76 research outputs found

    Book Reviews

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    Wellbeing and smart textiles: reflecting on collaborative practices and the design process

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    This paper reports on an inter-disciplinary, EPSRC funded research project, 'An Internet of Soft Things' (IoSofT) which seeks to bring soft surfaces, smart textiles and wearable technologies to join the Internet of Things debate. The project involves researchers from academic disciplines: design, computing and mental health in collaboration with a project partner, the Nottinghamshire network of the mental health charity, Mind. This paper will reflect upon the research project and specifically the approach the authors have taken to collaborative textile practice and how this has impacted upon the design process. This project was conducted through a number of practical workshop activities with Mind service user groups. The workshops focused upon the crafting of personalized textile objects with soft switches and various output and also recorded the clients' descriptions of their sense of ownership, awareness of their own and others' emotions and behaviours. The workshops included the researchers' reflections and observations to enable further understanding of how this community invests meaning in material things and modes of expressive output. The aim of the research project is to use textile craft practice and smart materials alongside therapeutic approaches to contribute to the development of a wellbeing and mental health toolkit to support future client work for Mind

    Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine

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    Orally administered phages to control zoonotic pathogens face important challenges, mainly related to the hostile conditions found in the gastrointestinal tract (GIT). These include temperature, salinity and primarily pH, which is exceptionally low in certain compartments. Phage survival under these conditions can be jeopardized and undermine treatment. Strategies like encapsulation have been attempted with relative success, but are typically complex and require several optimization steps. Here we report a simple and efficient alternative, consisting in the genetic engineering of phages to display lipids on their surfaces. Escherichia coli phage T7 was used as a model and the E. coli PhoE signal peptide was genetically fused to its major capsid protein (10A), enabling phospholipid attachment to the phage capsid. The presence of phospholipids on the mutant phages was confirmed by High Performance Thin Layer Chromatography, Dynamic Light Scattering and phospholipase assays. The stability of phages was analysed in simulated GIT conditions, demonstrating improved stability of the mutant phages with survival rates 102107 pfu.mL1 higher than wild-type phages. Our work demonstrates that phage engineering can be a good strategy to improve phage tolerance to GIT conditions, having promising application for oral administration in veterinary medicine.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and under the scope of the Project PTDC/BBB-BSS/6471/2014 (POCI-01-0145-FEDER-016678). Franklin L. Nobrega and Ana Rita Costa acknowledge FCT for grants SFRH/BD/86462/2012 and SFRH/BPD/94648/2013, respectively. Melvin F. Siliakus acknowledges funding from the Biobased Ecologically Balanced Sustainable Industrial Chemistry (BE-BASIC) foundation. Electron microscopy work was performed at the Wageningen Electron Microscopy Centre (WEMC) of Wageningen University
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