Effects of Rhizobacteria Application on Leaf and Fruit Nutrient Content of Different Apple Scion–Rootstock Combinations

The plant pomological characteristics and physiological behaviors of genotypes in modern apple cultivation could be different depending on the use of rootstock, changing growth ecology and application of biological control agents. The aim of this research was to determine the effects of rhizobacteria application on leaf and fruit nutrient contents in different apple scion–rootstock combinations. This study was carried out with seven standard cultivars (Scarlet Spur, Red Chief, Fuji, Jeromine, Galaxy Gala, Granny Smith, and Golden Reinders) budded on M.9 and MM.106 rootstocks. In the experiment, trees were sprayed by a nitrogen + phosphorus solvent rhizobacteria three times, with an interval of 15 days in the spring period. The effect of rhizobacteria application on leaf and fruit nutrient contents was statistically significant and provided generally significant positive contributions, except for leaf Mg content. Comparing both rootstocks, the positive effect of bacterial application was higher on the M.9 rootstock for leaf N and B content and fruit N and Fe content, and on the MM.106 rootstock for other nutrient content. While the effects of bacterial application on the basis of cultivars were generally positive, the highest positive contribution was made in leaf P content (10.7%) and fruit Mn content (32.1%) of the Fuji cultivar. Considering the total increase in nutrients in scion–rootstocks combination, rhizobacteria application had a positive effect on the leaf nutrient contents in Golden Reinders/MM.106, but not leaf K content. The highest increases in leaves of scion-rootstock combinations were determined as 4.0% in N content in Granny Smith/M.9, 14.1% in P content in Scarlet Spur/MM.106, 7.1% in K content in Fuji/MM.106, 4.4% in Ca content in Jeromine/M.9, and 14.0% in Mg content in Granny Smith/MM.106. The highest increase in fruit nutrient contents was between 4.9% (N content) and 13.5% (Ca content) for macro elements, and between 9.5% (Cu content) and 41.8% (Mn content) for microelements. The results of the present study may provide significant leads for further studies on this subject.Effects of Rhizobacteria Application on Leaf and Fruit Nutrient Content of Different Apple Scion–Rootstock CombinationspublishedVersio

Assessment of Morphological Traits, Nutritional and Nutraceutical Composition in Fruits of 18 Apricot cv. Sekerpare Clones

Apricot (Prunus armeniaca L.) is one of the most important members of Prunus and its trees bears delicious and nutritious fruits during summer months in the temperate zones in the world. Apricot cultivars are propagated asexually which consists of clones. Information on inter-clonal variations in apricot cultivars can assist us in the selection of better clones from commercial cultivars. We aimed to determine morphological traits (fruit weight, seed weight, kernel weight, flesh/seed ratio, shape index, fruit firmness, color index), nutritional (sugars and organic acids) and nutraceutical (total phenolic, total flavonoids, total carotenoid and antioxidant activity) composition of 18 clones of Sekerpare apricot cultivar grown together in Kagizman district in eastern Turkey. Results showed significant differences among clones concerning most of the morphological traits, nutritional and nutraceutical compositions. Fruit weight, flesh/seed ratio and fruit firmness of clones were in range of 23.14–27.11 g, 11.21–13.14 and 3.88–5.11 kg/cm2, respectively. Fruit shape index was slightly similar among all clones which was between 0.95 and 1.03. Citric acid and sucrose were found to be the predominant organic acid and sugar among clones which varied from 728 to 915 mg/100 g and 7.11 to 9.94 g/100 g, respectively. The clone ‘KS2’ exhibited the highest level of total phenol (67.1 mg gallic acid equivalent per 100 g) and antioxidant activity (2.16 μmol trolox equivalent per g). The study confirmed the diversity among Sekerpare clones and effectiveness of combining morphological, nutritional and nutraceutical analyses in assessment of Şekerpare clones and its use for future pre-breeding programs

INSECTICIDAL EFFECTS OF ESSENTIAL OILS OBTAINED FROM SIX PLANTS AGAINST CALLOSOBRUCHUS MACULATUS (F.) (COLEOPTERA: BRUCHIDAE), A PEST OF COWPEA (VIGNA UNGUICULATA) (L.)

