1,272 research outputs found
Food Habits of the Hoary Bat in an Agricultural Landscape
Information on diets is fundamental to ecological studies. Prey use by the solitary, tree-roosting hoary bat (Lasiurus cinereus) in agricultural landscapes is not known. We examined the stomach contents and fecal material from carcasses of hoary bats collected during a mortality study at wind turbine sites in southwestern Minnesota. We compared diet of hoary bats to availability of prey to determine whether bats were opportunistic or selective. Food of the hoary bats primarily consisted of lepidopterans (moths; 49-50 %) and coleopterans (beetles; 28-40 %). The abundance of insects in the diet of hoary bats was not proportional to the estimated availability of prey. Hoary bats selected large, soft-bodied insects (e.g., lepidopterans and neuropterans) and avoided small or hard-bodied insects (e.g., coleopterans, dipterans, and hemipterans). We suggest that hoary bats do not select prey based on availability, but rather, select prey that are large and soft-bodied
Food Habits of Dabbling Ducks During Fall Migration in a Prairie Pothole System, Heron Lake, Minnesota
We conducted an analysis of dabbling duck food habits in the fall of 2002 and 2003 in the Heron Lake system. Gizzard contents of hunter-harvested birds were analyzed using the percent aggregate volume method to determine what food items were consumed and in what quantity. Curltop Ladysthumb (Polygonum lapathifolium) was the food item consumed most often (82.2%) and in the greatest volume (34.2 ml). Sago Pondweed (Stuckenia pectinata) was the only food item of which multiple plant parts were consumed. However, the seeds and tubers only comprised 1.27 and 0.07 of the total aggregate percent
Life History and Habitat of the Rare Patch-nosed Salamander (Urspelerpes brucei)
We examined the life history and habitat characteristics for the Patch-nosed Salamander, Urspelerpes brucei. Body-size measurements of individuals captured using litter bags and by hand from 2008 to 2010 indicated that the larval period lasts at least 2 y, salamanders attain reproductive maturity at or shortly after metamorphosis, and adults have very little variation in body size. Occupied streams are characterized by small size, little water, and narrow, steep-walled ravines. Within occupied streams, larval capture rate was significantly and negatively related to mean water depth, underscoring the importance of protecting headwaters. We hypothesize that the only known population of U. brucei east of the Tugaloo River was isolated from the west-bank populations by the tremendous increase in w
The Sorghum bicolor reference genome: improved assembly, gene annotations, a transcriptome atlas, and signatures of genome organization.
Sorghum bicolor is a drought tolerant C4 grass used for the production of grain, forage, sugar, and lignocellulosic biomass and a genetic model for C4 grasses due to its relatively small genome (approximately 800 Mbp), diploid genetics, diverse germplasm, and colinearity with other C4 grass genomes. In this study, deep sequencing, genetic linkage analysis, and transcriptome data were used to produce and annotate a high-quality reference genome sequence. Reference genome sequence order was improved, 29.6 Mbp of additional sequence was incorporated, the number of genes annotated increased 24% to 34 211, average gene length and N50 increased, and error frequency was reduced 10-fold to 1 per 100 kbp. Subtelomeric repeats with characteristics of Tandem Repeats in Miniature (TRIM) elements were identified at the termini of most chromosomes. Nucleosome occupancy predictions identified nucleosomes positioned immediately downstream of transcription start sites and at different densities