TOXOPLASMA GONDII NEGLI UNGULATI SELVATICI VIVENTI IN TOSCANA:INDAGINI SIEROLOGICA E MOLECOLARE

rilevazione toxoplasma gondi

Analisi multivariata per osservazioni appaiate con dati mancanti: un caso studio

All parametric approaches require that analysis should be done on complete data sets and so, in presence of missing data, parametric solutions are based either on the so-called deletion principle or imputation methods. But when we delete incomplete vectors we also remove all information they contain, which may be valuable and useful for analysis. And when we replace missing data by suitable functions of actually observed data, that is imputing method, we may introduce biased information which may negatively infuence the analysis. On the other hand, non-parametric solutions in a permutation framework consider data as they are, and units with missing data participate in the permutation mechanism as well as all other units, without deletion or imputing. In this paper we provide a comparison between a parametric solution, represented by ITT principle, and a non parametric one, in a testing problem with multivariate paired observations

Prevalenza stagionale di specie micotiche nella congiuntiva di bovini sani: risultati conseguiti nel biennio 2006-2008

Lo studio è stato condotto in tre allevamenti,a diversa conduzione, della provincia di Pisa. Da ciascun allevamento sono stati presi come campione, nel periodo che parte da novembre 2006 e arriva ad agosto 2008,venti capi bovini,rimasti tali nel corso di questi anni. A ciascun soggetto è stato praticato un campione oculare(ad entrambi gli occhi)e,nello stesso giorno è stata campionata l’aria tramite RODAC e l’alimento,tramite procedure effettuate in laboratorio. Il campionamento di aria ed alimento è stato eseguito per verificare se vi è correlazione tra le muffe ed i lieviti riscontrati nel campione oculare;la scelta invece degli allevamenti a diversa conduzione si è resa importante per verificare se l’ambiente e la gestione possa influire sui risultati ottenuti

Aldh2 is a lineage-specific metabolic gatekeeper in melanocyte stem cells

Melanocyte stem cells (McSCs) in zebrafish serve as an on-demand source of melanocytes during growth and regeneration, but metabolic programs associated with their activation and regenerative processes are not well known. Here, using live imaging coupled with scRNA-sequencing, we discovered that, during regeneration, quiescent McSCs activate a dormant embryonic neural crest transcriptional program followed by an aldehyde dehydrogenase (Aldh) 2 metabolic switch to generate progeny. Unexpectedly, although ALDH2 is well known for its aldehyde-clearing mechanisms, we find that, in regenerating McSCs, Aldh2 activity is required to generate formate – the one-carbon (1C) building block for nucleotide biosynthesis – through formaldehyde metabolism. Consequently, we find that disrupting the 1C cycle with low doses of methotrexate causes melanocyte regeneration defects. In the absence of Aldh2, we find that purines are the metabolic end product sufficient for activated McSCs to generate progeny. Together, our work reveals McSCs undergo a two-step cell state transition during regeneration, and that the reaction products of Aldh2 enzymes have tissue-specific stem cell functions that meet metabolic demands in regeneration

Laryngeal mask airway in neonatal stabilization and transport: a retrospective study.

Laryngeal mask airway (LMA) may be considered by health caregivers of level I–II hospitals for neonatal resuscitation and stabilization before and during interhospital care, but literature provides little information on this aspect. This study reviewed the use of LMA during stabilization and transport in a large series of neonates. This is a retrospective study evaluating the use of LMA in infants who underwent emergency transport by the Eastern Veneto Neonatal Emergency Transport Service between January 2003 and December 2021. All data were obtained from transport registry, transport forms, and hospital charts. In total, 64/3252 transferred neonates (2%) received positive pressure ventilation with an LMA, with increasing trend over time (p = 0.001). Most of these neonates were transferred after birth (97%), due to a respiratory or neurologic disease (95%). LMA was used before the transport (n = 60), during the transport (n = 1), or both (n = 3). No device-related adverse effects were recorded. Sixty-one neonates (95%) survived and were discharged/transferred from the receiving center

