Quantifying the resuspension of nutrients and sediment by demersal trawling

Demersal fisheries trawling is widely acknowledged as one of the most intense forms of widespread benthic disturbance, resuspending extensive plumes of sediments and dissolved nutrients. However, difficulties associated with sampling within trawl plumes have hitherto limited our quantitative understanding of these widespread phenomena. This lack of knowledge hinders our ability to understand the broader consequences of demersal trawling and the development of new fishing gears to limit benthic disturbance. Here we present data from a series of novel in situ experiments using a specially designed trawl sled to quantitatively examine how trawl gear-induced drag and pressure influence the height and concentrations of resuspended sediments and nutrients within a trawl plume. Our data demonstrate that the composition of resuspended particles and sampled nutrients are both influenced by sampling height above the seafloor and the amount of drag exerted by the trawl gear (p < 0.001 in all cases), although the relative importance of these factors differed between the response variables examined. These differences likely reflect that sediment particles are more influenced by gravity than dissolved nutrients are. Our results demonstrate that trawl gear specification strongly influences the amount of dissolved and particulate material resuspended, suggesting that their design could be modified to reduce impacts on benthic ecosystems

ADAM10 and ADAM17 promote SARS‐CoV‐2 cell entry and spike protein‐mediated lung cell fusion

The severe‐acute‐respiratory‐syndrome‐coronavirus‐2 (SARS‐CoV‐2) is the causative agent of COVID‐19, but host cell factors contributing to COVID‐19 pathogenesis remain only partly understood. We identify the host metalloprotease ADAM17 as a facilitator of SARS‐CoV‐2 cell entry and the metalloprotease ADAM10 as a host factor required for lung cell syncytia formation, a hallmark of COVID‐19 pathology. ADAM10 and ADAM17, which are broadly expressed in the human lung, cleave the SARS‐CoV‐2 spike protein (S) in vitro, indicating that ADAM10 and ADAM17 contribute to the priming of S, an essential step for viral entry and cell fusion. ADAM protease‐targeted inhibitors severely impair lung cell infection by the SARS‐CoV‐2 variants of concern alpha, beta, delta, and omicron and also reduce SARS‐CoV‐2 infection of primary human lung cells in a TMPRSS2 protease‐independent manner. Our study establishes ADAM10 and ADAM17 as host cell factors for viral entry and syncytia formation and defines both proteases as potential targets for antiviral drug development

Sources, composition, and export of particulate organic matter across British estuaries

Estuaries receive and process a large amount of particulate organic carbon (POC) prior to its export into coastal waters. Studying the origin of this POC is key to understanding the fate of POC and the role of estuaries in the global carbon cycle. Here, we evaluated the concentrations of POC, as well as particulate organic nitrogen (PON), and used stable carbon and nitrogen isotopes to assess their sources across 13 contrasting British estuaries during five different sampling campaigns over 1 year. We found a high variability in POC and PON concentrations across the salinity gradient, reflecting inputs, and losses of organic material within the estuaries. Catchment land cover appeared to influence the contribution of POC to the total organic carbon flux from the estuary to coastal waters, with POC contributions >36% in estuaries draining catchments with a high percentage of urban/suburban land, and <11% in estuaries draining catchments with a high peatland cover. There was no seasonal pattern in the isotopic composition of POC and PON, suggesting similar sources for each estuary over time. Carbon isotopic ratios were depleted (−26.7 ± 0.42‰, average ± sd) at the lowest salinity waters, indicating mainly terrigenous POC (TPOC). Applying a two-source mixing model, we observed high variability in the contribution of TPOC at the highest salinity waters between estuaries, with a median value of 57%. Our results indicate a large transport of terrigenous organic carbon into coastal waters, where it may be buried, remineralized, or transported offshore

Data associated with the PhD thesis entitled: &quot;Investigating how benthic trawl gear design and location affect the resuspension of sediments and dissolved nutrients from the seafloor&quot;.

Data are for trawling experiments carried out in the Dornoch Firth area, Scotland for the years 2015, 2016 and 2017. Data were used in the thesis: &quot;Investigating how benthic trawl gear design and location affect the resuspension of sediments and dissolved nutrients from the seafloor&quot;. Data in the 2015 file includes trawl component type (dimensions of the roller) and associated drag and pressure, and the resulting nutrient and Particle Size Distribution (PSD) data at different heights of the seafloor. Samples were collected at the Hollows and Dornoch Bay sites in the Dornoch Firth area on the 08/05/2015. Data in the 2016 file includes trawl component type (dimensions of the roller) and associated drag with consistent pressure (no added weights), and the resulting nutrient and Particle Size Distribution (PSD) data at different heights of the seafloor. Samples were collected at 6 sites: Banff, Hollows, Dornoch offshore, Lossie, Lossie prawn fishery and Dornoch offshore, between the 26/08/2016 to 04/10/2016. Data in the 2017 file includes a single trawl component type (400 Block, 600 long) and associated drag with added weights to simulate increasing pressure on the seafloor (tied up, 0kg, 60kg, 120kg). The resulting nutrient and Particle Size Distribution (PSD) data at different heights of the seafloor are reported. Samples were collected at 6 different sites: 2 at Banff-Buckie, 3 in the area of Lossie-Burghead and 1 at Dornoch. Trawls were conducted between the 01/08/2017 and 08/08/2017. This work was supported by a NERC CASE PhD studentship (NE/N007999/1). </span

Contrasting Estuarine Processing of Dissolved Organic Matter Derived From Natural and Human-Impacted Landscapes

The flux of terrigenous organic carbon through estuaries is an important and changing, yet poorly understood, component of the global carbon cycle. Using dissolved organic carbon (DOC) and fluorescence data from 13 British estuaries draining catchments with highly variable land uses, we show that land use strongly influences the fate of DOC across the land ocean transition via its influence on the composition and lability of the constituent dissolved organic matter (DOM). In estuaries draining peatland-dominated catchments, DOC was highly correlated with biologically refractory “humic-like” terrigenous material which tended to be conservatively transported along the salinity gradient. In contrast, there was a weaker correlation between DOC and DOM components within estuaries draining catchments with a high degree of human impact, that is, relatively larger percentage of arable and (sub)urban land uses. These arable and (sub)urban estuaries contain a high fraction of bioavailable “protein-like” material that behaved nonconservatively, with both DOC removals and additions occurring. In general, estuaries draining catchments with a high percentage of peatland (≥18%) have higher area-specific estuarine exports of DOC (>13 g C m−2 yr−1) compared to those estuaries draining catchments with a high percentage (≥46%) of arable and (sub)urban land uses (<2.1 g C m−2 yr−1). Our data indicate that these arable and (sub)urban estuaries tend to export, on average, ∼50% more DOC to coastal areas than they receive from rivers due to net anthropogenic derived organic matter inputs within the estuary