Entwicklung und Bewertung von direkten Bekämpfungsstrategien gegen das Auftreten der Möhrenschwärze (Alternaria dauci) beim Anbau von Möhren für die industrielle Verarbeitung

Im Rahmen des BLE-Forschungsprojektes „Entwicklung und Bewertung von direkten Bekämpfungsstrategien gegen das Auftreten der Möhrenschwärze (Alternaria dauci) beim Anbau von Möhren für die industrielle Verarbeitung“ (03OE488) wurde in den Jahren 2004-2006 Feldversuche zur Optimierung des ökologischen Möhrenanbaus unter norddeutschen Bedingungen durchgeführt. Sortenvergleichsanbau In den drei Jahren wurden auf den zwei Versuchsstandorten jeweils 10 bis 12 Möhrensorten auf ihre Eignung für den ökologischen Anbau geprüft. Wesentliche Kriterien waren hierbei die Widerstandsfähigkeit gegenüber Alternaria, wertgebende Inhaltstoffe und die Ertragshöhe. Es konnten deutliche Unterschiede in der Alternariaanfälligkeit der geprüften Sorten beobachtet werden. Dabei zeigte sich, dass diese Sortenunterschiede sowohl an beiden Versuchsstandorten als auch über die geprüften Jahre auftraten. Das Ergebnis der Sortenprüfung belegte, dass die bisher überwiegend im ökologischen Anbau eingesetzten Sorten sich als deutlich weniger geeignet erwiesen, als andere geprüfte Sorten. Die hohe Alternariaanfälligkeit der bisher verwendeten Standardsorten führte bei entsprechenden Infektionsbedingungen zu einer starken Laubschädigung mit deutlichem Einfluss auf den Nitratgehalt und die Vermarktungsfähigkeit des Ernteproduktes. Anbauversuch zur Wirksamkeit der Heißwasserbehandlung (HWB) des Saatgutes auf den Laubbefall mit Alternaria In diesem Teilversuch wurde anhand von alternariaanfälligen Möhrensorten überprüft, ob ein Besatz des Möhrensaatgutes mit dem pilzlichen Erreger Alternaria dauci einen Einfluss auf den Zeitpunkt und die Stärke eines Alternariabefalles im Möhrenbestand hat. Dazu wurde unbehandeltes gering belastetes Saatgut, sowie stark belastetes Saatgut mit heißwasserbehandeltem Saatgut verglichen. Im Versuchsjahr 2004 trat an keiner Versuchsvariante ein Alternaria Befall auf. Dagegen konnte in den Versuchsjahren 2005 und 2006 nachgewiesen werden, dass ein stark erhöhter Ausgangsbesatz des Saatgutes mit Alternaria dauci bei entsprechenden Infektionsbedingungen auch zu einem stärkeren Auftreten der Laubkrankheit im Bestand führte. Die Frage des tolerierbaren Grenzwertes für den Besatz des Saatgutes mit Alternaria dauci konnte in diesem Forschungsvorhaben nicht geklärt werden. Besonders bei Alternaria anfälligen Sorten sollte aber auf eine möglichst geringe Ausgangsbelastung des Saatgutes geachtet werden. Aber auch die Nachteile einer verringerte Keimfähigkeit sowie einer verminderten Haltbarkeit des Saatgutes durch eine HWB müssen beim Saatguteinkauf berücksichtigt werden. Feststellen der Wirksamkeit verschiedener Pflanzenschutz und –stärkungsmittel gegen Laubkrankheiten (speziell Alternaria) In diesem Teilversuch wurde die direkte Wirkung von Pflanzenschutzmitteln und Pflanzenstärkungsmitteln auf den Befall mit Alternaria dauci überprüft. Ergänzend wurde der Einsatz von Haftmitteln und Spurenelementdüngern geprüft. In allen drei Versuchsjahren konnte durch den Einsatz von Kupferpräparaten ein deutlicher Effekt auf den Alternariabefall der Blattfläche nachgewiesen werden. Dies betrifft sowohl die Cuprozin-Varianten auf Basis von Kupferhydroxid als auch die Cueva-Variante auf Basis von Kupferoktanoat. Es ist zu beachten, dass der Wirkstoff Kupferoktanoat bisher nicht im Anhang der EU-Öko-Verordnung als erlaubtes Pflanzenschutzmittel aufgeführt ist

