Robert L. Brackney to Mr. Meredith (1 October 1962)

https://egrove.olemiss.edu/mercorr_pro/1418/thumbnail.jp

Pattern Recognition Residential Demand Response: An Option for Critical Peak Demand Reduction in New Zealand

Influencing households to adopt sustainable energy consumption behaviour is important to the transition towards a sustainable energy future. However, if one aims at influencing the energy consumption habits of people, one should also be able to estimate the resulting effects on the entire energy system. Residential demand response to reduce load on the electricity network has largely been impeded by information barriers and a lack of proper understanding of consumers’ behaviour. What are not well understood and are of great interest include load disaggregation, the behaviour of customers when responding to demand response request, load shifting models and their impact on the load curve of the utility. There is concern among demand response practitioners, for example, that demand response in the residential sector may simply move the peak problem with scale from one point in time to another. However, unavailability of appliance-level demand data makes it difficult to study this problem. In this paper, a generalized statistical model for generating load curves of the individual residential appliances is presented. These data allow one to identify the relative contribution of the different components of the residential load on a given residential feeder. This model has been combined with demand response survey in a neighbourhood with 400 households in Christchurch, New Zealand, to determine the effect of customers’ behaviour in reducing the neighbourhood’s winter peak demand. The results show that when customers’ are given enhanced information, they would voluntarily act to reduce their peak demand by about 10% during the morning peak hours and 11% during the evening peak hours. The demand responsiveness of the individual appliances is also presented. The effectiveness of customer behaviour modification in maintaining system reliability is also presented

Object-Oriented Database for Managing Building Modeling Components and Metadata: Preprint

Building simulation enables users to explore and evaluate multiple building designs. When tools for optimization, parametrics, and uncertainty analysis are combined with analysis engines, the sheer number of discrete simulation datasets makes it difficult to keep track of the inputs. The integrity of the input data is critical to designers, engineers, and researchers for code compliance, validation, and building commissioning long after the simulations are finished. This paper discusses an application that stores inputs needed for building energy modeling in a searchable, indexable, flexible, and scalable database to help address the problem of managing simulation input data

Selecting a Control Strategy for Plug and Process Loads

Plug and Process Loads (PPLs) are building loads that are not related to general lighting, heating, ventilation, cooling, and water heating, and typically do not provide comfort to the building occupants. PPLs in commercial buildings account for almost 5% of U.S. primary energy consumption. On an individual building level, they account for approximately 25% of the total electrical load in a minimally code-compliant commercial building, and can exceed 50% in an ultra-high efficiency building such as the National Renewable Energy Laboratory's (NREL) Research Support Facility (RSF) (Lobato et al. 2010). Minimizing these loads is a primary challenge in the design and operation of an energy-efficient building. A complex array of technologies that measure and manage PPLs has emerged in the marketplace. Some fall short of manufacturer performance claims, however. NREL has been actively engaged in developing an evaluation and selection process for PPLs control, and is using this process to evaluate a range of technologies for active PPLs management that will cap RSF plug loads. Using a control strategy to match plug load use to users' required job functions is a huge untapped potential for energy savings

Aedes aegypti uses RNA interference in defense against Sindbis virus infection

<p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) is an important anti-viral defense mechanism. The <it>Aedes aegypti </it>genome encodes RNAi component orthologs, however, most populations of this mosquito are readily infected by, and subsequently transmit flaviviruses and alphaviruses. The goal of this study was to use <it>Ae. aegypti </it>as a model system to determine how the mosquito's anti-viral RNAi pathway interacts with recombinant Sindbis virus (SINV; family <it>Togaviridae</it>, genus <it>Alphavirus</it>).</p> <p>Results</p> <p>SINV (TR339-eGFP) (+) strand RNA, infectious virus titers and infection rates transiently increased in mosquitoes following dsRNA injection to cognate <it>Ago2</it>, <it>Dcr2</it>, or <it>TSN </it>mRNAs. Detection of SINV RNA-derived small RNAs at 2 and 7 days post-infection in non-silenced mosquitoes provided important confirmation of RNAi pathway activity. Two different recombinant SINV viruses (MRE16-eGFP and TR339-eGFP) with significant differences in infection kinetics were used to delineate vector/virus interactions in the midgut. We show virus-dependent effects on RNAi component transcript and protein levels during infection. Monitoring midgut <it>Ago2</it>, <it>Dcr2</it>, and <it>TSN </it>transcript levels during infection revealed that only <it>TSN </it>transcripts were significantly increased in midguts over blood-fed controls. Ago2 protein levels were depleted immediately following a non-infectious bloodmeal and varied during SINV infection in a virus-dependent manner.</p> <p>Conclusion</p> <p>We show that silencing RNAi components in <it>Ae. aegypti </it>results in transient increases in SINV replication. Furthermore, <it>Ae. aegypti </it>RNAi is active during SINV infection as indicated by production of virus-specific siRNAs. Lastly, the RNAi response varies in a virus-dependent manner. These data define important features of RNAi anti-viral defense in <it>Ae. aegypti</it>.</p

Perinatal Nurses Respond To Shared Decision-Making Education: A Quasi-Experimental Study