WOS: 000381779500042Callosobruchus maculatus (F.), cowpea seed beetle (Coleoptera: Bruchidae) is one of the most serious pests of cowpea (the black-eyed pea) grains, worldwide. In this study, essential oils of Artemisia dracunculus L., Artemisia santonicum L., Artemisia spicigera C. Koch, Origanum onites L., Satureja thymbra L. and Thymus sipyleus Boiss. were tested for their insecticidal activities against three day-old adults of C. maculatus at 25 +/- 2 degrees C, 65 +/- 5 % r.h. in dark conditions, at different exposure times (12, 24, 48 and 72 h), and doses (5, 7.5 and 10 mu l). It was found that there were the mortalities in all exposure doses and durations. The percentage mortality of adults of C. maculatus increased with increasing the concentration of different oils and exposure times. Minimum mortality rate in C. maculatus adults (16.6%) was recorded at 12 h with 5 mu l essential oil of O. onites, while the peak mortality was registered as 100%, at 72 h with 10 mu l essential oil of A. dracunculus. Additionally, LD25, LD50 and LD90 values of each essential oil were estimated for C. maculatus. Results suggested that the essential oils from the tested plants could be used as potential control agents against C. maculatus adults in stored cowpea (the black-eyed pea) protection

Chemical Composition of Seed Propagated Chestnut Genotypes from Northeastern Turkey

Bozhuyuk, Mehmet Ramazan/0000-0001-5021-6019; Akbulut, Mustafa/0000-0003-1028-162XWOS: 000411504400015Turkey ranked third place in the world for chestnut production after China and Bolivia and the country has unique chestnut populations including valuable and diverse seed propagated chestnut genotypes. in this study, chestnuts (Castanea sativa) were collected during the 2016 harvest season from Northeastern part of Turkey from promising 12 different seedling origin genotypes. Tree growth habit, nut weight, kernel ratio, kernel color, moisture, crude protein, crude fat, dietary fiber, total polyphenols, antioxidant activity and fatty acid content of fruits belongs to 12 genotypes were determined. the results showed that, the majority of genotypes had semi upright tree growth habit. the nut weight and kernel ratio were between 5.05 g (K-10) and 10.10 g (K-5) and 71.10% (K-1) and 82.44% (K-3) among genotypes. the total crude fat content ranged from 0.87% (K-7) to 2.61% (K-1) while the crude protein ranged from 4.80% (K-7) to 7.65% (K-1). the dietary fiber content made up no more than 3.61% of the remaining portion of the kernel. It was found that total polyphenols was between 1.66 and 2.70 g GAE/ kg and antioxidant activity was between 5.80 and 9.07 mu mol Trolox equivalent/ g dry weight basis. Oleic and linoleic acid were the major fatty acids in all chestnut fruits and followed by palmitic and linolenic acid. the results showed that there was enough variations among seed propagated chestnut genotypes for most of the searched parameters and this highlights the importance of conserving the genotypes, as their high levels of heterogeneity allow them to respond to abiotic and biotic stresses and adapt to low-input farming systems

MOLECULAR CHARACTERIZATION OF WILD CAROB (Ceratonia Siliqua L.) GENOTYPES BY SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) TECHNIQUES IN TURKEY MOLEKULARNA KARAKTERIZACIJA GENOTIPOVA DIVLJEG ROGAČA (Ceratonia Siliqua L.) SRAP TEHNIKOM U TURSKOJ