Cylindrocladiella parva as a causal agent of dieback on Euonymus fortunei

Im August 2011 traten an Euonymus fortunei Absterbe­erscheinungen unbekannter Ursache auf. Aus befallenen Trieben wurde ein Cylindrocladium-ähnlicher Pilz isoliert. Anhand seiner morphologischen Merkmale wurde der Pilz als Cylindrocladiella parva (P.J. Anderson) Boesewinkel identifiziert. Eine anschließende Sequenzanalyse der ITS-Region sowie des β-Tubulin-Gens bestätigte das Ergebnis. Die Pathogenität des Pilzes an E. fortunei 'Emerald’n Gold' wurde in mehreren Versuchsansätzen geprüft. Nach Inokulation an den Nodien nach leichter Verletzung entwickelten sich bei hoher Luftfeuchte innerhalb von sieben Wochen an zweijährigen Pflanzen deut­liche Krankheitssymptome. Cylindrocladiella parva ließ sich aus befallenen Trieben reisolieren. Die Pathogenität dieses Erregers an E. fortunei ist damit nachgewiesen. Es ist das erste bekannt gewordene Auftreten von Cylindro­cladiella parva an E. fortunei in Deutschland. DOI: 10.5073/JfK.2014.01.01, https://doi.org/10.5073/JfK.2014.01.01In August 2011 a shoot dieback of unknown cause was observed on Euonymus fortunei. From the affected shoots a fungus similar to Cylindrocladium was isolated. According to its morphological characteristics this fungus could be identified as Cylindrocladiella parva (P.J. Anderson) Boesewinkel. The subsequent sequence analysis of the ITS regions as well as the β-tubulin-gene confirmed this result. Pathogenicity of this fungus was tested in several trials on E. fortunei 'Emerald’n Gold'. After inoculation of wounded nodes with the fungus and incubation with high humidity two year old plants developed disease symptoms within seven weeks. Cylindrocladiella parva could be reisolated from infected shoots. This is the first occurrence of Cylindrocladiella parva on E. fortunei in Germany according to our knowledge. DOI: 10.5073/JfK.2014.01.01, https://doi.org/10.5073/JfK.2014.01.0

Inflammation, fibrosis and skeletal muscle regeneration in LGMDR9 are orchestrated by macrophages

Aims: Variable degrees of inflammation, necrosis, regeneration and fibrofatty replacement are part of the pathological spectrum of the dystrophic process in alpha dystroglycanopathy LGMDR9 (FKRP-related, OMIM #607155), one of the most prevailing types of LGMDs worldwide. Inflammatory processes and their complex interplay with vascular, myogenic and mesenchymal cells may have a major impact on disease development. The purpose of our study is to describe the specific immune morphological features in muscle tissue of patients with LGMDR9 to enable a better understanding of the phenotype of muscle damage leading to disease progression. Methods: We have analysed skeletal muscle biopsies of 17 patients genetically confirmed as having LGMDR9 by histopathological and molecular techniques. Results: We identified CD206+ MHC class II+ and STAT6+ immune-repressed macrophages dominating the endomysial infiltrate in areas of myofibre regeneration and fibrosis. Additionally, PDGFRβ+ pericytes were located around MHC class II+ activated capillaries residing in close proximity to areas of fibrosis and regenerating fibres. Expression of VEGF was found on many regenerating neonatal myosin+ fibres, myofibres and CD206+ macrophages also co-expressed VEGF. Conclusion: Our results show characteristic immune inflammatory features in LGMDR9 and more specifically shed light on the predominant role of macrophages and their function in vascular organisation, fibrosis and myogenesis. Understanding disease-specific immune phenomena potentially inform about possibilities for anti-fibrotic and anti-inflammatory therapeutic strategies, which may complement Ribitol replacement and gene therapies for LGMDR9 that may be available in the future

