Allelic variation observed at one microsatellite locus between the two synonym grape cultivars Black Currant and Mavri Corinthiaki

Research NoteBlack Currant and Mavri Corinthiaki are considered synonyms for Corinthe Noir, a parthenocarpic seedless raisin cultivar. No differences between them were found by RAPD analysis with 1 1 primers. They also have the same genotype in 15 out of 16 microsatellite loci, demonstrating the Black Currant and Mavri Corinthiaki are essentially identical. Nevertheless, a difference was observed in one allele at one locus (VVMD7): the Black Currant genotype is 240:246 while the Mavri Corinthiaki genotype is 240:248. A mutation in the microsatellite sequence might be responsible for that difference. Possible consequences of such mutations are discussed

Extending Qualitative Spatial Theories with Emergent Spatial Concepts: An Automated Reasoning Approach

Qualitative Spatial Reasoning is an exciting research field of the Knowledge Representation and Reasoning paradigm whose application often requires the extension, refinement or combination of existent theories (as well as the associated calculus). This paper addresses the issue of the sound spatial interpretation of formal extensions of such theories; particularly the interpretation of the extension and the desired representational features. The paper shows how to interpret certain kinds of extensions of Region Connection Calculus (RCC) theory. We also show how to rebuild the qualitative calculus of these extensions.Junta de Andalucía TIC-606

Fatigue behaviour of laser repairing welded joints

This paper presents a fatigue study in Nd-YAG laser surface repairing welded joints in specimens of two base materials used in mould production. The tests were carried out in a servo-hydraulic machine in tension, under constant amplitude loading, with two stress ratios R = 0 and R = 0.4. Welded specimens were prepared with U notches and filled with laser welding deposits. The fatigue results are presented in the form of S-N curves obtained in welded and non-welded conditions. Complementary measurements of hardness and residual stresses profiles were carried out along the surface of laser welded specimens to understand the observed fatigue behaviour. The melted material was the weaker region, with lower values of hardness and higher tensile residual stresses, presenting also a high number of defects that are potential failure sites. The presence of such defects can explain the relatively poor fatigue strength of the laser repairing joints in comparison to base materials.http://www.sciencedirect.com/science/article/B6V2X-4N2M6PH-1/1/945a7f442fb922704c73d665abcb0b0

Mould steels repaired by laser welding

Laser-deposit welding, by using Nd-YAG, is a mould repairing process, which has the advantages relatively to the traditional methods of achieving a less change of the metal composition around the repaired zone and permitting a very accurate deposition of a small volume of the filler material in the area chosen at the work-piece surface. This paper presents a fatigue study in specimens of two base materials used in mould production (AISI H13 and P20). Filler material as well as welding parameters were analysed in order to obtain better fatigue strength. The tests were carried out under constant amplitude loading, with two stress ratios R = 0 and R = 0.4. Welded specimens were prepared with V notches and filled with laser welding deposits. The fatigue results are presented in the form of S-N curves obtained in welded and non-welded conditions. Complementary measurements of hardness profiles and SEM analysis were carried out to understanding the fatigue behaviour and failure sites. The laser-deposit material was the weaker region in both steels, due to a high level of tensile residual stresses and also to some planar defects that are potential failure sites. Fatigue crack initiation is therefore reduced and the fatigue propagation life is enhanced. A significant mean stress effect in the base material was also observed in both mould steels.http://www.sciencedirect.com/science/article/B6V2X-4S03RKY-3/1/b8b7da232b765381e7d08d9f3d7f897

Estrogen-related genes and postmenopausal osteoporosis risk

Background To date, more than 150 candidate genes related to osteoporosis have been described, but osteoporosis has increasingly been considered a polygenic disease modulated by environmental factors. It is thought that osteoporosis predisposition, pathology, and treatment response depend on the interaction between different genes or between genes and environmental factors. Objective The aim of this study was to evaluate the relationship between the presence of single nucleotide polymorphisms (SNPs) in the estrogen metabolic pathway and the development of osteoporosis and to determine whether this relationship is monogenic or whether interactions between genes exist. Materials and methods A multicentric study with 1980 postmenopausal Spanish women in fi ve Spanish communities was conducted. The women completed a specifi c questionnaire that inquired about risk factors for osteoporosis. Data on participants ’ bone mineral density were obtained with dual-energy X-ray densitometers, and genetic data were obtained from frozen peripheral blood. Results The digenic protection combinations indicated involvement of the wild-type genotype (WT) of the 3 UTR marker for the CYP19A1 gene, the IVS4 marker of the same gene, and the BMP15 and FSHR genes. Among patients who carried two or more of the genotypes considered ‘ risky ’ , the triple combination among markers of the ESR2 and NRIP1 genes with any of the two mutations of the analyzed markers of the BMP15 gene gave a mean T -score value of 2.32 0.91 ( p 0.02). Conclusion Variants of the new candidate genes ( NRIP and BMP15 ) can predispose patients to osteoporosis