Women describe a loss of autonomy during childbirth as a contributing factor to labor dissatisfaction. Shared decision-making with choice, option, and decision talk may improve satisfaction. Nurses (n = 29) received education on supporting women's autonomy with a standardized communication tool (SUPPORT) to facilitate shared decision-making and create an evolving birth plan. This quasi-experimental pre-/post-test design evaluated participant responses to the education module. Participants supported the use of the SUPPORT tool for shared decision-making and developing evolving birth plans. Most recommended initiation between 13- and 26-weeks' gestation. Nurses' willingness to advocate for women's autonomy increased significantly after education (p = .022). Shared decision-making with standardized perinatal communication may support a woman's perinatal education and her satisfaction with labor. Women enter the hospital birthing center with expectations for their labor experience (Cook &amp; Loomis, 2012). Postpartum women report greater satisfaction with their labor and birth experience when their goals are met; they have a voice in their care and they participate in shared decision- making (Reed et al., 2017). Additionally, evidence suggests that mothers and their newborns have better mental and physical outcomes when the mother's labor expectations and goals are achieved (Hidalgo-Lopezosa et al., 2017). Shared decision-making benefits from facilitated communication between the laboring woman, nurses, and others involved in her care. The SUPPORT tool was created to involve the expectant mother in shared decision- making. Any women's health-care provider can use the SUPPORT tool to facilitate communication in preparation for labor and birth. Specifically, a registered nurse (RN) can use the SUPPORT tool to discuss and educate pregnant women about interventions commonly used during labor by the health-care team and laboring women. To promote shared decision-making with the use of the SUPPORT tool, an educational module designed for women's health nurses was developed. This pre-/post-test quasi-experimental research study reports on the women's health nurses' responses to the education module and the nurses' recommendations for the use of the SUPPORT tool in practice

Limited influence of the microbiome on the transcriptional profile of female Aedes aegypti mosquitoes

The microbiome is an assemblage of microorganisms living in association with a multicellular host. Numerous studies have identified a role for the microbiome in host physiology, development, immunity, and behaviour. The generation of axenic (germ-free) and gnotobiotic model systems has been vital to dissecting the role of the microbiome in host biology. We have previously reported the generation of axenic Aedes aegypti mosquitoes, the primary vector of several human pathogenic viruses, including dengue virus and Zika virus. In order to better understand the influence of the microbiome on mosquitoes, we examined the transcriptomes of axenic and conventionally reared Ae. aegypti before and after a blood meal. Our results suggest that the microbiome has a much lower effect on the mosquito's gene expression than previously thought with only 170 genes influenced by the axenic state, while in contrast, blood meal status influenced 809 genes. The pattern of expression influenced by the microbiome is consistent with transient changes similar to infection rather than sweeping physiological changes. While the microbiome does seem to affect some pathways such as immune function and metabolism, our data suggest the microbiome is primarily serving a nutritional role in development with only minor effects in the adult

Molecular epidemiology of Powassan virus in North America

Powassan virus (POW) is a tick-borne flavivirus distributed in Canada, the northern USA and the Primorsky region of Russia. POW is the only tick-borne flavivirus endemic to the western hemisphere, where it is transmitted mainly between Ixodes cookei and groundhogs (Marmota monax). Deer tick virus (DTV), a genotype of POW that has been frequently isolated from deer ticks (Ixodes scapularis), appears to be maintained in an enzootic cycle between these ticks and white-footed mice (Peromyscus leucopus). DTV has been isolated from ticks in several regions of North America, including the upper Midwest and the eastern seaboard. The incidence of human disease due to POW is apparently increasing. Previous analysis of tick-borne flaviviruses endemic to North America have been limited to relatively short genome fragments. We therefore assessed the evolutionary dynamics of POW using newly generated complete and partial genome sequences. Maximum-likelihood and Bayesian phylogenetic inferences showed two well-supported, reciprocally monophyletic lineages corresponding to POW and DTV. Bayesian skyline plots based on year-of-sampling data indicated no significant population size change for either virus lineage. Statistical model-based selection analyses showed evidence of purifying selection in both lineages. Positive selection was detected in NS-5 sequences for both lineages and envelope sequences for POW. Our findings confirm that POW and DTV sequences are relatively stable over time, which suggests strong evolutionary constraint, and support field observations that suggest that tick-borne flavivirus populations are extremely stable in enzootic foci

Gene silencing in tick cell lines using small interfering or long double-stranded RNA

Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for RNAi-mediated gene silencing in tick cells need to be established. Ten cell lines from four economically important ixodid tick genera (Amblyomma, Hyalomma, Ixodes and Rhipicephalus including the sub-species Boophilus) were used to examine key parameters including small interfering RNA (siRNA), double stranded RNA (dsRNA), transfection reagent and incubation time for silencing virus reporter and endogenous tick genes. Transfection reagents were essential for the uptake of siRNA whereas long dsRNA alone was taken up by most tick cell lines. Significant virus reporter protein knockdown was achieved using either siRNA or dsRNA in all the cell lines tested. Optimum conditions varied according to the cell line. Consistency between replicates and duration of incubation with dsRNA were addressed for two Ixodes scapularis cell lines; IDE8 supported more consistent and effective silencing of the endogenous gene subolesin than ISE6, and highly significant knockdown of the endogenous gene 2I1F6 in IDE8 cells was achieved within 48 h incubation with dsRNA. In summary, this study shows that gene silencing by RNAi in tick cell lines is generally more efficient with dsRNA than with siRNA but results vary between cell lines and optimal parameters need to be determined for each experimental system