Impact of frequency of feedline soaking combined with evaporative air cooling in a humid environment

Heat stress in hot and humid environments reduces milk production, decreases reproduction, and increases health-related problems. The summertime environment in north-central Florida is especially difficult because the combination of high relative humidity and high temperature results in a temperaturehumidity index (THI) above the critical value of 72 for significant portions of the day. Previous work at Kansas State University had shown that the combination of soaking and evaporative air cooling could effectively cool heat-stressed cattle. Effectiveness of this feedline soaking, either in the afternoon and at night, or only at night, in combination with evaporative cooling was evaluated on a commercial dairy located in north-central Florida. A high-pressure fogging system and feedline soakers were installed in a typical 4-row freestall barn equipped with tunnel ventilation creating a north to south airflow of 6 to 8 mph at the cow level. Eight lactating Holstein cows in each of two, 292-stall pens were selected and fitted with vaginal temperature probes. Data on vaginal temperature and respiration rate were used to evaluate two cooling treatments. Barn temperature averaged 74.8 ± 5.4ºF, relative humidity was 84.6 ± 15.4 %, and THI was 74.7 ± 5.3 during the study. The evaporative cooling system reduced average barn temperature by 0.9ºF and reduced afternoon temperatures by a maximum of 9.2ºF. Average respiration rates were less (58.5 vs. 66.9 breaths/min) in the afternoon and night soaking treatment, compared with the respiration rate of cattle in the night soaking treatment. Differences were greatest at the 10:00 p.m. observation (55.0 vs. 73.3 breaths/min). Average vaginal temperature was also less (102.0 vs. 102.6°F) in the afternoon and night soaking treatment. Our results indicate that the combination of cooling the air via a highpressure fogging system and feedline soaking reduced heat stress experienced by dairy cattle. Using feedline soaking during the afternoon and night was more effective than soaking only at night.; Dairy Day, 2005, Kansas State University, Manhattan, KS, 2005; Dairy Research, 2005 is known as Dairy Day, 200

Genome-wide significant schizophrenia risk variation on chromosome 10q24 is associated with altered cis-regulation of BORCS7, AS3MT, and NT5C2 in the human brain

Chromosome 10q24.32-q24.33 is one of the most robustly supported risk loci to emerge from genome-wide association studies (GWAS) of schizophrenia. However, extensive linkage disequilibrium makes it difficult to distinguish the actual susceptibility gene(s) at the locus, limiting its value for improving biological understanding of the condition. In the absence of coding changes that can account for the association, risk is likely conferred by altered regulation of one or more genes in the region. We, therefore, used highly sensitive measures of allele-specific expression to assess cis-regulatory effects associated with the two best-supported schizophrenia risk variants (SNP rs11191419 and indel ch10_104957618_I/rs202213518) on the primary positional candidates BORCS7, AS3MT, CNNM2, and NT5C2 in the human brain. Heterozygosity at rs11191419 was associated with increased allelic expression of BORCS7 and AS3MT in the fetal and adult brain, and with reduced allelic expression of NT5C2 in the adult brain. Heterozygosity at ch10_104957618_I was associated with reduced allelic expression of NT5C2 in both the fetal and adult brain. Comparisons between cDNA ratios in heterozygotes and homozygotes for the risk alleles indicated that cis-effects on NT5C2 expression in the adult dorsolateral prefrontal cortex could be largely accounted for by genotype at these two risk variants. While not excluding effects on other genes in the region, this study implicates altered neural expression of BORCS7, AS3MT, and NT5C2 in susceptibility to schizophrenia arising from genetic variation at the chromosome 10q24 locus

What America means to sixth grade children.

Thesis (Ed.M.)--Boston Universit

A quasi classical approach to fully differential ionization cross sections

A classical approximation to time dependent quantum mechanical scattering in the M\o{}ller formalism is presented. Numerically, our approach is similar to a standard Classical-Trajectory-Monte-Carlo calculation. Conceptually, however, our formulation allows one to release the restriction to stationary initial distributions. This is achieved by a classical forward-backward propagation technique. As a first application and for comparison with experiment we present fully differential cross sections for electron impact ionization of atomic hydrogen in the Erhardt geometry.Comment: 6 pages, 2 figure

The Reaction Process A+A->O in Sinai Disorder

The single-species reaction-diffusion process $A+A\to O$ is examined in the presence of an uncorrelated, quenched random velocity field. Utilising a field-theoretic approach, we find that in two dimensions and below the density decay is altered from the case of purely diffusing reactants. In two-dimensions the density amplitude is reduced in the presence of weak disorder, yielding the interesting result that Sinai disorder can cause reactions to occur at an {\it increased} rate. This is in contrast to the case of long-range correlated disorder, where it was shown that the reaction becomes sub-diffusion limited. However, when written in terms of the microscopic diffusion constant it is seen that increasing the disorder has the effect of reducing the rate of the reaction. Below two dimensions, the effect of Sinai disorder is much more severe and the reaction is shown to become sub-diffusion limited. Although there is no universal amplitude for the time-dependence of the density, it is universal when expressed in terms of the disorder-averaged diffusion length. The appropriate amplitude is calculated to one-loop order.Comment: 12 pages, 2 figure

Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea.

The HerA ATPase cooperates with the NurA nuclease and the Mre11-Rad50 complex for the repair of double-strand DNA breaks in thermophilic archaea. Here we extend our structural knowledge of this minimal end-resection apparatus by presenting the first crystal structure of hexameric HerA. The full-length structure visualizes at atomic resolution the N-terminal HerA-ATP synthase domain and a conserved C-terminal extension, which acts as a physical brace between adjacent protomers. The brace also interacts in trans with nucleotide-binding residues of the neighbouring subunit. Our observations support a model in which the coaxial interaction of the HerA ring with the toroidal NurA dimer generates a continuous channel traversing the complex. HerA-driven translocation would propel the DNA towards the narrow annulus of NurA, leading to duplex melting and nucleolytic digestion. This system differs substantially from the bacterial end-resection paradigms. Our findings suggest a novel mode of DNA-end processing by this integrated archaeal helicase-nuclease machine.The SAXS data collection was supported by funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement N°283570). Research in the N.P.R. laboratory is funded by the Medical Research Council [Career Development Award G0701443]. Research in the L.P. laboratory is funded by a Wellcome Trust Senior Fellowship Award in Basic Biomedical Sciences [grant number 08279/Z/07/Z]. Work in the L.P. and N.P.R. laboratories is also supported by an Isaac Newton Trust Research Grant, and S.M.B. is supported by a BBSRC Doctoral Training Grant.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/ncomms650

Anything You Can Do, You Can Do Better: Neural Substrates of Incentive-Based Performance Enhancement

Performance-based pay schemes in many organizations share the fundamental assumption that the performance level for a given task will increase as a function of the amount of incentive provided. Consistent with this notion, psychological studies have demonstrated that expectations of reward can improve performance on a plethora of different cognitive and physical tasks, ranging from problem solving to the voluntary regulation of heart rate. However, much less is understood about the neural mechanisms of incentivized performance enhancement. In particular, it is still an open question how brain areas that encode expectations about reward are able to translate incentives into improved performance across fundamentally different cognitive and physical task requirements