10 research outputs found
Development and validation of high-density SNP array in ducks
Development and validation of high-density SNP array in ducks. XIth European symposium on poultry genetics (ESPG
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We consider a random variable Y and approximations Yn, n â N, defined on the same probability space with values in the same measurable space as Y. We are interested in situations where the approximations Yn allow to define a Dirichlet form in the space L 2 (PY) where PY is the law of Y. Our approach consists in studying both biases and variances. The article attempts to propose a general theoretical framework. It is illustrated by severa
GDC Buffer Layer Synthesized by Reactive Magnetron Sputtering: Effect of Total Pressure and Thickness on SOFC Performances
International audienceGadolinia-doped ceria (GDC) buffer layers were synthesized by reactive magnetron sputtering under different total pressures and different thickness. All as-deposited and after an annealing treatment during two hours under air at 1000°C coating presents a face centered cubic (f.c.c) structure of ceria with dense and adhesive morphology. The cell synthesized under 0.1 Pa and 0.57 ”m present the best performances. (open-circuit voltage (OCV): 1.133 eV and power density: 1650 mW.cm-ÂČ @ 800 mA.cm-ÂČ at 790°C)
DĂ©veloppement dâune puce de gĂ©notypage haute densitĂ© 600K pour le canard commun et le canard de Barbarie
National audienceGenomic selection is widely used for genetic improvement of many plant and animal species. This evaluation method could potentially increase genetic gain in ducks for traits that cannot be measured on selection candidates (lethal, expressed in one sex only, or measured on hybrid duck for purebred selection). Implementation of genomic selection depends on the availability of genotyping tools, such as SNP chips, but so far none were developed neither for common duck (Anas platyrhynchos) nor Muscovy duck (Cairina moschata). This paper describes the assembly of the Muscovy duck genome (the common duck genome is already available), and the design of a 600K Thermo Fisher SNP chip. The common duck genome was used as reference for the assembly of the Muscovy genome: 3702 scaffolds were produced, with a N50 of 2.4 Mb. SNP were identified from sequence data: several Muscovy and common ducks populations (Rouen duck, Mallard duck, Pekin duck), were collected, and 50 samples were pooled independently for each population. After raw quality controls, 8.4 million SNPs and 12.2 million SNP were identified for Muscovy and common ducks respectively. Filters based on Minor Allele Frequence and genome coverage were used, and finally 343 950 SNP were kept for the common duck, and 331 241 SNP for the Muscovy duck.La sĂ©lection gĂ©nomique est aujourdâhui largement utilisĂ©e pour lâamĂ©lioration gĂ©nĂ©tique des animaux et des plantes. Chez le canard, cette mĂ©thode pourrait permettre dâamĂ©liorer le progrĂšs gĂ©nĂ©tique pour des caractĂšres non-mesurables sur les candidats Ă la sĂ©lection (lĂ©taux, exprimĂ©s chez un seul sexe, ou mesurĂ©s sur lâhybride pour la sĂ©lection des lignĂ©es pures). MalgrĂ© lâintĂ©rĂȘt potentiel de lâutilisation de la sĂ©lection gĂ©nomique chez le canard, aucun outil moderne de gĂ©notypage nâest disponible pour les canards commun (Anas platyrhynchos) et de Barbarie (Cairina moschata). Cette Ă©tude dĂ©crit la finalisation de lâassemblage du gĂ©nome du canard de Barbarie, et la mise au point dâun outil de gĂ©notypage haut-dĂ©bit et haute-densitĂ© pour les 2 espĂšces : une puce 600K Thermo Fisher SNP. Lâassemblage du gĂ©nome du Barbarie a permis dâobtenir 3702 scaffolds avec un N50 de 2,4 Mb. Les scaffolds ont ensuite Ă©tĂ© ordonnĂ©s en chromosomes, par alignement sur le gĂ©nome du canard commun. Pour lâidentification des SNP, des pools (mĂ©langes) dâADN de canards colvert, de Rouen, ainsi que de lignĂ©es commerciales de canards PĂ©kin et de Barbarie ont Ă©tĂ© sĂ©quencĂ©s. Les sĂ©quences ont Ă©tĂ© alignĂ©es sur les gĂ©nomes de rĂ©fĂ©rence correspondant. A lâissue des contrĂŽles sur la qualitĂ©, 8,4 et 12,2 millions de SNP avec une Minor Allele Frequency (MAF) â„ 0,05 ont Ă©tĂ© identifiĂ©s pour le canard de Barbarie et pour le canard commun respectivement. Les SNP ont Ă©tĂ© filtrĂ©s afin de maximiser le nombre de marqueurs informatifs par population et dâassurer une bonne couverture du gĂ©nome. A lâissue de ces tris, 343 950 SNP dâune part et 331 241 SNP dâautre part ont Ă©tĂ© choisis pour les canards commun et de Barbarie
Practical applications of genomic assignment methods in the avian breeding industry
Practical applications of genomic assignment methods in the avian breeding industry. XIth European symposium on poultry genetics (ESPG