Bemesting biologische fruitteelt

Deze brochure bevat resultaten en aanbevelingen afkomstig uit het ‘Bedrijfsnetwerk Biologische Fruitteelt’. Behandeld worden oa.: bodemkwaliteit, voedsel voor bomen, wetgeving en strategie

Kleinfruit klimaatneutraal telen: Bemesting en diesel leveren grootste bijdrage aan de carbon footprint van bio kleinfruittelers.

Zes biologische kleinfruittelers onderzochten in de afgelopen twee jaar de mogelijkheden van klimaatneutraal telen. Bemesting en dieselgebruik blijken op hun bedrijven een belangrijk bijdrage te leveren aan de carbonfootprint, terwijl elektriciteit nauwelijks een rol speelt. De ervaringen zijn gebundeld als Best Practices

Informatie en literatuur voor de biologische fruitteelt : Information and literature for organic fruit growing

Informatie over organisaties voor de biologische fruitteelt in diverse landen. Verwijzingen naar onderzoek

How to optimize fruit and berry cultivar selection for organic farmers ? A comparison of European approaches.

Most of the currently grown commercial fruit cultivars remain unsuitable to low-input or sustainable organic farming systems. The major reason is that they have been developed and selected under high input cultivation, and without sufficient attention to susceptibility to pests and diseases. Some of these cultivars are nevertheless marketed as resistant because they carry genetic markers for major resistance genes but these are unfortunately prone to break-down (most of apple resistant cultivars relay only on the Vf). As a result, new fruit cultivars tend to reveal unexpected and often undesirable traits when grown in commercial organic orchards, and thus fail to bring about the much needed expansion in organic production and market availability. However, research is ongoing, and some institutions in Europe have established specific approaches in their breeding programs for testing new selections and cultivars under entirely organic conditions – sometimes including also evaluation of storage capability, consumer acceptance or suitable characteristics for juice and cider processing. This paper gives an overview of approaches developed in some European countries, their characteristics, objectives and results. It shows a number of different ways to address a difficult topic. We conclude that it is very desirable to – on a European level – exchange results and ideas to further improve the testing systems, and to increase the distribution of promising cultivars for the European organic fruit sector

Involvement of MicroRNAs in the Aging-Related Decline of CD28 Expression by Human T Cells

Loss of CD28 is a characteristic feature of T cell aging, but the underlying mechanisms of this loss are elusive. As differential expression of microRNAs (miRNAs) has been described between CD28+ and CD28- T cells, we hypothesized that altered miRNA expression contributes to the age-associated downregulation of CD28. To avoid the confounding effects of age-associated changes in the proportions of T cells at various differentiation stages in vivo, an experimental model system was used to study changes over time in the expression of miRNA associated with the loss of CD28 expression in monoclonal T cell populations at a lower or higher number of population doublings (PDs). This approach allows identification of age-associated miRNA expression changes in a longitudinal model. Results were validated in ex vivo samples. The cumulative number of PDs but not the age of the donor of the T cell clone was correlated with decreased expression of CD28. Principal component analysis of 252 expressed miRNAs showed clustering based on low and high PDs, irrespective of the age of the clone donor. Increased expression of miR-9-5p and miR-34a-5p was seen in clones at higher PDs, and miR-9-5p expression inversely correlated with CD28 expression in ex vivo sorted T-cells from healthy subjects. We then examined the involvement of miR-9-5p, miR-34a-5p, and the members of the miR-23a similar to 24-2 cluster, in which all are predicted to bind to the 3'UTR of CD28, in the IL-15-induced loss of CD28 in T cells. Culture of fresh naive CD28+ T cells in the presence of IL-15 resulted in a gradual loss of CD28 expression, while the expression of miR-9-5p, miR-34a-5p, and members of the miR-23a-24-2 cluster increased. Binding of miR-9-5p, miR-34a-5p, miR-24-3p, and miR-27- 3p to the 3'UTR of CD28 was studied using luciferase reporter constructs. Functional binding to the 3'UTR was shown for miR-24-3p and miR-27a-3p. Our results indicate involvement of defined miRNAs in T cells in relation to specific characteristics of T cell aging, i.e., PD and CD28 expression

Knowledge Networks in Organic Fruit Production across Europe: A Survey Study

Limited data regarding the resources and methods used by organic fruit growers to learn about production practices are available, even though this information is crucial to improving the efficacy of knowledge transfer. Therefore, a survey to gain information from knowledge networks dealing with organic fruit production about their structural organisation, tasks and methods of communication was carried out in twenty-one countries from Europe and the Mediterranean basin. A total of 56 networks representing about 42,500 professionals were identified as a result of the survey. The vast majority of them were only active at the regional or national level and were composed of farmers, advisors and researchers. About 3/4 of the networks were developing improved strategies for agronomic practices and about half of them were also involved in different knowledge-transfer activities between their members. Personal contact was the most used method to exchange and disseminate information within the networks as well as to elaborate improved strategies. The findings were analysed in view of the methods and practices commonly used to share both explicit (scientific) and implicit (practical) knowledge among practitioners. It was concluded that knowledge networks play an important role in the development of more resilient organic cropping systems, frequently making organic fruit growers the drivers of innovation. Networking for knowledge exchange was considered a process that encourages the active involvement of farmers in experimentation and innovation applying a method of knowledge sharing that is rooted in the very foundation of organic philosophy. Some recommendations and future research were suggested to further foster the development and functioning of networks for knowledge exchange

