Antifungal activity of ethanolic extract of Phlomis fruticosa L.

In the ethanolic extract of Phlomis fruticosa L. two flavonoid aglycones, luteolin and chrysoeriol were identified. Antifungal activity of the total ethanolic extract, extract hydrolyzed with β-glucosidase and that hydro-lyzed with HCI against seven micromycetes: Aspergillus niger, A. ochraceus, Penicillium ochrochloron, Cladosporium cladosporioides, Thchoderma viride, Fusamim tricinctum and Phomopsis helianthi was tested. The ethanolic extract hydrolyzed with HCI possessed the greatest antifungal activity against all fungal species tested.U etanolnom ekstraktu vrste Phlomis fruticosa L. identifikovana su dva aglikona flavonoida, luteolin i hrizoeriol. Antifungaliia aktivnost ukupnog etapolnogekstrakta, etanolpog ekstrakta hidrolizovapog β-glukozidazom i ekstrakta hidrolizovanog hlorovodoničnom kiselinom istraživana je na sedam mikromiceta: Aspergillus niger, A. ochraceus, Penicillium ochrochloron, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum i Phomopsis helianthi. Najosetljivije mikromicete cy P. helianthi i C. cladosporioides. Pomenute vrste pokazale su najmanju rezistentnost na sva tri ispitivana ekstrakta. Mikromicete koje su pokazale najveću rezistentnost na sva tri ispitivana ekstrakta su P. ochrochloron i T. viride. Ukupni etanolni ekstrakt pokazao je najmanji antifungalni potencijal. Etanolni ekstakt hidrolizovan enzimom pokazuje porast antifungalnog potencijala, dok etanolni ekstrakt hidrolizovan hlorovodoničnom kiselinom poseduje najveći antifugalni potencijal.Projekat ministarstva br. 03E08/1, 03E2

The rehabilitation of the experimental brain injury with hyperbaric oxygenation

Uvod: Traumatske povrede centralnog nervnog sistema (CNS) predstavljaju širom sveta veliki zdravstveni problem, jer spadaju među vodeće uzročnike smrtnosti i različitih oblika invaliditeta u radno sposobnoj populaciji. Činjenica je da se samo u koordinisanim istraživanjima kako eksperimentalnim i tako kliničnim, mogu razviti novi kolaborativni modeli i nove strategije za sanaciju posledica moždane traume. U ovom kontekstu hiperbarična oksigenacija (HBO) se izdvaja kako procedura koja može u sinergističkom dejstvu sa drugim terapijama dovesti do ubrzanja oporavka posle moždane traume. Cilj ove studije je da utvrdi da li primena HBO može poboljšati oporavak pacova posle sukcione ablacije desnog senzomotornog korteksa. Materijal i metode: Deset nedelja stari mužijaci pacova Wistar soja raspoređeni su u sledeće grupe (n=8, po grupi): Kontrolna (K) intakne životinje, Kontrolna + HBO (KHBO) intakne životinje koje su prošle kroz HBO protokol, Šam (Š) lažno operisane životinje, Šam + HBO (ŠHBO), Lezija (L) desni sezomotorni korteks je uklonjen sukcionom ablacijom i L + HBO (LHBO). HBO protokol: pritisak 2,5 apsolutne atmosfere, 60 minuta, jedanput dnevno, tokom 10 dana. Za evaluaciju procesa oporavka lokomotornih funkcija korišćeni su test prelaska preko grede i merenje snage stiska. Količina malondialdehida (MDA), aktivnost superoksid dismutaze (SOD) i glutation peroksidaze (GPX) su mereni u uzorcima tkiva povređenog hemikorteksa. Postraumatski procesi su praćeni na uzorcima moždanog tkiva imunohistohemijskom metodom sa sledećim antitelima: GFAP, ED1, SMI31, GAP43 i SYP. U cilju evaluacije efekata HBO na proces neuronalne degeneracije sprovedeno je dovostruko imunofluorescentno bojenje: neuroni u procesu degeneracije su bojeni fluoro žadom B, dok je NeuN korišćen kao marker tela neurona...Objectives: Traumatic brain injuries (TBI) are one of the leading causes of death and chronic disability especially among the working population, and represent an important public health problem worldwide. It is proposed that coordinated research efforts of basic and clinical scientists will provide the opportunity to develop collaborative models for translational neuroscience and rehabilitation research. In this view hyperbaric oxygen therapy (HBO) appeared as an adjunctive therapy that has the synergistic effect with other treatments, strongly suggesting that combining therapies with HBO could yield better results than either alone. The aim of this study was to investigate whether HBO can improve the neurological recovery of the rat after suction ablation of the sensorimotor cortex. Methods: The experiments were conducted on the male Wister rats, 10 weeks old. Animals were organized into following groups (n = 8, per group): Control (C) intact animals, Control+HBO (CHBO) intact animals subjected to HBO treatment, Sham control (S) animals that underwent surgical procedure without damaging the brain tissue, Sham control+HBO (SHBO), Lesion group (L)–the right sensorimotor cortex was removed by suction ablation, Lesion+HBO (LHBO). HBO protocol: pressure applied 2.5 absolute atmospheres (ATA), for 60 minutes, once daily for 10 days. Beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Malondialdehyde (MDA) content, activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) sampled from injured hemicortex were measured. The posttraumatic processes were evaluated on the brain tissue preparations using the immunohistochemical method with antibodies: GFAP, ED1, SMI31, GAP43 and SYP..