© 2022,Genetika. All Rights ReservedCarob (Ceratonia siliqua L.) with limited widespread in Turkey is considered as secondary forest tree. In this study, molecular characterizations were made for 508 genotypes of seven different carob populations colelcted from Eagean, Western and Eastern Mediterranean regions of Turkey with the aid of sequence-related amplified polymorphism (SRAP) technique. Identification of wild carob genotypes, relativity levels and genetic variations among them were performed. Genetic similarities among 508 wild carob genotypes collected from Eagean, Western and Eastern Mediterranean regions of Turkey varied between 0.20-1.00 and there was a large variation among the genotypes. The genetic similarities among 250 wild carob genotypes collected from Aegean region varied between 0.36-1.00. The genetic similarities among 154 wild carob genotypes collected from Western Mediterranean region varied between 0.23-1.00. The genetic similarities among 102 wild carob genotypes collected from Eastern Mediterranean region varied between 0.21-1.00. Through the molecular analyses conducted with SRAP primers, besides the large variations among the entire genotypes, large variations were also observed between the genotypes of different regions. With this study, genetic variations were put forth among the wild carob genotypes naturally growing in different regions of Turkey. It was concluded based on present findings that marker system could reliably be used to put forth genetic variations among wild carob genotypes

Seed-Propagated Summer Apples: Great Morphological and Biochemical Diversity

The northeastern Anatolia region of Turkey is one of the richest plant biodiversity areas in Turkey. This study describes some important tree and fruit characteristics of 20 seed-propagated summer apple genotypes from northeastern Anatolia using morphological and biochemical markers. The genotypes were evaluated morphologically for tree growth habit, ripening date, fruit weight, fruit firmness, fruit shape, fruit color, fruit taste, juiciness and aroma. The biochemical characteristics measured were organic acids, SSC (soluble solid content), titratable acidity, vitamin C, total phenolic content and antioxidant capacity. We found significant differences for most of the morphological and biochemical characteristics. The genotypes exhibited quite diverse harvest dates ranging from 4 July to 16 August. Summer apple genotypes had fruit weight between 101.9 g and 133.5 g. The genotypes had diverse fruit shapes, including round, conic and oblate, and over fruit colours such as pink, red, yellow and green. Genotypes GUM7 and GUM13 stood out for fruit weight, SSC, total phenolic and antioxidant capacity. GUM7 and GUM13 present dissimilarity from the other genotypes. While the first showed a lower average fruit weight, SSC, total phenolic content and antioxidant capacity, the second revealed higher average of fruit weight, total phenolic content, antioxidant capacity and higher SSC. Due to their favorable characteristics, GUM13 can be used for further crosses with genotypes of other groups, which also present favorable characteristics

Effects of Rhizobacteria Application on Leaf and Fruit Nutrient Content of Different Apple Scion–Rootstock Combinations

The plant pomological characteristics and physiological behaviors of genotypes in modern apple cultivation could be different depending on the use of rootstock, changing growth ecology and application of biological control agents. The aim of this research was to determine the effects of rhizobacteria application on leaf and fruit nutrient contents in different apple scion–rootstock combinations. This study was carried out with seven standard cultivars (Scarlet Spur, Red Chief, Fuji, Jeromine, Galaxy Gala, Granny Smith, and Golden Reinders) budded on M.9 and MM.106 rootstocks. In the experiment, trees were sprayed by a nitrogen + phosphorus solvent rhizobacteria three times, with an interval of 15 days in the spring period. The effect of rhizobacteria application on leaf and fruit nutrient contents was statistically significant and provided generally significant positive contributions, except for leaf Mg content. Comparing both rootstocks, the positive effect of bacterial application was higher on the M.9 rootstock for leaf N and B content and fruit N and Fe content, and on the MM.106 rootstock for other nutrient content. While the effects of bacterial application on the basis of cultivars were generally positive, the highest positive contribution was made in leaf P content (10.7%) and fruit Mn content (32.1%) of the Fuji cultivar. Considering the total increase in nutrients in scion–rootstocks combination, rhizobacteria application had a positive effect on the leaf nutrient contents in Golden Reinders/MM.106, but not leaf K content. The highest increases in leaves of scion-rootstock combinations were determined as 4.0% in N content in Granny Smith/M.9, 14.1% in P content in Scarlet Spur/MM.106, 7.1% in K content in Fuji/MM.106, 4.4% in Ca content in Jeromine/M.9, and 14.0% in Mg content in Granny Smith/MM.106. The highest increase in fruit nutrient contents was between 4.9% (N content) and 13.5% (Ca content) for macro elements, and between 9.5% (Cu content) and 41.8% (Mn content) for microelements. The results of the present study may provide significant leads for further studies on this subject