Spectral fluorescence variation of pollen and spores from recent peat-forming plants

The fluorescence properties of spores and pollen grains examined under ultraviolet incident light are used to assess the maturity of sedimentary organic matter and may have other applications in relation to recent sediments, in areas such as paleoenvironmental research. In this study pollen grains and spores from 33 species common in peat ecosystems were mounted on a glass slide in accordance with standard palynologycal procedures for recent plants. The main objective of this work was to assess the variability of fluorescence spectra of pollens and spores within a single species or even within a single sample. A minimum of 10 spectra were recorded from each sample and were averaged to obtain a spectrum characteristic of each sample. Both the average scattering and the scattering in different spectral regions were calculated using the standard deviation (SD) and the coefficient of variation (CV). The effect of the preparation techniques was assessed on some samples of Ericaceae taxa. The results indicated similar spectra for alcohol-washed and distilled water-washed samples, whereas the application of an acetolysis solution caused an increase in intensity and a shift to longer wavelengths. The spectra corresponding to the Sphagnum spores had the lowest intensity of all the families studied and displayed their maxima at the lowest registered wavelengths. They often showed a peak in the red region of the spectra, causing a larger scatter in fluorescence in this region. This peak is probably the result of wax or cytoplasmic material attached to the exospore. A significant number of Ericaceae taxa had two fluorescing pollen populations: a blue one of high intensity and smaller size and a yellow-orange one of low intensity and larger size. This difference could be related to different degrees of maturity of the pollen grains. In the case of pollen grains of herbaceous, tree and bush plants the largest scatter was found in the tails of the spectra toward the blue and red regions. The decreasing trend of fluorescence intensity with the shift of the spectra toward red was not observed in the pollen and spores of fresh plants. A good correlation was found between the spectral maxima (λmax) and the red-green quotient (QR/G) regardless of the type of plant.Financial support from MICINN (CGL2009-13990-C02-01/02), MINECO (CGL2013-46458-C2-1-R) and a FPU fellowship from MEC for J. Urbanczyk are gratefully acknowledged.Peer reviewe

Cardiac Repolarization Abnormalities and Potential Evidence for Loss of Cardiac Sodium Currents on ECGs of Patients with Chagas' Heart Disease

Some individuals with Chagas disease develop right precordial lead ST segment elevation in response to an ajmaline challenge test, and the prevalence of right bundle branch block (RBBB) is also high in Chagas disease. Because these same electrocardiographic abnormalities occur in the Brugada syndrome, which involves genetically defective cardiac sodium channels, acquired damage to cardiac sodium channels may also occur in Chagas disease. We studied several conventional and advanced resting 12-lead/derived Frank-lead ECG parameters in 34 patients with Chagas -related heart disease (mean age 39 14 years) and in 34 age-/gender-matched healthy controls. All ECG recordings were of 5-10 min duration, obtained in the supine position using high fidelity hardware/software (CardioSoft, Houston, TX). Even after excluding those Chagas patients who had resting BBBs, tachycardia and/or pathologic arrhythmia (n=8), significant differences remained in multiple conventional and advanced ECG parameters between the Chagas and control groups (n=26/group), especially in their respective QT interval variability indices, maximal spatial QRS-T angles and low frequency HRV powers (p=0.0006, p=0.0015 and p=0.0314 respectively). In relation to the issue of potential damage to cardiac sodium channels, the Chagas patients had: 1) greater than or equal to twice the incidence of resting ST segment elevation in leads V1-V3 (n=10/26 vs. n=5/26) and of both leftward (n=5/26 versus n=0/26) and rightward (n=7/26 versus n=3/26) QRS axis deviation than controls; 2) significantly increased filtered (40-250 Hz) QRS interval durations (92.1 8.5 versus 85.3 plus or minus 9.0 ms, p=0.022) versus controls; and 3) significantly decreased QT and especially JT interval durations versus controls (QT interval: 387.5 plus or minus 26.4 versus 408.9 plus or minus 34.6 ms, p=0.013; JT interval: 290.5 plus or minus 26.3 versus 314.8 plus or minus 31.3 ms; p=0.0029). Heart rates and Bazett-corrected QTc/JTc intervals were not significantly different between groups. Patients with Chagas heart disease have increased cardiac repolarization abnormalities, especially by advanced ECG. Moreover, as a group, they have decreased uncorrected JT and QT interval durations and increased filtered QRS interval durations (versus age/gender-matched controls), all suggesting a potential loss of cardiac sodium channel function that might be mediated, in part, by cardiac autonomic damage. Overall findings support Brugada et al's recent hypothesis that the pathway leading to sudden death may often be similar in Chagas' disease and Brugada syndrome i.e., damage to the sodium channel (infectious/immunologic/autonomic in Chagas' genetic in Brugada) with consequent loss of sodium currents may facilitate a phase II-reentry based arrhythmic substrate for ventricular fibrillation in both conditions. In general, JT interval-related results have been underreported in the Chagas literature