Streamlining collection of training samples for object detection and classification in video

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Combination free replacement and pro-rata warranty policy optimization model

Rizik koji prati razvoj proizvoda raste iz dana u dan. Jedan od faktora koji utiču na ovaj rizik je garancija proizvoda. Garancija je moćno marketinško oružje za proizvođača i istovremeno dobra zaštita za proizvođača i kupca, ali uvek podrazumeva dodatne troškove za proizvođača. Ovi troškovi zavise od karakteristika pouzdanosti proizvoda i parametara garancije. Ovaj rad se bavi optimizacijom ovih parametara za poznatu raspodelu otkaza proizvoda u cilju smanjivanja troškova garancije i istovremenog zadržavanja njene promotivne funkcije. Kombinacija garancije besplatne zamene i parcijalne garancije je izabrana kao model, pri čemu su varirane dužine perioda besplatne zamene i perioda parcijalne garancije, kao i koeficijenti koji definišu funkciju parcijalnih troškova. Vrednosti troškova garancije dobijene su pomoću analitičkih jednačina i simulacije. Dobijeni rezultati su prikazani i razmotreni i izneta su zaključna opažanja.Product development risk increases more and more every day. One of the factors that affect this risk is product warranty. Warranty is a powerful marketing instrument for the manufacturer and a good protection for both the manufacturer and the customer, but it always involves additional costs to the manufacturer. These costs depend on the product reliability and the warranty parameters. This paper deals with the optimization of these parameters for known product failure distribution to reduce the warranty costs to the manufacturer while retaining the promotional function of the warranty. Combination free replacement and pro-rata warranty policy is chosen as a model and the length of free replacement and pro-rata policy periods are varied, as well as the coefficients that define the pro-rata cost function. Warranty costs are obtained by using analytical equations and by simulation. The obtained results are shown and discussed and some concluding remarks are given

High fidelity simulations of ion trajectories in miniature ion traps using the boundary-element method

In this paper we present numerical modeling results for endcap and linear ion traps, used for experiments at the National Physical Laboratory in the UK and Innsbruck University respectively. The secular frequencies for Strontium-88 and Calcium-40 ions were calculated from ion trajectories, simulated using boundary-element and finite-difference numerical methods. The results were compared against experimental measurements. Both numerical methods showed high accuracy with boundary-element method being more accurate. Such simulations can be useful tools for designing new traps and trap arrays. They can also be used for obtaining precise trapping parameters for desired ion control when no analytical approach is possible as well as for investigating the ion heating rates due to thermal electronic noise.Comment: 6 pages, 5 figures, changes made to the text according to the editor's and referee's comment