across chromosomes. Alignment of more than 50 resequenced genomes from diverse sorghum genotypes to the reference genome identified approximately 7.4 M single nucleotide polymorphisms (SNPs) and 1.9 M indels. Large-scale variant features in euchromatin were identified with periodicities of approximately 25 kbp. A transcriptome atlas of gene expression was constructed from 47 RNA-seq profiles of growing and developed tissues of the major plant organs (roots, leaves, stems, panicles, and seed) collected during the juvenile, vegetative and reproductive phases. Analysis of the transcriptome data indicated that tissue type and protein kinase expression had large influences on transcriptional profile clustering. The updated assembly, annotation, and transcriptome data represent a resource for C4 grass research and crop improvement
Mitotic stress is an integral part of the oncogene-induced senescence program that promotes multinucleation and cell cycle arrest
Oncogene-induced senescence (OIS) is a tumor suppression mechanism that blocks cell proliferation in response to oncogenic signaling. OIS is frequently accompanied by multinucleation; however, the origin of this is unknown. Here, we show that multinucleate OIS cells originate mostly from failed mitosis. Prior to senescence, mutant H-RasV12 activation in primary human fibroblasts compromised mitosis, concordant with abnormal expression of mitotic genes functionally linked to the observed mitotic spindle and chromatin defects. Simultaneously, H-RasV12 activation enhanced survival of cells with damaged mitoses, culminating in extended mitotic arrest and aberrant exit from mitosis via mitotic slippage. ERK-dependent transcriptional upregulation of Mcl1 was, at least in part, responsible for enhanced survival and slippage of cells with mitotic defects. Importantly, mitotic slippage and oncogene signaling cooperatively induced senescence and key senescence effectors p21 and p16. In summary, activated Ras coordinately triggers mitotic disruption and enhanced cell survival to promote formation of multinucleate senescent cells
OREXIN 1 AND 2 RECEPTOR INVOLVEMENT IN CO2 -INDUCED PANIC-ASSOCIATED BEHAVIOR AND AUTONOMIC RESPONSES
BACKGROUND:
The neuropeptides orexin A and B play a role in reward and feeding and are critical for arousal. However, it was not initially appreciated that most prepro-orexin synthesizing neurons are almost exclusively concentrated in the perifornical hypothalamus, which when stimulated elicits panic-associated behavior and cardiovascular responses in rodents and self-reported "panic attacks" and "fear of dying" in humans. More recent studies support a role for the orexin system in coordinating an integrative stress response. For instance, orexin neurons are highly reactive to anxiogenic stimuli, are hyperactive in anxiety pathology, and have strong projections to anxiety and panic-associated circuitry. Although the two cognate orexin receptors are colocalized in many brain regions, the orexin 2 receptor (OX2R) most robustly maps to the histaminergic wake-promoting region, while the orexin 1 receptor (OX1R) distribution is more exclusive and dense in anxiety and panic circuitry regions, such as the locus ceruleus. Overall, this suggests that OX1Rs play a critical role in mobilizing anxiety and panic responses.
METHODS:
Here, we used a CO2 -panic provocation model to screen a dual OX1/2R antagonist (DORA-12) to globally inhibit orexin activity, then a highly selective OX1R antagonist (SORA1, Compound 56) or OX2R antagonist (SORA2, JnJ10397049) to assess OX1R and OX2R involvement.
RESULTS:
All compounds except the SORA2 attenuated CO2 -induced anxiety-like behaviors, and all but the SORA2 and DORA attenuated CO2 -induced cardiovascular responses.
