23 research outputs found
Microchannel heat transfer with slip flow and wall effects
Cataloged from PDF version of article.Analysis is presented for conjugate heat transfer in a parallel-plate microchannel. Axial conduction in the fluid and in the adjacent wall is included. The fluid is a constant property gas with a slip-flow velocity distribution. The microchannel is heated by a small region on the channel wall. The analytic solution is given in the form of integrals by the method of Green's functions. Quadrature is used to obtain numerical results for the temperature and heat transfer coefficient on the heated region for various Peclet number, Knudsen number, and wall materials. A region downstream of the heater is also explored. These results have application in the optimal design of small-scale heat transfer devices for biomedical applications, electronic cooling, and advanced fuel cells
Thermal performance of thermoactive continuous flight auger piles
Foundation piles are being increasingly equipped with heat exchangers to efficiently harvest shallow geothermal energy. For buildings in urban areas, continuous flight auger (CFA) piles are common owing to their speed, cost-efficiency and low noise levels. To construct a thermoactive CFA pile usually requires separate central installation of the heat exchanger. However, the energy performance of this type of pile has not been investigated systematically, with most studies focused on rotary piles where the heat exchanger is attached to the reinforcing cage. In this work, insights are provided about the main influences on the energy efficiency of thermoactive CFA piles, with a focus on the implications of using CFA construction techniques rather than rotary boring. An innovative three-dimensional numerical model, able to capture the different aspects of transient heat transfer, is employed together with analytical methods to evaluate the transient and steady-state behaviour of energy piles in a number of design situations. Attention is given to understanding the role of possible pipe-to-pipe interaction, which cannot be systematically investigated with standard methods. Finally, practical guidelines on the optimal choice of design parameters to maximise the energy efficiency of CFA piles, without altering the geotechnical arrangements, are provided
Amorphous formulations of indomethacin and griseofulvin prepared by electrospinning
Following an array of optimization
experiments, two series of electrospun
polyvinylpyrrolidone (PVP) fibers were prepared. One set of fibers
contained various loadings of indomethacin, known to form stable glasses,
and the other griseofulvin (a poor glass former). Drug loadings of
up to 33% w/w were achieved. Electron microscopy data showed the fibers
largely to comprise smooth and uniform cylinders, with evidence for
solvent droplets in some samples. In all cases, the drug was found
to exist in the amorphous physical state in the fibers on the basis
of X-ray diffraction and differential scanning calorimetry (DSC) measurements.
Modulated temperature DSC showed that the relationship between a formulationâs
glass transition temperature (<i>T</i><sub>g</sub>) and
the drug loading follows the GordonâTaylor equation, but not
the Fox equation. The results of GordonâTaylor analysis indicated
that the drug/polymer interactions were stronger with indomethacin.
The interactions between drug and polymer were explored in more detail
using molecular modeling simulations and again found to be stronger
with indomethacin; the presence of significant intermolecular forces
was further confirmed using IR spectroscopy. The amorphous form of
both drugs was found to be stable after storage of the fibers for
8 months in a desiccator (relative humidity <25%). Finally, the
functional performance of the fibers was studied; in all cases, the
drug-loaded fibers released their drug cargo very rapidly, offering
accelerated dissolution over the pure drug
Microchannel heat transfer with slip flow and wall effects
Analysis is presented for conjugate heat transfer in a parallel-plate microchannel. Axial conduction in the fluid and in the adjacent wall is included. The fluid is a constant property gas with a slip-flow velocity distribution. The microchannel is heated by a small region on the channel wall. The analytic solution is given in the form of integrals by the method of Green's functions. Quadrature is used to obtain numerical results for the temperature and heat transfer coefficient on the heated region for various Peclet number, Knudsen number, and wall materials. A region downstream of the heater is also explored. These results have application in the optimal design of small-scale heat transfer devices for biomedical applications, electronic cooling, and advanced fuel cells. © 2014 by the American Institute of Aeronautics and Astronautics, Inc
An RNA Interference (RNAi) Toolkit and Its Utility for Functional Genetic Analysis of <i>Leishmania</i> (<i>Viannia</i>)