Дослідження метрик маршрутизаторів глобальних мереж передачі даних

Об'єкт дослідження: метрики маршрутизаторів глобальних мереж. Мета дипломної роботи: розробка математичних моделей і методів маршрутизації глобальних мереж передачі даних в системі зі змінними параметрами на основі розробки узагальненої метрики маршрутизації при виборі оптимального маршруту. У вступі приведена загальна характеристика роботи, обгрунтована актуальність теми дослідження. У першому розділі розглянуто принципи маршрутизації, механізми маршрутизації і їх метрики, протоколи RIP, EIGRP, OSPF. У другому розділі проведено системний аналіз поняття маршрутизації за вибором шляхів передачі даних в глобальних мережах, проведено аналіз теоретичних основ маршрутизації на основі графових методів опису мереж, сформульовані завдання по найкоротшому шляху, що дозволяють вирішувати завдання маршрутизації. У третьому розділі дипломної роботи розрахована економічна ефективність, проаналізовані показники і зроблено висновок про доцільність використання даного проекту

A scalable system to measure contrail formation on a per-flight basis

Persistent contrails make up a large fraction of aviation's contribution to global warming. We describe a scalable, automated detection and matching (ADM) system to determine from satellite data whether a flight has made a persistent contrail. The ADM system compares flight segments to contrails detected by a computer vision algorithm running on images from the GOES-16 Advanced Baseline Imager. We develop a 'flight matching' algorithm and use it to label each flight segment as a 'match' or 'non-match'. We perform this analysis on 1.6 million flight segments. The result is an analysis of which flights make persistent contrails several orders of magnitude larger than any previous work. We assess the agreement between our labels and available prediction models based on weather forecasts. Shifting air traffic to avoid regions of contrail formation has been proposed as a possible mitigation with the potential for very low cost/ton-CO2e. Our findings suggest that imperfections in these prediction models increase this cost/ton by about an order of magnitude. Contrail avoidance is a cost-effective climate change mitigation even with this factor taken into account, but our results quantify the need for more accurate contrail prediction methods and establish a benchmark for future development.Comment: 25 pages, 6 figure

A Pair of Dopamine Neurons Target the D1-Like Dopamine Receptor DopR in the Central Complex to Promote Ethanol-Stimulated Locomotion in Drosophila

Dopamine is a mediator of the stimulant properties of drugs of abuse, including ethanol, in mammals and in the fruit fly Drosophila. The neural substrates for the stimulant actions of ethanol in flies are not known. We show that a subset of dopamine neurons and their targets, through the action of the D1-like dopamine receptor DopR, promote locomotor activation in response to acute ethanol exposure. A bilateral pair of dopaminergic neurons in the fly brain mediates the enhanced locomotor activity induced by ethanol exposure, and promotes locomotion when directly activated. These neurons project to the central complex ellipsoid body, a structure implicated in regulating motor behaviors. Ellipsoid body neurons are required for ethanol-induced locomotor activity and they express DopR. Elimination of DopR blunts the locomotor activating effects of ethanol, and this behavior can be restored by selective expression of DopR in the ellipsoid body. These data tie the activity of defined dopamine neurons to D1-like DopR-expressing neurons to form a neural circuit that governs acute responding to ethanol

Research inventory of child health: A report on roadmaps for the future of child health research in Europe

