Fixation of the biological samples

Cílem diplomové práce je seznámit se s možností fixace biologických vzorků. Vybrat a fixovat vhodnou tkáň pomocí formaldehydu a dalšího fixačního prostředku. V neposlední řadě provést měření pro zjištění vlivu formaldehydu a dalšího fixačního prostředku na relaxační vlastnosti daného biologického vzorku. Myší mozky byly vybrány jako vhodná tkáň pro fixaci. Měření vlivu formaldehydu a dalšího fixačního prostředku na relaxační vlastnosti tkáně probíhalo na Ústavu přístrojové techniky Akademie věd České republiky.The aim of thesis is acquaint with possibilities of fixation biological tissues. Choose a suitable tissue and fix it with formaldehyde and another fixator. Last but not least make measurements which determine effects of formaldehyde on the relaxation time of biological tissues. Mice brains were chosen as a suitable for tissues fixation. Measuring effects of formaldehyde and another fixator to relaxation time carried out at the Institute of Scientific Instuments Academy of Sciences of the Czech Republic.

Chart of electromagnetic fields in GIS

Cílem bakalářské práce je seznámit se s existencí elektromagnetických polí v životním prostředí a s hygienickým předpisem pro účinky těchto polí. Dále seznámení se s funkcemi měřiče EMR-30 a seznámení se s programovým prostředím geografického informačního systému ArcView a jeho možnostmi při zpracování dat. Měření elektromagnetických polí probíhalo v terénu v okolí kolejí Pod Palackého vrchem. Pro zpracování získaných dat, tvorbu map a následnou analýzu byly využity programy ArcScene 10.1, ArcMap 10.1 a jejich nadstavby.The main goal in this bachelor thesis was to study the existence of electromagnetic fields in enviroment, and to study hygienic regulations. Then, to study functions of measurment system EMR-30 and to study UI of geographic information system ArcView and it´s abilities for data processing. Measurement of electromagnetic fields was carried out in the neighborhood of campus Pod Palackeho vrchem. For processing the measured data, creating maps and subsequent analysis were used programs ArcMap 10.1, ArcScene 10.1 and add-ons.

Customer Behaviour Analysis on the Meat Products Market

Import 04/11/2015Předmětem bakalářské práce Analýza nákupního chování zákazníků na trhu masa a masných výrobků je analýza nákupního chování ve vybraném regionu a jeho zhodnocení. Teoretická část pojednává o trhu, nákupním chování a typech spotřebitelů. Praktická část je zaměřena na zhodnocení nákupního chování daného regionu. V závěru je popsané shrnutí celé práce a zhodnocení.The subject of this bachelor thesis “Analysis of buying behavior of customers in the market of meat and meat products” is the analysis of shopping behavior in the selected region and its evaluation. The theoretical part discusses the market, purchasing patterns, and types of consumers. The practical part is focused on evaluation of shopping behavior in the region. In conclusion there is described a summary of this thesis and evaluation.In conclusion there is described a summary and evaluation of this thesis.116 - Katedra marketingu a obchodudobř

Party System Development in the Faroe Islands

The article deals with the creation of and changes in the party system of the Faroe Islands. Sartori’s party system typology is used. Attention is also paid to the cleavages shaping the Faroese party system. The story of Faroese party politics starts at the beginning of the 20th century when the Unionist and Self-Government parties were founded. The system was influenced mainly by Faroese-Danish relations and by economic crises in the 1920s and 1990s

Aging restricts the ability of mesenchymal stem cells to promote the generation of oligodendrocytes during remyelination.

Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) that leads to severe neurological deficits. Due to their immunomodulatory and neuroprotective activities and their ability to promote the generation of oligodendrocytes, mesenchymal stem cells (MSCs) are currently being developed for autologous cell therapy in MS. As aging reduces the regenerative capacity of all tissues, it is of relevance to investigate whether MSCs retain their pro-oligodendrogenic activity with increasing age. We demonstrate that MSCs derived from aged rats have a reduced capacity to induce oligodendrocyte differentiation of adult CNS stem/progenitor cells. Aging also abolished the ability of MSCs to enhance the generation of myelin-like sheaths in demyelinated cerebellar slice cultures. Finally, in a rat model for CNS demyelination, aging suppressed the capability of systemically transplanted MSCs to boost oligodendrocyte progenitor cell (OPC) differentiation during remyelination. Thus, aging restricts the ability of MSCs to support the generation of oligodendrocytes and consequently inhibits their capacity to enhance the generation of myelin-like sheaths. These findings may impact on the design of therapies using autologous MSCs in older MS patients.The authors would like to thank the following funding agencies for their support: Paracelsus Medical University PMU-FFF Long-Term Fellowship L-12/01/001-RIV (to and Stand-Alone Grant E-12/15/077-RIT (both to F.J.R.); Chilean Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) FONDECYT Program Regular Grant Nº 1161787 (to F.J.R.), Regular Grant Nº 1141015 (to L.F.B.); Chilean CONICYT PCI Program Grant Nº REDES170233 (to F.J.R.), Grant Nº REDES180139 and Grant Nº REDI170037; Chilean CONICYT FONDEFIDeA Program Grant Nº ID17AM0043 (to M.E.S. and F.J.R.); European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreements N HEALTH-F2-2011-278850 (INMiND) and HEALTH-F2-2011-279288 (IDEA). The work in the Küry laboratory was supported by the German Research Foundation (DFG; KU1934/2_1, KU1934/5-1) and the Christiane and Claudia Hempel Foundation for clinical and iBrain. The work in the Franklin laboratory was supported by grants from the UK Multiple Sclerosis Society and the Adelson Medical Research Foundation, and a core support grant from the Wellcome Trust and MRC to the Wellcome-MRC Cambridge Stem Cell Institute. In addition, the present work was supported by the state of Salzburg (to L.A.). We thank Armin Schneider, Sygnis Pharma AG Heidelberg, Germany, for the MBP promoter construct. We disclose any conflict of interest

The PI3-kinase delta inhibitor idelalisib (GS-1101) targets integrin-mediated adhesion of chronic lymphocytic leukemia (CLL) cell to endothelial and marrow stromal cells

CLL cell trafficking between blood and tissue compartments is an integral part of the disease process. Idelalisib, a phosphoinositide 3-kinase delta (PI3K\u3b4) inhibitor causes rapid lymph node shrinkage, along with an increase in lymphocytosis, prior to inducing objective responses in CLL patients. This characteristic activity presumably is due to CLL cell redistribution from tissues into the blood, but the underlying mechanisms are not fully understood. We therefore analyzed idelalisib effects on CLL cell adhesion to endothelial and bone marrow stromal cells (EC, BMSC). We found that idelalisib inhibited CLL cell adhesion to EC and BMSC under static and shear flow conditions. TNF\u3b1-induced VCAM-1 (CD106) expression in supporting layers increased CLL cell adhesion and accentuated the inhibitory effect of idelalisib. Co-culture with EC and BMSC also protected CLL from undergoing apoptosis, and this EC- and BMSC-mediated protection was antagonized by idelalisib. Furthermore, we demonstrate that CLL cell adhesion to EC and VLA-4 (CD49d) resulted in the phosphorylation of Akt, which was sensitive to inhibition by idelalisib. These findings demonstrate that idelalisib interferes with integrin-mediated CLL cell adhesion to EC and BMSC, providing a novel mechanism to explain idelalisib-induced redistribution of CLL cells from tissues into the blood

Evaluating a Targeted Cancer Therapy Approach Mediated by RNA

Conventional anti-cancer therapies based on chemo- and/or radiotherapy represent highly effective means to kill cancer cells but lack tumor specificity and, therefore, result in a wide range of iatrogenic effects. A promising approach to overcome this obstacle is spliceosome-mediated RNA trans-splicing (SMaRT), which can be leveraged to target tumor cells while leaving normal cells unharmed. Notably, a previously established RNA trans-splicing molecule (RTM44) showed efficacy and specificity in exchanging the coding sequence of a cancer target gene (Ct-SLCO1B3) with the suicide gene HSV1-thymidine kinase in a colorectal cancer model, thereby rendering tumor cells sensitive to the prodrug ganciclovir (GCV). In the present work, we expand the application of this approach, using the same RTM44 in aggressive skin cancer arising in the rare genetic skin disease recessive dystrophic epidermolysis bullosa (RDEB). Stable expression of RTM44, but not a splicing-deficient control (NC), in RDEB-SCC cells resulted in expression of the expected fusion product at the mRNA and protein level. Importantly, systemic GCV treatment of mice bearing RTM44-expressing cancer cells resulted in a significant reduction in tumor volume and weight compared with controls. Thus, our results demonstrate the applicability of RTM44-mediated targeting of the cancer gene Ct-SLCO1B3 in a different malignancy