1,877 research outputs found
Human-Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues
By combining tissue engineering techniques with human-induced pluripotent stem cell (hiPSC) technology, human-derived engineered cardiac tissues (ECTs) have been developed using several cell lineage compositions and 3-dimensional geometries. Although hiPSC ECTs are relatively immature compared with native adult heart tissues, they have promising potential as a platform technology for drug-screening and disease modeling, and as grafts for hiPSC-based regenerative heart therapy. This chapter provides the focused overview of the current status of cardiac tissue engineering technology and its possible application
The Effelsberg-Bonn HI Survey: Data reduction
Starting in winter 2008/2009 an L-band 7-Feed-Array receiver is used for a
21-cm line survey performed with the 100-m telescope, the Effelsberg-Bonn HI
survey (EBHIS). The EBHIS will cover the whole northern hemisphere for decl.>-5
deg comprising both the galactic and extragalactic sky out to a distance of
about 230 Mpc. Using state-of-the-art FPGA-based digital fast Fourier transform
spectrometers, superior in dynamic range and temporal resolution to
conventional correlators, allows us to apply sophisticated radio frequency
interference (RFI) mitigation schemes.
In this paper, the EBHIS data reduction package and first results are
presented. The reduction software consists of RFI detection schemes, flux and
gain-curve calibration, stray-radiation removal, baseline fitting, and finally
the gridding to produce data cubes. The whole software chain is successfully
tested using multi-feed data toward many smaller test fields (1--100 square
degrees) and recently applied for the first time to data of two large sky
areas, each covering about 2000 square degrees. The first large area is toward
the northern galactic pole and the second one toward the northern tip of the
Magellanic Leading Arm. Here, we demonstrate the data quality of EBHIS Milky
Way data and give a first impression on the first data release in 2011.Comment: 17 pages, 14 figures; to be published in ApJ
Macrocerebellum: Neuroimaging and Clinical Features of a Newly Recognized Condition
Other than hamartomatous enlargement of the cerebellum as in Lhermitte-Duclos syndrome, diffuse enlargement of the cerebellum is not clearly described. We report four patients (ages 9 months to 2 years) with diffusely enlarged cerebelli as identified by measurement of the cerebellum and comparison to age appropriate normal values. The cerebellar measurements were determined in absolute numbers and expressed as ratios of cerebellum to whole brain and supratentorial brain. The clinical features of these four children (3 boys, 1 girl) consistently include global developmental delay, tone abnormalities, preserved reflexes, delayed or abnormal maturation of the visual system (oculomotor apraxia), and deficient or delayed myelination of cerebral white matter. The etiology of the macrocerebellum is unknown but we propose that the cerebellum is responding to the elaboration of growth factors intended to augment the slow development of cerebral structures. Regardless of the etiology, the finding of a macrocerebellum appears to allow the clinician to predict the clinical features of the patient and probably represents a marker for disturbed cerebral development. (J Child Neurol 1997;12:365-368).Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline
The Learning Curve for a Fetal Cardiac Intervention Team
Objectives. Multiple technical difficulties are encountered when a multidisciplinary team of subspecialists begins a minimally-invasive fetal cardiac interventional program. We describe the learning curve. Study Design. Ten pregnant sheep underwent ultrasound-guided balloon valvuloplasty of the aortic valve. Team members and their roles remained constant through the trial. The time between needle insertion and entrance of the left ventricle at the aortic root was recorded. F-test was used to assess significance (P ≤ .05). Results. The time required to accurately position the needle tip at the aortic root decreased significantly over the course of the trial, from 12 minutes with the first attempt to one minute with the last (P = .003). Conclusion. A significant learning curve is encountered when a multidisciplinary team begins a minimally-invasive fetal cardiac intervention program. However, technical proficiency can be achieved with practice. Institutions interested in developing such a program should consider practice in an animal model before proceeding to the human fetus
The shape and composition of interstellar silicate grains
We investigate the composition and shape distribution of silicate dust grains
in the interstellar medium. The effect of the amount of magnesium in the
silicate lattice is studied. We fit the spectral shape of the interstellar 10
mu extinction feature as observed towards the galactic center. We use very
irregularly shaped coated and non-coated porous Gaussian Random Field particles
as well as a statistical approach to model shape effects. For the dust
materials we use amorphous and crystalline silicates with various composition
and SiC. The results of our analysis of the 10 mu feature are used to compute
the shape of the 20 mu silicate feature and to compare this with observations.
