases of tuberculosis. In this paper we show that MOTT of various types, as well as bacteria of related genera, are present in most if not all archaeological remains. Our results emphasise the complications inherent in the biomolecular study of archaeological human tuberculosis. The specificity of any polymerase chain reaction directed at the MTBC cannot be assumed and, to confirm that an amplification is authentic, a sequencing strategy must be applied that allows characterisation of the PCR product. Any variations from the reference MTBC sequence must then be checked against sequence data for MOTT and other species to ensure that the product does actually derive from MTBC. Our results also illustrate the challenges faced when assembling MTBC genome sequences from ancient DNA samples, as misidentification of MOTT sequence reads as MTBC would lead to errors in the assembly. Identifying such errors would be particularly difficult, if not impossible, if the MOTT DNA content is greater than that of the authentic MTBC. The difficulty in identifying and excluding MOTT sequences is exacerbated by the fact that many MOTT are still uncharacterized and hence their sequence features are unknown

Fermi and Gamow-Teller Strength in Charge Exchange with Radioactive Beams

At forward angles, and bombarding energies E > 200 MeV, the (p,n) and (n,p) reactions are thought to be directly proportional to the Gamow-Teller transition strengths in the nuclei. Assuming that this relationship also holds for charge exchange induced by high-energy heavy ions, it would be very useful in studies with radioactive beams. Contrary to this expectation, we show that the determination of Gamow-Teller and Fermi matrix elements from heavy-ion charge-exchange at forward angles is very inaccurate.Comment: 9 pages, RevTeX, 4 PostScript figures available upon reques

Transforming growth factor-beta and endoglin signaling orchestrate wound healing

Physiological wound healing is a complex process requiring the temporal and spatial co-ordination of various signaling networks, biomechanical forces, and biochemical signaling pathways in both hypoxic and non-hypoxic conditions. Although a plethora of factors are required for successful physiological tissue repair, transforming growth factor beta (TGF-β) expression has been demonstrated throughout wound healing and shown to regulate many processes involved in tissue repair, including production of ECM, proteases, protease inhibitors, migration, chemotaxis, and proliferation of macrophages, fibroblasts of the granulation tissue, epithelial and capillary endothelial cells. TGF-β mediates these effects by stimulating signaling pathways through a receptor complex which contains Endoglin. Endoglin is expressed in a broad spectrum of proliferating and stem cells with elevated expression during hypoxia, and regulates important cellular functions such as proliferation and adhesion via Smad signaling. This review focuses on how the TGF-β family and Endoglin, regulate stem cell availability, and modulate cellular behavior within the wound microenvironment, includes current knowledge of the signaling pathways involved, and explores how this information may be applicable to inflammatory and/or angiogenic diseases such as fibrosis, rheumatoid arthritis and metastatic cancer

A single administration of combination therapy inhibits breast tumour progression in bone and modifies both osteoblasts and osteoclasts

We have previously shown that repeated sequential administration of doxorubicin, followed 24 h later by zoledronic acid, inhibits tumour growth in models of established breast cancer bone metastasis. As breast cancer patients only receive zoledronic acid every 3-4 weeks, the aim of the current study was to establish the anti-tumour and bone effects of a single administration of doxorubicin/zoledronic acid combination therapy in a bone metastasis model. MDA-MB-231-GFP cells were injected i.c. in 6-week-old nude mice. On day 2, animals received PBS, doxorubicin (2 mg/kg i.v.), zoledronic acid (100 μg/kg s.c.) or doxorubicin followed 24 h later by zoledronic acid. Anti-tumour effects were assessed on days 15/23 by quantification of apoptotic and proliferating cells and changes in expression of genes implicated in apoptosis, proliferation and bone turnover. Bone effects were assessed by μCT analysis, bone histomorphometry and measurement of serum markers. A tumour-free control group was included. Combination treatment reduced bone tumour burden compared to single agent or PBS control and increased levels of tumour cell apoptosis on day 15, but this was no longer detectable on day 23. Animals receiving zoledronic acid had increased bone density, without evidence of tumour-induced lesions. Bone histomorphometry showed that zoledronic acid caused a decrease in osteoblast and osteoclast numbers and an increase in osteoclast size, in both tumour-free and tumour-bearing animals. Our data show that although zoledronic acid modifies the bone microenvironment through effects on both osteoblasts and osteoclasts, this does not result in a significant anti-tumour effect in the absence of doxorubicin. © 2012 Elsevier GmbH. All rights reserved

The ideological significance of flint in Dynastic Egypt

This thesis examines a little understood aspect of Dynastic Egypt—that of the ideology of flint. Ideology is defined as the way flint is thought of rather than used. This study is unique in examining long term chronological changes in flint ideology against the background of increased metal use, and in using together text, iconography, and archaeology: studies of Egyptian ideology traditionally privilege text. Metaphor theory is employed as an important tool to aid this study. While metaphor is frequently used in Egyptological studies of Egyptian religion, its use is rarely explicit. The dataset brings together unpublished artefacts in British museum collections; a first hand analysis of lithics from Panhesy’s house at Amarna; finds cards from recent excavations at Memphis; and textual sources, several of which have not been considered before in relation to the ideology of flint; as well as published data on Egyptian lithic material. Chronological changes in ideology surrounding flint during the Bronze and Iron Ages, a time of flint decline, are considered. Because the nature of flint decline in Egypt has been assumed rather than known, I attempt to quantify the process. Conclusions show that the ideology of flint was far from static but only loosely related to the kinetic decline of flint. Flint is shown to be connected with the goddesses who are the Eye of Re, with Re himself, with snakes and lions. New facets of flint ideology are uncovered, including the connection of the material with the northern sky and the link between the treatment of New Kingdom Theban flint concretions and the religious landscape of the area

Fibrinogen E fragment selectively disrupts the vasculature and inhibits the growth of tumours in a syngeneic murine model

We recently demonstrated that a fragment of human fibrinogen, fibrinogen E fragment, inhibits the migration and differentiation of human endothelial cells in vitro. Here we show that it exerts similar effects on murine endothelial cells in vitro, and selectively disrupts tumour endothelium in vivo, causing widespread intravascular thrombosis and retarding the growth of CT26 tumours in a syngeneic murine model

Comparison of exact and approximate cross-sections in relativistic Coulomb excitation

We present a new method of obtaining time-dependent matrix elements of the electromagnetic pulse produced by a highly-relativistic projectile. These matrix elements are used in a coupled-channel calculation to predict the cross-sections for population of 1- and 2-phonon states of the giant dipole resonance. Comparisons are made with the predictions of the long-wavelength and Born approximations.Comment: 26 pages, LaTex2