Risk Model for Integrated Management System

The purpose of this paper is to develop an integrated risk management model for standardized management systems: ISO 9001:2015 for quality management, ISO 14001:2015 for environmental management, ISO/IEC 27001:2013 for information security management, ISO 45001:2018 for Occupational Health and Safety Management, and ISO 22000:2005 for food safety management in order to enable organizations can manage their processes and associated risks versus requirements of each internal and external stakeholder through only one MS instead of several individual MSs defined and implemented in an isolated way according to a specific MSS and hence to decrease the number of resources employed and to enhance the organization performance

Heritabilitet, fenotipske i genetske korelacije intenziteta porasta i mesnatosti svinja

The five year study included two genotypes of gilts of performance tested gilts, Swedish landrace and crosses F1 generation SLxLY. Of total number (n=3600), 1709 animals were genotype SL and 1891 animals were genotype SLxLY. Measuring of back fat thickness in the loin part (FT1), between 3rd and 4th lumbar vertebrae, 7cm laterally to the back line; back fat thickness (FT2) and depth of the musculus longissimus dorsi (MLD) between the 3rd and 4th rib from the rear, 7cm laterally to the back line. Assessment of meat yield was done using the ultrasonograph apparatus Piglog 105. In regard to meat yield indicators, medium heritability values were established for FT1 h2=0.461, and high values for FT2 h2=0.639, and for meat yield h2=0.633. Low heritability was established for depth of MLD (h2=0.105), life daily gain (h2=0.110) and age at the end of test (h2=0.103). Established phenotypic correlations between fat thickness FT1 and FT2 were strong (rp=0.638); between fat thickness and meat yield very strong (rp=-0.880 to -0.895), and between fat thickness and MLD very weak and negative (rg=-0.103 to -0.216). Genetic correlations were stronger than phenotypic, so between fat thickness FT1 and FT2 the correlation was complete/full (rg=0.930), also between fat thickness and meat yield (rg=-0.979 to -0.982), whereas the correlation between fat thickness and MLD was strong and negative (rg=-0.627 to -0.653). Heritability values for fat thickness and meat yield show that these traits have high level of heritability and are transfered to the progeny, whereas the level and strength of their dependance show that by decreasing the fat thickness positive influences is exhibited on meat yield, and that by increasing of depth of MLD also the meat yield is increased.Petogodišnjim istraživanjem su bila obuhvaćena dva genotipa nazimica švedski landras i melezi F1 generacije ŠLxVJ. Od ukupnog broja (n=3600) istraživanjem je obuhvaćeno 1709 grla genotipa ŠL i 1891 grla genotipa ŠLxVJ. Merenje debljine leđne slanine i dubine musculus longissimus dorsi (MLD) i procena mesnatosti je obavljeno ultrazvučnim aparatom Piglog 105. Za pokazatelje mesnatosti utvrđeni su srednji heritabiliteti za SL1 h2=0,461, a visoki za SL2 h2=0,639, i za mesnatost h2=0,633. Nizak heritabilitet utvrđen je za dubinu MLD-a (h2=0,105), životni dnevni prirast (h2=0,110) i za uzrast (h2=0,103). Utvrđene fenotipske korelacije između debljine slanine SL1 i SL2 su jake (rp=0,638); između debljine slanine i mesnatosti korelacije su vrlo jake (rp=-0,880 do -0,895), dok su između debljine slanine i MLD-a jako slabe i negativne (rg=-0,103 do -0,216). Genetske korelacije su jače od fenotipskih, tako da je između debljine slanine korelacija potpuna (rg=0,930), kao i između debljine slanine i mesnatosti (rg=-0,979 do -0,982), dok je korelacija jaka i negativna između debljine slanine i MLD-a (rg=-0,627 do -0,653). PR Projekat Ministarstva nauke Republike Srbije, br. TR 31081