CONCLUSIONS:
SORA1s may represent a novel method of treating anxiety disorders, with no apparent sedative effects that were present with a benzodiazepine
CONSTANS is a photoperiod regulated activator of flowering in sorghum
BACKGROUND: Sorghum genotypes used for grain production in temperate regions are photoperiod insensitive and flower early avoiding adverse environments during the reproductive phase. In contrast, energy sorghum hybrids are highly photoperiod sensitive with extended vegetative phases in long days, resulting in enhanced biomass accumulation. SbPRR37 and SbGHD7 contribute to photoperiod sensitivity in sorghum by repressing expression of SbEHD1 and FT-like genes, thereby delaying flowering in long days with minimal influence in short days (PNAS_108:16469-16474, 2011; Plant Genome_in press, 2014). The GIGANTEA (GI)-CONSTANS (CO)-FLOWERING LOCUS T (FT) pathway regulates flowering time in Arabidopsis and the grasses (J Exp Bot_62:2453-2463, 2011). In long day flowering plants, such as Arabidopsis and barley, CONSTANS activates FT expression and flowering in long days. In rice, a short day flowering plant, Hd1, the ortholog of CONSTANS, activates flowering in short days and represses flowering in long days. RESULTS: Quantitative trait loci (QTL) that modify flowering time in sorghum were identified by screening Recombinant Inbred Lines (RILs) derived from BTx642 and Tx7000 in long days, short days, and under field conditions. Analysis of the flowering time QTL on SBI-10 revealed that BTx642 encodes a recessive CONSTANS allele containing a His106Tyr substitution in B-box 2 known to inactivate CONSTANS in Arabidopsis thaliana. Genetic analysis characterized sorghum CONSTANS as a floral activator that promotes flowering by inducing the expression of EARLY HEADING DATE 1 (SbEHD1) and sorghum orthologs of the maize FT genes ZCN8 (SbCN8) and ZCN12 (SbCN12). The floral repressor PSEUDORESPONSE REGULATOR PROTEIN 37 (PRR37) inhibits sorghum CONSTANS activity and flowering in long days. CONCLUSION: Sorghum CONSTANS is an activator of flowering that is repressed post-transcriptionally in long days by the floral inhibitor PRR37, contributing to photoperiod sensitive flowering in Sorghum bicolor, a short day plant
Histone chaperone HIRA deposits histone H3.3 onto foreign viral DNA and contributes to anti-viral intrinsic immunity
The HIRA histone chaperone complex deposits histone H3.3 into nucleosomes in a DNA replication- and sequence-independent manner. As herpesvirus genomes enter the nucleus as naked DNA, we asked whether the HIRA chaperone complex affects herpesvirus infection. After infection of primary cells with HSV or CMV, or transient transfection with naked plasmid DNA, HIRA re-localizes to PML bodies, sites of cellular anti-viral activity. HIRA co-localizes with viral genomes, binds to incoming viral and plasmid DNAs and deposits histone H3.3 onto these. Anti-viral interferons (IFN) specifically induce HIRA/PML co-localization at PML nuclear bodies and HIRA recruitment to IFN target genes, although HIRA is not required for IFN-inducible expression of these genes. HIRA is, however, required for suppression of viral gene expression, virus replication and lytic infection and restricts murine CMV replication in vivo. We propose that the HIRA chaperone complex represses incoming naked viral DNAs through chromatinization as part of intrinsic cellular immunity
Multivariate discrimination and the Higgs + W/Z search
A systematic method for optimizing multivariate discriminants is developed
and applied to the important example of a light Higgs boson search at the
Tevatron and the LHC. The Significance Improvement Characteristic (SIC),
defined as the signal efficiency of a cut or multivariate discriminant divided
by the square root of the background efficiency, is shown to be an extremely
powerful visualization tool. SIC curves demonstrate numerical instabilities in
the multivariate discriminants, show convergence as the number of variables is
increased, and display the sensitivity to the optimal cut values. For our
application, we concentrate on Higgs boson production in association with a W
or Z boson with H -> bb and compare to the irreducible standard model
background, Z/W + bb. We explore thousands of experimentally motivated,
physically motivated, and unmotivated single variable discriminants. Along with
the standard kinematic variables, a number of new ones, such as twist, are
described which should have applicability to many processes. We find that some
single variables, such as the pull angle, are weak discriminants, but when
combined with others they provide important marginal improvement. We also find
that multiple Higgs boson-candidate mass measures, such as from mild and
aggressively trimmed jets, when combined may provide additional discriminating
power. Comparing the significance improvement from our variables to those used
in recent CDF and DZero searches, we find that a 10-20% improvement in
significance against Z/W + bb is possible. Our analysis also suggests that the
H + W/Z channel with H -> bb is also viable at the LHC, without requiring a
hard cut on the W/Z transverse momentum.Comment: 41 pages, 5 tables, 29 figure
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