Serum Vitamin D Levels Are Not Predictive of the Progression of Chronic Liver Disease in Hepatitis C Patients with Advanced Fibrosis

In animal models and human cross-sectional studies, vitamin D deficiency has been associated with liver disease progression. Vitamin D supplementation has been suggested as a treatment to prevent disease progression. We sought to evaluate the role of vitamin D levels in predicting chronic liver disease development. We conducted a nested case-control study of vitamin D levels in subjects with (cases) and without (controls) liver histologic progression or clinical decompensation over the course of the HALT-C Trial. Vitamin D levels were measured at 4 points over 45 months. 129 cases and 129 aged-matched controls were included. No difference in baseline vitamin D levels were found between cases and controls. (44.8 ng/mL vs. 44.0 ng/mL, P = 0.74). Vitamin D levels declined in cases and controls over time (P = 0.0005), however, there was no difference in the level of decline (P = 0.37). Among study subjects with diabetes mellitius, baseline vitamin D levels were higher in cases, 49.9 ng/mL, than controls, 36.3 ng/mL. (P = 0.03) In addition, baseline vitamin D levels were higher in black case subjects, 32.7 ng/mL, than in black control subjects, 25.2 ng/mL (P = 0.08) No difference in vitamin D levels was found between patients with and without progression of hepatitis C-associated liver disease over 4 years. Our data do not suggest any role for vitamin D supplementation in patients with advanced chronic hepatitis C and raise the possibility that higher vitamin D levels may be associated with disease progression

Association Between Memory B-Cells and Clinical and Immunological Features of Primary Sjögren’s Syndrome and Sicca Patients

B-cells play a pivotal role in primary Sjögren's syndrome (pSS) pathogenesis. We aim to (1) evaluate the distribution of B-lymphocyte subpopulations in pSS and Sicca patients, (2) establish cut-off points that discriminate pSS from controls, (3) evaluate the association between memory B-cells and phenotypic features in pSS. We included 57 pSS patients, 68 Sicca and 24 healthy controls. Circulating B-cells were characterized by flow cytometry as naïve and memory subsets and classified from Bm1 to Bm5. Compared to controls, pSS patients had lower percentages (29.5 vs 44.4%) and absolute numbers (47 vs 106 cells/µl) of memory B-cells. Through ROC curves, a cut-off of ≤ 58 total memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.60 for pSS, and was met by 59.6% of pSS patients, 38.8% of Sicca and 12.5% of controls. A cut-off of < 23.5 Switched-memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.54 and was met by 54.4% of pSS patients, 37.3% of Sicca and 12.5% of controls. In pSS, lower total memory B-cells count was associated with longer disease duration (14.3 vs 8.1 years, p = 0.006) and more active disease profile, as evaluated by the European League Against Rheumatism (EULAR) Sjögren's Syndrome Disease Activity Index (ESSDAI) (3.1 vs 1.4, p = 0.043). Decreased numbers of memory B-cells clearly discriminated pSS from controls and can also have prognostic value. It remains to be clarified whether Sicca patients with decreased memory B-cells represent pSS and if B-cell profiling could help in the diagnosis of pSS.info:eu-repo/semantics/publishedVersio