Vector competence of Aedes aegypti, Culex tarsalis, and Culex quinquefasciatus from California for Zika virus.

Zika virus (ZIKV) has emerged since 2013 as a significant global human health threat following outbreaks in the Pacific Islands and rapid spread throughout South and Central America. Severe congenital and neurological sequelae have been linked to ZIKV infections. Assessing the ability of common mosquito species to transmit ZIKV and characterizing variation in mosquito transmission of different ZIKV strains is important for estimating regional outbreak potential and for prioritizing local mosquito control strategies for Aedes and Culex species. In this study, we evaluated the laboratory vector competence of Aedes aegypti, Culex quinquefasciatus, and Culex tarsalis that originated in areas of California where ZIKV cases in travelers since 2015 were frequent. We compared infection, dissemination, and transmission rates by measuring ZIKV RNA levels in cohorts of mosquitoes that ingested blood meals from type I interferon-deficient mice infected with either a Puerto Rican ZIKV strain from 2015 (PR15), a Brazilian ZIKV strain from 2015 (BR15), or an ancestral Asian-lineage Malaysian ZIKV strain from 1966 (MA66). With PR15, Cx. quinquefasciatus was refractory to infection (0%, N = 42) and Cx. tarsalis was infected at 4% (N = 46). No ZIKV RNA was detected in saliva from either Culex species 14 or 21 days post feeding (dpf). In contrast, Ae. aegypti developed infection rates of 85% (PR15; N = 46), 90% (BR15; N = 20), and 81% (MA66; N = 85) 14 or 15 dpf. Although MA66-infected Ae. aegypti showed higher levels of ZIKV RNA in mosquito bodies and legs, transmission rates were not significantly different across virus strains (P = 0.13, Fisher's exact test). To confirm infectivity and measure the transmitted ZIKV dose, we enumerated infectious ZIKV in Ae. aegypti saliva using Vero cell plaque assays. The expectorated plaque forming units PFU varied by viral strain: MA66-infected expectorated 13±4 PFU (mean±SE, N = 13) compared to 29±6 PFU for PR15-infected (N = 13) and 35±8 PFU for BR15-infected (N = 6; ANOVA, df = 2, F = 3.8, P = 0.035). These laboratory vector competence results support an emerging consensus that Cx. tarsalis and Cx. quinquefasciatus are not vectors of ZIKV. These results also indicate that Ae. aegypti from California are efficient laboratory vectors of ancestral and contemporary Asian lineage ZIKV

Generational Shift and Drug Abuse in Older Americans

Since the problem of substance abuse by older adults began to receive serious professional attention, the focus has been almost exclusively on alcohol abuse. This reality is changing as the “baby boomers” age. We examined data from the National Household Survey on Drug Abuse (1985) and the National Survey on Drug Use and Health (2006) comparing nationwide prevalence of both legal and illicit drugs. Use of drugs by category varied over the study period; some showed little change (e.g., alcohol) while others showed marked increase (e.g., marijuana, cocaine). We found that the population of older Americans in 2006 contained greater proportions of persons who had used or continued to use illicit drugs than did older Americans in 1985. Gerontologists and geriatric services will increasingly encounter older Americans who use or abuse illicit drugs

Automatic Reconstruction of Fault Networks from Seismicity Catalogs: 3D Optimal Anisotropic Dynamic Clustering

We propose a new pattern recognition method that is able to reconstruct the 3D structure of the active part of a fault network using the spatial location of earthquakes. The method is a generalization of the so-called dynamic clustering method, that originally partitions a set of datapoints into clusters, using a global minimization criterion over the spatial inertia of those clusters. The new method improves on it by taking into account the full spatial inertia tensor of each cluster, in order to partition the dataset into fault-like, anisotropic clusters. Given a catalog of seismic events, the output is the optimal set of plane segments that fits the spatial structure of the data. Each plane segment is fully characterized by its location, size and orientation. The main tunable parameter is the accuracy of the earthquake localizations, which fixes the resolution, i.e. the residual variance of the fit. The resolution determines the number of fault segments needed to describe the earthquake catalog, the better the resolution, the finer the structure of the reconstructed fault segments. The algorithm reconstructs successfully the fault segments of synthetic earthquake catalogs. Applied to the real catalog constituted of a subset of the aftershocks sequence of the 28th June 1992 Landers earthquake in Southern California, the reconstructed plane segments fully agree with faults already known on geological maps, or with blind faults that appear quite obvious on longer-term catalogs. Future improvements of the method are discussed, as well as its potential use in the multi-scale study of the inner structure of fault zones

The impact of uterine leiomyomas: a national survey of affected women

To characterize the impact of uterine leiomyomas (fibroids) in a racially diverse sample of women in the United States

The Burden of Uterine Fibroids for African-American Women: Results of a National Survey

Uterine fibroids have a disproportionate impact on African-American women. There are, however, no data to compare racial differences in symptoms, quality of life, effect on employment, and information-seeking behavior for this disease