By using realistic particle shapes we are, for the first time, able to derive
the magnesium fraction in interstellar silicates. We find that the interstellar
silicates are highly magnesium rich (Mg/(Fe+Mg)>0.9) and that the stoichiometry
lies between pyroxene and olivine type silicates. This composition is not
consistent with that of the glassy material found in GEMS in interplanetary
dust particles indicating that these are, in general, not unprocessed remnants
from the interstellar medium. Also, we find a significant fraction of SiC
(~3%). We discuss the implications of our results for the formation and
evolutionary history of cometary and circumstellar dust. We argue that the fact
that crystalline silicates in cometary and circumstellar grains are almost
purely magnesium silicates is a natural consequence of our findings that the
amorphous silicates from which they were formed were already magnesium rich.Comment: Accepted for publication in A&
Drug interactions and pharmacogenetic factors contribute to variation in apixaban concentration in atrial fibrillation patients in routine care
Factor Xa-inhibitor apixaban is an oral anticoagulant prescribed in atrial fibrillation (AF) for stroke prevention. Its pharmacokinetic profile is known to be affected by cytochrome P450 (CYP)3A metabolism, while it is also a substrate of the efflux transporters ATP-binding cassette (ABC)B1 (P-glycoprotein) and ABCG2 (breast cancer resistance protein, BCRP). In this study, we assessed the impact of interacting medication and pharmacogenetic variation to better explain apixaban concentration differences among 358 Caucasian AF patients. Genotyping (ABCG2, ABCB1, CYP3A4*22, CYP3A5*3) was performed by TaqMan assays, and apixaban quantified by mass spectrometry. The typical patient was on average 77.2 years old, 85.5 kg, and had a serum creatinine of 103.1 µmol/L. Concomitant amiodarone, an antiarrhythmic agent and moderate CYP3A/ABCB1 inhibitor, the impaired-function variant ABCG2 c.421C \u3e A, and sex predicted higher apixaban concentrations when controlling for age, weight and serum creatinine (multivariate regression; R2 = 0.34). Our findings suggest that amiodarone and ABCG2 genotype contribute to interpatient apixaban variability beyond known clinical factors
Developing cardiac and skeletal muscle share fast-skeletal myosin heavy chain and cardiac troponin-I expression
Skeletal muscle derived stem cells (MDSCs) transplanted into injured myocardium can differentiate into fast skeletal muscle specific myosin heavy chain (sk-fMHC) and cardiac specific troponin-I (cTn-I) positive cells sustaining recipient myocardial function. We have recently found that MDSCs differentiate into a cardiomyocyte phenotype within a three-dimensional gel bioreactor. It is generally accepted that terminally differentiated myocardium or skeletal muscle only express cTn-I or sk-fMHC, respectively. Studies have shown the presence of non-cardiac muscle proteins in the developing myocardium or cardiac proteins in pathological skeletal muscle. In the current study, we tested the hypothesis that normal developing myocardium and skeletal muscle transiently share both sk-fMHC and cTn-I proteins. Immunohistochemistry, western blot, and RT-PCR analyses were carried out in embryonic day 13 (ED13) and 20 (ED20), neonatal day 0 (ND0) and 4 (ND4), postnatal day 10 (PND10), and 8 week-old adult female Lewis rat ventricular myocardium and gastrocnemius muscle. Confocal laser microscopy revealed that sk-fMHC was expressed as a typical striated muscle pattern within ED13 ventricular myocardium, and the striated sk-fMHC expression was lost by ND4 and became negative in adult myocardium. cTn-I was not expressed as a typical striated muscle pattern throughout the myocardium until PND10. Western blot and RT-PCR analyses revealed that gene and protein expression patterns of cardiac and skeletal muscle transcription factors and sk-fMHC within ventricular myocardium and skeletal muscle were similar at ED20, and the expression patterns became cardiac or skeletal muscle specific during postnatal development. These findings provide new insight into cardiac muscle development and highlight previously unknown common developmental features of cardiac and skeletal muscle. © 2012 Clause et al