RICHE was the response to a call under HEALTH-2009-3.3-5, with the title of 'European child health research platform'. The call text asked us to “address the diversity and fragmentation in child health research in Europe in an inclusive multidisciplinary way, identifying existing research programmes in Member States, recent advances and identification of gaps to explore road maps for the future of child health research in Europe”. Project structure A consortium, with a final total of 23 partners, and two associate (i.e. unfunded) partners, responded to this call. We designed a project with a linear structure, where the main focus of activity moved from work on the Inventory, and Indicators and Measurement, in Year 1, to work on Gaps in Year 2, finishing with the preparation of the Roadmap in year 3. The final 6 months (Year 4) were largely dissemination. The Platform, which is instantiated in our website, supported all of the other parts,, and was a focus for communication and dissemination throughout the project. Each workpackage focused on a specific area of work, but each fed into its successors, and all leaders and partners worked closely together. Each group produced a number of technical reports and other outputs. The final output was a Roadmap for future investment in European child health research. This has been widely disseminated, and has fed at Commission level and National level into the Horizon 2020 call preparation process. RICHE Roadmap The RICHE Roadmap is based upon a sound, scientific evidence base, which we had gathered as part of our earlier work. The project prepared an inventory of child health research and of measurements and indicators of child health in Europe (WP1 and WP2) . This was collated using a web platform – which can be found at www.childhealthresearch.eu. In addition to this exercise, a formal study of the gaps in child health research was undertaken by carrying out surveys and interviews of researchers and research users across Europe (WP3). This allowed our initial views on the research gaps to be refined and corrected by an iterative process, involving, both project partners, and the wider scientific community, so seeking grounding and validation for this key phase. These results formed the basis for the RICHE Roadmap. The Roadmap is based on a life-course perspective. It covers the important phases of a child’s development, including maternal health, and pregnancy, through to adolescence and the protective and risk factors, and health services encountered throughout childhood and adolescence as he or she moves towards adulthood. RICHE looked upstream to identify where more work needed to be done to prevent avoidable physical and mental ill-health, disability and death in the population of European children aged 0-18 years. This shows how the many influences and outcomes of children’s health are interrelated; a pattern reflected in the Roadmap. The work necessarily involved a series of value judgements, especially on setting priorities, because there are no objective and unconditionally valid answers to the question “Is there enough research on this topic?”, nor to the question “Is this a topic of significance?”. Nevertheless, the RICHE Roadmap uses an inclusive and transparent process to explain the recommendations it made, and the subjects it chose, making our values, and the reasons for judgements as explicit as possible. The report is organised into broad subject areas, that reflect the key ‘gaps’ in knowledge about children and young people, or about particular aspects of their lifestyle and health. These key areas, and selected findings within each area, are briefly summarised here. Life Course and Lifestyle This section focuses on children as they age, and recognises the importance of continuing to research how factors before conception, during gestation and in the very early years of life can affect present and future health. The challenges that children face as they grow up are also highlighted – these can be created because of policy decisions that fail to take account of children and young people’s lives or because of the pervasive influences of individual circumstances that act ask protective or risk factors for children’s actions. The concept of resilience in childhood is also highlighted, and how research needs to focus on this important and powerful means of improving children’s lives. A key issue, throughout the life course, is mental health and well-being. Fostering well-being in children from birth, and throughout childhood will provide numerous individual and societal benefits. It deserves a greater research focus. Socio-economic and Cultural Factors The socio-economic and cultural environment in which a child is born and grows up has a potent effect on a child’s health and well-being. Inequity and inequalities in health, between and within nations depending on socio-economic circumstances, are known to affect health outcomes. Those in the poorest areas have worse health, and shorter lives than those in the wealthiest areas. Other groups are at risk of marginalisation from health services and from opportunities that can maximise their health. These include migrant children where the question is how best to support their integration into their new societies and communities, while retaining their individual identity; children in the state care system have poor health and social outcomes, so improving these, by focused research is important for the future health of these children; children from minority population groups, in particular those who travel across nations, such as the Roma, need to have focused attention, to ensure that their health outcomes begin to match those of the general European child population. Social and Community Networks The main influences on children and young people are their immediate family and community networks. This extends from the influence of the family as a warm and nurturing environment in which to grow up – and conversely a place of the most profound danger and threat if such a family environment is toxic; to the wider influence of school, and finally the broader community. Becoming engaged and involved in community life is beneficial for the entire population, not just for the children and young people directly involved. It is an aspect of children and young people’s experience that is important for well-being and social inclusion. Environment The term 'environment' covers several different concepts, and the RICHE Roadmap describes the physical, virtual and also the perceived environment – all of which interlink in children’s lives, and have a profound effect on their health and development. These include the physical environment, the virtual (digital) environment, physical