Preliminary report on osteochondrosis in cattle in the north-western parts of South Africa

The north-western part of South Africa, in particular, is well known for mineral imbalances. Aphosphorosis, resulting in rickets and osteomalacia, received a lot of attention at the turn of the nineteenth century (1882–1912). This was followed in 1997 by research on Vryburg hepatosis, another area-specific mineral imbalance–related disease in young calves reared on manganese-rich soil derived from the weathering of dolomitic (carbonate) rock formations. In 1982, a totally new syndrome (osteochondrosis) manifested in, amongst others, areas in South Africa where aphosphorosis was rife. Osteochondrosis was also identified in the south-western parts of Namibia as well as southern Botswana and other areas in South Africa. Osteochondrosis has a multifactorial aetiology and this study focused on the role of minerals, particularly phosphorus, in the development of the disease. A significant improvement in the clinical signs in experimental animals and a reduction of osteochondrosis occurred on farms where animals received bioavailable trace minerals and phosphorus as part of a balanced lick. An increase in the occurrence of the disease on farms during severe drought conditions in 2012–2013 prompted researchers to investigate the possible role of chronic metabolic acidosis in the pathogenesis of the disease.http://www.ojvr.org/am2016Paraclinical Science

Mutations in CYB561 Causing a Novel Orthostatic Hypotension Syndrome

Rationale: Orthostatic hypotension is a common clinical problem, but the underlying mechanisms have not been fully delineated. Objective: We describe two families, with four patients in total, suffering from severe life-threatening orthostatic hypotension due to a novel cause. Methods and Results: As in dopamine β-hydroxylase deficiency (DβH), concentrations of norepinephrine and epinephrine in the patients were very low. Plasma DβH activity, however, was normal and the DBH gene had no mutations. Molecular genetic analysis was performed to determine the underlying genetic cause. Homozygosity mapping and exome and Sanger sequencing revealed pathogenic homozygous mutations in the gene encoding cytochrome b561 (CYB561); a missense variant c.262G>A, p.Gly88Arg in exon 3 in the Dutch family and a nonsense mutation (c.131G>A, p.Trp44*) in exon 2 in the American family. Expression of CYB561 was investigated using RNA from different human adult and fetal tissues, transcription of RNA into cDNA and real-time quantitative polymerase chain reaction. The CYB561 gene was found to be expressed in many human tissues, in particular the brain. The CYB561 protein defect leads to a shortage of ascorbate inside the catecholamine secretory vesicles leading to a functional DβH deficiency. The concentration of the catecholamines and downstream metabolites was measured in brain and adrenal tissue of six CYB561 knockout mice (reporter-tagged deletion allele (post-Cre), genetic background C57BL/6NTac). The concentration of norepinephrine and normetanephrine was decreased in whole brain homogenates of the CYB561(-/-) mice compared to wild type mice (p<0.01) and the concentration of normetanephrine and metanephrine was decreased in adrenal glands (p<0.01), recapitulating the clinical phenotype. The patients responded favorably to treatment with L-dihydroxyphenylserine, which can be converted directly to norepinephrine. Conclusions: This study is the first to implicate cytochrome b561 in disease by showing that pathogenic mutations in CYB561 cause an as yet unknown disease in neurotransmitter metabolism causing orthostatic hypotension. as yet unknown disease in neurotransmitter metabolism causing orthostatic hypotension

Using a genetic, observational study as a strategy to estimate the potential cost-effectiveness of pharmacological CCR5 blockade in dialysis patients

Clinical epidemiolog

Susceptibility to chronic mucus hypersecretion, a genome wide association study

Background: Chronic mucus hypersecretion (CMH) is associated with an increased frequency of respiratory infections, excess lung function decline, and increased hospitalisation and mortality rates in the general population. It is associated with smoking, but it is unknown why only a minority of smokers develops CMH. A plausible explanation for this phenomenon is a predisposing genetic constitution. Therefore, we performed a genome wide association (GWA) study of CMH in Caucasian populations. Methods: GWA analysis was performed in the NELSON-study using the Illumina 610 array, followed by replication and meta-analysis in 11 additional cohorts. In total 2,704 subjects with, and 7,624 subjects without CMH were included, all current or former heavy smokers (≥20 pack-years). Additional studies were performed to test the functional relevance of the most significant single nucleotide polymorphism (SNP). Results: A strong association with CMH, consistent across all cohorts, was observed with rs6577641 (p = 4.25x10-6, OR = 1.17), located in intron 9 of the special AT-rich sequence-binding protein 1 locus (SATB1) on chromosome 3. The risk allele (G) was associated with higher mRNA expression of SATB1 (4.3x10 -9) in lung tissue. Presence of CMH was associated with increased SATB1 mRNA expression in bronchial biopsies from COPD patients. SATB1 expression was induced during differentiation of primary human bronchial epithelial cells in culture. Conclusions: Our findings, that SNP rs6577641 is associated with CMH in multiple cohorts and is a cis-eQTL for SATB1, together with our additional observation that SATB1 expression increases during epithelial differentiation provide suggestive evidence that SATB1 is a gene that affects CMH