舞踏病ト妊娠トノ関係ニ就テ
<div><p>The role of hemodynamic forces within the embryo as biomechanical regulators for cardiovascular morphogenesis, growth, and remodeling is well supported through the experimental studies. Furthermore, clinical experience suggests that perturbed flow disrupts the normal vascular growth process as one etiology for congenital heart diseases (CHD) and for fetal adaptation to CHD. However, the relationships between hemodynamics, gene expression and embryonic vascular growth are poorly defined due to the lack of concurrent, sequential <i>in vivo</i> data. In this study, a long-term, time-lapse optical coherence tomography (OCT) imaging campaign was conducted to acquire simultaneous blood velocity, pulsatile micro-pressure and morphometric data for 3 consecutive early embryonic stages in the chick embryo. In conjunction with the <i>in vivo</i> growth and hemodynamics data, <i>in vitro</i> reverse transcription polymerase chain reaction (RT-PCR) analysis was performed to track changes in transcript expression relevant to histogenesis and remodeling of the embryonic arterial wall. Our non-invasive extended OCT imaging technique for the microstructural data showed continuous vessel growth. OCT data coupled with the PIV technique revealed significant but intermitted increases in wall shear stress (WSS) between first and second assigned stages and a noticeable decrease afterwards. Growth rate, however, did not vary significantly throughout the embryonic period. Among all the genes studied, only the MMP-2 and CASP-3 expression levels remained unchanged during the time course. Concurrent relationships were obtained among the transcriptional modulation of the genes, vascular growth and hemodynamics-related changes. Further studies are indicated to determine cause and effect relationships and reversibility between mechanical and molecular regulation of vasculogenesis.</p></div
The Simons Observatory: Magnetic Shielding Measurements for the Universal Multiplexing Module
The Simons Observatory (SO) includes four telescopes that will measure the
temperature and polarization of the cosmic microwave background using over
60,000 highly sensitive transition-edge bolometers (TES). These multichroic TES
bolometers are read out by a microwave RF SQUID multiplexing system with a
multiplexing factor of 910. Given that both TESes and SQUIDs are susceptible to
magnetic field pickup and that it is hard to predict how they will respond to
such fields, it is important to characterize the magnetic response of these
systems empirically. This information can then be used to limit spurious
signals by informing magnetic shielding designs for the detectors and readout.
This paper focuses on measurements of magnetic pickup with different magnetic
shielding configurations for the SO universal multiplexing module (UMM), which
contains the SQUIDs, associated resonators, and TES bias circuit. The magnetic
pickup of a prototype UMM was tested under three shielding configurations: no
shielding (copper packaging), aluminum packaging for the UMM, and a
tin/lead-plated shield surrounding the entire dilution refrigerator 100 mK cold
stage. The measurements show that the aluminum packaging outperforms the copper
packaging by a shielding factor of 8-10, and adding the tin/lead-plated 1K
shield further increases the relative shielding factor in the aluminum
configuration by 1-2 orders of magnitude.Comment: 7 pages, 4 figure, conference proceedings submitted to the Journal of
Low Temperature Physic
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Overview of the results of the organics PET Study of the cometary samples returned from comet Wild 2 by the Stardust mission
This presenation will provide an overview of the efforts and results produced by the Organics Preliminary Examination Team during their studies of the samples returned from comet Wild 2 by the Stardust spacecraft
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