Povezanost osobina veličine legla

Heredity and correlation of litter size traits were observed in 3693 litters, i.e. in 1622 daughters of two genotypes Swedish Landrace genotype - SL; and F1 ♀ SLxLW. The study included daughters (minimum ten daughters per sire) of 24 sires. Heritability estimates for the total number of piglets per litter in the first, the first two parities, and for all three parities was 0.174; 0.167 and 0.135. Heritability estimates for the number of piglets born alive were 0.181; 0.160 and 0.121, and for the weight of litter at birth 0.166; 0.174 and 0.150. On the other hand, very low heritability was determined for the number of weaned piglets, litter weight of piglets reared, individual weight of born and reared piglets, i.e. for the traits that are under greater influence of the environment (from 0.004 to 0.037). Phenotypic and genetic correlations ranged from weak to complete (rp = 0.021 to rp = 0.973 and rg=0.188 to rg=0.999, respectively). Analysis of the significance of correlations showed that the genetic correlations were statistically highly significant (P (lt) 0.01).Naslednost i međusobna povezanost osobina veličine legla praćene su za 3693 legla, odnosno kod 1622 kćeri dva genotipa švedski landras - ŠL; i F1 ♀ŠLxVJ. Ispitivanjem su obuhvaćene kćeri (minimalno deset kćeri po ocu) od 24 oca. Heritabiliteti za ukupan broj prasadi u leglu u prvom, prvom i drugom i za sva tri prašenja iznosio je 0,174; 0,167 i 0,135. Za broj živorođene prasadi vrednosi heritabiliteta su iznosile 0,181; 0,160 i 0,121, dok su za masu legla pri rođenju vrednosti 0,166; 0,174 i 0,150. Sa druge strane utvrđen je jako slab heritabilitet za broj odgajene prasadi, masu legla odgajene prasadi, individualnu masu rođene i odgajene prasadi odnosno za ispitivane osobine koje su pod većim uticajem okoline (od 0,004 do 0,037). Fenotipske i genetske korelacije kretale su se od slabih do potpunih (rp=0,021 to rp=0,973 odnosno rg=0,188 to rg=0,999). Testiranje značajnosti korelacija pokazalo je da su genetske bile statistički visoko značajne (P (lt) 0,01)

Povezanost osobina veličine legla

Heredity and correlation of litter size traits were observed in 3693 litters, i.e. in 1622 daughters of two genotypes Swedish Landrace genotype - SL; and F1 ♀ SLxLW. The study included daughters (minimum ten daughters per sire) of 24 sires. Heritability estimates for the total number of piglets per litter in the first, the first two parities, and for all three parities was 0.174; 0.167 and 0.135. Heritability estimates for the number of piglets born alive were 0.181; 0.160 and 0.121, and for the weight of litter at birth 0.166; 0.174 and 0.150. On the other hand, very low heritability was determined for the number of weaned piglets, litter weight of piglets reared, individual weight of born and reared piglets, i.e. for the traits that are under greater influence of the environment (from 0.004 to 0.037). Phenotypic and genetic correlations ranged from weak to complete (rp = 0.021 to rp = 0.973 and rg=0.188 to rg=0.999, respectively). Analysis of the significance of correlations showed that the genetic correlations were statistically highly significant (P (lt) 0.01).Naslednost i međusobna povezanost osobina veličine legla praćene su za 3693 legla, odnosno kod 1622 kćeri dva genotipa švedski landras - ŠL; i F1 ♀ŠLxVJ. Ispitivanjem su obuhvaćene kćeri (minimalno deset kćeri po ocu) od 24 oca. Heritabiliteti za ukupan broj prasadi u leglu u prvom, prvom i drugom i za sva tri prašenja iznosio je 0,174; 0,167 i 0,135. Za broj živorođene prasadi vrednosi heritabiliteta su iznosile 0,181; 0,160 i 0,121, dok su za masu legla pri rođenju vrednosti 0,166; 0,174 i 0,150. Sa druge strane utvrđen je jako slab heritabilitet za broj odgajene prasadi, masu legla odgajene prasadi, individualnu masu rođene i odgajene prasadi odnosno za ispitivane osobine koje su pod većim uticajem okoline (od 0,004 do 0,037). Fenotipske i genetske korelacije kretale su se od slabih do potpunih (rp=0,021 to rp=0,973 odnosno rg=0,188 to rg=0,999). Testiranje značajnosti korelacija pokazalo je da su genetske bile statistički visoko značajne (P (lt) 0,01)