Morphological and biochemical diversity in fruits of unsprayed Rosa canina and Rosa dumalis ecotypes found in different agroecological conditions

The Rosa is one of the most diverse genera in the plant kingdom and, in particular, its fruits have been used for multiple purposes in different parts of the world for centuries. Within the genus, Rosa canina and Rosa dumalis are, economically, the most important species and dominate Rosa fruit production. In this study, some important fruit and shrub traits of ten Rosa canina and ten Rosa dumalis ecotypes collected from rural areas of Kars province, located in the east Anatolia region of Turkey were investigated. We found significant differences among ecotypes in most of the morphological and biochemical traits. The ecotypes were found between 1446–2210 m altitude. Fruit weight and fruit flesh ratio ranged from 2.95 g to 4.72 g and 62.55% to 74.42%, respectively. SSC (Soluble Solid Content), Vitamin C, total phenolic, total flavonoid, total carotenoid, and total anthocyanin content of the ecotypes ranged from 16.9–22.7%, 430–690 mg per 100 g FW (fresh weight), 390–532 mg gallic acid equivalent per 100 g FW, 0.88–2.04 mg per g FW, 6.83–15.17 mg per g FW and 3.62–7.81 mg cyanidin-3-glucoside equivalent per kg, respectively. Antioxidant activity was determined to be between 19.7–34.7 mg ascorbic acid equivalent per g fresh weight. Rosa ecotypes contained chlorogenic acid and rutin the most as phenolic compound. Our results indicated great diversity within both R. canina and R. dumalis fruits

Quality Attributes of Chitosan-Coated Cornelian Cherry (<i>Cornus mas</i> L.) Fruits under Different Storage Temperatures

Temperature is the dominant environmental stimulus that influences the postharvest quality, visual appearance, and nutritional content of fruits. Temperature hastens fruit ripening and senescence by the impact on respiration rate and the acceleration of metabolic reactions. This study was conducted to select the optimized temperature for preserving the quality-related traits and antioxidant potential of cornelian cherry fruits after harvest. The fruits were treated with 1% chitosan and then kept under 0, 5, 10, and 21 °C for 21 days. The results revealed that fruits kept under lower than room temperature (21 °C) better retained antioxidant capacity and had higher levels of phenolics, flavonoids, and anthocyanins and even higher antioxidant enzyme activity, hence attaining prolonged postharvest life. Considering the quantity attributes and the shelf life, the temperature of choice was 0 °C. Zero temperature was also the best to keep the antioxidant capacity of cornelian cherry fruits. Overall, the results showed that low temperature and chitosan pretreatment provide an efficient method for maintaining the nutritional quality and antioxidant capacity of cornelian cherry fruits during storage time

Quality attributes of chitosan-coated cornelian cherry (Cornus mas l.) fruits under different storage temperatures

Temperature is the dominant environmental stimulus that influences the postharvest quality, visual appearance, and nutritional content of fruits. Temperature hastens fruit ripening and senescence by the impact on respiration rate and the acceleration of metabolic reactions. This study was conducted to select the optimized temperature for preserving the quality-related traits and antioxidant potential of cornelian cherry fruits after harvest. The fruits were treated with 1% chitosan and then kept under 0, 5, 10, and 21◦C for 21 days. The results revealed that fruits kept under lower than room temperature (21◦C) better retained antioxidant capacity and had higher levels of phenolics, flavonoids, and anthocyanins and even higher antioxidant enzyme activity, hence attaining prolonged postharvest life. Considering the quantity attributes and the shelf life, the temperature of choice was 0◦C. Zero temperature was also the best to keep the antioxidant capacity of cornelian cherry fruits. Overall, the results showed that low temperature and chitosan pretreatment provide an efficient method for maintaining the nutritional quality and antioxidant capacity of cornelian cherry fruits during storage time. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Ministerstvo Zemědělství; Mendelova Univerzita v Brně, MENDELU: 51834/2017-MZE-17253, 6.2.10University of Maragheh; Urmia University, Iran; Mendel University in Brno, Czech Republic; [6.2.10]; [51834/2017-MZE-17253