safety, including injury prevention, and protection form crime, anti-social behaviour and violence (both as perpetrators and victims). Complex Health Issues The majority of children in Europe are healthy, and ill-health is not a characteristic of this population as it is in, for example, an ageing population. However, there are certain health issues that affect children, and as such can blight an entire lifetime. Our Roadmap does not cover clinical issues, but takes a population perspective. There are certain disorders that have a population-wide effect and are prevalent enough in the child population to warrant particular attention from a public health viewpoint. Four specific areas of concern were identified – overweight and obesity, mental health, sexual and reproductive health, and neuro-developmental disorders. Health Services The main research needs of the health services focuses on the prevention of poor health. Comparing health services across Europe and evaluating the means of conveying health promotion messages are important directions for health research to investigate. Indicators need developing which reflect the effect of preventive actions, particularly among younger children. Vulnerable populations, such as those in deprived communities, need to have health prevention services particularly targeted. There is little systematic evaluation of such interventions, which compromises the development of new interventions and their implementation. Those who do not access services and those who need particular attention can be identified. Public Health Infrastructure Health surveillance is essential so that health needs can be identified and addressed effectively for the benefit of the child population. Yet, many existing sources of data are neither analysed, nor made available in a child-centric way. Children need to be made more visible in the data so that they can have more effective health promotion and health care on a population level. Specific examples include work on autism and morbidity due to injury. Europe also needs to establish proper measures and indicators of children’s health and children’s lives. We cannot act properly without first identifying and measuring the problem. Electronic health records are an emerging technology that has great potential, both for research, and for improved access to care. They need to be developed and investigated further to encourage their use across the European Union. Improving Research Capacity It is necessary to ensure that there are enough resources, both to do research, and to make use of the research findings. To sustain research activity, specialist training for junior child health researchers is needed, as are sufficient resources to maintain a critical mass of researchers and provide attractive career paths for them. Children and young people as subjects of research need to continue to be safeguarded by a consistently ethical framework, and information collected about children needs to be accurate, comprehensive and used intelligently so that interventions and services can be correctly directed. Using the roadmap The roadmap is a complex document, addressed to a number of different stakeholders. One key group are those who make decision bout research finding priorities. We have disseminated the roadmap widely at EU level, to reach into the process of priority setting for Horizon 2020. This has been done thought National Contact Points in each partner country, through relevant NGO's, and by sending copes to and meeting with relevant parts of the European Commission. Readers using the roadmap will most likely use it in two ways, first to make a general case for investment in Child Health Research, and secondly to target that investment, by considering the questions we have identified, and reviewing our justifications for these choices. We do not expect our work to determine future investments in child health, but we are confident that using our work would lead to better decisions overall. Conclusions Our core value is to put children first in our work. We take the rights of the child seriously, and we are conscious that many children do not have the opportunity to exercise the right to health and healthcare that European children they ought to have. The topics in this Roadmap are pragmatic in that they are researchable (within the grasp of presently available research methods and resources) and that are likely to have a significant effect on the lives of European children. This will go a considerable distance in improving the health and well-being of European children who may not have benefited from Europe’s good fortune up until this point. At the very least, the RICHE Roadmap aims to begin a serious conversation across Europe about the need for research to focus on children and how this will ultimately benefit all members of the European population. There is a need for children to become substantially more visible in European society. At present many children’s lives are invisible to health surveillance and to research. Sometimes they are submerged with their families, as in the case of Roma or for children of illegal and undocumented immigrant families. Even in well-documented societies, children’s circumstances are invisible as data are collected from the perspective of economically active adults, or households. Therefore, an overarching recommendation in this road map is the establishment of a European Child Health Observatory with a simple remit to make European Children, and their lives, health and attainment of rights more visible. We also recommend continuing and extending the discussion to the edge of existing child health boundaries, to address topics such as the effects on children’s health of urban design and architecture, fiscal policy (which can affect many health issues), welfare, or health effects of immigration policy. The RICHE Roadmap hopes to point the way in which children can be fully recognised and respected as forming a valuable population and whose health and well-being contributes to the health of our present and future European society

The (in)stability of money demand in the euro area: lessons from a cross-country analysis

The instability of standard money demand functions has undermined the role of monetary aggregates for monetary policy analysis in the euro area. This paper uses country-specific monetary aggregates to shed more light on the economics behind the instability of euro area money demand. Our results obtained from panel estimation indicate that the observed instability of standard money demand functions could be explained by omitted variables like e.g. technological progress that are important for money demand but constant across member countries