Origin of fluoride and arsenic in the main ethiopian rift waters

In the Main Ethiopian Rift (MER) area, rural populations often use water that exceeds the World Health Organization thresholds for fluoride (F–) and arsenic (As), two elements that are hazardous for human health. In this study, twenty-nine water samples were collected from lakes and hot and cold springs in southern MER to investigate source(s) and health-risk of the F– and As contamination. According to major ion and trace element analyses, only cold spring water is safe for consumption, whereas hot spring water is the most contaminated. Leaching tests performed with the MER rhyolitic volcanic rocks and their weathered products (fluvio-lacustrine sediments) demonstrate that the main cause of the F– and As release is geogenic, i.e., not related to anthropogenic activities. The weathering of volcanic glass and minerals (apatites, clays, hydro-oxides) by CO2-bearing alkaline water induces the mobilisation of F– and As from solid to liquid phase. This process is particularly fast, when fluvio-lacustrine sediments are involved, and can be further enhanced by hot groundwater leaching. This study, investigating the distribution, sources, and mechanisms of F– and As release in MER water, could be of interest also for other sectors of the East African Rift and other similar volcano-tectonic settings

Beamlet scraping and its influence on the beam divergence at the BATMAN Upgrade test facility

For the ITER fusion experiment, two neutral beam injectors are required for plasma heating and current drive. Each injector supplies a power of about 17 MW, obtained from neutralization of 40 A (46 A), 1 MeV (0.87 MeV) negative deuterium (hydrogen) ions. The full beam is composed of 1280 beamlets, formed in 16 beamlet groups, and strict requirements apply to the beamlet core divergence (<7 mrad). The test facility BATMAN Upgrade uses an ITER-like grid with one beamlet group, which consists of 70 apertures. In a joint campaign performed by IPP and Consorzio RFX to better assess the beam optics, the divergence of a single beamlet was compared to a group of beamlets at BATMAN Upgrade. The single beamlet is measured with a carbon fiber composite tile calorimeter and by beam emission spectroscopy, whereas the divergence of the group of beamlets is measured by beam emission spectroscopy only. When increasing the RF power at low extraction voltages, the divergence of the beamlet and of the group of beamlets is continuously decreasing and no inflection point toward an overperveant beam is found. At the same time, scraping of the extracted ion beam at the second grid (extraction grid) takes place at higher RF power, supported by the absence of the normally seen linear behavior between the measured negative ion density in the plasma close to the extraction system and the measured extracted ion current. Beside its influence on the divergence, beamlet scraping needs to be considered for the determination of the correct perveance and contributes to the measured coextracted electron current

Physiological Response to Negative Emotions in Children with Anxiety Symptoms: Many Steps Still to be Taken

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Peat soil burning in the Mezzano lowland (Po Plain, Italy): triggering mechanisms and environmental consequences.

The effects of peat burning on organic-rich agricultural soils of the Mezzano Lowland (NE Italy) were evaluated on soil profiles variously affected by smoldering. Profiles were investigated for pH, electrical conductivity, bulk density, elemental and isotopic composition of distinct carbon (and nitrogen) fractions. The results suggest that the horizons affected by carbon loss lie at depths 10–70 cm, where the highest temperatures are developed. We suggest that the exothermal oxidation of methane (mediated by biological activity) plays a significant role in the triggering mechanism. In the interested soils we estimated a potential loss of Soil Organic Carbon of approximately 110 kg m−2 within the first meter, corresponding to 580 kg CO2 m−3. The released greenhouse gas is coupled with a loss of soil structure and nutrients. Moreover, the process plausibly triggers mobility of metals bound in organometallic complexes. All these consequences negatively affect the environment, the agricultural activities and possibly also health of the local people

Chemical characterisation of construction and demolitionwaste in Skopje city and its surroundings (Republic of Macedonia)

In the Republic of Macedonia, construction and demolition waste is often dumped, underestimating the potential recycling and re-use as raw materials for civil engineering works and/or cement/ceramic industries. SAMCODE (Sustainable Approach to Managing Construction and Demolition Waste) is a know-how exchange program, the focus of which is chemical characterisation in terms of major and trace elements in order to evaluate the possible Macedonian construction and demolition waste recycling. Thirty-nine waste samples were collected from different dumps in Skopje and surroundings. X-ray fluorescence analyses, carried out on powdered samples, show i) highly variable concentrations, indicative of the heterogenous nature of construction and demolition waste, and ii) high concentration in Cr, Ni, and Zn with respect to Italian, Chinese, and Dutch tolerance limits, probably due to the presence of these elements in ophiolitic rocks and sulphide-bearing deposits, used as raw material in building activity. Inductively coupled plasma mass spectrometry analyses of leachates, performed to assess the mobility of heavy metals, show significant concentrations of Cr, and to a lesser extent, Ni. Results suggest that homogenisation processes of the recycled materials should be implemented and preliminary screening of construction and demolition waste should be performed to eliminate heavy metals-bearing components