RNA interference (RNAi) is a powerful tool whose efficacy against a broad range of targets enables functional genetic tests individually or systematically. However, the RNAi pathway has been lost in evolution by a variety of eukaryotes including most Leishmania sp. RNAi was retained in species of the Leishmania subgenus Viannia, and here we describe the development, optimization, and application of RNAi tools to the study of L. (Viannia) braziliensis (Lbr). We developed vectors facilitating generation of long-hairpin or âstem-loopâ (StL) RNAi knockdown constructs, using GatewayTM site-specific recombinase technology. A survey of applications of RNAi in L. braziliensis included genes interspersed within multigene tandem arrays such as quinonoid dihydropteridine reductase (QDPR), a potential target or modulator of antifolate sensitivity. Other tests include genes involved in cell differentiation and amastigote proliferation (A600), and essential genes of the intraflagellar transport (IFT) pathway. We tested a range of stem lengths targeting the L. braziliensis hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and reporter firefly luciferase (LUC) genes and found that the efficacy of RNAi increased with stem length, and fell off greatly below about 128 nt. We used the StL length dependency to establish a useful âhypomorphicâ approach not possible with other gene ablation strategies, with shorter IFT140 stems yielding viable cells with compromised flagellar morphology. We showed that co-selection for RNAi against adenine phosphoryl transferase (APRT1) using 4-aminopyrazolpyrimidine (APP) could increase the efficacy of RNAi against reporter constructs, a finding that may facilitate improvements in future work. Thus, for many genes, RNAi provides a useful tool for studying Leishmania gene function with some unique advantages
Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains
Staphylococcus aureus is a versatile pathogen capable of causing a broad range of diseases in many different hosts. S. aureus can adapt to its host through modification of its genome, e.g. by acquisition and exchange of mobile genetic elements that encode host-specific virulence factors. Recently the prophage ΊSaeq1 was discovered in S. aureus strains from six different clonal lineages almost exclusively isolated from equids. Within this phage we discovered a novel variant of Staphylococcal Complement Inhibitor (SCIN), a secreted protein that interferes with activation of the human complement system, an important line of host defense. We here show that this equine variant of SCIN, eqSCIN, is a potent blocker of equine complement system activation and subsequent phagocytosis of bacteria by phagocytes. Mechanistic studies indicate that eqSCIN blocks equine complement activation by specific inhibition of the C3 convertase enzyme (C3bBb). Whereas SCIN-A from human S. aureus isolates exclusively inhibits human complement, eqSCIN represents the first animal-adapted SCIN variant that functions in a broader range of hosts (horses, humans and pigs). Binding analyses suggest that the human-specific activity of SCIN-A is related to amino acid differences on both sides of the SCIN-C3b interface. These data suggest that modification of this phage-encoded complement inhibitor plays a role in the host adaptation of S. aureus and are important to understand how this pathogen transfers between different hosts
Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains
Staphylococcus aureus is a versatile pathogen capable of causing a broad range of diseases in many different hosts. S. aureus can adapt to its host through modification of its genome, e.g. by acquisition and exchange of mobile genetic elements that encode host-specific virulence factors. Recently the prophage ΊSaeq1 was discovered in S. aureus strains from six different clonal lineages almost exclusively isolated from equids. Within this phage we discovered a novel variant of Staphylococcal Complement Inhibitor (SCIN), a secreted protein that interferes with activation of the human complement system, an important line of host defense. We here show that this equine variant of SCIN, eqSCIN, is a potent blocker of equine complement system activation and subsequent phagocytosis of bacteria by phagocytes. Mechanistic studies indicate that eqSCIN blocks equine complement activation by specific inhibition of the C3 convertase enzyme (C3bBb). Whereas SCIN-A from human S. aureus isolates exclusively inhibits human complement, eqSCIN represents the first animal-adapted SCIN variant that functions in a broader range of hosts (horses, humans and pigs). Binding analyses suggest that the human-specific activity of SCIN-A is related to amino acid differences on both sides of the SCIN-C3b interface. These data suggest that modification of this phage-encoded complement inhibitor plays a role in the host adaptation of S. aureus and are important to understand how this pathogen transfers between different hosts